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The map label for this gene is pflD [H]

Identifier: 19746932

GI number: 19746932

Start: 1760927

End: 1763344

Strand: Reverse

Name: pflD [H]

Synonym: spyM18_2110

Alternate gene names: 19746932

Gene position: 1763344-1760927 (Counterclockwise)

Preceding gene: 19746933

Following gene: 19746931

Centisome position: 93.05

GC content: 44.54

Gene sequence:

>2418_bases
ATGACTGAAACAAAAAGCCCTTATTTTGGACATTTAACAGACAGAATGACTCACTATCGTGAAGCTGTGTTAGACAAGAA
ACCTTATATTGATGCTGAGCGTGCTATTTTAGCCACTGAAGCCTATCAAAAACACCAAAATAAGCCAGCAAACCTCAAAC
GCGCTTACATGCTTCAAACTATTTTGGAAAACATGACCATCTATATTGAGGATGAAAGCCTCATTGCTGGTAACCAAGCC
TCTTCAAACAAGGATGCACCGATTTTCCCTGAATATACTCTGGAATTTGTCCTTAACGAACTTGATCTTTTTGAAAAACG
AGATGGGGATGTCTTCTACATTACCGAAGAAACCAAGCAACAGCTCCGAGACATTGCCCCATTCTGGGAAAACAATAACC
TGCGTGCTCGCTGCGGTGTCTTGCTACCAGAAGAAGTGCAAGTGTACATGGAAACAGGTTTCTTTGGCATGGAAGGCAAG
ATGAACTCTGGGGATGCCCATTTAGCGGTTAATTACCAAAAACTCTTGGAACATGGCTTAAAGGGATTTGAAGAGCGGGC
ACGAGCTGCCAAAGCTGCGCTTGATCTGACCATTCCTGAAAACATTGACAAATACCATTTTTACGACTCTGTTTTTATTG
TCATTGATGCAGTCAAAACTTATGCCAAACGCTATGCCCAATTGGCTAGAGAACTAGCAGAAACCGCTAGTCCAGAACGT
CAAGCAGAATTGTTAGACATCGCGTGCATTTGTGACAAAGTGCCTTATGAACCAGCAGCAACGTTTGCAGAAGCTGTACA
ATCCGTCTGGTTCATTCAGTGTATCTTGCAAATTGAATCAAATGGGCATTCCCTATCTTATGGCCGCTTTGACCAGTACA
TGTATCCTTATGTCAAAGCTGATTTGGAGGCTGGCCGTGAAACAGAAGACACAATCGTTGAGCGTTTGACCAACCTCTGG
ATTAAGACCTTGACCATTAATAAAGTACGGAGCCAAGCCCACACCTTCTCATCAGCAGGGAGTCCACTTTACCAAAACGT
GACGATCGGCGGTCAAACACGCGATAAAAAAGACGCGGTCAACCCATTGTCTTACCTTGTCCTACGGAGTGTGGCTCAAA
CCAAATTGCCACAACCTAACTTGACTGTTCGTTACCACAAAGGCCTAGACAATACCTTTATGAACGAGTGTATCGAGGTC
ATGAAACTTGGTTTTGGTATGCCGGCCATGAACAACGATGAAATCATCATTCCATCTTTCATCAAAAAAGGTGTCTCAGA
AGAAGATGCTTATGATTACTCAGCCATTGGCTGTGTGGAAACAGCAGTTCCTGGAAAATGGGGCTACCGTTGCACGGGGA
TGAGTTACATCAACTTCCCTAAAATTTTGCTGATTACCATGAATGACGGGATTGACCCAGCATCAGGCAAGCGCTTTGCA
AAAGGACACGGTCACTTCAAAGACATGACCTCTTATGAAGAATTAAAAGCAGCATGGGATGCCACCCTGCGCGAAATTAC
CCGCATGAGCGTCATTGTTGAAAATGCCATTGACTTAGGTCTTGAACGTGAAGTACCAGATATTCTTTGCTCAGCCTTGA
CAGATGATTGTATCGGTCGTGGCAAGACCTTAAAAGAAGGCGGAGCTGTTTACGACTATATCTCAGGCCTTCAAGTCGGT
ATTGCCAACCTATCAGACTCCCTAGCAGCGCTGAAAAAATTAGTGTTTGAAGAAGGTCGATTAACTCCAGAAGAACTCTG
GCAGGCCCTTGAAAGTGACTTTGCTGGAGAGCGTGGAGAAGAGATCCGACAAATGCTCATCAATGACGCACCAAAATATG
GTAATGATGATGACTACGCAGATAGTTTAGTCGTCGAAGCTTACGACACCTACATTGATGAAATTGCCAAATACCCAAAT
ACCCGGTATGGCCGTGGCCCAATCGGAGGTATCCGCTATTCTGGGACATCATCAATCTCAGCCAATGTCGGACAAGGAAA
AGGGACCTTGGCGACACCAGACGGTCGCCATGCAGGGACACCCCTAGCAGAAGGCTGTTCACCAGAACACAGCATGGACA
AAAAAGGCCCTACTTCAGTATTGAAATCTGTTGCTAAATTACCAACAGATGAGATTGTCGGAGGAGTACTACTCAACCAA
AAAGTTAACCCACAAACCCTGGCCAAAGAAGAAGACAAACTGAAATTAATGGCCTTACTTCGTACCTTCTTTAATCGCCT
ACACGGTTATCATATCCAGTACAATGTCGTGTCACGTGAAACCTTGATTGATGCCCAAAAACATCCTGAAAAGCACCGCG
ACCTCATTGTCCGTGTTGCCGGCTACTCAGCTTTCTTCAATGTGCTCTCAAAAGCAACCCAAGATGACATTATTGAACGT
ACAGAACACACACTTTAG

Upstream 100 bases:

>100_bases
TATCTCTTCTTATGGTTAAGGATACGCAAAAGTCGCAATATATGATAAAATAGTTACGAAAGAAACATTTATAATTTCGT
AAGAAAGAAGGAGACCCCGC

Downstream 100 bases:

>100_bases
GAGAAAATAAGTATGGAATACATGTTAGACACTTTAGATTTAGAAGCGATTAAAAAATGGCATCACATTTTGCCGCTCGC
AGGTGTGACCTCAAACCCTT

Product: pyruvate formate-lyase 2

Products: NA

Alternate protein names: Pyruvate formate-lyase 3 [H]

Number of amino acids: Translated: 805; Mature: 804

Protein sequence:

>805_residues
MTETKSPYFGHLTDRMTHYREAVLDKKPYIDAERAILATEAYQKHQNKPANLKRAYMLQTILENMTIYIEDESLIAGNQA
SSNKDAPIFPEYTLEFVLNELDLFEKRDGDVFYITEETKQQLRDIAPFWENNNLRARCGVLLPEEVQVYMETGFFGMEGK
MNSGDAHLAVNYQKLLEHGLKGFEERARAAKAALDLTIPENIDKYHFYDSVFIVIDAVKTYAKRYAQLARELAETASPER
QAELLDIACICDKVPYEPAATFAEAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDTIVERLTNLW
IKTLTINKVRSQAHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLRSVAQTKLPQPNLTVRYHKGLDNTFMNECIEV
MKLGFGMPAMNNDEIIIPSFIKKGVSEEDAYDYSAIGCVETAVPGKWGYRCTGMSYINFPKILLITMNDGIDPASGKRFA
KGHGHFKDMTSYEELKAAWDATLREITRMSVIVENAIDLGLEREVPDILCSALTDDCIGRGKTLKEGGAVYDYISGLQVG
IANLSDSLAALKKLVFEEGRLTPEELWQALESDFAGERGEEIRQMLINDAPKYGNDDDYADSLVVEAYDTYIDEIAKYPN
TRYGRGPIGGIRYSGTSSISANVGQGKGTLATPDGRHAGTPLAEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQ
KVNPQTLAKEEDKLKLMALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQDDIIER
TEHTL

Sequences:

>Translated_805_residues
MTETKSPYFGHLTDRMTHYREAVLDKKPYIDAERAILATEAYQKHQNKPANLKRAYMLQTILENMTIYIEDESLIAGNQA
SSNKDAPIFPEYTLEFVLNELDLFEKRDGDVFYITEETKQQLRDIAPFWENNNLRARCGVLLPEEVQVYMETGFFGMEGK
MNSGDAHLAVNYQKLLEHGLKGFEERARAAKAALDLTIPENIDKYHFYDSVFIVIDAVKTYAKRYAQLARELAETASPER
QAELLDIACICDKVPYEPAATFAEAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDTIVERLTNLW
IKTLTINKVRSQAHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLRSVAQTKLPQPNLTVRYHKGLDNTFMNECIEV
MKLGFGMPAMNNDEIIIPSFIKKGVSEEDAYDYSAIGCVETAVPGKWGYRCTGMSYINFPKILLITMNDGIDPASGKRFA
KGHGHFKDMTSYEELKAAWDATLREITRMSVIVENAIDLGLEREVPDILCSALTDDCIGRGKTLKEGGAVYDYISGLQVG
IANLSDSLAALKKLVFEEGRLTPEELWQALESDFAGERGEEIRQMLINDAPKYGNDDDYADSLVVEAYDTYIDEIAKYPN
TRYGRGPIGGIRYSGTSSISANVGQGKGTLATPDGRHAGTPLAEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQ
KVNPQTLAKEEDKLKLMALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQDDIIER
TEHTL
>Mature_804_residues
TETKSPYFGHLTDRMTHYREAVLDKKPYIDAERAILATEAYQKHQNKPANLKRAYMLQTILENMTIYIEDESLIAGNQAS
SNKDAPIFPEYTLEFVLNELDLFEKRDGDVFYITEETKQQLRDIAPFWENNNLRARCGVLLPEEVQVYMETGFFGMEGKM
NSGDAHLAVNYQKLLEHGLKGFEERARAAKAALDLTIPENIDKYHFYDSVFIVIDAVKTYAKRYAQLARELAETASPERQ
AELLDIACICDKVPYEPAATFAEAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDTIVERLTNLWI
KTLTINKVRSQAHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLRSVAQTKLPQPNLTVRYHKGLDNTFMNECIEVM
KLGFGMPAMNNDEIIIPSFIKKGVSEEDAYDYSAIGCVETAVPGKWGYRCTGMSYINFPKILLITMNDGIDPASGKRFAK
GHGHFKDMTSYEELKAAWDATLREITRMSVIVENAIDLGLEREVPDILCSALTDDCIGRGKTLKEGGAVYDYISGLQVGI
ANLSDSLAALKKLVFEEGRLTPEELWQALESDFAGERGEEIRQMLINDAPKYGNDDDYADSLVVEAYDTYIDEIAKYPNT
RYGRGPIGGIRYSGTSSISANVGQGKGTLATPDGRHAGTPLAEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQK
VNPQTLAKEEDKLKLMALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQDDIIERT
EHTL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787044, Length=805, Percent_Identity=53.0434782608696, Blast_Score=855, Evalue=0.0,
Organism=Escherichia coli, GI1790388, Length=798, Percent_Identity=38.0952380952381, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI48994926, Length=590, Percent_Identity=28.3050847457627, Blast_Score=186, Evalue=6e-48,
Organism=Escherichia coli, GI1787131, Length=559, Percent_Identity=27.7280858676207, Blast_Score=186, Evalue=6e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 90329; Mature: 90198

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETKSPYFGHLTDRMTHYREAVLDKKPYIDAERAILATEAYQKHQNKPANLKRAYMLQT
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ILENMTIYIEDESLIAGNQASSNKDAPIFPEYTLEFVLNELDLFEKRDGDVFYITEETKQ
HHCCCEEEEECCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHH
QLRDIAPFWENNNLRARCGVLLPEEVQVYMETGFFGMEGKMNSGDAHLAVNYQKLLEHGL
HHHHHCCCCCCCCCEEEECCCCHHHHHHHHHCCEECCCCCCCCCCCEEEECHHHHHHHHH
KGFEERARAAKAALDLTIPENIDKYHFYDSVFIVIDAVKTYAKRYAQLARELAETASPER
HHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QAELLDIACICDKVPYEPAATFAEAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
DLEAGRETEDTIVERLTNLWIKTLTINKVRSQAHTFSSAGSPLYQNVTIGGQTRDKKDAV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHH
NPLSYLVLRSVAQTKLPQPNLTVRYHKGLDNTFMNECIEVMKLGFGMPAMNNDEIIIPSF
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEHHH
IKKGVSEEDAYDYSAIGCVETAVPGKWGYRCTGMSYINFPKILLITMNDGIDPASGKRFA
HHCCCCCCCCCCHHHHHHHHHCCCCCCCCEECCCCEECCCEEEEEEECCCCCCCCCCCCC
KGHGHFKDMTSYEELKAAWDATLREITRMSVIVENAIDLGLEREVPDILCSALTDDCIGR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
GKTLKEGGAVYDYISGLQVGIANLSDSLAALKKLVFEEGRLTPEELWQALESDFAGERGE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHH
EIRQMLINDAPKYGNDDDYADSLVVEAYDTYIDEIAKYPNTRYGRGPIGGIRYSGTSSIS
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCC
ANVGQGKGTLATPDGRHAGTPLAEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCC
KVNPQTLAKEEDKLKLMALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVA
CCCHHHHHCHHHHHHHHHHHHHHHHHHCCEEEEEEEECHHHHHCCHHCCHHHHCEEEEEH
GYSAFFNVLSKATQDDIIERTEHTL
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TETKSPYFGHLTDRMTHYREAVLDKKPYIDAERAILATEAYQKHQNKPANLKRAYMLQT
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ILENMTIYIEDESLIAGNQASSNKDAPIFPEYTLEFVLNELDLFEKRDGDVFYITEETKQ
HHCCCEEEEECCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHH
QLRDIAPFWENNNLRARCGVLLPEEVQVYMETGFFGMEGKMNSGDAHLAVNYQKLLEHGL
HHHHHCCCCCCCCCEEEECCCCHHHHHHHHHCCEECCCCCCCCCCCEEEECHHHHHHHHH
KGFEERARAAKAALDLTIPENIDKYHFYDSVFIVIDAVKTYAKRYAQLARELAETASPER
HHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QAELLDIACICDKVPYEPAATFAEAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
DLEAGRETEDTIVERLTNLWIKTLTINKVRSQAHTFSSAGSPLYQNVTIGGQTRDKKDAV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHH
NPLSYLVLRSVAQTKLPQPNLTVRYHKGLDNTFMNECIEVMKLGFGMPAMNNDEIIIPSF
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEHHH
IKKGVSEEDAYDYSAIGCVETAVPGKWGYRCTGMSYINFPKILLITMNDGIDPASGKRFA
HHCCCCCCCCCCHHHHHHHHHCCCCCCCCEECCCCEECCCEEEEEEECCCCCCCCCCCCC
KGHGHFKDMTSYEELKAAWDATLREITRMSVIVENAIDLGLEREVPDILCSALTDDCIGR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
GKTLKEGGAVYDYISGLQVGIANLSDSLAALKKLVFEEGRLTPEELWQALESDFAGERGE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHH
EIRQMLINDAPKYGNDDDYADSLVVEAYDTYIDEIAKYPNTRYGRGPIGGIRYSGTSSIS
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCC
ANVGQGKGTLATPDGRHAGTPLAEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCC
KVNPQTLAKEEDKLKLMALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVA
CCCHHHHHCHHHHHHHHHHHHHHHHHHCCEEEEEEEECHHHHHCCHHCCHHHHCEEEEEH
GYSAFFNVLSKATQDDIIERTEHTL
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]