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The map label for this gene is ArgH

Identifier: 20094060

GI number: 20094060

Start: 580781

End: 582307

Strand: Reverse

Name: ArgH

Synonym: MK0622

Alternate gene names: 20094060

Gene position: 582307-580781 (Counterclockwise)

Preceding gene: 20094065

Following gene: 20094059

Centisome position: 34.36

GC content: 70.01

Gene sequence:

>1527_bases
TTGGCACTGGGGAGACCGTCCTACCGGGGCGGTCGCGTCTCCGGCGGCCGCGACCCGGACTTCGCGGAATTGTCATCGTC
GCTGGAGCAGGACCGGGAGATCTTCCACTGCGACGTGTGGAACTCGGTGGTCCACGCGGTGTCACTGTGGGAGGCCGGGA
GGATAGATCGCTCAACCGCGGCGGGAATCGTTGAGGGTCTCGTAACGGTTCTCGAGGAAGGTCCCGACCGGCTGCCCGAG
GACGCCGAGGACGTCCACGAGGCCATAGAGTCGAGGCTACACGAAGTGGTCGGCGAGGAAGCCGGATGGCTGCAGCTCGG
CAGGAGCAGGAACGACCAGGTGGCGACGAGCGTGCGGATGCGGCTGAGGGAGCGCGCGCTGGACCTGTCGCGCGAGCTGG
TCGGGCTCGGGAGGGCGCTGCTGGACCTGGCCCGGGAGCACGCCGAGGTACCGATCGCCGGGTACACGCACCTGAAGCGG
GCGCAGCCGTGCACGATCGGGTTCTGGATGTCCACCTACGCGGCGGCGGTCGCGCGTTCCGCCCGGGGGTTGCTCAGGGT
CCCGGGGATGGACGAGTGCCCGCTGGGGTGCTCCGCGTTCCCGGGATCGACGGTTCCTGTGGACAGGCACCGGGAGGCCG
CGTTGCTGGGCTTCCGGAAACCGGCGCGGCACTGCGGTGAGGCCACGGCGTTGAGGGGACCGATCCTGGAGTTCCTCGGG
AGACTGGCCACCGCGGCCTCGGAGCTGACCAGGTTGGCCGGGGACCTGATACAGCTGTGCTCCGACGAGCTCGGGGTGGT
GGAGCCCCCGGACGAGCTGTCGTCGACGAGCTCGGTGATGCCGCACAAGAAGAACCCGGACGCGCTGGAGCTCGTCCGGG
CCGAGCTGACCGTAGTCGCGGGACTGAAGGGGCTCGGGGACGCCGTCCACGGTAAGCTTCCGATGTTCTACAACCGGGAC
CTCCAGGTGTTGAACGGCCTGCTATGGGACTCGGTGAACCGGTTCGAGCTGTGCGTGCGGGTGCTCCGTAAGGTGGTCGA
GGGACTGGACGTGGACGAGGAGGCGGCCCGGGGTACCGTCCTGGGATCGCACGCGGCCGCCGTCGACCTGGCGGAGCTCG
TGGCGGAGCGGGCGGGACTGACGTTCCGGGAGGCGCACAAGGTCGTGGGCAGGGTCTCGGCGCGGCTGGACCGCGAAGGA
GTTCCCATGAGCCCCGAGCGGGCGGATCGGGTGGTCGAGGAGCTCGAACGGGAAGGGGTGAAGCTGCGGGTCGAGGAGGT
CCGCGAAGTCCTGAGTCTGAAGAGGACGCTCCGGCGCCCCGTGGAGGGGTCCACCGATCCCGGACGACTGGAAGTGACCT
TGGATCGACTGCGGGCGGAGCTCGCGGCCGCCGAGCGCCTGGAGGGCACGTGGCGCGGGAGGATCGAGCGGGCCCTGCGC
GCGACCGAGGCGGCGGTCAACCGGCTGGGCGTGGAGGGGTTCGCGGAGGTCTACCGGGGGTACTGGGATGGAGAAGCTCC
CGGGTAA

Upstream 100 bases:

>100_bases
TTAACCGTAGGGGAACGACGTGCACGTCAGCCGCGACGTATGTGCCCATCCCCCCGGGATGACCGGAGTTACCCCCACCG
ACCCTGTGGGTGTGACGAGA

Downstream 100 bases:

>100_bases
GGCCCCCGCCTACGTGTGCCAGGAGTGCGAGCTCGTCACGCCGCTGCGTCGGTGTCCGGAGTGTGGTGAAGGTACGGACC
CGCTACGCGTGACCCCTCCG

Product: argininosuccinate lyase

Products: NA

Alternate protein names: ASAL; Arginosuccinase

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MALGRPSYRGGRVSGGRDPDFAELSSSLEQDREIFHCDVWNSVVHAVSLWEAGRIDRSTAAGIVEGLVTVLEEGPDRLPE
DAEDVHEAIESRLHEVVGEEAGWLQLGRSRNDQVATSVRMRLRERALDLSRELVGLGRALLDLAREHAEVPIAGYTHLKR
AQPCTIGFWMSTYAAAVARSARGLLRVPGMDECPLGCSAFPGSTVPVDRHREAALLGFRKPARHCGEATALRGPILEFLG
RLATAASELTRLAGDLIQLCSDELGVVEPPDELSSTSSVMPHKKNPDALELVRAELTVVAGLKGLGDAVHGKLPMFYNRD
LQVLNGLLWDSVNRFELCVRVLRKVVEGLDVDEEAARGTVLGSHAAAVDLAELVAERAGLTFREAHKVVGRVSARLDREG
VPMSPERADRVVEELEREGVKLRVEEVREVLSLKRTLRRPVEGSTDPGRLEVTLDRLRAELAAAERLEGTWRGRIERALR
ATEAAVNRLGVEGFAEVYRGYWDGEAPG

Sequences:

>Translated_508_residues
MALGRPSYRGGRVSGGRDPDFAELSSSLEQDREIFHCDVWNSVVHAVSLWEAGRIDRSTAAGIVEGLVTVLEEGPDRLPE
DAEDVHEAIESRLHEVVGEEAGWLQLGRSRNDQVATSVRMRLRERALDLSRELVGLGRALLDLAREHAEVPIAGYTHLKR
AQPCTIGFWMSTYAAAVARSARGLLRVPGMDECPLGCSAFPGSTVPVDRHREAALLGFRKPARHCGEATALRGPILEFLG
RLATAASELTRLAGDLIQLCSDELGVVEPPDELSSTSSVMPHKKNPDALELVRAELTVVAGLKGLGDAVHGKLPMFYNRD
LQVLNGLLWDSVNRFELCVRVLRKVVEGLDVDEEAARGTVLGSHAAAVDLAELVAERAGLTFREAHKVVGRVSARLDREG
VPMSPERADRVVEELEREGVKLRVEEVREVLSLKRTLRRPVEGSTDPGRLEVTLDRLRAELAAAERLEGTWRGRIERALR
ATEAAVNRLGVEGFAEVYRGYWDGEAPG
>Mature_507_residues
ALGRPSYRGGRVSGGRDPDFAELSSSLEQDREIFHCDVWNSVVHAVSLWEAGRIDRSTAAGIVEGLVTVLEEGPDRLPED
AEDVHEAIESRLHEVVGEEAGWLQLGRSRNDQVATSVRMRLRERALDLSRELVGLGRALLDLAREHAEVPIAGYTHLKRA
QPCTIGFWMSTYAAAVARSARGLLRVPGMDECPLGCSAFPGSTVPVDRHREAALLGFRKPARHCGEATALRGPILEFLGR
LATAASELTRLAGDLIQLCSDELGVVEPPDELSSTSSVMPHKKNPDALELVRAELTVVAGLKGLGDAVHGKLPMFYNRDL
QVLNGLLWDSVNRFELCVRVLRKVVEGLDVDEEAARGTVLGSHAAAVDLAELVAERAGLTFREAHKVVGRVSARLDREGV
PMSPERADRVVEELEREGVKLRVEEVREVLSLKRTLRRPVEGSTDPGRLEVTLDRLRAELAAAERLEGTWRGRIERALRA
TEAAVNRLGVEGFAEVYRGYWDGEAPG

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily

Homologues:

Organism=Homo sapiens, GI31541964, Length=400, Percent_Identity=35.5, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI68303542, Length=400, Percent_Identity=35.5, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI68303547, Length=399, Percent_Identity=33.8345864661654, Blast_Score=192, Evalue=8e-49,
Organism=Homo sapiens, GI68303549, Length=399, Percent_Identity=33.5839598997494, Blast_Score=187, Evalue=2e-47,
Organism=Escherichia coli, GI1790398, Length=448, Percent_Identity=33.2589285714286, Blast_Score=216, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6321806, Length=471, Percent_Identity=31.4225053078556, Blast_Score=188, Evalue=2e-48,
Organism=Drosophila melanogaster, GI221473854, Length=405, Percent_Identity=32.0987654320988, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI78706858, Length=405, Percent_Identity=32.0987654320988, Blast_Score=176, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARLY_METKA (Q8TXN9)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613907.1
- ProteinModelPortal:   Q8TXN9
- SMR:   Q8TXN9
- GeneID:   1476723
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0622
- NMPDR:   fig|190192.1.peg.620
- HOGENOM:   HBG539632
- OMA:   EDIHTVI
- BioCyc:   MKAN190192:MK0622-MONOMER
- BRENDA:   4.3.2.1
- GO:   GO:0005737
- HAMAP:   MF_00006
- InterPro:   IPR009049
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR008948
- InterPro:   IPR022761
- PANTHER:   PTHR11444:SF3
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00838

Pfam domain/function: PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.2.1

Molecular weight: Translated: 55636; Mature: 55505

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALGRPSYRGGRVSGGRDPDFAELSSSLEQDREIFHCDVWNSVVHAVSLWEAGRIDRSTA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHCCCCCHHHH
AGIVEGLVTVLEEGPDRLPEDAEDVHEAIESRLHEVVGEEAGWLQLGRSRNDQVATSVRM
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHH
RLRERALDLSRELVGLGRALLDLAREHAEVPIAGYTHLKRAQPCTIGFWMSTYAAAVARS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHH
ARGLLRVPGMDECPLGCSAFPGSTVPVDRHREAALLGFRKPARHCGEATALRGPILEFLG
HCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHCCHHHHHHHHHHHHH
RLATAASELTRLAGDLIQLCSDELGVVEPPDELSSTSSVMPHKKNPDALELVRAELTVVA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHCCCCCCCCCHHHHHHHHHHHHHH
GLKGLGDAVHGKLPMFYNRDLQVLNGLLWDSVNRFELCVRVLRKVVEGLDVDEEAARGTV
HHHHHHHHHHCCCCHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHCCCC
LGSHAAAVDLAELVAERAGLTFREAHKVVGRVSARLDREGVPMSPERADRVVEELEREGV
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
KLRVEEVREVLSLKRTLRRPVEGSTDPGRLEVTLDRLRAELAAAERLEGTWRGRIERALR
EEEHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATEAAVNRLGVEGFAEVYRGYWDGEAPG
HHHHHHHHHCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ALGRPSYRGGRVSGGRDPDFAELSSSLEQDREIFHCDVWNSVVHAVSLWEAGRIDRSTA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHCCCCCHHHH
AGIVEGLVTVLEEGPDRLPEDAEDVHEAIESRLHEVVGEEAGWLQLGRSRNDQVATSVRM
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHH
RLRERALDLSRELVGLGRALLDLAREHAEVPIAGYTHLKRAQPCTIGFWMSTYAAAVARS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHH
ARGLLRVPGMDECPLGCSAFPGSTVPVDRHREAALLGFRKPARHCGEATALRGPILEFLG
HCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHCCHHHHHHHHHHHHH
RLATAASELTRLAGDLIQLCSDELGVVEPPDELSSTSSVMPHKKNPDALELVRAELTVVA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHCCCCCCCCCHHHHHHHHHHHHHH
GLKGLGDAVHGKLPMFYNRDLQVLNGLLWDSVNRFELCVRVLRKVVEGLDVDEEAARGTV
HHHHHHHHHHCCCCHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHCCCC
LGSHAAAVDLAELVAERAGLTFREAHKVVGRVSARLDREGVPMSPERADRVVEELEREGV
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
KLRVEEVREVLSLKRTLRRPVEGSTDPGRLEVTLDRLRAELAAAERLEGTWRGRIERALR
EEEHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATEAAVNRLGVEGFAEVYRGYWDGEAPG
HHHHHHHHHCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014