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The map label for this gene is gidA [H]

Identifier: 19747019

GI number: 19747019

Start: 1860692

End: 1862590

Strand: Reverse

Name: gidA [H]

Synonym: spyM18_2220

Alternate gene names: 19747019

Gene position: 1862590-1860692 (Counterclockwise)

Preceding gene: 19747020

Following gene: 19747018

Centisome position: 98.29

GC content: 39.86

Gene sequence:

>1899_bases
ATGACACATGAATTTACAGAAAGCTATGACGTCATTGTCATAGGTGCAGGTCATGCTGGTGTTGAAGCCAGTTTGGCCAC
TAGTCGTATGGGCTGTAAAACTTTATTGGCAACAATCAACCTTGATATGCTTGCTTTTATGCCATGTAATCCTTCCATTG
GAGGATCTGCCAAAGGGATAGTGGTACGAGAAATCGATGCTCTTGGCGGCGAAATGAGTAAAAATATTGATAAAACCTAT
ATCCAGATGAAAATGCTTAATACAGGTAAGGGACCAGCTGTACGCGCTCTCCGAGCTCAAGCAGATAAATCCCTGTATGC
GCGTGAAATGAAACATACGGTTGAAAAACAGGCTAATTTGACTCTTCGCCAAACGATGATTGATGATATTCTAGTTGAAG
ATGGACGAGTTGTAGGTGTTTTAACAGCCACAGGACAAAAATTTGCAGCTAAGGCAGTTGTAGTGACAACAGGAACAGCT
CTGCGTGGCGAAATTATTTTAGGAGAACTCAAATATTCTTCTGGGCCTAATAATAGTTTGGCATCAGTAACCTTAGCAGA
TAATTTAAAAAAACTCGGCTTAGAAATTGGCAGATTCAAAACAGGAACGCCTCCTCGTGTCAAAGCATCTTCTATTAATT
ACGATCAAACGGAAATTCAGCCAGGTGATGACAAACCTAATCATTTTTCATTTATGTCAAAAGATGCTGATTATCTCAAA
GATCAAATTCCCTGCTGGTTGACTTATACGAATCAAACTAGTCATGATATCATTAATCAGAACCTTTACAGAGCACCGAT
GTTTTCTGGCATTGTTAAAGGTGTAGGCCCTCGTTACTGCCCATCTATTGAAGATAAGATTGTTCGCTTTGCTGATAAAG
AACGTCATCAACTTTTCTTAGAGCCAGAGGGGCGTGATACAGAAGAAGTGTATGTTCAAGGGTTGTCAACGAGTCTTCCA
GAAGATGTCCAAAAAGATTTGATTCATTCGATCAAAGGACTTGAAAAGGCTGAAATGATGCGCACTGGTTATGCTATTGA
ATATGATATTGTTTTACCTCACCAGCTACGTGCCACACTTGAAACCAAGCTAATTTCAGGACTCTTTACGGCTGGGCAAA
CTAATGGAACTTCTGGGTATGAAGAGGCTGCTGGCCAAGGACTTATTGCTGGAATCAATGCTGCTTTGAAAGTTCAAGGT
AAGCCAGAACTTATTTTGAAACGGAGCGATGCTTATATTGGCGTTATGATTGACGATTTGGTTACCAAAGGAACCTTGGA
ACCTTATCGATTGTTGACATCGCGAGCTGAGTATCGTTTGATTTTACGTCATGACAACGCTGATATGCGTTTGACAGAGA
TTGGTCGCGACATTGGGTTGGTTGATGATGAGCGTTGGAAGGCCTTCGAAATTAAGAAAAACCAATTTGATAACGAATTA
AAACGTCTGAACAGTATCAAATTAAAACCAGTAAAAGCAACCAACGATCGTGTGCAGGAACTTGGTTTTAAACCATTGAC
GGATGCTATGACAGCTAAAGAATTTATGCGTCGCCCAGAAATTGATTATGCGACTGCAGTCTCTTTTGTTGGTCCTGCGG
CTGAAGATTTAGATGCTAAAATCATTGAACTTTTAGAAACAGAAATCAAGTACGAAGGCTATATTCGCAAAGCTTTAGAT
CAGGTTGCTAAGATGAAACGCATGGAAGAAAAACGTATTCCTGCAAATATTGATTGGGATGCTATTGATTCTATTGCAAC
TGAGGCGCGTCAGAAATTTAAGAAAATTAATCCTGAAACAATTGGACAAGCTAGTCGTATTTCAGGAGTCAATCCTGCGG
ACATCAGTATCTTGATGATTTATTTAGAAGGAAACGGAAAAGCTCATCGAAAATATTAA

Upstream 100 bases:

>100_bases
ACCGAGTTGGCTAAATGGCCAGGACATATTGTCCGCATTGAAGGCTAAACTAAAGCGTATGATTTTAAATGAATAGACTG
AAATAAAGGATTTTAAAAAA

Downstream 100 bases:

>100_bases
ATGGAAATGAAATTGTAATCTCAAAGCAAGTGGAAGCTCTGTATTTCAGGGCTTTTTTATGGTATAATGTGTGCTTAAGA
GGTTATAAGATGAAAAAATT

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 632; Mature: 631

Protein sequence:

>632_residues
MTHEFTESYDVIVIGAGHAGVEASLATSRMGCKTLLATINLDMLAFMPCNPSIGGSAKGIVVREIDALGGEMSKNIDKTY
IQMKMLNTGKGPAVRALRAQADKSLYAREMKHTVEKQANLTLRQTMIDDILVEDGRVVGVLTATGQKFAAKAVVVTTGTA
LRGEIILGELKYSSGPNNSLASVTLADNLKKLGLEIGRFKTGTPPRVKASSINYDQTEIQPGDDKPNHFSFMSKDADYLK
DQIPCWLTYTNQTSHDIINQNLYRAPMFSGIVKGVGPRYCPSIEDKIVRFADKERHQLFLEPEGRDTEEVYVQGLSTSLP
EDVQKDLIHSIKGLEKAEMMRTGYAIEYDIVLPHQLRATLETKLISGLFTAGQTNGTSGYEEAAGQGLIAGINAALKVQG
KPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMRLTEIGRDIGLVDDERWKAFEIKKNQFDNEL
KRLNSIKLKPVKATNDRVQELGFKPLTDAMTAKEFMRRPEIDYATAVSFVGPAAEDLDAKIIELLETEIKYEGYIRKALD
QVAKMKRMEEKRIPANIDWDAIDSIATEARQKFKKINPETIGQASRISGVNPADISILMIYLEGNGKAHRKY

Sequences:

>Translated_632_residues
MTHEFTESYDVIVIGAGHAGVEASLATSRMGCKTLLATINLDMLAFMPCNPSIGGSAKGIVVREIDALGGEMSKNIDKTY
IQMKMLNTGKGPAVRALRAQADKSLYAREMKHTVEKQANLTLRQTMIDDILVEDGRVVGVLTATGQKFAAKAVVVTTGTA
LRGEIILGELKYSSGPNNSLASVTLADNLKKLGLEIGRFKTGTPPRVKASSINYDQTEIQPGDDKPNHFSFMSKDADYLK
DQIPCWLTYTNQTSHDIINQNLYRAPMFSGIVKGVGPRYCPSIEDKIVRFADKERHQLFLEPEGRDTEEVYVQGLSTSLP
EDVQKDLIHSIKGLEKAEMMRTGYAIEYDIVLPHQLRATLETKLISGLFTAGQTNGTSGYEEAAGQGLIAGINAALKVQG
KPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMRLTEIGRDIGLVDDERWKAFEIKKNQFDNEL
KRLNSIKLKPVKATNDRVQELGFKPLTDAMTAKEFMRRPEIDYATAVSFVGPAAEDLDAKIIELLETEIKYEGYIRKALD
QVAKMKRMEEKRIPANIDWDAIDSIATEARQKFKKINPETIGQASRISGVNPADISILMIYLEGNGKAHRKY
>Mature_631_residues
THEFTESYDVIVIGAGHAGVEASLATSRMGCKTLLATINLDMLAFMPCNPSIGGSAKGIVVREIDALGGEMSKNIDKTYI
QMKMLNTGKGPAVRALRAQADKSLYAREMKHTVEKQANLTLRQTMIDDILVEDGRVVGVLTATGQKFAAKAVVVTTGTAL
RGEIILGELKYSSGPNNSLASVTLADNLKKLGLEIGRFKTGTPPRVKASSINYDQTEIQPGDDKPNHFSFMSKDADYLKD
QIPCWLTYTNQTSHDIINQNLYRAPMFSGIVKGVGPRYCPSIEDKIVRFADKERHQLFLEPEGRDTEEVYVQGLSTSLPE
DVQKDLIHSIKGLEKAEMMRTGYAIEYDIVLPHQLRATLETKLISGLFTAGQTNGTSGYEEAAGQGLIAGINAALKVQGK
PELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMRLTEIGRDIGLVDDERWKAFEIKKNQFDNELK
RLNSIKLKPVKATNDRVQELGFKPLTDAMTAKEFMRRPEIDYATAVSFVGPAAEDLDAKIIELLETEIKYEGYIRKALDQ
VAKMKRMEEKRIPANIDWDAIDSIATEARQKFKKINPETIGQASRISGVNPADISILMIYLEGNGKAHRKY

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=633, Percent_Identity=40.1263823064771, Blast_Score=438, Evalue=1e-123,
Organism=Homo sapiens, GI19882217, Length=658, Percent_Identity=38.6018237082067, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI183227703, Length=673, Percent_Identity=37.741456166419, Blast_Score=419, Evalue=1e-117,
Organism=Escherichia coli, GI2367273, Length=626, Percent_Identity=49.2012779552716, Blast_Score=620, Evalue=1e-179,
Organism=Caenorhabditis elegans, GI17534255, Length=622, Percent_Identity=38.7459807073955, Blast_Score=444, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6321202, Length=622, Percent_Identity=39.871382636656, Blast_Score=443, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24658174, Length=632, Percent_Identity=42.0886075949367, Blast_Score=452, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 70158; Mature: 70026

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHEFTESYDVIVIGAGHAGVEASLATSRMGCKTLLATINLDMLAFMPCNPSIGGSAKGI
CCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCE
VVREIDALGGEMSKNIDKTYIQMKMLNTGKGPAVRALRAQADKSLYAREMKHTVEKQANL
EEEEHHHHCCHHHCCCCHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TLRQTMIDDILVEDGRVVGVLTATGQKFAAKAVVVTTGTALRGEIILGELKYSSGPNNSL
HHHHHHHHHHHHCCCCEEEEEEECCCCHHCEEEEEECCCEEECEEEEEEEECCCCCCCCE
ASVTLADNLKKLGLEIGRFKTGTPPRVKASSINYDQTEIQPGDDKPNHFSFMSKDADYLK
EEEEHHHHHHHHCHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHCCCHHHHH
DQIPCWLTYTNQTSHDIINQNLYRAPMFSGIVKGVGPRYCPSIEDKIVRFADKERHQLFL
HCCCEEEEECCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEE
EPEGRDTEEVYVQGLSTSLPEDVQKDLIHSIKGLEKAEMMRTGYAIEYDIVLPHQLRATL
CCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEECHHHHHHHH
ETKLISGLFTAGQTNGTSGYEEAAGQGLIAGINAALKVQGKPELILKRSDAYIGVMIDDL
HHHHHHHHHHCCCCCCCCCHHHHCCCCEEEECCEEEEECCCCCEEEEECCCEEEEEHHHH
VTKGTLEPYRLLTSRAEYRLILRHDNADMRLTEIGRDIGLVDDERWKAFEIKKNQFDNEL
HHCCCCCHHHHHHCCCCEEEEEEECCCCEEHHHHCCCCCCCCCCCCEEEEEHHHHHHHHH
KRLNSIKLKPVKATNDRVQELGFKPLTDAMTAKEFMRRPEIDYATAVSFVGPAAEDLDAK
HHHHCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHH
IIELLETEIKYEGYIRKALDQVAKMKRMEEKRIPANIDWDAIDSIATEARQKFKKINPET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHH
IGQASRISGVNPADISILMIYLEGNGKAHRKY
CCCHHHCCCCCCCCEEEEEEEEECCCCCCCCC
>Mature Secondary Structure 
THEFTESYDVIVIGAGHAGVEASLATSRMGCKTLLATINLDMLAFMPCNPSIGGSAKGI
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCE
VVREIDALGGEMSKNIDKTYIQMKMLNTGKGPAVRALRAQADKSLYAREMKHTVEKQANL
EEEEHHHHCCHHHCCCCHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TLRQTMIDDILVEDGRVVGVLTATGQKFAAKAVVVTTGTALRGEIILGELKYSSGPNNSL
HHHHHHHHHHHHCCCCEEEEEEECCCCHHCEEEEEECCCEEECEEEEEEEECCCCCCCCE
ASVTLADNLKKLGLEIGRFKTGTPPRVKASSINYDQTEIQPGDDKPNHFSFMSKDADYLK
EEEEHHHHHHHHCHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHCCCHHHHH
DQIPCWLTYTNQTSHDIINQNLYRAPMFSGIVKGVGPRYCPSIEDKIVRFADKERHQLFL
HCCCEEEEECCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEE
EPEGRDTEEVYVQGLSTSLPEDVQKDLIHSIKGLEKAEMMRTGYAIEYDIVLPHQLRATL
CCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEECHHHHHHHH
ETKLISGLFTAGQTNGTSGYEEAAGQGLIAGINAALKVQGKPELILKRSDAYIGVMIDDL
HHHHHHHHHHCCCCCCCCCHHHHCCCCEEEECCEEEEECCCCCEEEEECCCEEEEEHHHH
VTKGTLEPYRLLTSRAEYRLILRHDNADMRLTEIGRDIGLVDDERWKAFEIKKNQFDNEL
HHCCCCCHHHHHHCCCCEEEEEEECCCCEEHHHHCCCCCCCCCCCCEEEEEHHHHHHHHH
KRLNSIKLKPVKATNDRVQELGFKPLTDAMTAKEFMRRPEIDYATAVSFVGPAAEDLDAK
HHHHCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHH
IIELLETEIKYEGYIRKALDQVAKMKRMEEKRIPANIDWDAIDSIATEARQKFKKINPET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHH
IGQASRISGVNPADISILMIYLEGNGKAHRKY
CCCHHHCCCCCCCCEEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]