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The map label for this gene is ThiC

Identifier: 20093546

GI number: 20093546

Start: 99868

End: 101157

Strand: Reverse

Name: ThiC

Synonym: MK0106

Alternate gene names: 20093546

Gene position: 101157-99868 (Counterclockwise)

Preceding gene: 20093547

Following gene: 20093545

Centisome position: 5.97

GC content: 65.89

Gene sequence:

>1290_bases
GTGACCCTGATGGAAGAGTGTAGGCGGACGGTACCCGATACGGTCCGGAAGATCGCGGAGGAGGAAGGCGTCCGACCGGA
GAAGCTCGCGAGACGAGTGGCCGAAGGACGGGTGGTCGTCCCGGCCCACGCGGACCGTCGGGACGAGGTCCGGCCCGTCG
GCATAGGTGAAGGGCTCCGCGTGAAAATCAACGCCAACGTCGGAACATCCCCGGAGGTCTGCGATCCCGACCTCGAAGTG
GAGAAGGCCCGTGCAGCGGTCGATCACGGTGCCGACACGGTGATGGATCTCAGCACGGGTGGAGACCTGAGAGAGATCCG
TCGACGGATCATGAAGGCCGTGGACGTGCCCGTAGGGACCGTGCCCGTGTACGAAGCGGCCGTGGAAATGACCAGACGGG
GACGGGCCGTCGTGGACATGGATGAGGACGACATGCTGCGGGCCATCGAGCGACACATGGAGGACGGTGTTGACTTCATG
ACCGTACACTGCGCGGTCACGCTGGACGCGCTCGAGGACGTCCTCCGGAGGGGTAGAGCGCTCGGGATCGTTTCGCGCGG
CGGTGCCATCGTTGCGGCCTGGATGATCCATCACGACGCCGAGAACCCCCTGTACGAGAACTTCGACTACATCCTGGAGC
TCGCGCGGGAGCACGACGTGACCCTATCCCTAGGCGACGCGATGCGTCCCGGATCCGTGCTCGACGCCAACGACGCCGCC
CAACACCGGGAGCTCGTTGTCCAGGGGGAGCTCGTGGACCGGTGCCGCGAGGCCGGTGTGCAGGCTATGGTGGAGGGACC
CGGCCACGTGCCGCTCGACCAGATACCGGCGGTGGTGAGACTGCAGAAGCGCGTGTGTGACGGGGCTCCGTTCTACGTGC
TCGGTCCCGTACCGACCGACGTGGCTCCGGGGTACGACCATATCGCCGCGGCCATCGGCGGGGCCATCGCCGCGTACCAC
GGTGCCGACTTCCTGTGTTACGTGACCCCGGCCGAGCACCTGGCGCTCCCGGACGTCAAGGACGTAATCCTCGGCGTGAT
CGCGACACGCATTGCGGCTCACGCCGCCGACACGGCCAGGGGCATGAAGTACGCCCGCCGAGAGAACGAGGAGATGGCCG
AAGCACGGTGGAACCTGGACTGGGACCGTCAGTTCGAGCTCGCCATCGACCCGAAGAAGCCACGACACTACCGGGAGGAA
CGCCCGCCGCAGGCCAAGGAGCTGTGCTCGATGTGCGGGGAGTATTGCGCTATCAAAATACTGAAAGACGCACTGGAGGA
GCGTCGGTAA

Upstream 100 bases:

>100_bases
ACTACCTACCGCGACCGTTGGTCCTCGAAATGAACGCGTTGTTCTCACAACTCATGAACTTGGCTAAGACCATGGCAGCG
CGGGCAGGGGCAGGTACGCC

Downstream 100 bases:

>100_bases
TCCGAACCCGGGAACCGTCACGATGCGGGAGGAAGCACCGCCGAAAATCCCCGAGGGCCTCCGGATCACCGCGACGCGGA
CCATGGCCGCCGCCCGTCCT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTLMEECRRTVPDTVRKIAEEEGVRPEKLARRVAEGRVVVPAHADRRDEVRPVGIGEGLRVKINANVGTSPEVCDPDLEV
EKARAAVDHGADTVMDLSTGGDLREIRRRIMKAVDVPVGTVPVYEAAVEMTRRGRAVVDMDEDDMLRAIERHMEDGVDFM
TVHCAVTLDALEDVLRRGRALGIVSRGGAIVAAWMIHHDAENPLYENFDYILELAREHDVTLSLGDAMRPGSVLDANDAA
QHRELVVQGELVDRCREAGVQAMVEGPGHVPLDQIPAVVRLQKRVCDGAPFYVLGPVPTDVAPGYDHIAAAIGGAIAAYH
GADFLCYVTPAEHLALPDVKDVILGVIATRIAAHAADTARGMKYARRENEEMAEARWNLDWDRQFELAIDPKKPRHYREE
RPPQAKELCSMCGEYCAIKILKDALEERR

Sequences:

>Translated_429_residues
MTLMEECRRTVPDTVRKIAEEEGVRPEKLARRVAEGRVVVPAHADRRDEVRPVGIGEGLRVKINANVGTSPEVCDPDLEV
EKARAAVDHGADTVMDLSTGGDLREIRRRIMKAVDVPVGTVPVYEAAVEMTRRGRAVVDMDEDDMLRAIERHMEDGVDFM
TVHCAVTLDALEDVLRRGRALGIVSRGGAIVAAWMIHHDAENPLYENFDYILELAREHDVTLSLGDAMRPGSVLDANDAA
QHRELVVQGELVDRCREAGVQAMVEGPGHVPLDQIPAVVRLQKRVCDGAPFYVLGPVPTDVAPGYDHIAAAIGGAIAAYH
GADFLCYVTPAEHLALPDVKDVILGVIATRIAAHAADTARGMKYARRENEEMAEARWNLDWDRQFELAIDPKKPRHYREE
RPPQAKELCSMCGEYCAIKILKDALEERR
>Mature_428_residues
TLMEECRRTVPDTVRKIAEEEGVRPEKLARRVAEGRVVVPAHADRRDEVRPVGIGEGLRVKINANVGTSPEVCDPDLEVE
KARAAVDHGADTVMDLSTGGDLREIRRRIMKAVDVPVGTVPVYEAAVEMTRRGRAVVDMDEDDMLRAIERHMEDGVDFMT
VHCAVTLDALEDVLRRGRALGIVSRGGAIVAAWMIHHDAENPLYENFDYILELAREHDVTLSLGDAMRPGSVLDANDAAQ
HRELVVQGELVDRCREAGVQAMVEGPGHVPLDQIPAVVRLQKRVCDGAPFYVLGPVPTDVAPGYDHIAAAIGGAIAAYHG
ADFLCYVTPAEHLALPDVKDVILGVIATRIAAHAADTARGMKYARRENEEMAEARWNLDWDRQFELAIDPKKPRHYREER
PPQAKELCSMCGEYCAIKILKDALEERR

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=407, Percent_Identity=42.997542997543, Blast_Score=326, Evalue=2e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_METKA (Q8TZ33)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613393.1
- ProteinModelPortal:   Q8TZ33
- GeneID:   1477409
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0106
- NMPDR:   fig|190192.1.peg.106
- HOGENOM:   HBG285234
- OMA:   VDFMTIH
- ProtClustDB:   PRK13352
- BioCyc:   MKAN190192:MK0106-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 47443; Mature: 47312

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: BINDING 66-66 BINDING 95-95 BINDING 124-124 BINDING 163-163 BINDING 265-265 BINDING 292-292

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLMEECRRTVPDTVRKIAEEEGVRPEKLARRVAEGRVVVPAHADRRDEVRPVGIGEGLR
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEECCCCCEE
VKINANVGTSPEVCDPDLEVEKARAAVDHGADTVMDLSTGGDLREIRRRIMKAVDVPVGT
EEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCC
VPVYEAAVEMTRRGRAVVDMDEDDMLRAIERHMEDGVDFMTVHCAVTLDALEDVLRRGRA
CHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCE
LGIVSRGGAIVAAWMIHHDAENPLYENFDYILELAREHDVTLSLGDAMRPGSVLDANDAA
EEEEECCCCEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHH
QHRELVVQGELVDRCREAGVQAMVEGPGHVPLDQIPAVVRLQKRVCDGAPFYVLGPVPTD
HHHHEEEEHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCC
VAPGYDHIAAAIGGAIAAYHGADFLCYVTPAEHLALPDVKDVILGVIATRIAAHAADTAR
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GMKYARRENEEMAEARWNLDWDRQFELAIDPKKPRHYREERPPQAKELCSMCGEYCAIKI
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHH
LKDALEERR
HHHHHHHCC
>Mature Secondary Structure 
TLMEECRRTVPDTVRKIAEEEGVRPEKLARRVAEGRVVVPAHADRRDEVRPVGIGEGLR
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEECCCCCEE
VKINANVGTSPEVCDPDLEVEKARAAVDHGADTVMDLSTGGDLREIRRRIMKAVDVPVGT
EEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCC
VPVYEAAVEMTRRGRAVVDMDEDDMLRAIERHMEDGVDFMTVHCAVTLDALEDVLRRGRA
CHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCE
LGIVSRGGAIVAAWMIHHDAENPLYENFDYILELAREHDVTLSLGDAMRPGSVLDANDAA
EEEEECCCCEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHH
QHRELVVQGELVDRCREAGVQAMVEGPGHVPLDQIPAVVRLQKRVCDGAPFYVLGPVPTD
HHHHEEEEHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCC
VAPGYDHIAAAIGGAIAAYHGADFLCYVTPAEHLALPDVKDVILGVIATRIAAHAADTAR
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GMKYARRENEEMAEARWNLDWDRQFELAIDPKKPRHYREERPPQAKELCSMCGEYCAIKI
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHH
LKDALEERR
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014