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The map label for this gene is AroE

Identifier: 20093557

GI number: 20093557

Start: 112165

End: 113037

Strand: Reverse

Name: AroE

Synonym: MK0117

Alternate gene names: 20093557

Gene position: 113037-112165 (Counterclockwise)

Preceding gene: 20093558

Following gene: 20093556

Centisome position: 6.67

GC content: 63.46

Gene sequence:

>873_bases
GTGAGCGTGTCCGTATCGGTCGACGCTGAAACCAACGTCGTGGGACTCATCGGACATCCCGTCGAGCACTCCCTGTCTCC
GGCCATGCACAACGCCGCGTTCAAGGAGCTCGGACTGAACTACGTGTACCTGGCCTTCGACGTGCCACCGGAGAGGCTCG
AGGGCGCCGTCCGAGGTGCCGCCGATCTGGGGATCGTCGGCCTTAACGTGACGATACCTCACAAAGAAGCGGTCATGGAG
CTGTGCGATGAGCTAGATCGCGACGCCGAACTGATCGGGGCCGTGAACACCGTCAGGTTCTCACGGGGTAAAATCGAGGG
ATTCAACACCGACGGCGAGGGGTTCCTGCGGGCGCTGCGGGAAGAGACCTACTTCGATCCTCGAGGTACCAAGTCCGTGA
TCCTCGGTGCAGGAGGCGCGGCCCGCGCCGTCTCCTTCAAGCTCGCCACGGAAGGGGCCGACGAGATCGTCATCGCCAAC
CGTACGGTAGACCGGGCAGAGAGGCTCGCGGAGGAGCTGAAGGAGAAAGTGGGGGTAAAGGCACGCGCCATAGGGCTGGA
CGGGGACGAAATCGAGAGGGAGCTTCGGGACGCCGACCTGCTGGTTGACGCCACGCCGGTCGGTATGTACCCGAACGAGG
ACGAACCACCCCTCGTTACCGCTGACCAAATGCACGAGGACTTGATCGTGAACGACCTCGTGTACAACCCACCGAGGACG
CGGCTGCTCGAGGAGGCCGAGAAGGCGGGAGCCACCCCGGTGAGCGGTGTTGGCATGTTAGTGTACCAGGGGGCACTGGC
GTTCGAGCTGTGGACGGGGGAAGAGGCACCGGTGGAGGTCATGAGGGAAGCCGTCCTGGAACACCTCCGATGA

Upstream 100 bases:

>100_bases
CGTGATCCGCTCAACGTCGGCAACGTCTGCATACTGAAAGCCTCCGGGGGAACCCTCCGGGCGAAGCTGTTCAACCTACG
CAAGGCGCGGGTGATCGGCC

Downstream 100 bases:

>100_bases
ATCGTTGCGAATTTAAGGGCCAGGTCGGGTGGCTCCTGCGGGGTGTTCCGGGTGGAAATCAACGGCGTGGAGATCGAGGA
CACCTTTGCCGAGGCTTTCG

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSVSVSVDAETNVVGLIGHPVEHSLSPAMHNAAFKELGLNYVYLAFDVPPERLEGAVRGAADLGIVGLNVTIPHKEAVME
LCDELDRDAELIGAVNTVRFSRGKIEGFNTDGEGFLRALREETYFDPRGTKSVILGAGGAARAVSFKLATEGADEIVIAN
RTVDRAERLAEELKEKVGVKARAIGLDGDEIERELRDADLLVDATPVGMYPNEDEPPLVTADQMHEDLIVNDLVYNPPRT
RLLEEAEKAGATPVSGVGMLVYQGALAFELWTGEEAPVEVMREAVLEHLR

Sequences:

>Translated_290_residues
MSVSVSVDAETNVVGLIGHPVEHSLSPAMHNAAFKELGLNYVYLAFDVPPERLEGAVRGAADLGIVGLNVTIPHKEAVME
LCDELDRDAELIGAVNTVRFSRGKIEGFNTDGEGFLRALREETYFDPRGTKSVILGAGGAARAVSFKLATEGADEIVIAN
RTVDRAERLAEELKEKVGVKARAIGLDGDEIERELRDADLLVDATPVGMYPNEDEPPLVTADQMHEDLIVNDLVYNPPRT
RLLEEAEKAGATPVSGVGMLVYQGALAFELWTGEEAPVEVMREAVLEHLR
>Mature_289_residues
SVSVSVDAETNVVGLIGHPVEHSLSPAMHNAAFKELGLNYVYLAFDVPPERLEGAVRGAADLGIVGLNVTIPHKEAVMEL
CDELDRDAELIGAVNTVRFSRGKIEGFNTDGEGFLRALREETYFDPRGTKSVILGAGGAARAVSFKLATEGADEIVIANR
TVDRAERLAEELKEKVGVKARAIGLDGDEIERELRDADLLVDATPVGMYPNEDEPPLVTADQMHEDLIVNDLVYNPPRTR
LLEEAEKAGATPVSGVGMLVYQGALAFELWTGEEAPVEVMREAVLEHLR

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1787983, Length=285, Percent_Identity=38.2456140350877, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1789675, Length=276, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6320332, Length=288, Percent_Identity=31.9444444444444, Blast_Score=106, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_METKA (Q8TZ24)

Other databases:

- EMBL:   AE009439
- PIR:   S65949
- RefSeq:   NP_613404.1
- ProteinModelPortal:   Q8TZ24
- SMR:   Q8TZ24
- GeneID:   1477420
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0117
- NMPDR:   fig|190192.1.peg.117
- HOGENOM:   HBG553408
- OMA:   GYNTDAS
- BioCyc:   MKAN190192:MK0117-MONOMER
- BRENDA:   1.1.1.25
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 31413; Mature: 31282

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: NA

Important sites: ACT_SITE 75-75

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSVSVDAETNVVGLIGHPVEHSLSPAMHNAAFKELGLNYVYLAFDVPPERLEGAVRGA
CEEEEEECCCCCEEEEECCCHHHCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCC
ADLGIVGLNVTIPHKEAVMELCDELDRDAELIGAVNTVRFSRGKIEGFNTDGEGFLRALR
CCCEEEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHH
EETYFDPRGTKSVILGAGGAARAVSFKLATEGADEIVIANRTVDRAERLAEELKEKVGVK
HHHCCCCCCCCEEEEECCCCHHEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHCCE
ARAIGLDGDEIERELRDADLLVDATPVGMYPNEDEPPLVTADQMHEDLIVNDLVYNPPRT
EEEECCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCHH
RLLEEAEKAGATPVSGVGMLVYQGALAFELWTGEEAPVEVMREAVLEHLR
HHHHHHHHCCCCCCCCCHHHEECCEEEEEEECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVSVSVDAETNVVGLIGHPVEHSLSPAMHNAAFKELGLNYVYLAFDVPPERLEGAVRGA
EEEEEECCCCCEEEEECCCHHHCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCC
ADLGIVGLNVTIPHKEAVMELCDELDRDAELIGAVNTVRFSRGKIEGFNTDGEGFLRALR
CCCEEEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHH
EETYFDPRGTKSVILGAGGAARAVSFKLATEGADEIVIANRTVDRAERLAEELKEKVGVK
HHHCCCCCCCCEEEEECCCCHHEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHCCE
ARAIGLDGDEIERELRDADLLVDATPVGMYPNEDEPPLVTADQMHEDLIVNDLVYNPPRT
EEEECCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCHH
RLLEEAEKAGATPVSGVGMLVYQGALAFELWTGEEAPVEVMREAVLEHLR
HHHHHHHHCCCCCCCCCHHHEECCEEEEEEECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014