Click here to switch to the map view.

The map label for this gene is ThrB

Identifier: 20093771

GI number: 20093771

Start: 324530

End: 325426

Strand: Direct

Name: ThrB

Synonym: MK0333

Alternate gene names: 20093771

Gene position: 324530-325426 (Clockwise)

Preceding gene: 20093769

Following gene: 20093774

Centisome position: 19.15

GC content: 69.68

Gene sequence:

>897_bases
GTGACGCCGTTGTCGACGGTCGTACGGGCACCGGCGACGATCGCCAACGTCGGTCCGGGGTTCGACGTGTTCGGGCTGGC
CGTCGACGGGTTCCACGACGTCGTGGAGGCACACGAGGCGGACGGCGTGAGGATCGTCACCGAGGACCCGATCCCGACCG
ATCCCGAGCGTAACACGGCGGGACGTGTGGCCCTGAGGATGGTGGAGGAGTTCGACCTGCCCGGGGTGTCCTTGGAGATC
AGGAAGGGCGTCCCGATGGGGGGATTGGGTAGCAGCGCGGCCTCGGCGGTGGCGGCGGCGGTGGCTATCGACCGGGAGTT
CGAACTGGGGCTCGAGGAGTCCGAGCTGCTCAGGTTCGCGGCGGAGGGGGAGCGCGCGGCTGCGGGGGAACCCCACTACG
ACAACGTGGCCCCGTGCCTGCTGGGCGGTTTCGTGATCTGGCGGTTCGAGCGGGAGTACGTGAGGCTGGAGGTGCCGGGA
GACCTCAGGTTCGTGACGGTGACGCCGACCGGGGTGAGGGTGACCACCGAGGAGGCCAGGAAGGCCCTCAGGGAGCGGCC
GCCCTCGCTGGACGACGTGGTGAACAACCTCTCGGCGGTGGCGCTGATGGTGCACGCGCTCCACGAGGAGGACGCGGAGA
CGTTCGCGAGGATGGTCGGATGGGACAGGATCTCGGAACCCGTGCGTAAGCGGTTCGTACCCCGGTACAGGGAGCTCCGG
GAGACCGCCTACGGGACCGGAGCGCTGGGGTTCGCGATCAGCGGGGCGGGACCGACTGTCTTCGCCGTGTGCTGGCGCGA
GGACGCCGAGGACGTCAGGACGGCGCTCGAGGACGTGTTGGACGGGAAGTGCGTGTCGGCCGTGCACCGGGTCTCGGACG
GGGCGGAAGTGGCTTAG

Upstream 100 bases:

>100_bases
CGGCGAGACGCCGGTACCGGGGGCTAGAGCGAAGTAGCGGCTGACGTTCAAGGGCACCACCCGGTCCCCCGGAGGGCCGG
CGCTAAGTATCCTTGTCGGG

Downstream 100 bases:

>100_bases
CCTCTCCGTCAGCGGCCTGGAACGTCCTCACCGATCGGGCGGCCGATTAGATCATCATCCGGAGAGGGTCTTCCGACGGT
TCACACCGTCGTGGATCAGC

Product: homoserine kinase

Products: NA

Alternate protein names: HK; HSK

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTPLSTVVRAPATIANVGPGFDVFGLAVDGFHDVVEAHEADGVRIVTEDPIPTDPERNTAGRVALRMVEEFDLPGVSLEI
RKGVPMGGLGSSAASAVAAAVAIDREFELGLEESELLRFAAEGERAAAGEPHYDNVAPCLLGGFVIWRFEREYVRLEVPG
DLRFVTVTPTGVRVTTEEARKALRERPPSLDDVVNNLSAVALMVHALHEEDAETFARMVGWDRISEPVRKRFVPRYRELR
ETAYGTGALGFAISGAGPTVFAVCWREDAEDVRTALEDVLDGKCVSAVHRVSDGAEVA

Sequences:

>Translated_298_residues
MTPLSTVVRAPATIANVGPGFDVFGLAVDGFHDVVEAHEADGVRIVTEDPIPTDPERNTAGRVALRMVEEFDLPGVSLEI
RKGVPMGGLGSSAASAVAAAVAIDREFELGLEESELLRFAAEGERAAAGEPHYDNVAPCLLGGFVIWRFEREYVRLEVPG
DLRFVTVTPTGVRVTTEEARKALRERPPSLDDVVNNLSAVALMVHALHEEDAETFARMVGWDRISEPVRKRFVPRYRELR
ETAYGTGALGFAISGAGPTVFAVCWREDAEDVRTALEDVLDGKCVSAVHRVSDGAEVA
>Mature_297_residues
TPLSTVVRAPATIANVGPGFDVFGLAVDGFHDVVEAHEADGVRIVTEDPIPTDPERNTAGRVALRMVEEFDLPGVSLEIR
KGVPMGGLGSSAASAVAAAVAIDREFELGLEESELLRFAAEGERAAAGEPHYDNVAPCLLGGFVIWRFEREYVRLEVPGD
LRFVTVTPTGVRVTTEEARKALRERPPSLDDVVNNLSAVALMVHALHEEDAETFARMVGWDRISEPVRKRFVPRYRELRE
TAYGTGALGFAISGAGPTVFAVCWREDAEDVRTALEDVLDGKCVSAVHRVSDGAEVA

Specific function: Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate

COG id: COG0083

COG function: function code E; Homoserine kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. Homoserine kinase subfamily

Homologues:

Organism=Escherichia coli, GI1786184, Length=271, Percent_Identity=35.7933579335793, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KHSE_METKA (Q8TYG6)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613618.1
- ProteinModelPortal:   Q8TYG6
- SMR:   Q8TYG6
- GeneID:   1477636
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0333
- NMPDR:   fig|190192.1.peg.331
- HOGENOM:   HBG646290
- OMA:   NNALMST
- BioCyc:   MKAN190192:MK0333-MONOMER
- BRENDA:   2.7.1.39
- GO:   GO:0005737
- HAMAP:   MF_00384
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR006203
- InterPro:   IPR000870
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PIRSF:   PIRSF000676
- PRINTS:   PR00958
- TIGRFAMs:   TIGR00191

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.39

Molecular weight: Translated: 32194; Mature: 32062

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00627 GHMP_KINASES_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLSTVVRAPATIANVGPGFDVFGLAVDGFHDVVEAHEADGVRIVTEDPIPTDPERNTA
CCCHHHHHHCCHHHHCCCCCCCEEHEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHH
GRVALRMVEEFDLPGVSLEIRKGVPMGGLGSSAASAVAAAVAIDREFELGLEESELLRFA
HHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCHHHHHHHH
AEGERAAAGEPHYDNVAPCLLGGFVIWRFEREYVRLEVPGDLRFVTVTPTGVRVTTEEAR
HCCCCCCCCCCCCCCHHHHHHHHHHHEEEECCEEEEECCCCEEEEEECCCCEEEEHHHHH
KALRERPPSLDDVVNNLSAVALMVHALHEEDAETFARMVGWDRISEPVRKRFVPRYRELR
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
ETAYGTGALGFAISGAGPTVFAVCWREDAEDVRTALEDVLDGKCVSAVHRVSDGAEVA
HHHCCCCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TPLSTVVRAPATIANVGPGFDVFGLAVDGFHDVVEAHEADGVRIVTEDPIPTDPERNTA
CCHHHHHHCCHHHHCCCCCCCEEHEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHH
GRVALRMVEEFDLPGVSLEIRKGVPMGGLGSSAASAVAAAVAIDREFELGLEESELLRFA
HHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCHHHHHHHH
AEGERAAAGEPHYDNVAPCLLGGFVIWRFEREYVRLEVPGDLRFVTVTPTGVRVTTEEAR
HCCCCCCCCCCCCCCHHHHHHHHHHHEEEECCEEEEECCCCEEEEEECCCCEEEEHHHHH
KALRERPPSLDDVVNNLSAVALMVHALHEEDAETFARMVGWDRISEPVRKRFVPRYRELR
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
ETAYGTGALGFAISGAGPTVFAVCWREDAEDVRTALEDVLDGKCVSAVHRVSDGAEVA
HHHCCCCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014