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The map label for this gene is cimA

Identifier: 20093829

GI number: 20093829

Start: 373774

End: 375273

Strand: Direct

Name: cimA

Synonym: MK0391

Alternate gene names: 20093829

Gene position: 373774-375273 (Clockwise)

Preceding gene: 20093812

Following gene: 20093832

Centisome position: 22.05

GC content: 62.33

Gene sequence:

>1500_bases
TTGCCCGATCGGGTACGGATCTTCGACACGACGCTAAGAGACGGTGAGCAGACACCGGGCGTCAGCCTCACGGTGGAGGA
AAAGGTCGAGATCGCGAGGAAGTTGGACGAGTTCGGCGTGGATACCATCGAAGCGGGCTTCCCCGTAGCCAGTGAGGGTG
AGTTCGAGGCAGTGAGGGCGATAGCAGGCGAGGAACTGGACGCGGAGATATGCGGGTTGGCCAGGTGCGTGAAGGGGGAC
ATAGACGCGGCGATCGACGCCGACGTGGACTGCGTGCACGTGTTCATAGCCACGTCGGACATCCACCTCAGATACAAGTT
GGAGATGTCCCGGGAAGAGGCATTGGAGCGTGCCATAGAGGGCGTGGAGTATGCCAGTGACCACGGAGTCACCGTGGAGT
TCTCCGCGGAGGACGCCACACGGACGGACCGGGACTACCTACTCGAGGTCTATAAAGCTACCGTGGAAGCCGGCGCGGAT
CGTGTCAACGTCCCGGATACTGTGGGAGTTATGACCCCTCCCGAGATGTATCGACTGACGGCGGAAGTTGTCGACGCCGT
AGACGTGCCGGTCAGCGTGCACTGCCACAACGACTTCGGCATGGCCGTGGCCAACTCATTGGCCGCCGTCGAAGCCGGGG
CTGAACAGGTTCATGTGACCGTGAACGGGATCGGCGAGCGTGCCGGTAACGCCTCGCTGGAACAGGTCGTAATGGCCCTC
AAAGCGCTGTACGATATCGAGTTGGACGTGAGGACCGAGATGCTCGTCGAGCTCTCACGGCTCGTGGAGCGACTGACGGG
CGTCGTAGTACCGCCGAACACCCCGATAGTCGGCGAGAACGCCTTCGCCCACGAGTCGGGGATTCACTCCCACGGCGTGA
TCAAGAAGGCGGAGACGTACGAACCTATACGGCCCGAGGACGTCGGTCATAGACGGCGTATAGTACTAGGTAAGCACGCC
GGTCGGCACGCGATCAAGAAGAAGCTCGAGGAGATGGGGATCGAGGTCACGGAGGAACAACTCGACGAGATCGTTCGGCG
CGTCAAGGAGCTCGGGGATAAGGGCAAGCGCGTCACCGAAGACGATCTTGAAGCCATAGCCCGAGACGTCGTCGGCGAGG
TACCCGAGTCCGAAGCTGCGGTCAAGCTGGAAGAGATCGCCGTGATGACCGGGAACAAATTCACGCCGACGGCGTCGGTC
CGTGTGTACCTGGACGGTGAAGAGCACGAAGCGGCATCGACCGGTGTGGGCTCCGTTGACGCGGCGATACGGGCACTGCG
CGAAGCGATCGAGGAGCTCGGTATGGACGTGGAGCTGAAGGAGTACCGCCTAGAGGCCATCACCGGGGGCACCGACGCCC
TCGCCGAGGTCACGGTGAGGTTGGAGGACGAGGACGGGAACGTCACCACGGCCCGCGGTGCCGCCGAGGATATCGTGATG
GCCAGCGTGAAGGCGTTCGTTCGGGGCGTGAATCGGCTGGCACGTCGACGTAGGGACTAA

Upstream 100 bases:

>100_bases
GCTCCTTCAGCTTCAGTTTCAACTCCTCGGTCCTCAAGCTCGTCTCCCCTCGGGGGCTCGAAAATGGTCGAGGGGACTTC
CTTTCTGCTGGGAGCGTAGG

Downstream 100 bases:

>100_bases
CGCGTCGGGTCCTCCAACCCTAACTCTTCCGCCACTTCTCGAACGGCACGCACCGATCCTTCCGGGAGCTCGATACCCTC
CCGAAGTGCGCGTTCTCGGC

Product: (R)-citramalate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MPDRVRIFDTTLRDGEQTPGVSLTVEEKVEIARKLDEFGVDTIEAGFPVASEGEFEAVRAIAGEELDAEICGLARCVKGD
IDAAIDADVDCVHVFIATSDIHLRYKLEMSREEALERAIEGVEYASDHGVTVEFSAEDATRTDRDYLLEVYKATVEAGAD
RVNVPDTVGVMTPPEMYRLTAEVVDAVDVPVSVHCHNDFGMAVANSLAAVEAGAEQVHVTVNGIGERAGNASLEQVVMAL
KALYDIELDVRTEMLVELSRLVERLTGVVVPPNTPIVGENAFAHESGIHSHGVIKKAETYEPIRPEDVGHRRRIVLGKHA
GRHAIKKKLEEMGIEVTEEQLDEIVRRVKELGDKGKRVTEDDLEAIARDVVGEVPESEAAVKLEEIAVMTGNKFTPTASV
RVYLDGEEHEAASTGVGSVDAAIRALREAIEELGMDVELKEYRLEAITGGTDALAEVTVRLEDEDGNVTTARGAAEDIVM
ASVKAFVRGVNRLARRRRD

Sequences:

>Translated_499_residues
MPDRVRIFDTTLRDGEQTPGVSLTVEEKVEIARKLDEFGVDTIEAGFPVASEGEFEAVRAIAGEELDAEICGLARCVKGD
IDAAIDADVDCVHVFIATSDIHLRYKLEMSREEALERAIEGVEYASDHGVTVEFSAEDATRTDRDYLLEVYKATVEAGAD
RVNVPDTVGVMTPPEMYRLTAEVVDAVDVPVSVHCHNDFGMAVANSLAAVEAGAEQVHVTVNGIGERAGNASLEQVVMAL
KALYDIELDVRTEMLVELSRLVERLTGVVVPPNTPIVGENAFAHESGIHSHGVIKKAETYEPIRPEDVGHRRRIVLGKHA
GRHAIKKKLEEMGIEVTEEQLDEIVRRVKELGDKGKRVTEDDLEAIARDVVGEVPESEAAVKLEEIAVMTGNKFTPTASV
RVYLDGEEHEAASTGVGSVDAAIRALREAIEELGMDVELKEYRLEAITGGTDALAEVTVRLEDEDGNVTTARGAAEDIVM
ASVKAFVRGVNRLARRRRD
>Mature_498_residues
PDRVRIFDTTLRDGEQTPGVSLTVEEKVEIARKLDEFGVDTIEAGFPVASEGEFEAVRAIAGEELDAEICGLARCVKGDI
DAAIDADVDCVHVFIATSDIHLRYKLEMSREEALERAIEGVEYASDHGVTVEFSAEDATRTDRDYLLEVYKATVEAGADR
VNVPDTVGVMTPPEMYRLTAEVVDAVDVPVSVHCHNDFGMAVANSLAAVEAGAEQVHVTVNGIGERAGNASLEQVVMALK
ALYDIELDVRTEMLVELSRLVERLTGVVVPPNTPIVGENAFAHESGIHSHGVIKKAETYEPIRPEDVGHRRRIVLGKHAG
RHAIKKKLEEMGIEVTEEQLDEIVRRVKELGDKGKRVTEDDLEAIARDVVGEVPESEAAVKLEEIAVMTGNKFTPTASVR
VYLDGEEHEAASTGVGSVDAAIRALREAIEELGMDVELKEYRLEAITGGTDALAEVTVRLEDEDGNVTTARGAAEDIVMA
SVKAFVRGVNRLARRRRD

Specific function: Catalyzes the condensation of pyruvate and acetyl- coenzyme A to form (R)-citramalate

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family

Homologues:

Organism=Homo sapiens, GI62198232, Length=284, Percent_Identity=25, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786261, Length=508, Percent_Identity=42.1259842519685, Blast_Score=344, Evalue=6e-96,
Organism=Saccharomyces cerevisiae, GI6320019, Length=385, Percent_Identity=34.2857142857143, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6320071, Length=374, Percent_Identity=34.4919786096257, Blast_Score=220, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6324682, Length=551, Percent_Identity=24.5009074410163, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324225, Length=540, Percent_Identity=24.4444444444444, Blast_Score=120, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24582381, Length=282, Percent_Identity=27.3049645390071, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): CIMA_METKA (Q8TYB1)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613676.1
- ProteinModelPortal:   Q8TYB1
- GeneID:   1477694
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0391
- NMPDR:   fig|190192.1.peg.389
- HOGENOM:   HBG701974
- OMA:   ENSFAHE
- ProtClustDB:   PRK09389
- BioCyc:   MKAN190192:MK0391-MONOMER
- BRENDA:   2.3.1.182
- HAMAP:   MF_01028
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR02090

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.1.182

Molecular weight: Translated: 54340; Mature: 54209

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDRVRIFDTTLRDGEQTPGVSLTVEEKVEIARKLDEFGVDTIEAGFPVASEGEFEAVRA
CCCCEEEEECHHCCCCCCCCEEEEHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHH
IAGEELDAEICGLARCVKGDIDAAIDADVDCVHVFIATSDIHLRYKLEMSREEALERAIE
HHCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCEEEEEEECCCHHHHHHHHHH
GVEYASDHGVTVEFSAEDATRTDRDYLLEVYKATVEAGADRVNVPDTVGVMTPPEMYRLT
CCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
AEVVDAVDVPVSVHCHNDFGMAVANSLAAVEAGAEQVHVTVNGIGERAGNASLEQVVMAL
HHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHH
KALYDIELDVRTEMLVELSRLVERLTGVVVPPNTPIVGENAFAHESGIHSHGVIKKAETY
HHHHCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCEECCCCC
EPIRPEDVGHRRRIVLGKHAGRHAIKKKLEEMGIEVTEEQLDEIVRRVKELGDKGKRVTE
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHCCCCCCH
DDLEAIARDVVGEVPESEAAVKLEEIAVMTGNKFTPTASVRVYLDGEEHEAASTGVGSVD
HHHHHHHHHHHCCCCCCHHHEEHHHEEEECCCCCCCCCEEEEEECCCCCCHHHCCCCHHH
AAIRALREAIEELGMDVELKEYRLEAITGGTDALAEVTVRLEDEDGNVTTARGAAEDIVM
HHHHHHHHHHHHHCCCEEHHHHEEHHCCCCCCCEEEEEEEEECCCCCEEECCCCHHHHHH
ASVKAFVRGVNRLARRRRD
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PDRVRIFDTTLRDGEQTPGVSLTVEEKVEIARKLDEFGVDTIEAGFPVASEGEFEAVRA
CCCEEEEECHHCCCCCCCCEEEEHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHH
IAGEELDAEICGLARCVKGDIDAAIDADVDCVHVFIATSDIHLRYKLEMSREEALERAIE
HHCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCEEEEEEECCCHHHHHHHHHH
GVEYASDHGVTVEFSAEDATRTDRDYLLEVYKATVEAGADRVNVPDTVGVMTPPEMYRLT
CCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
AEVVDAVDVPVSVHCHNDFGMAVANSLAAVEAGAEQVHVTVNGIGERAGNASLEQVVMAL
HHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHH
KALYDIELDVRTEMLVELSRLVERLTGVVVPPNTPIVGENAFAHESGIHSHGVIKKAETY
HHHHCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCEECCCCC
EPIRPEDVGHRRRIVLGKHAGRHAIKKKLEEMGIEVTEEQLDEIVRRVKELGDKGKRVTE
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHCCCCCCH
DDLEAIARDVVGEVPESEAAVKLEEIAVMTGNKFTPTASVRVYLDGEEHEAASTGVGSVD
HHHHHHHHHHHCCCCCCHHHEEHHHEEEECCCCCCCCCEEEEEECCCCCCHHHCCCCHHH
AAIRALREAIEELGMDVELKEYRLEAITGGTDALAEVTVRLEDEDGNVTTARGAAEDIVM
HHHHHHHHHHHHHCCCEEHHHHEEHHCCCCCCCEEEEEEEEECCCCCEEECCCCHHHHHH
ASVKAFVRGVNRLARRRRD
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014