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The map label for this gene is TrpS

Identifier: 20093781

GI number: 20093781

Start: 334027

End: 335151

Strand: Direct

Name: TrpS

Synonym: MK0343

Alternate gene names: 20093781

Gene position: 334027-335151 (Clockwise)

Preceding gene: 20093780

Following gene: 20093782

Centisome position: 19.71

GC content: 58.58

Gene sequence:

>1125_bases
CTGATCGATCCCTGGGACGTAGAAGAAGTCGATTACGAACGCCTTACCGAGGAGTTCGGCATTCGACCGATCGACGAGAA
AGTCCGAGAGTTACTACCACGGCGTTTCCCACTGCTAGACCGTGGCATAGTCTTCGGGCACCGCGATTACGACTCCTTCC
TCAAAGATTACAACGACGGCAAACTCGTCAGCGTCCTCAGCGGAATGATGCCCAGCGGCCGAATGCACCTCGGCCACAAG
ACGGTCGTCGATCAACTAGTGTTTTACCAGCAGGAGATGGACGTCAAGGTGTACGTTCCCATCGCCGACCTGGAGGCACA
CCATGCCCGGAACATGGACCTAGATCGGGCACACAGGATCGCCGTCGAAGAGTACGTCCTCAACTACGCCGCGTTGGGTC
TCGACCTGGACCCGGATCGATGTGAGATCTACCTCCAATCCGAGAGGAAGACGGTCCAGCGGATGGCACTGTTGCTAGCG
GGCCGACTCACGTGGAACACCGTGAAAAACACGTACGGGTTCACCGGCGAGACCAACATGGGCCACGCTTTCGCGCCGAT
CGTCCAAGCCGCCGATATACTACACCCACAGGAGATCGAGGGACCACACCGGGTTCTCGTACCCGTAGGCGTCGATCAGG
ACCCTCACCTCCGGTTGACTCGGGATATAGCGGAAAAGGAGGATCTGATCAAGCCGGCCAGCACGTACCACAGGTTCATG
ACCGGACTGACGGGAGGCAAAATGTCCAGTAGTAAACCCAACACCGCGATATTCCTCACCGACGATCCCGAGACGGCGAA
AGAAAAGGTGTGGAACGCGAAGACCGGTGGAGGAGCGACGCTCGAGGAACATCGCGAGCACGGTGGCAACCCCGACGAGT
GCGTGGTGTACGAACTCATGGTGTACCATCTGGCCGACCGTATCGGAGGGGACGAAAAGCTACGGGAGATCCGGAAGAAG
TGTCGGGAAGGGGACATCATCTGCGGTGAGTGCAAACGGATGGTAGGAGAGGCGCTAGCGGAGATACTGGAAGAACTGGA
GCGTCGCCGTGAAGACGTTCGAGACGAGCTCCCGGACCTACTATCACAGCACCCGGACGCGCCCGAAGTACCGGAGGACT
GGTAG

Upstream 100 bases:

>100_bases
TACCACCTAGAGAAGGCCGAGGAGGAAGGCCTCATCGATTACGTGAAGGAGAAGTTCGTGGAGAGCGAGTGAGCGGGTGT
GAACTCCTATACCCGAAACC

Downstream 100 bases:

>100_bases
GATGATTCCACCGTTCCAAGCTTTAGTAACGTGTCCCGCCGGGTTCGAATTCCGTTGTGTCGAAGAGCTCGAGGATATAC
TCAAAGAGCAGGACCCGTAC

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MIDPWDVEEVDYERLTEEFGIRPIDEKVRELLPRRFPLLDRGIVFGHRDYDSFLKDYNDGKLVSVLSGMMPSGRMHLGHK
TVVDQLVFYQQEMDVKVYVPIADLEAHHARNMDLDRAHRIAVEEYVLNYAALGLDLDPDRCEIYLQSERKTVQRMALLLA
GRLTWNTVKNTYGFTGETNMGHAFAPIVQAADILHPQEIEGPHRVLVPVGVDQDPHLRLTRDIAEKEDLIKPASTYHRFM
TGLTGGKMSSSKPNTAIFLTDDPETAKEKVWNAKTGGGATLEEHREHGGNPDECVVYELMVYHLADRIGGDEKLREIRKK
CREGDIICGECKRMVGEALAEILEELERRREDVRDELPDLLSQHPDAPEVPEDW

Sequences:

>Translated_374_residues
MIDPWDVEEVDYERLTEEFGIRPIDEKVRELLPRRFPLLDRGIVFGHRDYDSFLKDYNDGKLVSVLSGMMPSGRMHLGHK
TVVDQLVFYQQEMDVKVYVPIADLEAHHARNMDLDRAHRIAVEEYVLNYAALGLDLDPDRCEIYLQSERKTVQRMALLLA
GRLTWNTVKNTYGFTGETNMGHAFAPIVQAADILHPQEIEGPHRVLVPVGVDQDPHLRLTRDIAEKEDLIKPASTYHRFM
TGLTGGKMSSSKPNTAIFLTDDPETAKEKVWNAKTGGGATLEEHREHGGNPDECVVYELMVYHLADRIGGDEKLREIRKK
CREGDIICGECKRMVGEALAEILEELERRREDVRDELPDLLSQHPDAPEVPEDW
>Mature_374_residues
MIDPWDVEEVDYERLTEEFGIRPIDEKVRELLPRRFPLLDRGIVFGHRDYDSFLKDYNDGKLVSVLSGMMPSGRMHLGHK
TVVDQLVFYQQEMDVKVYVPIADLEAHHARNMDLDRAHRIAVEEYVLNYAALGLDLDPDRCEIYLQSERKTVQRMALLLA
GRLTWNTVKNTYGFTGETNMGHAFAPIVQAADILHPQEIEGPHRVLVPVGVDQDPHLRLTRDIAEKEDLIKPASTYHRFM
TGLTGGKMSSSKPNTAIFLTDDPETAKEKVWNAKTGGGATLEEHREHGGNPDECVVYELMVYHLADRIGGDEKLREIRKK
CREGDIICGECKRMVGEALAEILEELERRREDVRDELPDLLSQHPDAPEVPEDW

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI47419920, Length=378, Percent_Identity=32.2751322751323, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI47419918, Length=378, Percent_Identity=32.2751322751323, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI47419916, Length=378, Percent_Identity=32.2751322751323, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI47419914, Length=378, Percent_Identity=32.2751322751323, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI239750058, Length=142, Percent_Identity=35.2112676056338, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI310120693, Length=142, Percent_Identity=35.2112676056338, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI239755558, Length=142, Percent_Identity=34.5070422535211, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI72001474, Length=376, Percent_Identity=31.9148936170213, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6324475, Length=391, Percent_Identity=29.4117647058824, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI17864462, Length=382, Percent_Identity=30.8900523560209, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24645295, Length=382, Percent_Identity=30.8900523560209, Blast_Score=163, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_METKA (Q8TYF7)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613628.1
- ProteinModelPortal:   Q8TYF7
- SMR:   Q8TYF7
- GeneID:   1477646
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0343
- NMPDR:   fig|190192.1.peg.341
- HOGENOM:   HBG435805
- OMA:   ADILHPQ
- ProtClustDB:   PRK12285
- BioCyc:   MKAN190192:MK0343-MONOMER
- BRENDA:   6.1.1.2
- GO:   GO:0005737
- HAMAP:   MF_00140_A
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- InterPro:   IPR020653
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 42734; Mature: 42734

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDPWDVEEVDYERLTEEFGIRPIDEKVRELLPRRFPLLDRGIVFGHRDYDSFLKDYNDG
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHCCEEECCCCHHHHHHHCCCC
KLVSVLSGMMPSGRMHLGHKTVVDQLVFYQQEMDVKVYVPIADLEAHHARNMDLDRAHRI
HHHHHHHCCCCCCCEECCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHCCCCHHHHHHH
AVEEYVLNYAALGLDLDPDRCEIYLQSERKTVQRMALLLAGRLTWNTVKNTYGFTGETNM
HHHHHHHHHHHHCCCCCCCHHEEEEHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCC
GHAFAPIVQAADILHPQEIEGPHRVLVPVGVDQDPHLRLTRDIAEKEDLIKPASTYHRFM
CHHHHHHHHHHHHCCCHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
TGLTGGKMSSSKPNTAIFLTDDPETAKEKVWNAKTGGGATLEEHREHGGNPDECVVYELM
HHCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCCCCHHHHHHCCCCCCHHHHHHHH
VYHLADRIGGDEKLREIRKKCREGDIICGECKRMVGEALAEILEELERRREDVRDELPDL
HHHHHHHCCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSQHPDAPEVPEDW
HHCCCCCCCCCCCC
>Mature Secondary Structure
MIDPWDVEEVDYERLTEEFGIRPIDEKVRELLPRRFPLLDRGIVFGHRDYDSFLKDYNDG
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHCCEEECCCCHHHHHHHCCCC
KLVSVLSGMMPSGRMHLGHKTVVDQLVFYQQEMDVKVYVPIADLEAHHARNMDLDRAHRI
HHHHHHHCCCCCCCEECCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHCCCCHHHHHHH
AVEEYVLNYAALGLDLDPDRCEIYLQSERKTVQRMALLLAGRLTWNTVKNTYGFTGETNM
HHHHHHHHHHHHCCCCCCCHHEEEEHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCC
GHAFAPIVQAADILHPQEIEGPHRVLVPVGVDQDPHLRLTRDIAEKEDLIKPASTYHRFM
CHHHHHHHHHHHHCCCHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
TGLTGGKMSSSKPNTAIFLTDDPETAKEKVWNAKTGGGATLEEHREHGGNPDECVVYELM
HHCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCCCCHHHHHHCCCCCCHHHHHHHH
VYHLADRIGGDEKLREIRKKCREGDIICGECKRMVGEALAEILEELERRREDVRDELPDL
HHHHHHHCCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSQHPDAPEVPEDW
HHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014