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The map label for this gene is TyrS

Identifier: 20093954

GI number: 20093954

Start: 479449

End: 480423

Strand: Reverse

Name: TyrS

Synonym: MK0516

Alternate gene names: 20093954

Gene position: 480423-479449 (Counterclockwise)

Preceding gene: 20093955

Following gene: 20093953

Centisome position: 28.34

GC content: 57.33

Gene sequence:

>975_bases
GTGGACGTCGAGGAGAGGTTGAAACTCGTAACCCGAAACGCCGTCGAGGTCGTAACCGAGGAGGAGCTCCGACAACTTCT
CGAGGAAAAAGAAGAACCCGTAGCGTACGTCGGGTTCGAGCCTTCGGGTAAAGTTCACCTCGGCCACAAGTTGGTCATCG
ACAAGATGATCGACCTCCAAGAGGCCGGCTTCCACGTGATCATACTCCTCGCGGATCTCCACGCGTATCTCAACGAGAAA
GGTACGTTAGAGGAAGTACGTGAGCTGGCGGATTACAACCGCCGATGCTTCCTGGCGATGGGTTTAGACCCGAACAAGAC
CGAGTTCGTCCTCGGGTCGGAGTTTCAGCTGGACGAAGACTACGCGCTGGACGTGTACCGGATGGCTCGTCATACTACCA
TGCGCCGAGCCCGACGCAGCATGGATATGATCGCTAGGAGCGAGGAAAACCCTCCGGTATCCCAGGTTGTCTACCCTCTT
ATGCAGGCACTGGACATCGTGCACCTAAACGTCGATCTAGCGGTCGGTGGGCTCGAACAGCGCAAAATCCACATGTTGGC
CCGGGACGTGCTCCCCAAGCTAGGTTACGACTCGCCGACGTGCTTACACACCCCGATCATCCACGGGCTCGACGGAGACG
AGAAGATGTCTTCCAGCAAGAACAACTTCATCGCCGTCGACGACGAGCCGGAGGTCATCCGGGAAAAGCTTCGAAAGGCG
TACTGTCCGGCCCGCGAAGCCGAAGGTAACCCGATCCTCGAGATCTATCGGTACTTCATATTCCGGGAGTACGATGAGGT
CACGATCGAACGTCCGGAGAAATACGGTGGCGATGTCACCTACACCAGTTACGAAGAGCTGGAGCGGGACTTCGTCGACG
GCGAGCTCCATCCGCTCGACCTGAAAGAGAACGCCGCGGGGTACCTCTCCGAGATCCTGAAACCCGTAAGGAAAGCCGTG
AGCGCACCATCATGA

Upstream 100 bases:

>100_bases
TCGGACAGCGCGTGTACGTGATTCTGCTGAAAGGTGTCGCCGTAGTGGTTCCTGAGGAATACCTTTAACGGACGCAGGAC
GCCGCCAGGGGGTCTCGTCC

Downstream 100 bases:

>100_bases
AGGGGTTGATGGCGACTCGCGACTCAGAGGCGAGCTCGTCCTCAAACTGCCCGCTGAAGTAGCCTCCGGAGTTCTGTGGG
AGGGTGGACCCAAAGGTTTC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MDVEERLKLVTRNAVEVVTEEELRQLLEEKEEPVAYVGFEPSGKVHLGHKLVIDKMIDLQEAGFHVIILLADLHAYLNEK
GTLEEVRELADYNRRCFLAMGLDPNKTEFVLGSEFQLDEDYALDVYRMARHTTMRRARRSMDMIARSEENPPVSQVVYPL
MQALDIVHLNVDLAVGGLEQRKIHMLARDVLPKLGYDSPTCLHTPIIHGLDGDEKMSSSKNNFIAVDDEPEVIREKLRKA
YCPAREAEGNPILEIYRYFIFREYDEVTIERPEKYGGDVTYTSYEELERDFVDGELHPLDLKENAAGYLSEILKPVRKAV
SAPS

Sequences:

>Translated_324_residues
MDVEERLKLVTRNAVEVVTEEELRQLLEEKEEPVAYVGFEPSGKVHLGHKLVIDKMIDLQEAGFHVIILLADLHAYLNEK
GTLEEVRELADYNRRCFLAMGLDPNKTEFVLGSEFQLDEDYALDVYRMARHTTMRRARRSMDMIARSEENPPVSQVVYPL
MQALDIVHLNVDLAVGGLEQRKIHMLARDVLPKLGYDSPTCLHTPIIHGLDGDEKMSSSKNNFIAVDDEPEVIREKLRKA
YCPAREAEGNPILEIYRYFIFREYDEVTIERPEKYGGDVTYTSYEELERDFVDGELHPLDLKENAAGYLSEILKPVRKAV
SAPS
>Mature_324_residues
MDVEERLKLVTRNAVEVVTEEELRQLLEEKEEPVAYVGFEPSGKVHLGHKLVIDKMIDLQEAGFHVIILLADLHAYLNEK
GTLEEVRELADYNRRCFLAMGLDPNKTEFVLGSEFQLDEDYALDVYRMARHTTMRRARRSMDMIARSEENPPVSQVVYPL
MQALDIVHLNVDLAVGGLEQRKIHMLARDVLPKLGYDSPTCLHTPIIHGLDGDEKMSSSKNNFIAVDDEPEVIREKLRKA
YCPAREAEGNPILEIYRYFIFREYDEVTIERPEKYGGDVTYTSYEELERDFVDGELHPLDLKENAAGYLSEILKPVRKAV
SAPS

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI4507947, Length=326, Percent_Identity=37.1165644171779, Blast_Score=201, Evalue=9e-52,
Organism=Escherichia coli, GI1789786, Length=225, Percent_Identity=23.1111111111111, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71992775, Length=321, Percent_Identity=33.9563862928349, Blast_Score=141, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6321624, Length=333, Percent_Identity=36.6366366366366, Blast_Score=205, Evalue=1e-53,
Organism=Drosophila melanogaster, GI21357223, Length=327, Percent_Identity=34.2507645259939, Blast_Score=197, Evalue=7e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYY_METKA (Q8TXZ2)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613801.1
- ProteinModelPortal:   Q8TXZ2
- SMR:   Q8TXZ2
- GeneID:   1476617
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0516
- NMPDR:   fig|190192.1.peg.514
- HOGENOM:   HBG330667
- OMA:   DVAVGGM
- ProtClustDB:   PRK08560
- BioCyc:   MKAN190192:MK0516-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02008
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002307
- InterPro:   IPR015624
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11946:SF8
- PIRSF:   PIRSF006588
- PRINTS:   PR01040
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 37153; Mature: 37153

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 36-36 BINDING 158-158 BINDING 162-162 BINDING 165-165 BINDING 180-180 BINDING 218-218

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVEERLKLVTRNAVEVVTEEELRQLLEEKEEPVAYVGFEPSGKVHLGHKLVIDKMIDLQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEECHHHHHHHHHHHH
EAGFHVIILLADLHAYLNEKGTLEEVRELADYNRRCFLAMGLDPNKTEFVLGSEFQLDED
HCCCEEEEHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCCCCCC
YALDVYRMARHTTMRRARRSMDMIARSEENPPVSQVVYPLMQALDIVHLNVDLAVGGLEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCHH
RKIHMLARDVLPKLGYDSPTCLHTPIIHGLDGDEKMSSSKNNFIAVDDEPEVIREKLRKA
HHHHHHHHHHHHHCCCCCCCEECCCHHCCCCCHHHHHCCCCCEEEECCCHHHHHHHHHHH
YCPAREAEGNPILEIYRYFIFREYDEVTIERPEKYGGDVTYTSYEELERDFVDGELHPLD
CCCCCCCCCCHHHHHHHHHHHHHHCHHEEECCHHCCCCCEECCHHHHHHHHCCCCCCCCC
LKENAAGYLSEILKPVRKAVSAPS
CCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDVEERLKLVTRNAVEVVTEEELRQLLEEKEEPVAYVGFEPSGKVHLGHKLVIDKMIDLQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEECHHHHHHHHHHHH
EAGFHVIILLADLHAYLNEKGTLEEVRELADYNRRCFLAMGLDPNKTEFVLGSEFQLDED
HCCCEEEEHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCCCCCC
YALDVYRMARHTTMRRARRSMDMIARSEENPPVSQVVYPLMQALDIVHLNVDLAVGGLEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCHH
RKIHMLARDVLPKLGYDSPTCLHTPIIHGLDGDEKMSSSKNNFIAVDDEPEVIREKLRKA
HHHHHHHHHHHHHCCCCCCCEECCCHHCCCCCHHHHHCCCCCEEEECCCHHHHHHHHHHH
YCPAREAEGNPILEIYRYFIFREYDEVTIERPEKYGGDVTYTSYEELERDFVDGELHPLD
CCCCCCCCCCHHHHHHHHHHHHHHCHHEEECCHHCCCCCEECCHHHHHHHHCCCCCCCCC
LKENAAGYLSEILKPVRKAVSAPS
CCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014