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The map label for this gene is gltX

Identifier: 20094194

GI number: 20094194

Start: 718688

End: 720403

Strand: Reverse

Name: gltX

Synonym: MK0757

Alternate gene names: 20094194

Gene position: 720403-718688 (Counterclockwise)

Preceding gene: 20094195

Following gene: 20094193

Centisome position: 42.5

GC content: 58.45

Gene sequence:

>1716_bases
GTGGAGGAGAAGGAACTTCGAGATCTCGTCCGCAGGTACGCTCTGGAGAACGCGGCCCGATACGGTGGGAGGGCGAATCC
CAACGCGGTGATGAAGAAGATCATGAAGGAACACGAAGAGCTACGTCCTAGGGCGAAAGAAGTCCTGAAGACCGTCAGAG
AGGTGGTCCGCGAAGTTAACAAGATGTCCGGAGAGGAAATCAGACGCGAGCTGGAAGAACTCGGAGGACCGCGCGAAGAC
GTAGCCCGGGACAAAGAAGGGCTCAAACCCCTTCCCGGAGCTGAACCGGGGAACGTTCGCCTTCGGTTCGCACCGAACCC
TTCGGGTCCCCTCCACATAGGTCACGCCCGTGCCGCAGTACTTAACGACGAATACGCTAGAAGGTACGACGGCACCTTAG
TCCTCCGCATTGAAGACACCGACCCGCGTCGCGTGGACCCTGAGGCCTACGACATGATCGAAGAGGATCTGGAGTGGCTC
GGAGTCAACATCGACGAACGGTACGTGCAGTCTAACAGGATAGAACTGTATTACATGGTTTGCGAGGAGCTGCTGGAGCG
GGAAGGGGCGTACGTTTGTACCTGTGATCCAGACGAGTTCCGGAGACTCCGCGACGTGGGGCGAGCCTGCCCGTGCCGCT
CTCGGGATAAGGAGGAGAACCTCGAGCTCTGGGAGGAGATGTTGGACGGTACTTTCTCAGAAGGAGAAGCCGTCGTACGT
GTCAAGACCGAAGTTGATCACCCGGATCCCGCGGTGAGGGAGTGGATAGCGTTCAGGATCGTCGAAGAAGAGCACCCGAT
GACGGGTTCTCGGTACCTTGTGTGGCCGACGATGAACTTCGCCGTCGCTGTGGACGACCATCTCATGAACATCACGCACG
TACTTCGTGGGAAGGATCATGAGTCCAACACACGGCGTCAGAAGTACGTGTTCGAGCATCTGGGGTGGGACACTCCGGAA
TACGTTCACTACGGGATTCTGAAGGTTGAAGGTGCCGTGCTAAGCACTTCCGAGATCCGCCGTGGAATCGATTCAGGAGA
GTACACAGGGTGGGACGACGTCCGAGTGGCTACCTTACGCGCCCTCCGACGCCGGGGTATTAAGCCGGAAGCAATACGTG
AGACTATCCTCGAGATCGGCCTGACCGACGTGGACGCGACGTTCAGCTGGGAGCATCTTTACGCTCGGAACCGTAAGATG
ATCGACCCTGAGTCTCACCGGTACTTCTTCGTCCGTGATCCTGTCGAATTACGGATCGAAGGGATGAAGGAGTCGGTCCT
GGCCCGACTGCCTCTACACCCCGATCGGGATGAGGGAGAGAGGGTCTTAATTCTACATCCTGAGAACGGCGTGGCCCGAG
CACTGTTGGACGGTGAAGACGCAGAAGACCTGTGGGAGGGCGACGTAGTTCGGCTGATGAACGCCGTCAACGTCGAGATC
GAGGAAGTCGGAGACGGATGGTTGCGAGGCAGGTATCACAGCGACGACTACCGAATCGCGAAAGAGGAAGGTGCTCAAAT
CGTCCACTGGGTACCGCCCGACCAGGCAGTACGGTGCGAGGTCGTCCGCCCGGACGGTAGCGTTGAGAGTGGATACGCCG
AAATTAACGTCGAGCGCGAACAGGCCGGCAGCACCGTCCAGTTCGAACGGCTGTACTTCGTGCGACTCGAAGAGGTCTCC
AGCGGTGGGGTAAGGGCTGTGTACGCGCATGACTAG

Upstream 100 bases:

>100_bases
CGTCTGGGTCCCCCGGGGGAGAAGGCCGTCGCCTGTCGATGATCCCGGGGCCGGAGGACGGGGGACACCCCCTCACTGAT
TATCTCGGGGAGAATGGACC

Downstream 100 bases:

>100_bases
CCCTCCCTCCAGCATAGCACTTTCCGGCGGTAAGGACAGTGTAGCCACGCTGATAGACGCCGCCGATAGGTGGAATGTTA
AGGTAGCCGTAACAGTAGTC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MEEKELRDLVRRYALENAARYGGRANPNAVMKKIMKEHEELRPRAKEVLKTVREVVREVNKMSGEEIRRELEELGGPRED
VARDKEGLKPLPGAEPGNVRLRFAPNPSGPLHIGHARAAVLNDEYARRYDGTLVLRIEDTDPRRVDPEAYDMIEEDLEWL
GVNIDERYVQSNRIELYYMVCEELLEREGAYVCTCDPDEFRRLRDVGRACPCRSRDKEENLELWEEMLDGTFSEGEAVVR
VKTEVDHPDPAVREWIAFRIVEEEHPMTGSRYLVWPTMNFAVAVDDHLMNITHVLRGKDHESNTRRQKYVFEHLGWDTPE
YVHYGILKVEGAVLSTSEIRRGIDSGEYTGWDDVRVATLRALRRRGIKPEAIRETILEIGLTDVDATFSWEHLYARNRKM
IDPESHRYFFVRDPVELRIEGMKESVLARLPLHPDRDEGERVLILHPENGVARALLDGEDAEDLWEGDVVRLMNAVNVEI
EEVGDGWLRGRYHSDDYRIAKEEGAQIVHWVPPDQAVRCEVVRPDGSVESGYAEINVEREQAGSTVQFERLYFVRLEEVS
SGGVRAVYAHD

Sequences:

>Translated_571_residues
MEEKELRDLVRRYALENAARYGGRANPNAVMKKIMKEHEELRPRAKEVLKTVREVVREVNKMSGEEIRRELEELGGPRED
VARDKEGLKPLPGAEPGNVRLRFAPNPSGPLHIGHARAAVLNDEYARRYDGTLVLRIEDTDPRRVDPEAYDMIEEDLEWL
GVNIDERYVQSNRIELYYMVCEELLEREGAYVCTCDPDEFRRLRDVGRACPCRSRDKEENLELWEEMLDGTFSEGEAVVR
VKTEVDHPDPAVREWIAFRIVEEEHPMTGSRYLVWPTMNFAVAVDDHLMNITHVLRGKDHESNTRRQKYVFEHLGWDTPE
YVHYGILKVEGAVLSTSEIRRGIDSGEYTGWDDVRVATLRALRRRGIKPEAIRETILEIGLTDVDATFSWEHLYARNRKM
IDPESHRYFFVRDPVELRIEGMKESVLARLPLHPDRDEGERVLILHPENGVARALLDGEDAEDLWEGDVVRLMNAVNVEI
EEVGDGWLRGRYHSDDYRIAKEEGAQIVHWVPPDQAVRCEVVRPDGSVESGYAEINVEREQAGSTVQFERLYFVRLEEVS
SGGVRAVYAHD
>Mature_571_residues
MEEKELRDLVRRYALENAARYGGRANPNAVMKKIMKEHEELRPRAKEVLKTVREVVREVNKMSGEEIRRELEELGGPRED
VARDKEGLKPLPGAEPGNVRLRFAPNPSGPLHIGHARAAVLNDEYARRYDGTLVLRIEDTDPRRVDPEAYDMIEEDLEWL
GVNIDERYVQSNRIELYYMVCEELLEREGAYVCTCDPDEFRRLRDVGRACPCRSRDKEENLELWEEMLDGTFSEGEAVVR
VKTEVDHPDPAVREWIAFRIVEEEHPMTGSRYLVWPTMNFAVAVDDHLMNITHVLRGKDHESNTRRQKYVFEHLGWDTPE
YVHYGILKVEGAVLSTSEIRRGIDSGEYTGWDDVRVATLRALRRRGIKPEAIRETILEIGLTDVDATFSWEHLYARNRKM
IDPESHRYFFVRDPVELRIEGMKESVLARLPLHPDRDEGERVLILHPENGVARALLDGEDAEDLWEGDVVRLMNAVNVEI
EEVGDGWLRGRYHSDDYRIAKEEGAQIVHWVPPDQAVRCEVVRPDGSVESGYAEINVEREQAGSTVQFERLYFVRLEEVS
SGGVRAVYAHD

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI62241042, Length=412, Percent_Identity=30.0970873786408, Blast_Score=200, Evalue=3e-51,
Organism=Homo sapiens, GI4826960, Length=438, Percent_Identity=29.9086757990868, Blast_Score=163, Evalue=5e-40,
Organism=Homo sapiens, GI134288884, Length=243, Percent_Identity=33.7448559670782, Blast_Score=132, Evalue=9e-31,
Organism=Escherichia coli, GI1786895, Length=521, Percent_Identity=29.9424184261036, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1788743, Length=234, Percent_Identity=35.4700854700855, Blast_Score=118, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17511007, Length=397, Percent_Identity=31.9899244332494, Blast_Score=213, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17543332, Length=344, Percent_Identity=32.8488372093023, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI71993394, Length=264, Percent_Identity=29.5454545454545, Blast_Score=110, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324742, Length=359, Percent_Identity=33.4261838440111, Blast_Score=215, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6321192, Length=504, Percent_Identity=27.9761904761905, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6324540, Length=307, Percent_Identity=30.9446254071661, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24649466, Length=411, Percent_Identity=34.0632603406326, Blast_Score=222, Evalue=5e-58,
Organism=Drosophila melanogaster, GI17864488, Length=363, Percent_Identity=31.129476584022, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24665321, Length=304, Percent_Identity=34.2105263157895, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_METKA (Q8TXB7)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614041.1
- ProteinModelPortal:   Q8TXB7
- SMR:   Q8TXB7
- GeneID:   1476858
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0757
- NMPDR:   fig|190192.1.peg.754
- HOGENOM:   HBG334108
- OMA:   MRFAPNP
- ProtClustDB:   PRK04156
- BioCyc:   MKAN190192:MK0757-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_A
- InterPro:   IPR001412
- InterPro:   IPR004526
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR022888
- InterPro:   IPR011035
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00463

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C; SSF50715 Ribosomal_L25rel

EC number: =6.1.1.17

Molecular weight: Translated: 65976; Mature: 65976

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEKELRDLVRRYALENAARYGGRANPNAVMKKIMKEHEELRPRAKEVLKTVREVVREVN
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
KMSGEEIRRELEELGGPREDVARDKEGLKPLPGAEPGNVRLRFAPNPSGPLHIGHARAAV
HCCHHHHHHHHHHHCCCHHHHHCCHHCCCCCCCCCCCCEEEEECCCCCCCEEECCHHHHH
LNDEYARRYDGTLVLRIEDTDPRRVDPEAYDMIEEDLEWLGVNIDERYVQSNRIELYYMV
HCHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEHHH
CEELLEREGAYVCTCDPDEFRRLRDVGRACPCRSRDKEENLELWEEMLDGTFSEGEAVVR
HHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEE
VKTEVDHPDPAVREWIAFRIVEEEHPMTGSRYLVWPTMNFAVAVDDHLMNITHVLRGKDH
EEECCCCCCHHHHHHHHHEEECCCCCCCCCEEEEEECCCEEEEECHHHHHHHHHHCCCCC
ESNTRRQKYVFEHLGWDTPEYVHYGILKVEGAVLSTSEIRRGIDSGEYTGWDDVRVATLR
CCHHHHHHHHHHHHCCCCHHHHEEEEEEECCCEECHHHHHCCCCCCCCCCCCHHHHHHHH
ALRRRGIKPEAIRETILEIGLTDVDATFSWEHLYARNRKMIDPESHRYFFVRDPVELRIE
HHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCEEEEECCHHHEEC
GMKESVLARLPLHPDRDEGERVLILHPENGVARALLDGEDAEDLWEGDVVRLMNAVNVEI
CHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEECCCCHHHHHCCHHHHHHHHHCCCC
EEVGDGWLRGRYHSDDYRIAKEEGAQIVHWVPPDQAVRCEVVRPDGSVESGYAEINVERE
EECCCCHHCCCEECCCCEEECCCCCEEEEECCCCCCEEEEEECCCCCCCCCEEEEEEEHH
QAGSTVQFERLYFVRLEEVSSGGVRAVYAHD
HCCCEEEEEEEEEEEEEECCCCCEEEEEECC
>Mature Secondary Structure
MEEKELRDLVRRYALENAARYGGRANPNAVMKKIMKEHEELRPRAKEVLKTVREVVREVN
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
KMSGEEIRRELEELGGPREDVARDKEGLKPLPGAEPGNVRLRFAPNPSGPLHIGHARAAV
HCCHHHHHHHHHHHCCCHHHHHCCHHCCCCCCCCCCCCEEEEECCCCCCCEEECCHHHHH
LNDEYARRYDGTLVLRIEDTDPRRVDPEAYDMIEEDLEWLGVNIDERYVQSNRIELYYMV
HCHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEHHH
CEELLEREGAYVCTCDPDEFRRLRDVGRACPCRSRDKEENLELWEEMLDGTFSEGEAVVR
HHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEE
VKTEVDHPDPAVREWIAFRIVEEEHPMTGSRYLVWPTMNFAVAVDDHLMNITHVLRGKDH
EEECCCCCCHHHHHHHHHEEECCCCCCCCCEEEEEECCCEEEEECHHHHHHHHHHCCCCC
ESNTRRQKYVFEHLGWDTPEYVHYGILKVEGAVLSTSEIRRGIDSGEYTGWDDVRVATLR
CCHHHHHHHHHHHHCCCCHHHHEEEEEEECCCEECHHHHHCCCCCCCCCCCCHHHHHHHH
ALRRRGIKPEAIRETILEIGLTDVDATFSWEHLYARNRKMIDPESHRYFFVRDPVELRIE
HHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCEEEEECCHHHEEC
GMKESVLARLPLHPDRDEGERVLILHPENGVARALLDGEDAEDLWEGDVVRLMNAVNVEI
CHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEECCCCHHHHHCCHHHHHHHHHCCCC
EEVGDGWLRGRYHSDDYRIAKEEGAQIVHWVPPDQAVRCEVVRPDGSVESGYAEINVERE
EECCCCHHCCCEECCCCEEECCCCCEEEEECCCCCCEEEEEECCCCCCCCCEEEEEEEHH
QAGSTVQFERLYFVRLEEVSSGGVRAVYAHD
HCCCEEEEEEEEEEEEEECCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014