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The map label for this gene is pelA

Identifier: 20093478

GI number: 20093478

Start: 31721

End: 32782

Strand: Direct

Name: pelA

Synonym: MK0038

Alternate gene names: 20093478

Gene position: 31721-32782 (Clockwise)

Preceding gene: 20093477

Following gene: 20093480

Centisome position: 1.87

GC content: 55.84

Gene sequence:

>1062_bases
ATGAAGGTGGTGGAAAAGGATCTGGATAAAGGATACATAGAAGTACTTCCTGAAACGTTGGACGATCTGTGGCACTTATA
CCATGTTGTCAGGAAGGGCGATCTAGTATTCGCACTGGAAAGACGACGGGTTAAAGATGAGAGGGCTGAAACTATTAGGA
GAGATAAAGGAGAACGCAAACCGGTTTACCTAGGAGTCCGAGTAGAAGATGTAGAGTTTGACAAATACGCGAACAGATTG
CGAATCAAAGGTGTAATAGAGCACGGTCCCGAGTCTGGATCGCACCATACCGTTAATGTTACGACTGGGAAACGAATAAA
GATTGTAAAGGACGAATGGGAACGAAAAGACCTAGAGCGCATCGAAGAAGCTGAGATGTCGCGACCACCCGTGATGCTCG
TGGCCGTGGACACAGGTGAGGGGACCATCGGAATAGTCCGAGACTACGGGTTAGACGTCGTGGCTCGGGTTCGGCATAAC
GTACCGGGGAAGCGTGGCGGCGACCGGAGGGCGGAGATGCGGAAGTTCTTCCATCGACTCGCCGACGAGATCGAGCGCAT
CGCCGAGGAGGAAGGAGTGGAACACATCGTCGTGGGCGGCCCGGGGTTCGTGAAGTCGGATTTCGCAGAGTTCTTACGGG
AGGAGCGGGATATCCCAGCCCACGTGGAGGATACTGGTTCGGCGGGCGAGGCCGGGCTCATCGAGATGATCCGACGCGGA
GCGGTAGAGAGGGCCGTCGAAGAGTCTAGGGTCGCGGAGGAAGTAAAGCATCTCGAGGAAGTGTTCAAGAGGATCGGTAA
GGGTGATGATAAGGTAGCGTACGGCGTCCGAGAGTGTCTCAAAGCCGCGGAGTTCGGAGCTATCGACGTACTGCTGGTGG
CGGACGAGAAGTTCCGGGAAGCGATGGTGGAAGGTGAAGAGGACGTCCTGAACGCCGTCAAGTACGCGGAGCGTACAGGG
GCCGAGGTGCTCATCGTAAGCACGGAACACGAGTGGGGCGAGAGGCTACGGGAATTGGGGGGAATCGCCGCACTGCTTCG
GTTCTCTATCCCCACGGGGTAG

Upstream 100 bases:

>100_bases
CGGGAGTTCTTAGACTCAGCGGAGCGCGAGGCCAGGGAGCTAGGCCGGGAGTACCTACTAGTTTTCAAAGAGATATTACG
CGAGCGGGGGTGGGATAGTA

Downstream 100 bases:

>100_bases
CACGGTAATCGTGCACCGGCGAGAGCACACGTCCCCCGTGTCATGGAGTATCTCGACGTCGTGACCGGCCAGCCGGCACA
GTTCATCTATGAGGTTGACC

Product: RNA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKVVEKDLDKGYIEVLPETLDDLWHLYHVVRKGDLVFALERRRVKDERAETIRRDKGERKPVYLGVRVEDVEFDKYANRL
RIKGVIEHGPESGSHHTVNVTTGKRIKIVKDEWERKDLERIEEAEMSRPPVMLVAVDTGEGTIGIVRDYGLDVVARVRHN
VPGKRGGDRRAEMRKFFHRLADEIERIAEEEGVEHIVVGGPGFVKSDFAEFLREERDIPAHVEDTGSAGEAGLIEMIRRG
AVERAVEESRVAEEVKHLEEVFKRIGKGDDKVAYGVRECLKAAEFGAIDVLLVADEKFREAMVEGEEDVLNAVKYAERTG
AEVLIVSTEHEWGERLRELGGIAALLRFSIPTG

Sequences:

>Translated_353_residues
MKVVEKDLDKGYIEVLPETLDDLWHLYHVVRKGDLVFALERRRVKDERAETIRRDKGERKPVYLGVRVEDVEFDKYANRL
RIKGVIEHGPESGSHHTVNVTTGKRIKIVKDEWERKDLERIEEAEMSRPPVMLVAVDTGEGTIGIVRDYGLDVVARVRHN
VPGKRGGDRRAEMRKFFHRLADEIERIAEEEGVEHIVVGGPGFVKSDFAEFLREERDIPAHVEDTGSAGEAGLIEMIRRG
AVERAVEESRVAEEVKHLEEVFKRIGKGDDKVAYGVRECLKAAEFGAIDVLLVADEKFREAMVEGEEDVLNAVKYAERTG
AEVLIVSTEHEWGERLRELGGIAALLRFSIPTG
>Mature_353_residues
MKVVEKDLDKGYIEVLPETLDDLWHLYHVVRKGDLVFALERRRVKDERAETIRRDKGERKPVYLGVRVEDVEFDKYANRL
RIKGVIEHGPESGSHHTVNVTTGKRIKIVKDEWERKDLERIEEAEMSRPPVMLVAVDTGEGTIGIVRDYGLDVVARVRHN
VPGKRGGDRRAEMRKFFHRLADEIERIAEEEGVEHIVVGGPGFVKSDFAEFLREERDIPAHVEDTGSAGEAGLIEMIRRG
AVERAVEESRVAEEVKHLEEVFKRIGKGDDKVAYGVRECLKAAEFGAIDVLLVADEKFREAMVEGEEDVLNAVKYAERTG
AEVLIVSTEHEWGERLRELGGIAALLRFSIPTG

Specific function: May function in recognizing stalled ribosomes, interact with stem-loop structures in stalled mRNA molecules, and effect endonucleolytic cleavage of the mRNA. May play a role in the release non-functional ribosomes and degradation of damaged mRNAs. Has end

COG id: COG1537

COG function: function code R; Predicted RNA-binding proteins

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic release factor 1 family. Pelota subfamily

Homologues:

Organism=Homo sapiens, GI31880783, Length=373, Percent_Identity=29.4906166219839, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17554648, Length=360, Percent_Identity=26.3888888888889, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324327, Length=372, Percent_Identity=24.4623655913978, Blast_Score=102, Evalue=9e-23,
Organism=Drosophila melanogaster, GI17136914, Length=373, Percent_Identity=29.7587131367292, Blast_Score=155, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PELO_METKA (Q8TZ98)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613325.1
- HSSP:   Q9BRX2
- ProteinModelPortal:   Q8TZ98
- SMR:   Q8TZ98
- GeneID:   1477341
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0038
- NMPDR:   fig|190192.1.peg.38
- HOGENOM:   HBG646058
- OMA:   DKEDLFT
- BioCyc:   MKAN190192:MK0038-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01853
- InterPro:   IPR005140
- InterPro:   IPR005141
- InterPro:   IPR005142
- InterPro:   IPR004405
- PANTHER:   PTHR10853
- TIGRFAMs:   TIGR00111

Pfam domain/function: PF03463 eRF1_1; PF03464 eRF1_2; PF03465 eRF1_3

EC number: NA

Molecular weight: Translated: 40008; Mature: 40008

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVEKDLDKGYIEVLPETLDDLWHLYHVVRKGDLVFALERRRVKDERAETIRRDKGERK
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCCC
PVYLGVRVEDVEFDKYANRLRIKGVIEHGPESGSHHTVNVTTGKRIKIVKDEWERKDLER
CEEEEEEEECCCHHHHHHHEEEEEHHHCCCCCCCCEEEEECCCCEEEEEHHHHHHHHHHH
IEEAEMSRPPVMLVAVDTGEGTIGIVRDYGLDVVARVRHNVPGKRGGDRRAEMRKFFHRL
HHHHHCCCCCEEEEEEECCCCCEEEEHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
ADEIERIAEEEGVEHIVVGGPGFVKSDFAEFLREERDIPAHVEDTGSAGEAGLIEMIRRG
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
AVERAVEESRVAEEVKHLEEVFKRIGKGDDKVAYGVRECLKAAEFGAIDVLLVADEKFRE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHH
AMVEGEEDVLNAVKYAERTGAEVLIVSTEHEWGERLRELGGIAALLRFSIPTG
HHHCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKVVEKDLDKGYIEVLPETLDDLWHLYHVVRKGDLVFALERRRVKDERAETIRRDKGERK
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCCC
PVYLGVRVEDVEFDKYANRLRIKGVIEHGPESGSHHTVNVTTGKRIKIVKDEWERKDLER
CEEEEEEEECCCHHHHHHHEEEEEHHHCCCCCCCCEEEEECCCCEEEEEHHHHHHHHHHH
IEEAEMSRPPVMLVAVDTGEGTIGIVRDYGLDVVARVRHNVPGKRGGDRRAEMRKFFHRL
HHHHHCCCCCEEEEEEECCCCCEEEEHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
ADEIERIAEEEGVEHIVVGGPGFVKSDFAEFLREERDIPAHVEDTGSAGEAGLIEMIRRG
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
AVERAVEESRVAEEVKHLEEVFKRIGKGDDKVAYGVRECLKAAEFGAIDVLLVADEKFRE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHH
AMVEGEEDVLNAVKYAERTGAEVLIVSTEHEWGERLRELGGIAALLRFSIPTG
HHHCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014