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The map label for this gene is Nth_1 [H]

Identifier: 20093633

GI number: 20093633

Start: 187517

End: 188218

Strand: Reverse

Name: Nth_1 [H]

Synonym: MK0193

Alternate gene names: 20093633

Gene position: 188218-187517 (Counterclockwise)

Preceding gene: 20093634

Following gene: 20093632

Centisome position: 11.1

GC content: 57.41

Gene sequence:

>702_bases
ATGTTCCCCTCGGTACCGAGTTTAAGGATTTCGCCGGGAGTAGGGCCGGGGAGACGCGTGAATCCCGTGATGGAGATCGT
AAAGCACTACGAGGATCACCCCATCATGAAAAAGCTGAAGGTACGGCGTGATCCTTTCCGGGCCCTCATTCAAGCCATCA
TATCTCAACGGACCAGGGACGACGTGACGGACAGAGTGGCGGAAAGGTTCCTGCGGAAATTCAAGACTCCGAAAGACGTC
GCAGAAGTGAATCTGAAGGATCTCGTCGAGACGCTCCGCGACGCCGGGCTCTACCGTCAGAAGGCCAAAATGATCAAGGA
GTGCTGTGAACGTATTCTCGCGGACGGATTGGATCTCGAGGAGATCGTTCAGAAGCCTACGGAAGAAGCGCGACGGGAGT
TAATGCGTCTGCCAGGGGTCGGTCCGAAGACAGCCGACGTGGTGTTGCTGTTCGCGGGTGGTCATGACGTCTGTCCGGTC
GATACCCACGTAGCTCGGGTTTCGAGGAGGCTCGGTCTGACCGACTCGAAGGAGTACTTCGAGGTCCAGGAGGCTGTACA
CGAGATGGTTCCCGAGGGCGAACGCGGTAAAGCGCACCTCGCACTCATACAATTCGGACGCGAGATATGCCGTCCTCGAA
AACCCCAGTGCGAGTTGTGCTTCGTCCGTCGTTTCTGCCCGTACGGAGGGGAACAGGCTTGA

Upstream 100 bases:

>100_bases
CGGGGAATTGAACCCCGCGCCCCACTCGGGGCGCGCCGGATCTGAAGTCCGGCCCGGGGACCACCCCGGTGGCGGCGGCC
TTTCGGTACCGGGCTTCAGG

Downstream 100 bases:

>100_bases
CTGTCAGGGAAGTGTATGAGGAACTGCGCGAGGTAGTCCTGGAGAAGCTCGGTAAAGGCGAGGATCCCCTCCCTCAGATT
CTCGGGCAGAGAGAGGTCAA

Product: EndoIII-related endonuclease

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MFPSVPSLRISPGVGPGRRVNPVMEIVKHYEDHPIMKKLKVRRDPFRALIQAIISQRTRDDVTDRVAERFLRKFKTPKDV
AEVNLKDLVETLRDAGLYRQKAKMIKECCERILADGLDLEEIVQKPTEEARRELMRLPGVGPKTADVVLLFAGGHDVCPV
DTHVARVSRRLGLTDSKEYFEVQEAVHEMVPEGERGKAHLALIQFGREICRPRKPQCELCFVRRFCPYGGEQA

Sequences:

>Translated_233_residues
MFPSVPSLRISPGVGPGRRVNPVMEIVKHYEDHPIMKKLKVRRDPFRALIQAIISQRTRDDVTDRVAERFLRKFKTPKDV
AEVNLKDLVETLRDAGLYRQKAKMIKECCERILADGLDLEEIVQKPTEEARRELMRLPGVGPKTADVVLLFAGGHDVCPV
DTHVARVSRRLGLTDSKEYFEVQEAVHEMVPEGERGKAHLALIQFGREICRPRKPQCELCFVRRFCPYGGEQA
>Mature_233_residues
MFPSVPSLRISPGVGPGRRVNPVMEIVKHYEDHPIMKKLKVRRDPFRALIQAIISQRTRDDVTDRVAERFLRKFKTPKDV
AEVNLKDLVETLRDAGLYRQKAKMIKECCERILADGLDLEEIVQKPTEEARRELMRLPGVGPKTADVVLLFAGGHDVCPV
DTHVARVSRRLGLTDSKEYFEVQEAVHEMVPEGERGKAHLALIQFGREICRPRKPQCELCFVRRFCPYGGEQA

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=193, Percent_Identity=31.0880829015544, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1787920, Length=185, Percent_Identity=32.972972972973, Blast_Score=94, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17554540, Length=183, Percent_Identity=31.1475409836066, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45550361, Length=189, Percent_Identity=31.2169312169312, Blast_Score=88, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 26610; Mature: 26610

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPSVPSLRISPGVGPGRRVNPVMEIVKHYEDHPIMKKLKVRRDPFRALIQAIISQRTRD
CCCCCCCCEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
DVTDRVAERFLRKFKTPKDVAEVNLKDLVETLRDAGLYRQKAKMIKECCERILADGLDLE
HHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EIVQKPTEEARRELMRLPGVGPKTADVVLLFAGGHDVCPVDTHVARVSRRLGLTDSKEYF
HHHHCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHH
EVQEAVHEMVPEGERGKAHLALIQFGREICRPRKPQCELCFVRRFCPYGGEQA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFPSVPSLRISPGVGPGRRVNPVMEIVKHYEDHPIMKKLKVRRDPFRALIQAIISQRTRD
CCCCCCCCEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
DVTDRVAERFLRKFKTPKDVAEVNLKDLVETLRDAGLYRQKAKMIKECCERILADGLDLE
HHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EIVQKPTEEARRELMRLPGVGPKTADVVLLFAGGHDVCPVDTHVARVSRRLGLTDSKEYF
HHHHCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHH
EVQEAVHEMVPEGERGKAHLALIQFGREICRPRKPQCELCFVRRFCPYGGEQA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]