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The map label for this gene is gatA [H]

Identifier: 20093678

GI number: 20093678

Start: 225321

End: 226688

Strand: Direct

Name: gatA [H]

Synonym: MK0238

Alternate gene names: 20093678

Gene position: 225321-226688 (Clockwise)

Preceding gene: 20093674

Following gene: 20093679

Centisome position: 13.29

GC content: 66.59

Gene sequence:

>1368_bases
ATGCTGAGGGAAGCGGTAAAAGACATCGAATCGGGTCACGTCGACCCGTCGGAGCGGATCCATGAGTATCTCGAAAAGAT
CGACCGAGAGAACCCGGATATAAATGCATTGATCTATATCAATGGGGGAGCGCCGGAGGAGGTCGGATCGGAGGGGCCGC
TCGCTGGAGTCGCCATAGCCGTCAAGGCCAACATCAACGTGGAGGGGATGCCGTGCGACTGCGCGTCCAAGACGCTGGAG
GGGTACCGGGCTCCCTTCGACGCGACGGTCGTGCGGAGGATCAAGGAAGCGGGCGCCGCGGTGATCGGTATCGCGAACAT
GGACGAGTTCGCGGCCGGCTCCTCGGGCGAGACCTCGTGCCACGGACCTACCGATAACCCGAGGTGTCCGGGGCGCATCC
CGGGCGGGTCGAGCTCGGGGAGCGCCGCGGCCGTCGCGGCGGGCCTCTGCGACGCGGCCCTGGGCTCGGACACGGGCGGG
TCGATCCGGAACCCGGCGTCACACTGCGGTGTGGTCGGGTTCAAGCCCACGTACGGCCTGGTCCCGCGGCAGGGTCTCAT
CGACCTGGCCATGAGTCTGGATCAGATCGGACCCATCACCAGGACCGTCGAGGACGCCGCGCTGCTGCTGGAGGTGATCG
CGGGCCCGGGTGAGGACGTCGAAGGGACCGTGCGGAACGCCGAGGTCCCCCGGTTCTCGGAGCTGCTGGACCCGGAGGAA
GTGGAAGGTATGCGCATAGGTCTCGTGAGGGAGTTCCTCGAGGTCTCGGAGCCCGAGATCGCGGAGGTCGCCAAGGAGGC
GGCTCGTGCCCTGGAGCGGGCCGGGGCCACCGTCGAGGAGGTGAGCCTGGGTCGGAAGCTCGTCGACGTGGCACTACCCA
CGTACTACGTGATCAACTACGTGGAGTTCTTCTCGGCGACGCGCCGGTTCGACGGACGGAGGTACGGTCGTAGGATCGAG
CACGTCTGCGGAAAGGAAGTTCTCCGGAGGATCGTGGCCGGGGCCGCGATCAGCCGGCAGGAGGTCCGCGGTCAGTACTA
CGAGCGGGCGCTGAGAGCCAGGACGTGGATCCGACGCAGACTGTTGGAGGCGTTGGAGGGCTACGACGCGTTGCTGGGAC
CGACGGTGCCGAAGCCTCCACATCGTATCGGAGAGGAGTTGAGCGTCCGTGAGATGTACGGGTACGACGTGCTGACCGTG
ATCCCTAACCTGGCCGGGTGTCCGGCCGGAAGCGTGCCGTTCGACACGATTAACGTGGATGGCGACCGGGTGCCGTGCGG
TGTTCAGGTGATCGCGCGGCCGTGGGAGGATCTGACCGCCCTGAACGTCATGGCGGCCCTCGAGCGGGCCGCCTCCTTCG
AGCCCTGA

Upstream 100 bases:

>100_bases
CCACCGTGGGCGCCGCGGAGGTGGAACCTATAAGGGTAATTAAGAGCGCGAGCGCCGGGATCCACGGACCCCTCAACCGC
CCACCCCGCGGGGAGGAACG

Downstream 100 bases:

>100_bases
GACTCCATGCTCGAGTTCTTCCCTTGACAGCTCCGACCTCTGTCCCTATTCGGGACCGGCAGTCGGACTGTTCACGCATA
GCTTATGCATGATTCACGTA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MLREAVKDIESGHVDPSERIHEYLEKIDRENPDINALIYINGGAPEEVGSEGPLAGVAIAVKANINVEGMPCDCASKTLE
GYRAPFDATVVRRIKEAGAAVIGIANMDEFAAGSSGETSCHGPTDNPRCPGRIPGGSSSGSAAAVAAGLCDAALGSDTGG
SIRNPASHCGVVGFKPTYGLVPRQGLIDLAMSLDQIGPITRTVEDAALLLEVIAGPGEDVEGTVRNAEVPRFSELLDPEE
VEGMRIGLVREFLEVSEPEIAEVAKEAARALERAGATVEEVSLGRKLVDVALPTYYVINYVEFFSATRRFDGRRYGRRIE
HVCGKEVLRRIVAGAAISRQEVRGQYYERALRARTWIRRRLLEALEGYDALLGPTVPKPPHRIGEELSVREMYGYDVLTV
IPNLAGCPAGSVPFDTINVDGDRVPCGVQVIARPWEDLTALNVMAALERAASFEP

Sequences:

>Translated_455_residues
MLREAVKDIESGHVDPSERIHEYLEKIDRENPDINALIYINGGAPEEVGSEGPLAGVAIAVKANINVEGMPCDCASKTLE
GYRAPFDATVVRRIKEAGAAVIGIANMDEFAAGSSGETSCHGPTDNPRCPGRIPGGSSSGSAAAVAAGLCDAALGSDTGG
SIRNPASHCGVVGFKPTYGLVPRQGLIDLAMSLDQIGPITRTVEDAALLLEVIAGPGEDVEGTVRNAEVPRFSELLDPEE
VEGMRIGLVREFLEVSEPEIAEVAKEAARALERAGATVEEVSLGRKLVDVALPTYYVINYVEFFSATRRFDGRRYGRRIE
HVCGKEVLRRIVAGAAISRQEVRGQYYERALRARTWIRRRLLEALEGYDALLGPTVPKPPHRIGEELSVREMYGYDVLTV
IPNLAGCPAGSVPFDTINVDGDRVPCGVQVIARPWEDLTALNVMAALERAASFEP
>Mature_455_residues
MLREAVKDIESGHVDPSERIHEYLEKIDRENPDINALIYINGGAPEEVGSEGPLAGVAIAVKANINVEGMPCDCASKTLE
GYRAPFDATVVRRIKEAGAAVIGIANMDEFAAGSSGETSCHGPTDNPRCPGRIPGGSSSGSAAAVAAGLCDAALGSDTGG
SIRNPASHCGVVGFKPTYGLVPRQGLIDLAMSLDQIGPITRTVEDAALLLEVIAGPGEDVEGTVRNAEVPRFSELLDPEE
VEGMRIGLVREFLEVSEPEIAEVAKEAARALERAGATVEEVSLGRKLVDVALPTYYVINYVEFFSATRRFDGRRYGRRIE
HVCGKEVLRRIVAGAAISRQEVRGQYYERALRARTWIRRRLLEALEGYDALLGPTVPKPPHRIGEELSVREMYGYDVLTV
IPNLAGCPAGSVPFDTINVDGDRVPCGVQVIARPWEDLTALNVMAALERAASFEP

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=510, Percent_Identity=34.9019607843137, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI195972892, Length=490, Percent_Identity=25.7142857142857, Blast_Score=118, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17543272, Length=412, Percent_Identity=34.2233009708738, Blast_Score=185, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17537465, Length=490, Percent_Identity=25.1020408163265, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=470, Percent_Identity=24.6808510638298, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=160, Percent_Identity=32.5, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=469, Percent_Identity=30.7036247334755, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6319685, Length=395, Percent_Identity=29.620253164557, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648113, Length=501, Percent_Identity=33.3333333333333, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24644968, Length=493, Percent_Identity=26.369168356998, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI161078093, Length=506, Percent_Identity=26.2845849802372, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24652985, Length=453, Percent_Identity=25.8278145695364, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI19922090, Length=453, Percent_Identity=25.8278145695364, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24652981, Length=453, Percent_Identity=25.8278145695364, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24652983, Length=453, Percent_Identity=25.8278145695364, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24648435, Length=481, Percent_Identity=24.5322245322245, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648437, Length=481, Percent_Identity=24.5322245322245, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45550774, Length=481, Percent_Identity=24.5322245322245, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21356731, Length=474, Percent_Identity=24.8945147679325, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648441, Length=417, Percent_Identity=25.4196642685851, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24648439, Length=417, Percent_Identity=25.4196642685851, Blast_Score=95, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 48830; Mature: 48830

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLREAVKDIESGHVDPSERIHEYLEKIDRENPDINALIYINGGAPEEVGSEGPLAGVAIA
CCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHCCCCCCCCEEEEE
VKANINVEGMPCDCASKTLEGYRAPFDATVVRRIKEAGAAVIGIANMDEFAAGSSGETSC
EEECCEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCC
HGPTDNPRCPGRIPGGSSSGSAAAVAAGLCDAALGSDTGGSIRNPASHCGVVGFKPTYGL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCC
VPRQGLIDLAMSLDQIGPITRTVEDAALLLEVIAGPGEDVEGTVRNAEVPRFSELLDPEE
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCHH
VEGMRIGLVREFLEVSEPEIAEVAKEAARALERAGATVEEVSLGRKLVDVALPTYYVINY
HCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
VEFFSATRRFDGRRYGRRIEHVCGKEVLRRIVAGAAISRQEVRGQYYERALRARTWIRRR
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEALEGYDALLGPTVPKPPHRIGEELSVREMYGYDVLTVIPNLAGCPAGSVPFDTINVD
HHHHHHCCHHHHCCCCCCCHHHHCCCCCHHHHHCCHHHHHHCCCCCCCCCCCCCCEEECC
GDRVPCGVQVIARPWEDLTALNVMAALERAASFEP
CCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLREAVKDIESGHVDPSERIHEYLEKIDRENPDINALIYINGGAPEEVGSEGPLAGVAIA
CCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHCCCCCCCCEEEEE
VKANINVEGMPCDCASKTLEGYRAPFDATVVRRIKEAGAAVIGIANMDEFAAGSSGETSC
EEECCEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCC
HGPTDNPRCPGRIPGGSSSGSAAAVAAGLCDAALGSDTGGSIRNPASHCGVVGFKPTYGL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCC
VPRQGLIDLAMSLDQIGPITRTVEDAALLLEVIAGPGEDVEGTVRNAEVPRFSELLDPEE
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCHH
VEGMRIGLVREFLEVSEPEIAEVAKEAARALERAGATVEEVSLGRKLVDVALPTYYVINY
HCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
VEFFSATRRFDGRRYGRRIEHVCGKEVLRRIVAGAAISRQEVRGQYYERALRARTWIRRR
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEALEGYDALLGPTVPKPPHRIGEELSVREMYGYDVLTVIPNLAGCPAGSVPFDTINVD
HHHHHHCCHHHHCCCCCCCHHHHCCCCCHHHHHCCHHHHHHCCCCCCCCCCCCCCEEECC
GDRVPCGVQVIARPWEDLTALNVMAALERAASFEP
CCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]