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The map label for this gene is Pnp [C]

Identifier: 20093461

GI number: 20093461

Start: 20108

End: 20878

Strand: Reverse

Name: Pnp [C]

Synonym: MK0021

Alternate gene names: 20093461

Gene position: 20878-20108 (Counterclockwise)

Preceding gene: 20093462

Following gene: 20093452

Centisome position: 1.23

GC content: 59.92

Gene sequence:

>771_bases
ATGACCGAGGTAGGAGTGATAGGCGGCACGGGTTTCCAACCCGGTCTGCCCGAACGCAGGCGGACGGTGTTCACACCCTA
CGGCACTGTACGCGTGGACATCACCCGGGTCGGAGATCATCGAGTGTACTTCATAAACCGGCACGGTAAGGGCCACGATC
TGCCCCCACACCGTATCAACTATCGAGCCATCGTGTGGGCGATGCGGGAGCTCGGCGTGAAACGGATATTGGCGACGAAC
TCCGTCGGCGTGATCAACTCCGACGAATACGAGCCTGGAGACATCGTCCTTCCGGTGGATTTCCTCGACTTCACTAAGCG
CAGGCCGACGACGTTCTACGACGAGAAGGTCGTTCACGTCGATGTCACCGAACCCTATTGTCCGGAGCTACGTGAAGCGC
TACTGAAGGCCGCGGACGACCTAGGGTACACGGTCAAAGAAGGCGCTGTATACGTGGCCACCGAAGGACCCAGGTTCGAG
ACGCCGGCCGAGATCCGTGCCTTCCGGAAGCTCGGCGGTGATATCGTTGGAATGACAGGATTCCCGGAGGTCGTGCTCGC
CAGGGAGCTCGAGATCTGCTACGCCTCCGTGTGCTTGTGCACCAACTATGCCGCTGGCATCGACGATCGCCGCCGCACGA
TCGATGAGGTGTTCGAGCTGGTCGAGGAGCTCAGACCGAAGGCGGTGGAACTAATTGAGCGTTGCATCGAGTACATTCCA
CCGAAACGATCGTGCCCCTGCTCCCAAGCTTTGGAGGGCGCCGAGGTATGA

Upstream 100 bases:

>100_bases
CCTTGTACGATCTCGTCCTCGACACCTCCCGATGGTCCGAAGACGAGACGTTCTCCATTCTCAAAGCGGCGATAGATCCG
CTGCTGGAGCGTGAGGATCC

Downstream 100 bases:

>100_bases
GGTTGAGGGCTCTGGACCTCATTCCTGACGACTTTAAGGTCCGCCCGTTGGCACTCCGGTATCTCAAGAGATTGGAAGAC
GATTACCCGGACATATTAGA

Product: purine nucleoside phosphorylase

Products: ribose-1-phosphate; xanthine [C]

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTEVGVIGGTGFQPGLPERRRTVFTPYGTVRVDITRVGDHRVYFINRHGKGHDLPPHRINYRAIVWAMRELGVKRILATN
SVGVINSDEYEPGDIVLPVDFLDFTKRRPTTFYDEKVVHVDVTEPYCPELREALLKAADDLGYTVKEGAVYVATEGPRFE
TPAEIRAFRKLGGDIVGMTGFPEVVLARELEICYASVCLCTNYAAGIDDRRRTIDEVFELVEELRPKAVELIERCIEYIP
PKRSCPCSQALEGAEV

Sequences:

>Translated_256_residues
MTEVGVIGGTGFQPGLPERRRTVFTPYGTVRVDITRVGDHRVYFINRHGKGHDLPPHRINYRAIVWAMRELGVKRILATN
SVGVINSDEYEPGDIVLPVDFLDFTKRRPTTFYDEKVVHVDVTEPYCPELREALLKAADDLGYTVKEGAVYVATEGPRFE
TPAEIRAFRKLGGDIVGMTGFPEVVLARELEICYASVCLCTNYAAGIDDRRRTIDEVFELVEELRPKAVELIERCIEYIP
PKRSCPCSQALEGAEV
>Mature_255_residues
TEVGVIGGTGFQPGLPERRRTVFTPYGTVRVDITRVGDHRVYFINRHGKGHDLPPHRINYRAIVWAMRELGVKRILATNS
VGVINSDEYEPGDIVLPVDFLDFTKRRPTTFYDEKVVHVDVTEPYCPELREALLKAADDLGYTVKEGAVYVATEGPRFET
PAEIRAFRKLGGDIVGMTGFPEVVLARELEICYASVCLCTNYAAGIDDRRRTIDEVFELVEELRPKAVELIERCIEYIPP
KRSCPCSQALEGAEV

Specific function: The Nucleoside Phosphorylases Catalyze The Phosphorolytic Breakdown Of The N-Glycosidic Bond In The Nucleoside Molecule, With The Formation Of The Corresponding Free Bases And Pentose-1-Phosphate. This Protein Can Degrade All Purine Nucleosides Except Ade

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI47132622, Length=263, Percent_Identity=34.9809885931559, Blast_Score=160, Evalue=1e-39,
Organism=Escherichia coli, GI1788746, Length=171, Percent_Identity=29.2397660818713, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71980569, Length=242, Percent_Identity=32.6446280991736, Blast_Score=124, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6323045, Length=233, Percent_Identity=32.618025751073, Blast_Score=109, Evalue=5e-25,
Organism=Drosophila melanogaster, GI20130079, Length=273, Percent_Identity=34.4322344322344, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI221459247, Length=212, Percent_Identity=39.622641509434, Blast_Score=146, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010044
- InterPro:   IPR000845
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 28790; Mature: 28659

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVGVIGGTGFQPGLPERRRTVFTPYGTVRVDITRVGDHRVYFINRHGKGHDLPPHRIN
CCCEEEECCCCCCCCCCHHHCEEECCCCEEEEEEEEECCEEEEEEECCCCCCCCCCCCCC
YRAIVWAMRELGVKRILATNSVGVINSDEYEPGDIVLPVDFLDFTKRRPTTFYDEKVVHV
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCEECCCEEEEE
DVTEPYCPELREALLKAADDLGYTVKEGAVYVATEGPRFETPAEIRAFRKLGGDIVGMTG
ECCCCCCHHHHHHHHHHHHHCCEEEECCEEEEEECCCCCCCCHHHHHHHHHCCCEEECCC
FPEVVLARELEICYASVCLCTNYAAGIDDRRRTIDEVFELVEELRPKAVELIERCIEYIP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC
PKRSCPCSQALEGAEV
CCCCCCHHHHCCCCCC
>Mature Secondary Structure 
TEVGVIGGTGFQPGLPERRRTVFTPYGTVRVDITRVGDHRVYFINRHGKGHDLPPHRIN
CCEEEECCCCCCCCCCHHHCEEECCCCEEEEEEEEECCEEEEEEECCCCCCCCCCCCCC
YRAIVWAMRELGVKRILATNSVGVINSDEYEPGDIVLPVDFLDFTKRRPTTFYDEKVVHV
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCEECCCEEEEE
DVTEPYCPELREALLKAADDLGYTVKEGAVYVATEGPRFETPAEIRAFRKLGGDIVGMTG
ECCCCCCHHHHHHHHHHHHHCCEEEECCEEEEEECCCCCCCCHHHHHHHHHCCCEEECCC
FPEVVLARELEICYASVCLCTNYAAGIDDRRRTIDEVFELVEELRPKAVELIERCIEYIP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC
PKRSCPCSQALEGAEV
CCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: xanthosine; phosphate [C]

Specific reaction: xanthosine + phosphate = ribose-1-phosphate + xanthine [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]