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The map label for this gene is AsnB [C]

Identifier: 20093543

GI number: 20093543

Start: 94354

End: 95916

Strand: Reverse

Name: AsnB [C]

Synonym: MK0103

Alternate gene names: 20093543

Gene position: 95916-94354 (Counterclockwise)

Preceding gene: 20093545

Following gene: 20093536

Centisome position: 5.66

GC content: 65.9

Gene sequence:

>1563_bases
GTGTGCGGTATCGCGTGTGCGGTCGGTGACGACGATATCGCCGCGATGGTCACCGCGATGGAACACCGGGGGCCCGACGG
GCGGGGCTTCGCCTCGGTCTCGGACGACGGTGTGGAGTTCTCCGAGGAGCCGCCCTCGGAGGGTGAGGTGGTCCTAGGCC
ACGTCCGGCTGTGGGTGCGCGGCGAGGCTTCAGCGGTCCAACCGATCGTCGAGGAGGATCGGGCGGTGGCCGTGAACGGG
GAGATCTACAACTACCGGAGGTTCGTCGAGGACGCGCCGTCGGACTCCTGGGCGGTGTTCGAGGTCGTAAGATCCGTACG
TGACGCGGCGGCGGCACTTCGGGTACTCCGGGGCGAGTACGCGTTCGTAGCGGCCTTCAGGGACGGCACGGTGGTCGCGG
CCAGGGACCCCATCGGCGTGCGTCCGCTGTACTACTGCGTGGAGGCCGGGCTCGCCGTGGCCTCCGAGCGCAAGGCCCTG
TGGGCGGCCGGGTTCCGCGACGTACGTAGGGTACCACCGGGAGCGCTGCTCGTGCTCCGTGACGGTCGTGTCGAGCTGAG
GAACGTGGTCGACGTCCCCAGGTCGAGACCGGGGAGAAGCTCCTGGAAAGACCTGCTAGAGGTCCTTCAGCGATCCGTTC
GGGAGCGTGTGGAGGAGACGGAGCGCGTGGGTGTGGTACTTTCGGGCGGGGTGGACAGTTCGACGGTGGCGAAACTGGCG
TCCGAGTACGTGGACGTTAAGTGCTACGCCGCGGGGTTCGAGGGTTCGGACGACGTGGAGGTCGCCGAACGGTTGTGCGA
CGAGATGGGATGGCCGTTCGTCTCGGTATCCCTCGAGGATGAGTTCGAGCGGTACGTAGTCGCCACGGTCTACGCCGTCG
AAACCTGGAACCCGATGAAGGTGGAGGTGGGGATCCCGATCCTGGCCTGCGCCGGGGCGATGTCCGACGACGGGATCCGG
GTGATGCTCTCGGGTCAGGGGGCGGACGAGCTGCTCGGTGGTTACCACCGGCACCTGCGACATTACGGTGACTGGGATCG
GTTCTCGTGGGAACTCTGGAAGGACGTGGCCGCGATACACGCCGTGAACCTGGAGCGGGACGATAAGGCCGGCATGCACC
ATTCGGTGGAGCTGCGGGTTCCATACCTGGACCTGGACGTTGTGAGGACGGGTCTGGGTATCGACCCCAGGGAGAACGTT
TCGGGGCCCGAGGACAATCTGCGCAAGCGCGCCCTGAGGCGCGTCGCGGCGGAGCTCGGACTCCCGGACTTCGTGGTGGA
GAGGCGGAAGCGGGCTACTCAGTACGGGTCGTTGACCTCCAAGATGTTGGACAAGCTCGTGCGGGAACTGGGTATCAAGC
GGGCGGTGGCTAAGCGTCTCGGATACCGGAGCCACAAGGAGCTGTTCCTGCGACTGGTGGGCAAATACCTCGGGTTCCCG
TGGGAGGCGCCGTCCGTTGAGGAAGTGGAGAGGGAGTGCGCTAGATTGGGTGTAGAGCCGGAGATCTCGGAGTTCCTGGA
GGAACGGGTGCTCACCTTCGATAGTGATCTAAGGGCCGGGTGA

Upstream 100 bases:

>100_bases
GGATCAAAACGAACGCTAAGACCACCATAGGAGCCAAAGTCCACCCCCCGCCGAACTACCACCGTGGATTTACGTGGAAT
AAGGGTTACGGGGTGATAAC

Downstream 100 bases:

>100_bases
CGAGCCTGGAGGCGTCGATCCCTTCCTCGAGGGCCGTTTCCAGGTCGATTACGTACGTTCTCTCGCGCTCCTCCATCAGG
TCGGGCGGGAAGAGGACGGC

Product: asparagine synthase (glutamine-hydrolyzing)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MCGIACAVGDDDIAAMVTAMEHRGPDGRGFASVSDDGVEFSEEPPSEGEVVLGHVRLWVRGEASAVQPIVEEDRAVAVNG
EIYNYRRFVEDAPSDSWAVFEVVRSVRDAAAALRVLRGEYAFVAAFRDGTVVAARDPIGVRPLYYCVEAGLAVASERKAL
WAAGFRDVRRVPPGALLVLRDGRVELRNVVDVPRSRPGRSSWKDLLEVLQRSVRERVEETERVGVVLSGGVDSSTVAKLA
SEYVDVKCYAAGFEGSDDVEVAERLCDEMGWPFVSVSLEDEFERYVVATVYAVETWNPMKVEVGIPILACAGAMSDDGIR
VMLSGQGADELLGGYHRHLRHYGDWDRFSWELWKDVAAIHAVNLERDDKAGMHHSVELRVPYLDLDVVRTGLGIDPRENV
SGPEDNLRKRALRRVAAELGLPDFVVERRKRATQYGSLTSKMLDKLVRELGIKRAVAKRLGYRSHKELFLRLVGKYLGFP
WEAPSVEEVERECARLGVEPEISEFLEERVLTFDSDLRAG

Sequences:

>Translated_520_residues
MCGIACAVGDDDIAAMVTAMEHRGPDGRGFASVSDDGVEFSEEPPSEGEVVLGHVRLWVRGEASAVQPIVEEDRAVAVNG
EIYNYRRFVEDAPSDSWAVFEVVRSVRDAAAALRVLRGEYAFVAAFRDGTVVAARDPIGVRPLYYCVEAGLAVASERKAL
WAAGFRDVRRVPPGALLVLRDGRVELRNVVDVPRSRPGRSSWKDLLEVLQRSVRERVEETERVGVVLSGGVDSSTVAKLA
SEYVDVKCYAAGFEGSDDVEVAERLCDEMGWPFVSVSLEDEFERYVVATVYAVETWNPMKVEVGIPILACAGAMSDDGIR
VMLSGQGADELLGGYHRHLRHYGDWDRFSWELWKDVAAIHAVNLERDDKAGMHHSVELRVPYLDLDVVRTGLGIDPRENV
SGPEDNLRKRALRRVAAELGLPDFVVERRKRATQYGSLTSKMLDKLVRELGIKRAVAKRLGYRSHKELFLRLVGKYLGFP
WEAPSVEEVERECARLGVEPEISEFLEERVLTFDSDLRAG
>Mature_520_residues
MCGIACAVGDDDIAAMVTAMEHRGPDGRGFASVSDDGVEFSEEPPSEGEVVLGHVRLWVRGEASAVQPIVEEDRAVAVNG
EIYNYRRFVEDAPSDSWAVFEVVRSVRDAAAALRVLRGEYAFVAAFRDGTVVAARDPIGVRPLYYCVEAGLAVASERKAL
WAAGFRDVRRVPPGALLVLRDGRVELRNVVDVPRSRPGRSSWKDLLEVLQRSVRERVEETERVGVVLSGGVDSSTVAKLA
SEYVDVKCYAAGFEGSDDVEVAERLCDEMGWPFVSVSLEDEFERYVVATVYAVETWNPMKVEVGIPILACAGAMSDDGIR
VMLSGQGADELLGGYHRHLRHYGDWDRFSWELWKDVAAIHAVNLERDDKAGMHHSVELRVPYLDLDVVRTGLGIDPRENV
SGPEDNLRKRALRRVAAELGLPDFVVERRKRATQYGSLTSKMLDKLVRELGIKRAVAKRLGYRSHKELFLRLVGKYLGFP
WEAPSVEEVERECARLGVEPEISEFLEERVLTFDSDLRAG

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=493, Percent_Identity=26.5720081135903, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI168229252, Length=493, Percent_Identity=26.5720081135903, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI168229250, Length=493, Percent_Identity=26.5720081135903, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI296010848, Length=470, Percent_Identity=25.9574468085106, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI296010852, Length=367, Percent_Identity=27.2479564032698, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI296010850, Length=367, Percent_Identity=27.2479564032698, Blast_Score=84, Evalue=5e-16,
Organism=Escherichia coli, GI1786889, Length=448, Percent_Identity=26.5625, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788651, Length=253, Percent_Identity=28.4584980237154, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17560178, Length=480, Percent_Identity=29.1666666666667, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI25147557, Length=412, Percent_Identity=28.8834951456311, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71993933, Length=402, Percent_Identity=29.3532338308458, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25147560, Length=292, Percent_Identity=28.4246575342466, Blast_Score=81, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325403, Length=488, Percent_Identity=23.7704918032787, Blast_Score=123, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6321563, Length=484, Percent_Identity=25, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6323542, Length=459, Percent_Identity=26.797385620915, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI45553209, Length=445, Percent_Identity=29.8876404494382, Blast_Score=144, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 57806; Mature: 57806

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIACAVGDDDIAAMVTAMEHRGPDGRGFASVSDDGVEFSEEPPSEGEVVLGHVRLWVR
CCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEE
GEASAVQPIVEEDRAVAVNGEIYNYRRFVEDAPSDSWAVFEVVRSVRDAAAALRVLRGEY
CCHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCE
AFVAAFRDGTVVAARDPIGVRPLYYCVEAGLAVASERKALWAAGFRDVRRVPPGALLVLR
EEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
DGRVELRNVVDVPRSRPGRSSWKDLLEVLQRSVRERVEETERVGVVLSGGVDSSTVAKLA
CCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHH
SEYVDVKCYAAGFEGSDDVEVAERLCDEMGWPFVSVSLEDEFERYVVATVYAVETWNPMK
HHHHCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHEEEEEEEEEECCCCEE
VEVGIPILACAGAMSDDGIRVMLSGQGADELLGGYHRHLRHYGDWDRFSWELWKDVAAIH
EEECCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AVNLERDDKAGMHHSVELRVPYLDLDVVRTGLGIDPRENVSGPEDNLRKRALRRVAAELG
HCCCCCCCCCCCCEEEEEEECEEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
LPDFVVERRKRATQYGSLTSKMLDKLVRELGIKRAVAKRLGYRSHKELFLRLVGKYLGFP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
WEAPSVEEVERECARLGVEPEISEFLEERVLTFDSDLRAG
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MCGIACAVGDDDIAAMVTAMEHRGPDGRGFASVSDDGVEFSEEPPSEGEVVLGHVRLWVR
CCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEE
GEASAVQPIVEEDRAVAVNGEIYNYRRFVEDAPSDSWAVFEVVRSVRDAAAALRVLRGEY
CCHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCE
AFVAAFRDGTVVAARDPIGVRPLYYCVEAGLAVASERKALWAAGFRDVRRVPPGALLVLR
EEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
DGRVELRNVVDVPRSRPGRSSWKDLLEVLQRSVRERVEETERVGVVLSGGVDSSTVAKLA
CCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHH
SEYVDVKCYAAGFEGSDDVEVAERLCDEMGWPFVSVSLEDEFERYVVATVYAVETWNPMK
HHHHCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHEEEEEEEEEECCCCEE
VEVGIPILACAGAMSDDGIRVMLSGQGADELLGGYHRHLRHYGDWDRFSWELWKDVAAIH
EEECCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AVNLERDDKAGMHHSVELRVPYLDLDVVRTGLGIDPRENVSGPEDNLRKRALRRVAAELG
HCCCCCCCCCCCCEEEEEEECEEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
LPDFVVERRKRATQYGSLTSKMLDKLVRELGIKRAVAKRLGYRSHKELFLRLVGKYLGFP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
WEAPSVEEVERECARLGVEPEISEFLEERVLTFDSDLRAG
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]