Click here to switch to the map view.

The map label for this gene is hisS [H]

Identifier: 19746998

GI number: 19746998

Start: 1840495

End: 1841775

Strand: Reverse

Name: hisS [H]

Synonym: spyM18_2189

Alternate gene names: 19746998

Gene position: 1841775-1840495 (Counterclockwise)

Preceding gene: 19747008

Following gene: 19746997

Centisome position: 97.19

GC content: 41.69

Gene sequence:

>1281_bases
ATGAAACTTCAAAAACCAAAAGGGACTCAGGATATTCTGCCTGGGGATGCTGCGAAATGGCAGTATGTGGAATCTGTTGC
GCGTGATACGTTTAGCCAATACAATTATGGAGAAATTCGTACTCCTATGTTTGAACACTACGAGGTAATCTCGCGGTCTG
TGGGTGATACGACTGATATTGTGACGAAGGAAATGTATGATTTCTATGATAAAGGAGATCGTCACATTACCTTGCGACCA
GAGGGTACAGCTCCAGTGGTGCGTTCTTATGTTGAAAATAAATTGTTTGCGCCAGAGGTCCAAAAACCCGTCAAACTTTA
TTATATCGGTTCCATGTTCCGCTACGAACGTCCTCAAGCTGGTCGTTTACGTGAATTTCACCAGATTGGGGTAGAATGTT
TCGGCGCTGCTAATCCAGCAACAGATGTTGAAACCATTGCGATGGCCTATCACCTCTTTGAAAAATTGGGCATTAAGGAT
GTCACCCTTCATTTAAATAGTTTGGGGAGTCCTGAGAGTCGTGCAGCTTATCGTCAAGCCTTGATTGACTATTTAACACC
AATGCGTGATCAATTATCTAAAGATAGTCAACGTCGCTTGGATGAAAACCCACTGCGCGTGCTTGATTCTAAAGAAAAAG
AAGATAAACTAGCCGTTGAAAAAGCGCCTTCTATTTTGGATTACTTAGATGAGGAAAGTCAGGCGCACTTTGAGGCGGTC
AAAGATATGCTTGAAGCACTTAACATTCCTTATGTGATTGACACCAATATGGTGCGTGGTCTTGATTACTATAACCACAC
TATTTTTGAATTCATCACGTCTGTGGAAGGATCTGACTTAACTATCTGTGCGGGTGGACGTTATGATAGTTTGGTAGGTT
ATTTTGGTGGGCCTGAGACTCCAGGATTTGGATTTGGTCTTGGTTTAGAACGTCTCTTGATGGTTATTGAAAAGCAGGGC
ATTACCTTGCCAATTGAGACAGAGATGGATGTTTACCTTGCTGTGTTAGGTGATGGTGCTAATAGCAAAGCTTTAGAATT
GGTTCAAGCCATTCGCCGTCAAGGGTTTACAGCTGAACGAGATTACCTTGGCCGTAAGATTAAGGCTCAGTTTAAATCAG
CCGATACCTTTAAAGCAAAATTGGTAATGACTTTAGGAGAAAGTGAAGTTGAAGCAGGCAAAGCTGTGATTAAGAATAAT
CGTAGTCGTCAAGAAGTGGAAGTCAGTTTTGAAGATATGATGACAAACTTTGCGAACATCTCAGAGCAATTATTATCTTA
A

Upstream 100 bases:

>100_bases
GAAAATACTATCAAACTAAAGCAAATGATATATGCTTGTTTAAAATATAAGAAAAAATGGTAGAATGTTTAAAGAGAAAA
CTAATCAAAGAGGTAAACTT

Downstream 100 bases:

>100_bases
TGATTTTATTGTCGTCATAAGCTTGTGTTGATATGATTGTTTAGAAGATAATCACTAAAACAGGTGTAACTTCTCTTTTT
AGAAGGGAGTTGCACCTGTT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKLQKPKGTQDILPGDAAKWQYVESVARDTFSQYNYGEIRTPMFEHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRP
EGTAPVVRSYVENKLFAPEVQKPVKLYYIGSMFRYERPQAGRLREFHQIGVECFGAANPATDVETIAMAYHLFEKLGIKD
VTLHLNSLGSPESRAAYRQALIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLAVEKAPSILDYLDEESQAHFEAV
KDMLEALNIPYVIDTNMVRGLDYYNHTIFEFITSVEGSDLTICAGGRYDSLVGYFGGPETPGFGFGLGLERLLMVIEKQG
ITLPIETEMDVYLAVLGDGANSKALELVQAIRRQGFTAERDYLGRKIKAQFKSADTFKAKLVMTLGESEVEAGKAVIKNN
RSRQEVEVSFEDMMTNFANISEQLLS

Sequences:

>Translated_426_residues
MKLQKPKGTQDILPGDAAKWQYVESVARDTFSQYNYGEIRTPMFEHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRP
EGTAPVVRSYVENKLFAPEVQKPVKLYYIGSMFRYERPQAGRLREFHQIGVECFGAANPATDVETIAMAYHLFEKLGIKD
VTLHLNSLGSPESRAAYRQALIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLAVEKAPSILDYLDEESQAHFEAV
KDMLEALNIPYVIDTNMVRGLDYYNHTIFEFITSVEGSDLTICAGGRYDSLVGYFGGPETPGFGFGLGLERLLMVIEKQG
ITLPIETEMDVYLAVLGDGANSKALELVQAIRRQGFTAERDYLGRKIKAQFKSADTFKAKLVMTLGESEVEAGKAVIKNN
RSRQEVEVSFEDMMTNFANISEQLLS
>Mature_426_residues
MKLQKPKGTQDILPGDAAKWQYVESVARDTFSQYNYGEIRTPMFEHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRP
EGTAPVVRSYVENKLFAPEVQKPVKLYYIGSMFRYERPQAGRLREFHQIGVECFGAANPATDVETIAMAYHLFEKLGIKD
VTLHLNSLGSPESRAAYRQALIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLAVEKAPSILDYLDEESQAHFEAV
KDMLEALNIPYVIDTNMVRGLDYYNHTIFEFITSVEGSDLTICAGGRYDSLVGYFGGPETPGFGFGLGLERLLMVIEKQG
ITLPIETEMDVYLAVLGDGANSKALELVQAIRRQGFTAERDYLGRKIKAQFKSADTFKAKLVMTLGESEVEAGKAVIKNN
RSRQEVEVSFEDMMTNFANISEQLLS

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI15029520, Length=473, Percent_Identity=24.9471458773784, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI6996014, Length=469, Percent_Identity=25.7995735607676, Blast_Score=115, Evalue=6e-26,
Organism=Escherichia coli, GI1788861, Length=411, Percent_Identity=43.30900243309, Blast_Score=357, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI71993693, Length=461, Percent_Identity=26.6811279826464, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71993686, Length=461, Percent_Identity=26.6811279826464, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6325290, Length=469, Percent_Identity=24.0938166311301, Blast_Score=103, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24643061, Length=468, Percent_Identity=25.4273504273504, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24643059, Length=487, Percent_Identity=24.6406570841889, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 48199; Mature: 48199

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQKPKGTQDILPGDAAKWQYVESVARDTFSQYNYGEIRTPMFEHYEVISRSVGDTTDI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHH
VTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQKPVKLYYIGSMFRYERPQA
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHCCCCCC
GRLREFHQIGVECFGAANPATDVETIAMAYHLFEKLGIKDVTLHLNSLGSPESRAAYRQA
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHH
LIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLAVEKAPSILDYLDEESQAHFEAV
HHHHHHHHHHHHCCHHHHHCCCCCHHHHCCCCHHHHHHHHHCCHHHHHHCCHHHHHHHHH
KDMLEALNIPYVIDTNMVRGLDYYNHTIFEFITSVEGSDLTICAGGRYDSLVGYFGGPET
HHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHEECCCCCC
PGFGFGLGLERLLMVIEKQGITLPIETEMDVYLAVLGDGANSKALELVQAIRRQGFTAER
CCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCHH
DYLGRKIKAQFKSADTFKAKLVMTLGESEVEAGKAVIKNNRSRQEVEVSFEDMMTNFANI
HHHHHHHHHHHCCCCHHHEEEEEEECCHHHHHHHHHHHCCCCHHHHHEEHHHHHHHHHHH
SEQLLS
HHHHCC
>Mature Secondary Structure
MKLQKPKGTQDILPGDAAKWQYVESVARDTFSQYNYGEIRTPMFEHYEVISRSVGDTTDI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHH
VTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQKPVKLYYIGSMFRYERPQA
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHCCCCCC
GRLREFHQIGVECFGAANPATDVETIAMAYHLFEKLGIKDVTLHLNSLGSPESRAAYRQA
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHH
LIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLAVEKAPSILDYLDEESQAHFEAV
HHHHHHHHHHHHCCHHHHHCCCCCHHHHCCCCHHHHHHHHHCCHHHHHHCCHHHHHHHHH
KDMLEALNIPYVIDTNMVRGLDYYNHTIFEFITSVEGSDLTICAGGRYDSLVGYFGGPET
HHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHEECCCCCC
PGFGFGLGLERLLMVIEKQGITLPIETEMDVYLAVLGDGANSKALELVQAIRRQGFTAER
CCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCHH
DYLGRKIKAQFKSADTFKAKLVMTLGESEVEAGKAVIKNNRSRQEVEVSFEDMMTNFANI
HHHHHHHHHHHCCCCHHHEEEEEEECCHHHHHHHHHHHCCCCHHHHHEEHHHHHHHHHHH
SEQLLS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]