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The map label for this gene is rsmE [H]

Identifier: 19746890

GI number: 19746890

Start: 1715217

End: 1715966

Strand: Reverse

Name: rsmE [H]

Synonym: spyM18_2051

Alternate gene names: 19746890

Gene position: 1715966-1715217 (Counterclockwise)

Preceding gene: 19746891

Following gene: 19746889

Centisome position: 90.55

GC content: 43.6

Gene sequence:

>750_bases
ATGCAACAGTATTTTATTAAAGGCAAAGCAGAAAAAAAGGTAACGATTACCGATAAAGATACCATCAAGCACATGTTTCA
AGTGATGCGCTTGGCTGACAAGGCTGAAGTTGTCTTGGTCTTTGATGATGGGGTGAAGTATTTAGCCAAGGTGACCAATA
GTATGGCTCATGAGTTAGAGATTATAGAGGCTTTGCCAGATCAGGTGGAATTGCCTGTTAAGGTGACTATTGCTTCTGGA
TTTCCTAAAGGAGATAAGTTAGACACGATTGCCCAGAAAGTGACTGAGTTGGGAGCCAGTGCTCTGTGGGGCTACCCAGC
AGACTGGTCAGTGGTCAAATGGGATGGCAAAAAATTAGCTAAAAAAGAAGACAAATTGGCTAAGATTGTATTAGGAGCTG
CTGAGCAGAGCAAACGAAATGTGGTGCCAGAAGTGCATCTTTTTGAACAGAAAGCAGAGTTTCTAAAAAGCCTTTCTAGC
TTTGATCATATCTTCATTGCTTATGAGGAAACAGCTAAGGCAGGTCAGCTAGCGACCTTGGCGCGTGAAGTAAAAGAAGT
TAAGCTAGGGGCAAAAATCCTCTTTATCTTTGGACCAGAAGGGGGAATTTCCCCAACTGAAATCACTCAGTTTGAGGCAG
CAGGTGCCATCAAGGTGGGCTTAGGCCCTCGCATTATGCGAGCAGAGACAGCGCCGCTATATGCCTTAAGTGTTCTTAGT
TATGCTTTGGAATTGCAGGCATCAAAATAA

Upstream 100 bases:

>100_bases
TTAGCGCAGGCTTCTTCCTTGAGACGCACATGATTCAAGGTGAGTGGAACGCCCTTGTCTTTAAAAAAACAGATGATATC
TCAGGAGTGATTGGCGGCTA

Downstream 100 bases:

>100_bases
GTAAGCTGTTGCTTACTTTAGAAAGAAGAGGCTAAAGAAACGAACAGGCAGTTCACGATTGTGTCAACGGCCTTTTTTTA
TCGCTTTTTGTTTTTTGAAG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MQQYFIKGKAEKKVTITDKDTIKHMFQVMRLADKAEVVLVFDDGVKYLAKVTNSMAHELEIIEALPDQVELPVKVTIASG
FPKGDKLDTIAQKVTELGASALWGYPADWSVVKWDGKKLAKKEDKLAKIVLGAAEQSKRNVVPEVHLFEQKAEFLKSLSS
FDHIFIAYEETAKAGQLATLAREVKEVKLGAKILFIFGPEGGISPTEITQFEAAGAIKVGLGPRIMRAETAPLYALSVLS
YALELQASK

Sequences:

>Translated_249_residues
MQQYFIKGKAEKKVTITDKDTIKHMFQVMRLADKAEVVLVFDDGVKYLAKVTNSMAHELEIIEALPDQVELPVKVTIASG
FPKGDKLDTIAQKVTELGASALWGYPADWSVVKWDGKKLAKKEDKLAKIVLGAAEQSKRNVVPEVHLFEQKAEFLKSLSS
FDHIFIAYEETAKAGQLATLAREVKEVKLGAKILFIFGPEGGISPTEITQFEAAGAIKVGLGPRIMRAETAPLYALSVLS
YALELQASK
>Mature_249_residues
MQQYFIKGKAEKKVTITDKDTIKHMFQVMRLADKAEVVLVFDDGVKYLAKVTNSMAHELEIIEALPDQVELPVKVTIASG
FPKGDKLDTIAQKVTELGASALWGYPADWSVVKWDGKKLAKKEDKLAKIVLGAAEQSKRNVVPEVHLFEQKAEFLKSLSS
FDHIFIAYEETAKAGQLATLAREVKEVKLGAKILFIFGPEGGISPTEITQFEAAGAIKVGLGPRIMRAETAPLYALSVLS
YALELQASK

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=225, Percent_Identity=28, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27378; Mature: 27378

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQYFIKGKAEKKVTITDKDTIKHMFQVMRLADKAEVVLVFDDGVKYLAKVTNSMAHELE
CCCEEECCCCCCEEEECCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHH
IIEALPDQVELPVKVTIASGFPKGDKLDTIAQKVTELGASALWGYPADWSVVKWDGKKLA
HHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCHHHHCCCCCCCCEEEECCHHHH
KKEDKLAKIVLGAAEQSKRNVVPEVHLFEQKAEFLKSLSSFDHIFIAYEETAKAGQLATL
HHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHH
AREVKEVKLGAKILFIFGPEGGISPTEITQFEAAGAIKVGLGPRIMRAETAPLYALSVLS
HHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHCCCEEEECCCCEEEECCCCHHHHHHHHH
YALELQASK
HHHHHCCCC
>Mature Secondary Structure
MQQYFIKGKAEKKVTITDKDTIKHMFQVMRLADKAEVVLVFDDGVKYLAKVTNSMAHELE
CCCEEECCCCCCEEEECCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHH
IIEALPDQVELPVKVTIASGFPKGDKLDTIAQKVTELGASALWGYPADWSVVKWDGKKLA
HHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCHHHHCCCCCCCCEEEECCHHHH
KKEDKLAKIVLGAAEQSKRNVVPEVHLFEQKAEFLKSLSSFDHIFIAYEETAKAGQLATL
HHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHH
AREVKEVKLGAKILFIFGPEGGISPTEITQFEAAGAIKVGLGPRIMRAETAPLYALSVLS
HHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHCCCEEEECCCCEEEECCCCHHHHHHHHH
YALELQASK
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]