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The map label for this gene is pbpF [H]

Identifier: 19746940

GI number: 19746940

Start: 1769083

End: 1771419

Strand: Reverse

Name: pbpF [H]

Synonym: spyM18_2120

Alternate gene names: 19746940

Gene position: 1771419-1769083 (Counterclockwise)

Preceding gene: 19746941

Following gene: 19746939

Centisome position: 93.48

GC content: 40.14

Gene sequence:

>2337_bases
ATGAGATTTCTAGAACTTTTACAAAAGAAATTTTTTCCTAAAGCATATCAGGAAAAACAATTCTTAATGCATCAAAAAAC
GCGTTTAACGCCACAACACAATCAAAAGCAGTATTCGCCAAATGCCAATCATTTGGACTCATCAGCTACCAAAAACTCAG
AACAAGACCCTGCAATAGCTCTGCAACGCAGTAGAGCCTACGAAGGAAGCCCTAAAAGTCGGCCCGCTTGGTTGCAAAAG
CTGGAAGCTGTTTTGCCGTCTCCTCAACATCCAATTCGGCGTTTTTGGCGCCGCTATCACATCGGAAAACTGCTAATGAT
TCTGATTGGAACTCTTGTCTTACTCTTAGGATCATACTTGTTTTACTTATCAAAAACAGCTAAAGTATCTGATTTACAAG
ATGCCTTGAAGGCTACAACGGTTATTTATGATCACAAAGGAGAGTATGCAGGCAGTTTATCTGGTCAAAAAGGGAGTTAT
GTTGAGCTCAACGCTATTTCAGATGATCTTGAGAATGCTGTTATTGCCACTGAGGATAGGACTTTTTACAGTAATAGCGG
TATTAATCTTAAACGCTTCTTATTGGCGGTAGTTACGGCGGGCCGCTTTGGAGGTGGCTCAACGATTACACAGCAACTGG
CTAAAAATGCTTATCTCTCACAAGATCAGACAATTAAACGAAAGGCCCGAGAGTTTTTTTTGGCGTTAGAGTTGACCAAA
AAATACAGTAAAAAAGATATTCTTACTATGTACCTTAACAACTCCTATTTTGGAAATGGAGTTTGGGGAGTTGAAGATGC
CAGTCAAAAATATTTTGGAACCACAGCTGCTAACTTAACACTGGATGAAGCTGCCACATTAGCAGGTATGCTCAAAGGAC
CTGAAATATATAACCCTTACCATTCTCTAAAAAATGCTACTCACCGTAGAGATACTGTTTTAGGAGCGATGGTTGATGCC
AAAAAGATTACCCAAACAAAAGCTCAGCAAGCTAGAGCAGTAGGGCTAAAAAATCGCTTAGCTGATACTTATGTTGGTAA
GACAGATGACTACAAATACCCATCCTACTTTGATGCTGTTATTAGTGAAGCAATAGCAACTTATGGTCTTTCAGAAAAAG
ACATTGTTAATAATGGATACAAAGTTTACACCGAGCTAGATCAAAATTACCAAACTGGCATGCAGACGACTTTTAACAAC
GATGAACTATTTCCTGTTTCAGCTTATGACGGTAGCTCTGCTCAAGCAGCTAGTGTTGCTTTAGATCCTAAAACAGGAGG
TGTTAGAGGTCTGATTGGTCGTGTGAATAGTAGTGAAAATCCGACTTTCAGAAGTTTTAACTATGCGACTCAAGCAAAAC
GTAGTCCCGCATCAACAATCAAACCACTCGTGGTTTACGCGCCAGCCGTTGCTTCAGGATGGTCAATTGAAAAAGAACTA
CCAAATACCGTTCAAGATTTCGATGGCTATCAGCCACATAATTATGGAAATTATGAATCAGAAGATGTTCCTATGTATCA
AGCATTAGCAAACTCTTATAATATTCCAGCAGTTTCTACATTGAACGATATCGGAATCGATAAAGCCTTTACCTATGGTA
AAACATTTGGGTTAGATATGAGCTCTGCCAAAAAAGAGTTGGGGGTAGCTTTAGGTGGCAACGTGACAACCAATCCATTG
GAGATGGCTCAGGCATATGCTGCCTTTGCCAATAATGGAGTAATCCATCCTGCGCACTTGATTAACCGGATTGAAAATGC
CAGGGGTGAAGTGCTTAAAACCTTTACTGATAAGGCTAAACGTGTTGTCAGCCAGTCTGTTGCAGATAAGATGACAGCCA
TGATGCTAGGTACCTTTTCAAATGGAACAGCAGTCAATGCTAACGTATATGGCTATACACTAGCTGGTAAAACAGGGACG
ACAGAAACCAACTTCAATCCCGACTTAGCAGGCGATCAGTGGGTTATTGGTTATACACCAGATGTTGTTATTAGTCAATG
GGTAGGATTTAATCAGACCGATGAAAATCATTATCTAACGGATTCAAGTGCAGGCACGGCCTCAGCTATTTTTAGCACTC
AGGCATCTTACATTTTGCCTTATACCAAGGGCAGCCAATTTCATGTAGATAACGCCTACGCTCAAAATGGTATTTCAGCT
GTTTATGGAGTCAATGAAACAGGTAATCAATCAGGAGTTGACACTCAATCTATTATTGATGGTTTAAGAAAATCAGCACA
AGAAGCTTCGCAATCACTATCAAAAGCAGTCGATCAGTCAGGGTTACGTGATAAAGCCCAATCTATTTGGAAAGAGATTG
TTGACTATTTTAGATAG

Upstream 100 bases:

>100_bases
CACAAGGACTACTCTTTCAAGTGGATGGTGTGGCTTATTCAAAGCATATTTCGATGACCTAATCAAGGTTACTTTAAGAT
CATAGTGGTATAATAACGTT

Downstream 100 bases:

>100_bases
CTTGCAGTATTTTAGAGATCGTGTTAAAATAATAGTTACGGAGGCGTTATGGCACAGAAAAAAGCGAGTCTAGCGTGTGT
CGAATGTGGCAGTCGTAACT

Product: penicillin-binding protein 2a

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 778; Mature: 778

Protein sequence:

>778_residues
MRFLELLQKKFFPKAYQEKQFLMHQKTRLTPQHNQKQYSPNANHLDSSATKNSEQDPAIALQRSRAYEGSPKSRPAWLQK
LEAVLPSPQHPIRRFWRRYHIGKLLMILIGTLVLLLGSYLFYLSKTAKVSDLQDALKATTVIYDHKGEYAGSLSGQKGSY
VELNAISDDLENAVIATEDRTFYSNSGINLKRFLLAVVTAGRFGGGSTITQQLAKNAYLSQDQTIKRKAREFFLALELTK
KYSKKDILTMYLNNSYFGNGVWGVEDASQKYFGTTAANLTLDEAATLAGMLKGPEIYNPYHSLKNATHRRDTVLGAMVDA
KKITQTKAQQARAVGLKNRLADTYVGKTDDYKYPSYFDAVISEAIATYGLSEKDIVNNGYKVYTELDQNYQTGMQTTFNN
DELFPVSAYDGSSAQAASVALDPKTGGVRGLIGRVNSSENPTFRSFNYATQAKRSPASTIKPLVVYAPAVASGWSIEKEL
PNTVQDFDGYQPHNYGNYESEDVPMYQALANSYNIPAVSTLNDIGIDKAFTYGKTFGLDMSSAKKELGVALGGNVTTNPL
EMAQAYAAFANNGVIHPAHLINRIENARGEVLKTFTDKAKRVVSQSVADKMTAMMLGTFSNGTAVNANVYGYTLAGKTGT
TETNFNPDLAGDQWVIGYTPDVVISQWVGFNQTDENHYLTDSSAGTASAIFSTQASYILPYTKGSQFHVDNAYAQNGISA
VYGVNETGNQSGVDTQSIIDGLRKSAQEASQSLSKAVDQSGLRDKAQSIWKEIVDYFR

Sequences:

>Translated_778_residues
MRFLELLQKKFFPKAYQEKQFLMHQKTRLTPQHNQKQYSPNANHLDSSATKNSEQDPAIALQRSRAYEGSPKSRPAWLQK
LEAVLPSPQHPIRRFWRRYHIGKLLMILIGTLVLLLGSYLFYLSKTAKVSDLQDALKATTVIYDHKGEYAGSLSGQKGSY
VELNAISDDLENAVIATEDRTFYSNSGINLKRFLLAVVTAGRFGGGSTITQQLAKNAYLSQDQTIKRKAREFFLALELTK
KYSKKDILTMYLNNSYFGNGVWGVEDASQKYFGTTAANLTLDEAATLAGMLKGPEIYNPYHSLKNATHRRDTVLGAMVDA
KKITQTKAQQARAVGLKNRLADTYVGKTDDYKYPSYFDAVISEAIATYGLSEKDIVNNGYKVYTELDQNYQTGMQTTFNN
DELFPVSAYDGSSAQAASVALDPKTGGVRGLIGRVNSSENPTFRSFNYATQAKRSPASTIKPLVVYAPAVASGWSIEKEL
PNTVQDFDGYQPHNYGNYESEDVPMYQALANSYNIPAVSTLNDIGIDKAFTYGKTFGLDMSSAKKELGVALGGNVTTNPL
EMAQAYAAFANNGVIHPAHLINRIENARGEVLKTFTDKAKRVVSQSVADKMTAMMLGTFSNGTAVNANVYGYTLAGKTGT
TETNFNPDLAGDQWVIGYTPDVVISQWVGFNQTDENHYLTDSSAGTASAIFSTQASYILPYTKGSQFHVDNAYAQNGISA
VYGVNETGNQSGVDTQSIIDGLRKSAQEASQSLSKAVDQSGLRDKAQSIWKEIVDYFR
>Mature_778_residues
MRFLELLQKKFFPKAYQEKQFLMHQKTRLTPQHNQKQYSPNANHLDSSATKNSEQDPAIALQRSRAYEGSPKSRPAWLQK
LEAVLPSPQHPIRRFWRRYHIGKLLMILIGTLVLLLGSYLFYLSKTAKVSDLQDALKATTVIYDHKGEYAGSLSGQKGSY
VELNAISDDLENAVIATEDRTFYSNSGINLKRFLLAVVTAGRFGGGSTITQQLAKNAYLSQDQTIKRKAREFFLALELTK
KYSKKDILTMYLNNSYFGNGVWGVEDASQKYFGTTAANLTLDEAATLAGMLKGPEIYNPYHSLKNATHRRDTVLGAMVDA
KKITQTKAQQARAVGLKNRLADTYVGKTDDYKYPSYFDAVISEAIATYGLSEKDIVNNGYKVYTELDQNYQTGMQTTFNN
DELFPVSAYDGSSAQAASVALDPKTGGVRGLIGRVNSSENPTFRSFNYATQAKRSPASTIKPLVVYAPAVASGWSIEKEL
PNTVQDFDGYQPHNYGNYESEDVPMYQALANSYNIPAVSTLNDIGIDKAFTYGKTFGLDMSSAKKELGVALGGNVTTNPL
EMAQAYAAFANNGVIHPAHLINRIENARGEVLKTFTDKAKRVVSQSVADKMTAMMLGTFSNGTAVNANVYGYTLAGKTGT
TETNFNPDLAGDQWVIGYTPDVVISQWVGFNQTDENHYLTDSSAGTASAIFSTQASYILPYTKGSQFHVDNAYAQNGISA
VYGVNETGNQSGVDTQSIIDGLRKSAQEASQSLSKAVDQSGLRDKAQSIWKEIVDYFR

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=538, Percent_Identity=30.6691449814126, Blast_Score=176, Evalue=7e-45,
Organism=Escherichia coli, GI87082258, Length=315, Percent_Identity=33.015873015873, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI1788867, Length=426, Percent_Identity=28.169014084507, Blast_Score=124, Evalue=3e-29,
Organism=Escherichia coli, GI1789601, Length=139, Percent_Identity=41.726618705036, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 85676; Mature: 85676

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLELLQKKFFPKAYQEKQFLMHQKTRLTPQHNQKQYSPNANHLDSSATKNSEQDPAIA
CHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
LQRSRAYEGSPKSRPAWLQKLEAVLPSPQHPIRRFWRRYHIGKLLMILIGTLVLLLGSYL
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYLSKTAKVSDLQDALKATTVIYDHKGEYAGSLSGQKGSYVELNAISDDLENAVIATEDR
HHHHCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCEEEEECCHHHHCCCEEEECCC
TFYSNSGINLKRFLLAVVTAGRFGGGSTITQQLAKNAYLSQDQTIKRKAREFFLALELTK
EEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KYSKKDILTMYLNNSYFGNGVWGVEDASQKYFGTTAANLTLDEAATLAGMLKGPEIYNPY
HCCCCCEEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCHH
HSLKNATHRRDTVLGAMVDAKKITQTKAQQARAVGLKNRLADTYVGKTDDYKYPSYFDAV
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
ISEAIATYGLSEKDIVNNGYKVYTELDQNYQTGMQTTFNNDELFPVSAYDGSSAQAASVA
HHHHHHHCCCCCHHHHCCCEEEEEEHHCCHHHCCCEECCCCCEEEEECCCCCCCCEEEEE
LDPKTGGVRGLIGRVNSSENPTFRSFNYATQAKRSPASTIKPLVVYAPAVASGWSIEKEL
ECCCCCCHHHHHCCCCCCCCCCEECCCHHHHHCCCCHHHCCCEEEECCCCCCCCCCHHHC
PNTVQDFDGYQPHNYGNYESEDVPMYQALANSYNIPAVSTLNDIGIDKAFTYGKTFGLDM
CCHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHCCCCHHHHCCCCCCCCH
SSAKKELGVALGGNVTTNPLEMAQAYAAFANNGVIHPAHLINRIENARGEVLKTFTDKAK
HHHHHHHCEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RVVSQSVADKMTAMMLGTFSNGTAVNANVYGYTLAGKTGTTETNFNPDLAGDQWVIGYTP
HHHHHHHHHHHHHHHHEECCCCCEEECEEEEEEEECCCCCCCCCCCCCCCCCCEEEECCH
DVVISQWVGFNQTDENHYLTDSSAGTASAIFSTQASYILPYTKGSQFHVDNAYAQNGISA
HHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHCCCCEEEEECCCCEEEECHHHHHCCCEE
VYGVNETGNQSGVDTQSIIDGLRKSAQEASQSLSKAVDQSGLRDKAQSIWKEIVDYFR
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MRFLELLQKKFFPKAYQEKQFLMHQKTRLTPQHNQKQYSPNANHLDSSATKNSEQDPAIA
CHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
LQRSRAYEGSPKSRPAWLQKLEAVLPSPQHPIRRFWRRYHIGKLLMILIGTLVLLLGSYL
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYLSKTAKVSDLQDALKATTVIYDHKGEYAGSLSGQKGSYVELNAISDDLENAVIATEDR
HHHHCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCEEEEECCHHHHCCCEEEECCC
TFYSNSGINLKRFLLAVVTAGRFGGGSTITQQLAKNAYLSQDQTIKRKAREFFLALELTK
EEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KYSKKDILTMYLNNSYFGNGVWGVEDASQKYFGTTAANLTLDEAATLAGMLKGPEIYNPY
HCCCCCEEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCHH
HSLKNATHRRDTVLGAMVDAKKITQTKAQQARAVGLKNRLADTYVGKTDDYKYPSYFDAV
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
ISEAIATYGLSEKDIVNNGYKVYTELDQNYQTGMQTTFNNDELFPVSAYDGSSAQAASVA
HHHHHHHCCCCCHHHHCCCEEEEEEHHCCHHHCCCEECCCCCEEEEECCCCCCCCEEEEE
LDPKTGGVRGLIGRVNSSENPTFRSFNYATQAKRSPASTIKPLVVYAPAVASGWSIEKEL
ECCCCCCHHHHHCCCCCCCCCCEECCCHHHHHCCCCHHHCCCEEEECCCCCCCCCCHHHC
PNTVQDFDGYQPHNYGNYESEDVPMYQALANSYNIPAVSTLNDIGIDKAFTYGKTFGLDM
CCHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHCCCCHHHHCCCCCCCCH
SSAKKELGVALGGNVTTNPLEMAQAYAAFANNGVIHPAHLINRIENARGEVLKTFTDKAK
HHHHHHHCEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RVVSQSVADKMTAMMLGTFSNGTAVNANVYGYTLAGKTGTTETNFNPDLAGDQWVIGYTP
HHHHHHHHHHHHHHHHEECCCCCEEECEEEEEEEECCCCCCCCCCCCCCCCCCEEEECCH
DVVISQWVGFNQTDENHYLTDSSAGTASAIFSTQASYILPYTKGSQFHVDNAYAQNGISA
HHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHCCCCEEEEECCCCEEEECHHHHHCCCEE
VYGVNETGNQSGVDTQSIIDGLRKSAQEASQSLSKAVDQSGLRDKAQSIWKEIVDYFR
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]