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The map label for this gene is DapF

Identifier: 20093872

GI number: 20093872

Start: 405568

End: 406407

Strand: Reverse

Name: DapF

Synonym: MK0434

Alternate gene names: 20093872

Gene position: 406407-405568 (Counterclockwise)

Preceding gene: 20093873

Following gene: 20093871

Centisome position: 23.98

GC content: 60.24

Gene sequence:

>840_bases
ATGCAATTCTGGAAAGTCCATGGCGCTCGGAACGACTTCGTCTTAGTCGATGAAACAGAGGAAGAAGTGGTTCCCGAGAG
TGATAAGCCCGACTTCGCGAGGTGGGCGTGTGACCGGCGGTCCGGCGTAGGTGCCGACGGCGTGGTGTTCATACGTTCCG
ATCCTCCGAGCGTAGAGATGAGGATATTCAACCGGGACGGCTCCGAGGCCGAGTTCTGTGGGAACGCCGCGCGGTGTGTC
GTGAAGTACGTGACGGAGGTACGGGGAGAGAACGTTAAGATCTTGAGAACGTTATCCGGAGCCCATCGGGTTGAGGTGCA
GGGAGGTTGGATCGCCGTCGAGGTGCCCGAAGCCGAGATCAAGAAGGTGGTCGAGTTAGGTTACGAGGTGGACGCTGGTG
TCCCCCATTTCGTCCGGCTCACTGAGCGGGACCCGATTCATGATTTCGGAGGCCTGACCGACGAGGCCAAGACTATTTTT
TCCGAATATGAACCGAAGGGAGGCGTTAACGTAACCTACGCAGCACCTTCGGTGGACGAGCTGAGAGTCAGGACGTTCGA
GCGTGGCGTCGGCTGGACGCCGGCTTGCGGGTCCGGAGTCGTCGCGGCTTCTCTGGTGTACTCAGAGATCTTCGGACCGT
TCGAGGAAGTGAGTGTCCGGACGGCCGGAGGCTGCCTGCGCGTGTCGCTTTCCGACGGACCGCTGCTGATAGGGAGGGCT
GAGATCGTGTACAAGGGTGAACTGCGGGGTGATTGGCGTGAGAATACTGATCACCAACGACGACGGCATAGCCTCTCCCG
GTCTCCGAGCGGCCGTCCGCGCCTGCAGGAGTGTCGGTGA

Upstream 100 bases:

>100_bases
CGAGTTGTCAAAGACGTGGACAAGAAGACCGAGGCTAAGACCCAGATAGTCGATGCGCTCCGCTGCCTCTGAAACCCTGC
CACTACCTGGGGGAAGTACC

Downstream 100 bases:

>100_bases
AGTTACCGTAGTAGCTCCCGCGACGCAGCAAAGTGGTGTAGGGCGCAGTATCTCTTTACTCGAGCCAGTGCGGGTGGAAG
AGATAGAGGTAGAAGGCGTC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MQFWKVHGARNDFVLVDETEEEVVPESDKPDFARWACDRRSGVGADGVVFIRSDPPSVEMRIFNRDGSEAEFCGNAARCV
VKYVTEVRGENVKILRTLSGAHRVEVQGGWIAVEVPEAEIKKVVELGYEVDAGVPHFVRLTERDPIHDFGGLTDEAKTIF
SEYEPKGGVNVTYAAPSVDELRVRTFERGVGWTPACGSGVVAASLVYSEIFGPFEEVSVRTAGGCLRVSLSDGPLLIGRA
EIVYKGELRGDWRENTDHQRRRHSLSRSPSGRPRLQECR

Sequences:

>Translated_279_residues
MQFWKVHGARNDFVLVDETEEEVVPESDKPDFARWACDRRSGVGADGVVFIRSDPPSVEMRIFNRDGSEAEFCGNAARCV
VKYVTEVRGENVKILRTLSGAHRVEVQGGWIAVEVPEAEIKKVVELGYEVDAGVPHFVRLTERDPIHDFGGLTDEAKTIF
SEYEPKGGVNVTYAAPSVDELRVRTFERGVGWTPACGSGVVAASLVYSEIFGPFEEVSVRTAGGCLRVSLSDGPLLIGRA
EIVYKGELRGDWRENTDHQRRRHSLSRSPSGRPRLQECR
>Mature_279_residues
MQFWKVHGARNDFVLVDETEEEVVPESDKPDFARWACDRRSGVGADGVVFIRSDPPSVEMRIFNRDGSEAEFCGNAARCV
VKYVTEVRGENVKILRTLSGAHRVEVQGGWIAVEVPEAEIKKVVELGYEVDAGVPHFVRLTERDPIHDFGGLTDEAKTIF
SEYEPKGGVNVTYAAPSVDELRVRTFERGVGWTPACGSGVVAASLVYSEIFGPFEEVSVRTAGGCLRVSLSDGPLLIGRA
EIVYKGELRGDWRENTDHQRRRHSLSRSPSGRPRLQECR

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=281, Percent_Identity=30.9608540925267, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_METKA (Q8TY71)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613719.1
- ProteinModelPortal:   Q8TY71
- GeneID:   1477737
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0434
- NMPDR:   fig|190192.1.peg.432
- HOGENOM:   HBG399442
- OMA:   PDELSAC
- BioCyc:   MKAN190192:MK0434-MONOMER
- BRENDA:   5.1.1.7
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30994; Mature: 30994

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 73-73 ACT_SITE 196-196

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFWKVHGARNDFVLVDETEEEVVPESDKPDFARWACDRRSGVGADGVVFIRSDPPSVEM
CCEEEECCCCCCEEEEECCHHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCEEE
RIFNRDGSEAEFCGNAARCVVKYVTEVRGENVKILRTLSGAHRVEVQGGWIAVEVPEAEI
EEECCCCCHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCEEEEECCHHHH
KKVVELGYEVDAGVPHFVRLTERDPIHDFGGLTDEAKTIFSEYEPKGGVNVTYAAPSVDE
HHHHHCCCCCCCCCCCEEEECCCCCCHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCHHH
LRVRTFERGVGWTPACGSGVVAASLVYSEIFGPFEEVSVRTAGGCLRVSLSDGPLLIGRA
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHEEEECCCEEEEEECCCCEEEEEE
EIVYKGELRGDWRENTDHQRRRHSLSRSPSGRPRLQECR
EEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHCCC
>Mature Secondary Structure
MQFWKVHGARNDFVLVDETEEEVVPESDKPDFARWACDRRSGVGADGVVFIRSDPPSVEM
CCEEEECCCCCCEEEEECCHHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCEEE
RIFNRDGSEAEFCGNAARCVVKYVTEVRGENVKILRTLSGAHRVEVQGGWIAVEVPEAEI
EEECCCCCHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCEEEEECCHHHH
KKVVELGYEVDAGVPHFVRLTERDPIHDFGGLTDEAKTIFSEYEPKGGVNVTYAAPSVDE
HHHHHCCCCCCCCCCCEEEECCCCCCHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCHHH
LRVRTFERGVGWTPACGSGVVAASLVYSEIFGPFEEVSVRTAGGCLRVSLSDGPLLIGRA
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHEEEECCCEEEEEECCCCEEEEEE
EIVYKGELRGDWRENTDHQRRRHSLSRSPSGRPRLQECR
EEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014