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The map label for this gene is FwdB_1 [H]

Identifier: 20093699

GI number: 20093699

Start: 248342

End: 249646

Strand: Reverse

Name: FwdB_1 [H]

Synonym: MK0259

Alternate gene names: 20093699

Gene position: 249646-248342 (Counterclockwise)

Preceding gene: 20093700

Following gene: 20093697

Centisome position: 14.73

GC content: 57.55

Gene sequence:

>1305_bases
TTGGGTCGTAAAGTGATCAAGGATGTGGTCTGTCCGTTCTGTGGTACGCTGTGTGATGATCTAGAGGTGGTCGTGGAGGA
CGGTGAGATCGTGGAAGTACGGCATGCTTGTAGGATCGGTGCTGCGAAGTTCTTGACGGCGCAGGAGGATCATCGTCACA
CCGAGCCGATGATCAAAGAGAACGGAGAATGGAAGAAAATCGACTACGAAGACGCCGCAGAAGAGACGGCAAGAATACTT
GTTGAGGCGAAATGGCCTCTGATGTATGGCTGGTCTTCAACGCTATGTGAAGCACATGAAGTTGGGATAGAGATTGCAGA
AAAAGTTGGAGCTGTGATAGATAATACCGCTTCAGTGTGTCATGGACCGTCAACACTTGGATTGCAAGACGCCGGTGTTC
CTAGCTGTACATTGGGTGAAGTGAAGAATCGAGCGGACGTCATCATCTACTGGGGTTGCAACCCTATGCACGCCCACCCG
CGGCACATGAGCCGTTACACCGCCTTCACCCGCGGCTTCTTCCGACCGAAGGGTCGTGAGGATCGTACTATCATCGTCGT
GGACCCGCGGAGGACCGCTACGGCCGAGCTAGCGGACGTCCACCTGCAGGTTAGACCCAACGAGGACTATGAGCTGATCA
GCGCGCTCCGGGCGGCTGTTCACGGGATCGAGATCGAGCGCGAGGAGGTGGCGGGAATTCCGGTGGAAGCCGTGCACGAG
GTCGCTGACCTGATCAAGGAGGCCAGCTTCGGCACACTGTTCTGGGGCATGGGCATCACGATGTCCCGGGGTCGTCATCG
GAACATCGACAACGCGATCTGCTTGATCCGAGATCTGAACGAGTACGCGAAGTGGACTCTGATCATGATGCGAGGTCACT
ACAACGTGACCGGCTTCAACGAGGTGCTCACGTGGACTACGGGCTACCCGTTCGCCGTGGACTTCAGCCGTGGGTACCCG
CGGTACAACCCAGGCGAGTTCTCCGCGGTGGACGTACTGATCAGGGGAGAAGTGGACGCCGCCTTCGTAATCGCCAGTGA
TCCGGGTGCGCACTTCCCGCGGAAGGCTGTGGAGCACATGGCTAGGATCCCGCTCGTGTGCGTGGACCCGCACTGGACGC
CGACCGCCGAACTCGCCGACCTGTACGTGCCCGTAACCATCGCCGGCATCGAATGGGAAGGCACCGCCTACCGAATGGAC
TCAGTACCCATCAGGATGCGCAAGGTCGTCGAACCACCCGAAAGCATGCTCAACGACGTAGAATTCCTCGAAATGGTCAT
AGAAAAGGTCGAGGAGATGGTGTGA

Upstream 100 bases:

>100_bases
ATTGGGCTTGTTGCGCCCTTTATGAACACGAATAACCATTGAACTCCGGGATTAATAACTTTCAAAAGCTGAGACGATCA
CGGCGTTTACGGGGGTCATA

Downstream 100 bases:

>100_bases
GCGGGCCCTAGGCGATCGACTCCAAGAACGTCTCGATCCTAGTTTTCAGTTGTTCCACGTCCTCCTCGCTGTAGTCGGAT
TCAAGTTTTAGTGCAGGGAT

Product: formylmethanofuran dehydrogenase subunit B

Products: NA

Alternate protein names: Tungsten-containing formylmethanofuran dehydrogenase II subunit B [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MGRKVIKDVVCPFCGTLCDDLEVVVEDGEIVEVRHACRIGAAKFLTAQEDHRHTEPMIKENGEWKKIDYEDAAEETARIL
VEAKWPLMYGWSSTLCEAHEVGIEIAEKVGAVIDNTASVCHGPSTLGLQDAGVPSCTLGEVKNRADVIIYWGCNPMHAHP
RHMSRYTAFTRGFFRPKGREDRTIIVVDPRRTATAELADVHLQVRPNEDYELISALRAAVHGIEIEREEVAGIPVEAVHE
VADLIKEASFGTLFWGMGITMSRGRHRNIDNAICLIRDLNEYAKWTLIMMRGHYNVTGFNEVLTWTTGYPFAVDFSRGYP
RYNPGEFSAVDVLIRGEVDAAFVIASDPGAHFPRKAVEHMARIPLVCVDPHWTPTAELADLYVPVTIAGIEWEGTAYRMD
SVPIRMRKVVEPPESMLNDVEFLEMVIEKVEEMV

Sequences:

>Translated_434_residues
MGRKVIKDVVCPFCGTLCDDLEVVVEDGEIVEVRHACRIGAAKFLTAQEDHRHTEPMIKENGEWKKIDYEDAAEETARIL
VEAKWPLMYGWSSTLCEAHEVGIEIAEKVGAVIDNTASVCHGPSTLGLQDAGVPSCTLGEVKNRADVIIYWGCNPMHAHP
RHMSRYTAFTRGFFRPKGREDRTIIVVDPRRTATAELADVHLQVRPNEDYELISALRAAVHGIEIEREEVAGIPVEAVHE
VADLIKEASFGTLFWGMGITMSRGRHRNIDNAICLIRDLNEYAKWTLIMMRGHYNVTGFNEVLTWTTGYPFAVDFSRGYP
RYNPGEFSAVDVLIRGEVDAAFVIASDPGAHFPRKAVEHMARIPLVCVDPHWTPTAELADLYVPVTIAGIEWEGTAYRMD
SVPIRMRKVVEPPESMLNDVEFLEMVIEKVEEMV
>Mature_433_residues
GRKVIKDVVCPFCGTLCDDLEVVVEDGEIVEVRHACRIGAAKFLTAQEDHRHTEPMIKENGEWKKIDYEDAAEETARILV
EAKWPLMYGWSSTLCEAHEVGIEIAEKVGAVIDNTASVCHGPSTLGLQDAGVPSCTLGEVKNRADVIIYWGCNPMHAHPR
HMSRYTAFTRGFFRPKGREDRTIIVVDPRRTATAELADVHLQVRPNEDYELISALRAAVHGIEIEREEVAGIPVEAVHEV
ADLIKEASFGTLFWGMGITMSRGRHRNIDNAICLIRDLNEYAKWTLIMMRGHYNVTGFNEVLTWTTGYPFAVDFSRGYPR
YNPGEFSAVDVLIRGEVDAAFVIASDPGAHFPRKAVEHMARIPLVCVDPHWTPTAELADLYVPVTIAGIEWEGTAYRMDS
VPIRMRKVVEPPESMLNDVEFLEMVIEKVEEMV

Specific function: Catalyzes the reversible oxidation of CO(2) and methanofuran (MFR) to N-formylmethanofuran (CHO-MFR). This enzyme is oxygen-labile [H]

COG id: COG1029

COG function: function code C; Formylmethanofuran dehydrogenase subunit B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fwdB family [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=512, Percent_Identity=24.0234375, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI3868719, Length=266, Percent_Identity=25.9398496240602, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016457
- InterPro:   IPR006656 [H]

Pfam domain/function: PF00384 Molybdopterin [H]

EC number: =1.2.99.5 [H]

Molecular weight: Translated: 48717; Mature: 48586

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRKVIKDVVCPFCGTLCDDLEVVVEDGEIVEVRHACRIGAAKFLTAQEDHRHTEPMIKE
CCHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEHHHHHHCHHHHEECCHHCCCCCCHHHC
NGEWKKIDYEDAAEETARILVEAKWPLMYGWSSTLCEAHEVGIEIAEKVGAVIDNTASVC
CCCEEEECCHHHHHHHHEEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
HGPSTLGLQDAGVPSCTLGEVKNRADVIIYWGCNPMHAHPRHMSRYTAFTRGFFRPKGRE
CCCCCCCCCCCCCCCCCHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCC
DRTIIVVDPRRTATAELADVHLQVRPNEDYELISALRAAVHGIEIEREEVAGIPVEAVHE
CCEEEEECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCEEEHHHHCCCCHHHHHH
VADLIKEASFGTLFWGMGITMSRGRHRNIDNAICLIRDLNEYAKWTLIMMRGHYNVTGFN
HHHHHHHCCCCEEEEECCEEECCCCCCCCCHHEEHHHHHHHHCEEEEEEEECCCCCCCHH
EVLTWTTGYPFAVDFSRGYPRYNPGEFSAVDVLIRGEVDAAFVIASDPGAHFPRKAVEHM
HHEEEECCCCEEEECCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHH
ARIPLVCVDPHWTPTAELADLYVPVTIAGIEWEGTAYRMDSVPIRMRKVVEPPESMLNDV
HHCCEEEECCCCCCCHHHHHEEEEEEEEEEEECCCEEEECCCCHHHHHHCCCHHHHHHHH
EFLEMVIEKVEEMV
HHHHHHHHHHHHCC
>Mature Secondary Structure 
GRKVIKDVVCPFCGTLCDDLEVVVEDGEIVEVRHACRIGAAKFLTAQEDHRHTEPMIKE
CHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEHHHHHHCHHHHEECCHHCCCCCCHHHC
NGEWKKIDYEDAAEETARILVEAKWPLMYGWSSTLCEAHEVGIEIAEKVGAVIDNTASVC
CCCEEEECCHHHHHHHHEEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
HGPSTLGLQDAGVPSCTLGEVKNRADVIIYWGCNPMHAHPRHMSRYTAFTRGFFRPKGRE
CCCCCCCCCCCCCCCCCHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCC
DRTIIVVDPRRTATAELADVHLQVRPNEDYELISALRAAVHGIEIEREEVAGIPVEAVHE
CCEEEEECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCEEEHHHHCCCCHHHHHH
VADLIKEASFGTLFWGMGITMSRGRHRNIDNAICLIRDLNEYAKWTLIMMRGHYNVTGFN
HHHHHHHCCCCEEEEECCEEECCCCCCCCCHHEEHHHHHHHHCEEEEEEEECCCCCCCHH
EVLTWTTGYPFAVDFSRGYPRYNPGEFSAVDVLIRGEVDAAFVIASDPGAHFPRKAVEHM
HHEEEECCCCEEEECCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHH
ARIPLVCVDPHWTPTAELADLYVPVTIAGIEWEGTAYRMDSVPIRMRKVVEPPESMLNDV
HHCCEEEECCCCCCCHHHHHEEEEEEEEEEEECCCEEEECCCCHHHHHHCCCHHHHHHHH
EFLEMVIEKVEEMV
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]