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The map label for this gene is RpoC_1 [H]

Identifier: 20094122

GI number: 20094122

Start: 642269

End: 643558

Strand: Reverse

Name: RpoC_1 [H]

Synonym: MK0684

Alternate gene names: 20094122

Gene position: 643558-642269 (Counterclockwise)

Preceding gene: 20094123

Following gene: 20094121

Centisome position: 37.97

GC content: 63.02

Gene sequence:

>1290_bases
ATGAACGTGGAGGAGCTGCTCGAAGAGGTCTGTAAGGAGGAGGACGTGGAGCTCCCGGACTCGGTACACCGAGAGATCGT
CGAGAAGGTGTCGGAGGACGAGGAGCTCAAGGACGCCGACGAAGAAACCGTGCGGAAGGTCCTCCGGGCGCTCGTACGCA
ACTACAAGTGCCGACAGATCGAGCGGGGCGACGCGGTGGGCGTTCTGGCGGCTCAGTCGATGTGTGAGCCGGCGACGCAG
ATGACCATGCGTACGTTCCACCTGGCCGGTGTGGCGGAGATCGACATCACCACCGGTATGCCGGCGCTGGAGCGTGTCCT
TTCTCTCCCCTATCACGGTCCCCCGACGTGGGTCATCGAACTGAGACTCAAGGAACCGTACCGCAGCGATCGGGAGAAGG
CCGAGGGAGTGGCGCGAAAGATCGAGGAGACGCGGTTCGAGGAACTCACGGAGTACATCGAGGTGAACCCGGACGAGTGC
GCCATCGAGATCGGCCTGGATGAGGAGCGGATAGAGGAACACGGGATCGACGAAGACCAGCTCGTGGCCTTCGTCCGGGA
GCACACCGGCGGGGCGTGTCACCTCGAGGGAGATGTCCTCAAGGTGACGCTCGCGGACGTGGAGTCGGCATCGGAGCTGG
CCGAGATCGCGAACGACCTCCGCGAGGCCGTAGTGAAGGGGATCGAGGGGGTACTCCACGCCTCCGTCCAGTACGATGAG
GACGAGGAGGAATACTACATCCTTGTCAGAGGTAGTCCTGAGGGAGCCCCGGGACGGCCTCCTAGGTGGATCTCCCAGCG
CGCCAGGAACAGCAAGGTGGCGAAGCGCGCCTACCTGATGCTGCAGCTGATGCAGTTGGACGAGGTCGACGCTACGCGGG
TGAACACGAACGACCCGAAGATAGTGGACGTGCTCCTCGGGGTGGAGGCCGGGCGGAACGCGATCATCGAGCAGCTCGAG
GAGGTGTACGAGAGCCAGGGGATCTCCGTAGACCCGCGCCACTTCATGCTCATCAGCGACATGATGTCGTTCTGGGGCGA
GCTGCGTAGCGTACGGTCCGTCGGCGGTTACTCGGTGATGGAGCTGAAGGGCAGCCCGCTCACGAAGGCCGCCTTCGAGT
ACGTATCGAGGGTCCTCACCGAAACGGCGACGTCGGGTGACGTCGAGGAGCTGCGTGGAGTGCTCGAGAACATCATCGTG
GGTAAGCCGGTACCGGTGGGTACCGGTACGGTGGAGCTCACAGTGGATTACTCCAAGTTCAAGGAGGGAGAAGAGGAAGG
GGAGAGCTGA

Upstream 100 bases:

>100_bases
CCGCCAAGAAGTGGGGCTTGAACCTGGACGTCGAGAAGGCGCTGAAGGTCGTGAAGGAAGTGGTGCTGGAGGAAGAGAGA
AGAGTGAGGGGAGGCGCGGG

Downstream 100 bases:

>100_bases
ACTGGGCCGCTTTCCTTCCCATCACCCCATGGGACTACGATCGCTTACACCCACTAGCGGCACGGGCGCCGGTAGGTACC
CGCGGAGGTAACTCGGTACC

Product: DNA-directed RNA polymerase subunit A''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNVEELLEEVCKEEDVELPDSVHREIVEKVSEDEELKDADEETVRKVLRALVRNYKCRQIERGDAVGVLAAQSMCEPATQ
MTMRTFHLAGVAEIDITTGMPALERVLSLPYHGPPTWVIELRLKEPYRSDREKAEGVARKIEETRFEELTEYIEVNPDEC
AIEIGLDEERIEEHGIDEDQLVAFVREHTGGACHLEGDVLKVTLADVESASELAEIANDLREAVVKGIEGVLHASVQYDE
DEEEYYILVRGSPEGAPGRPPRWISQRARNSKVAKRAYLMLQLMQLDEVDATRVNTNDPKIVDVLLGVEAGRNAIIEQLE
EVYESQGISVDPRHFMLISDMMSFWGELRSVRSVGGYSVMELKGSPLTKAAFEYVSRVLTETATSGDVEELRGVLENIIV
GKPVPVGTGTVELTVDYSKFKEGEEEGES

Sequences:

>Translated_429_residues
MNVEELLEEVCKEEDVELPDSVHREIVEKVSEDEELKDADEETVRKVLRALVRNYKCRQIERGDAVGVLAAQSMCEPATQ
MTMRTFHLAGVAEIDITTGMPALERVLSLPYHGPPTWVIELRLKEPYRSDREKAEGVARKIEETRFEELTEYIEVNPDEC
AIEIGLDEERIEEHGIDEDQLVAFVREHTGGACHLEGDVLKVTLADVESASELAEIANDLREAVVKGIEGVLHASVQYDE
DEEEYYILVRGSPEGAPGRPPRWISQRARNSKVAKRAYLMLQLMQLDEVDATRVNTNDPKIVDVLLGVEAGRNAIIEQLE
EVYESQGISVDPRHFMLISDMMSFWGELRSVRSVGGYSVMELKGSPLTKAAFEYVSRVLTETATSGDVEELRGVLENIIV
GKPVPVGTGTVELTVDYSKFKEGEEEGES
>Mature_429_residues
MNVEELLEEVCKEEDVELPDSVHREIVEKVSEDEELKDADEETVRKVLRALVRNYKCRQIERGDAVGVLAAQSMCEPATQ
MTMRTFHLAGVAEIDITTGMPALERVLSLPYHGPPTWVIELRLKEPYRSDREKAEGVARKIEETRFEELTEYIEVNPDEC
AIEIGLDEERIEEHGIDEDQLVAFVREHTGGACHLEGDVLKVTLADVESASELAEIANDLREAVVKGIEGVLHASVQYDE
DEEEYYILVRGSPEGAPGRPPRWISQRARNSKVAKRAYLMLQLMQLDEVDATRVNTNDPKIVDVLLGVEAGRNAIIEQLE
EVYESQGISVDPRHFMLISDMMSFWGELRSVRSVGGYSVMELKGSPLTKAAFEYVSRVLTETATSGDVEELRGVLENIIV
GKPVPVGTGTVELTVDYSKFKEGEEEGES

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0086

COG function: function code K; DNA-directed RNA polymerase, beta' subunit/160 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta' chain family [H]

Homologues:

Organism=Homo sapiens, GI39725938, Length=384, Percent_Identity=32.2916666666667, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI4505939, Length=416, Percent_Identity=25.7211538461538, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI103471997, Length=125, Percent_Identity=33.6, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25145495, Length=379, Percent_Identity=30.3430079155673, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI71987878, Length=416, Percent_Identity=25, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320061, Length=405, Percent_Identity=28.641975308642, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324690, Length=445, Percent_Identity=27.8651685393258, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6324917, Length=125, Percent_Identity=32.8, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281360912, Length=385, Percent_Identity=29.6103896103896, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI17530899, Length=421, Percent_Identity=25.6532066508314, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI17647875, Length=125, Percent_Identity=34.4, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007081
- InterPro:   IPR012757 [H]

Pfam domain/function: PF04998 RNA_pol_Rpb1_5 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 48035; Mature: 48035

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVEELLEEVCKEEDVELPDSVHREIVEKVSEDEELKDADEETVRKVLRALVRNYKCRQI
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHCCCHHHC
ERGDAVGVLAAQSMCEPATQMTMRTFHLAGVAEIDITTGMPALERVLSLPYHGPPTWVIE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCCCCEEEE
LRLKEPYRSDREKAEGVARKIEETRFEELTEYIEVNPDECAIEIGLDEERIEEHGIDEDQ
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCHHH
LVAFVREHTGGACHLEGDVLKVTLADVESASELAEIANDLREAVVKGIEGVLHASVQYDE
HHHHHHHCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DEEEYYILVRGSPEGAPGRPPRWISQRARNSKVAKRAYLMLQLMQLDEVDATRVNTNDPK
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
IVDVLLGVEAGRNAIIEQLEEVYESQGISVDPRHFMLISDMMSFWGELRSVRSVGGYSVM
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
ELKGSPLTKAAFEYVSRVLTETATSGDVEELRGVLENIIVGKPVPVGTGTVELTVDYSKF
EECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHH
KEGEEEGES
CCCCCCCCC
>Mature Secondary Structure
MNVEELLEEVCKEEDVELPDSVHREIVEKVSEDEELKDADEETVRKVLRALVRNYKCRQI
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHCCCHHHC
ERGDAVGVLAAQSMCEPATQMTMRTFHLAGVAEIDITTGMPALERVLSLPYHGPPTWVIE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCCCCEEEE
LRLKEPYRSDREKAEGVARKIEETRFEELTEYIEVNPDECAIEIGLDEERIEEHGIDEDQ
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCHHH
LVAFVREHTGGACHLEGDVLKVTLADVESASELAEIANDLREAVVKGIEGVLHASVQYDE
HHHHHHHCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DEEEYYILVRGSPEGAPGRPPRWISQRARNSKVAKRAYLMLQLMQLDEVDATRVNTNDPK
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
IVDVLLGVEAGRNAIIEQLEEVYESQGISVDPRHFMLISDMMSFWGELRSVRSVGGYSVM
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
ELKGSPLTKAAFEYVSRVLTETATSGDVEELRGVLENIIVGKPVPVGTGTVELTVDYSKF
EECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHH
KEGEEEGES
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]