Click here to switch to the map view.

The map label for this gene is RCL1

Identifier: 20093441

GI number: 20093441

Start: 748

End: 1806

Strand: Reverse

Name: RCL1

Synonym: MK0001

Alternate gene names: 20093441

Gene position: 1806-748 (Counterclockwise)

Preceding gene: 20093442

Following gene: NA

Centisome position: 0.11

GC content: 62.32

Gene sequence:

>1059_bases
ATGAGCTTGATAGAGATCGACGGTTCCTACGGTGAGGGTGGAGGCCAGATACTCCGAACGGCGGTCGGGATGTCAGCGCT
GACAGGTGAACCCGTCCGGATATACAACATCCGGGCCAACAGGCCACGTCCCGGCCTCTCACACCAGCACCTACACGCAG
TGAAGGCCGTGGCCGAGATCTGCGATGCGGAGTGCGAGGGACTCGAGATAGGTTCGACCGAGATCGTGTTCGAGCCGGGG
AAGGTGAAAGGAGGGGAGTACGAGGTAGATATCGGTACCGCGGGTAGCGTGACCCTACTGCTTCAAGCCGTCAAGCTCGC
GGCGATAGCCGCCGACGGGCCCGTGGAGATGGAAGTGCGAGGCGGGACGGACGTCAAGTGGTCACCGCCCGTGGACTACG
AGATCAACGTCAACGCACACTACCTAGACCGCCTCGGTTACCGGTACGAGCTCGAGGTCCTGCGACGCGGCCATTATCCT
CGGGGTGGAGGGATAGTCAGGGCCAGGATGGAACCCCCGAAGCGGCTGAAGCCGCTGGAAGCCGTGAAGTTCGGCGAGCT
GGAGAGCGTTCGAGGGATCTCCCACTGCGTGCGGTTACCACCCCACGTGGCGGAGCGTCAGGCCAAGGCAGCTTCGGAAA
TTATCGAGAGGGAGCTCGGGATCAGACCTGAAATCGAGATCGAGACGTACCCTAAAGGCCGCGATCCACACCTAGGTCCC
GGCAGCGGTATCGTCTTATGGGCGGAAGACGATCAAGGGAACAGGATCGGTGCCGATGCGCTGGGTGAGAAAGGCAAGCC
CGCGGAGGTGGTGGGACGAGAGGCCGCGGAGCAGCTCGTGCAGCGTCTTCGCACTGGAATGGCCCTTGACGAGCACATGG
GAGATCAGATCCTGCCATTCCTAGCGATCGCCGACGGTGAATCGGTCTTCGGTGTAACCGGTGTCGATCCGCACCTCCCG
ACGAACGCGTGGGTGGTGGAGAAATTCCTCCCCGTGAGCGTCGAAATCCGCGGGAAAGAGGGGGAGCCCGCGACAGTCGA
AGTTCGGCCCGAGGGTTGA

Upstream 100 bases:

>100_bases
ACCGATGGAGGAAGAGTGACGGCGTTAAGATCCGGCTCAGCCCCGGGTTCTCTCCTCACGAGCGCGGCCATCCGGACCCT
CCTCATCGCCGGTGCCGGTG

Downstream 100 bases:

>100_bases
GCGATGTTCACATCCGACGTAGACGTGTACGCGGAACTGCGCAAAACGGCTCCCCACTACTCCAAACAGATTTTTCTGCG
CTTCGGCGTGAACTGTTTCG

Product: RNA 3'-terminal-phosphate cyclase

Products: NA

Alternate protein names: RNA cyclase; RNA-3'-phosphate cyclase

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MSLIEIDGSYGEGGGQILRTAVGMSALTGEPVRIYNIRANRPRPGLSHQHLHAVKAVAEICDAECEGLEIGSTEIVFEPG
KVKGGEYEVDIGTAGSVTLLLQAVKLAAIAADGPVEMEVRGGTDVKWSPPVDYEINVNAHYLDRLGYRYELEVLRRGHYP
RGGGIVRARMEPPKRLKPLEAVKFGELESVRGISHCVRLPPHVAERQAKAASEIIERELGIRPEIEIETYPKGRDPHLGP
GSGIVLWAEDDQGNRIGADALGEKGKPAEVVGREAAEQLVQRLRTGMALDEHMGDQILPFLAIADGESVFGVTGVDPHLP
TNAWVVEKFLPVSVEIRGKEGEPATVEVRPEG

Sequences:

>Translated_352_residues
MSLIEIDGSYGEGGGQILRTAVGMSALTGEPVRIYNIRANRPRPGLSHQHLHAVKAVAEICDAECEGLEIGSTEIVFEPG
KVKGGEYEVDIGTAGSVTLLLQAVKLAAIAADGPVEMEVRGGTDVKWSPPVDYEINVNAHYLDRLGYRYELEVLRRGHYP
RGGGIVRARMEPPKRLKPLEAVKFGELESVRGISHCVRLPPHVAERQAKAASEIIERELGIRPEIEIETYPKGRDPHLGP
GSGIVLWAEDDQGNRIGADALGEKGKPAEVVGREAAEQLVQRLRTGMALDEHMGDQILPFLAIADGESVFGVTGVDPHLP
TNAWVVEKFLPVSVEIRGKEGEPATVEVRPEG
>Mature_351_residues
SLIEIDGSYGEGGGQILRTAVGMSALTGEPVRIYNIRANRPRPGLSHQHLHAVKAVAEICDAECEGLEIGSTEIVFEPGK
VKGGEYEVDIGTAGSVTLLLQAVKLAAIAADGPVEMEVRGGTDVKWSPPVDYEINVNAHYLDRLGYRYELEVLRRGHYPR
GGGIVRARMEPPKRLKPLEAVKFGELESVRGISHCVRLPPHVAERQAKAASEIIERELGIRPEIEIETYPKGRDPHLGPG
SGIVLWAEDDQGNRIGADALGEKGKPAEVVGREAAEQLVQRLRTGMALDEHMGDQILPFLAIADGESVFGVTGVDPHLPT
NAWVVEKFLPVSVEIRGKEGEPATVEVRPEG

Specific function: Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps:(A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA- N3'PP5'A;

COG id: COG0430

COG function: function code A; RNA 3'-terminal phosphate cyclase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4506589, Length=341, Percent_Identity=37.8299120234604, Blast_Score=232, Evalue=5e-61,
Organism=Homo sapiens, GI195927044, Length=354, Percent_Identity=36.7231638418079, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI157426877, Length=308, Percent_Identity=28.2467532467532, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI48994937, Length=339, Percent_Identity=38.9380530973451, Blast_Score=213, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI32564710, Length=291, Percent_Identity=26.8041237113402, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI18543269, Length=351, Percent_Identity=34.7578347578348, Blast_Score=208, Evalue=3e-54,
Organism=Drosophila melanogaster, GI18860065, Length=306, Percent_Identity=27.4509803921569, Blast_Score=99, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RTCA_METKA (Q8TZC9)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613288.1
- ProteinModelPortal:   Q8TZC9
- SMR:   Q8TZC9
- GeneID:   1477327
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0001
- NMPDR:   fig|190192.1.peg.1
- HOGENOM:   HBG683539
- OMA:   EIDGSYG
- ProtClustDB:   PRK04204
- BioCyc:   MKAN190192:MK0001-MONOMER
- BRENDA:   6.5.1.4
- GO:   GO:0005737
- GO:   GO:0005654
- HAMAP:   MF_00200
- InterPro:   IPR013791
- InterPro:   IPR000228
- InterPro:   IPR020719
- InterPro:   IPR013796
- InterPro:   IPR020723
- InterPro:   IPR017770
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.20
- PANTHER:   PTHR11096
- TIGRFAMs:   TIGR03399

Pfam domain/function: PF01137 RTC; PF05189 RTC_insert; SSF52913 RNA3'-term_phos_cycl_insert; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =6.5.1.4

Molecular weight: Translated: 38093; Mature: 37962

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS01287 RTC

Important sites: ACT_SITE 318-318 BINDING 102-102

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIEIDGSYGEGGGQILRTAVGMSALTGEPVRIYNIRANRPRPGLSHQHLHAVKAVAEI
CEEEEECCCCCCCHHHHHHHHHCHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
CDAECEGLEIGSTEIVFEPGKVKGGEYEVDIGTAGSVTLLLQAVKLAAIAADGPVEMEVR
HCCCCCCEEECCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHEECCCCEEEEEC
GGTDVKWSPPVDYEINVNAHYLDRLGYRYELEVLRRGHYPRGGGIVRARMEPPKRLKPLE
CCCCCEECCCCCEEEECCHHHHHHCCCEEEEHHHHCCCCCCCCCEEEEECCCHHHCCCHH
AVKFGELESVRGISHCVRLPPHVAERQAKAASEIIERELGIRPEIEIETYPKGRDPHLGP
HHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
GSGIVLWAEDDQGNRIGADALGEKGKPAEVVGREAAEQLVQRLRTGMALDEHMGDQILPF
CCCEEEEEECCCCCEECHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHEEE
LAIADGESVFGVTGVDPHLPTNAWVVEKFLPVSVEIRGKEGEPATVEVRPEG
EEEECCCCEEEECCCCCCCCCCCEEEEEECCEEEEEECCCCCCCEEEEECCC
>Mature Secondary Structure 
SLIEIDGSYGEGGGQILRTAVGMSALTGEPVRIYNIRANRPRPGLSHQHLHAVKAVAEI
EEEEECCCCCCCHHHHHHHHHCHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
CDAECEGLEIGSTEIVFEPGKVKGGEYEVDIGTAGSVTLLLQAVKLAAIAADGPVEMEVR
HCCCCCCEEECCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHEECCCCEEEEEC
GGTDVKWSPPVDYEINVNAHYLDRLGYRYELEVLRRGHYPRGGGIVRARMEPPKRLKPLE
CCCCCEECCCCCEEEECCHHHHHHCCCEEEEHHHHCCCCCCCCCEEEEECCCHHHCCCHH
AVKFGELESVRGISHCVRLPPHVAERQAKAASEIIERELGIRPEIEIETYPKGRDPHLGP
HHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
GSGIVLWAEDDQGNRIGADALGEKGKPAEVVGREAAEQLVQRLRTGMALDEHMGDQILPF
CCCEEEEEECCCCCEECHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHEEE
LAIADGESVFGVTGVDPHLPTNAWVVEKFLPVSVEIRGKEGEPATVEVRPEG
EEEECCCCEEEECCCCCCCCCCCEEEEEECCEEEEEECCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014