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The map label for this gene is proS [H]

Identifier: 19746872

GI number: 19746872

Start: 1689151

End: 1691007

Strand: Reverse

Name: proS [H]

Synonym: spyM18_2030

Alternate gene names: 19746872

Gene position: 1691007-1689151 (Counterclockwise)

Preceding gene: 19746873

Following gene: 19746871

Centisome position: 89.23

GC content: 43.56

Gene sequence:

>1857_bases
ATGAAACAAAGTAAACTGCTTATCCCAACCTTGCGCGAAATGCCAAGTGATGCCCAGGTTATCAGCCACGCGCTTATGGT
GCGTGCCGGTTATGTGCGCCAAGTTTCTGCTGGTATCTATGCTTATTTACCACTGGCAAATCGTACCATTGAGAAATTCA
AGACCATCATGCGTGAAGAGTTTGAAAAGATCGGTGCTGTTGAAATGTTGGCACCAGCTCTTTTGACAGCTGATCTCTGG
CGTGAATCAGGCCGTTATGAGACCTATGGAGAGGACCTCTATAAGCTTAAAAACCGTGATAACTCAGACTTTATCTTGGG
TCCGACCCACGAAGAAACCTTTACGACTTTGGTGCGTAATGCGGTCAAATCTTACAAGCAATTGCCCTTAAACCTTTATC
AAATTCAGTCTAAGTATCGTGATGAAAAACGTCCGCGTAATGGCTTGCTTCGTACCCGTGAATTTATCATGAAAGACGGC
TATAGTTTCCATCACAACTATGAAGATTTAGATGTGACCTACGAAGATTATCGTCAAGCTTACGAAGCTATTTTTACCAG
AGCTGGTCTAGATTTCAAAGGGATTATTGGAGATGGCGGTGCCATGGGGGGGAAAGATTCCCAAGAATTTATGGCCATTA
CACCAGCTCGTACGGACCTTGATCGCTGGGTGGTTCTTGACAAGTCTATTGCGTCAATGGATGATATTCCAAAAGAGGTC
TTAGAAGAGATTAAGGCAGAATTGGCTGCTTGGATGATTTCAGGTGAAGATACTATTGCTTATTCAACAGAATCAAGCTA
CGCTGCCAACCTTGAGATGGCAACTAACGAATACAAACCTTCCTCAAAAGTAGCTGCTGAAGATGCCTTGGTAGAAGTTG
AGACACCACATTGCAAAACGATTGATGAAGTGGCTGCTTTTCTTTCAGTAGATGAAACACAAACCATCAAAACCTTGCTT
TTTGTGGCAGATAATGAACCTGTTGTTGCTTTGCTAGTCGGAAATGACCATATCAATACCGTTAAATTAAAAAACTATCT
AGCTGCTGATTTTTTAGAGCCAGCTAGTGAAGAAGAAGCCCGTGCTTTCTTTGGTGCAGGTTTTGGCTCACTTGGGCCTG
TCAACTTGGCACAAGGTAGTCGCATTGTGGCTGACCGCAAAGTGCAAAACCTTACCAATGCCGTTGCAGGAGCTAACAAG
GATGGTTTCCATGTGACAGGAGTCAATCCAGGACGTGACTTCCAGGCTGAATATGTAGATATTCGTGAAGTCAAAGAAGG
GGAAATGTCTCCAGATGGTCATGGTGTTCTCCAGTTTGCGCGTGGTATCGAAGTCGGTCATATCTTCAAGTTGGGCACTC
GTTATTCAGACAGCATGGGAGCAACGATTCTTGATGAAAATGGCAGAGCCGTTCCAATTGTGATGGGTTGTTATGGTATC
GGGGTTAGCCGCATTTTGTCTGCTGTCATTGAACAGCATGCCCGTCTCTTTGTGAACAAGACACCAAAAGGCGATTACCG
TTATGCTTGGGGTATTAACTTCCCTAAAGAATTAGCCCCATTTGACGTGCATTTGATTACAGTTAATGTCAAAGATCAAG
TGGCCCAAGACTTGACGGCGAAGTTAGAAGCTGACTTGATGGCTAAAGGGTATGACGTCTTGACAGATGACCGTAATGAA
CGCGTCGGTTCGAAATTCTCTGATAGCGATTTGATTGGTTTGCCAATTCGTGTCACTGTTGGTAAAAAAGCTGCTGAAGG
TATTGTGGAAATTAAAATCAAGGCAACAGGTGACAGCATTGAAGTTAATGCAGAAAACCTCATCGAAACCCTTGAAATTT
TAACAAAAGAACACTAA

Upstream 100 bases:

>100_bases
AGGAATACACATTCAAATTATTGAATAATCCCGCTTCACTCTTACTTGATGTTTCCAGAAGGGTTTGAGGATAAGGGGAT
GTATTAAAACGGAATAATCT

Downstream 100 bases:

>100_bases
AAAAGAAATGAAGAGGCTGCCTGTTTAGAGTGGCCTCTTTTTGCTGTGAACTTGTCTGATACAGAGTGGCTTTTGCTTTA
CCAAAAGCGCTTGGCGACAA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MKQSKLLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLPLANRTIEKFKTIMREEFEKIGAVEMLAPALLTADLW
RESGRYETYGEDLYKLKNRDNSDFILGPTHEETFTTLVRNAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
YSFHHNYEDLDVTYEDYRQAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAITPARTDLDRWVVLDKSIASMDDIPKEV
LEEIKAELAAWMISGEDTIAYSTESSYAANLEMATNEYKPSSKVAAEDALVEVETPHCKTIDEVAAFLSVDETQTIKTLL
FVADNEPVVALLVGNDHINTVKLKNYLAADFLEPASEEEARAFFGAGFGSLGPVNLAQGSRIVADRKVQNLTNAVAGANK
DGFHVTGVNPGRDFQAEYVDIREVKEGEMSPDGHGVLQFARGIEVGHIFKLGTRYSDSMGATILDENGRAVPIVMGCYGI
GVSRILSAVIEQHARLFVNKTPKGDYRYAWGINFPKELAPFDVHLITVNVKDQVAQDLTAKLEADLMAKGYDVLTDDRNE
RVGSKFSDSDLIGLPIRVTVGKKAAEGIVEIKIKATGDSIEVNAENLIETLEILTKEH

Sequences:

>Translated_618_residues
MKQSKLLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLPLANRTIEKFKTIMREEFEKIGAVEMLAPALLTADLW
RESGRYETYGEDLYKLKNRDNSDFILGPTHEETFTTLVRNAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
YSFHHNYEDLDVTYEDYRQAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAITPARTDLDRWVVLDKSIASMDDIPKEV
LEEIKAELAAWMISGEDTIAYSTESSYAANLEMATNEYKPSSKVAAEDALVEVETPHCKTIDEVAAFLSVDETQTIKTLL
FVADNEPVVALLVGNDHINTVKLKNYLAADFLEPASEEEARAFFGAGFGSLGPVNLAQGSRIVADRKVQNLTNAVAGANK
DGFHVTGVNPGRDFQAEYVDIREVKEGEMSPDGHGVLQFARGIEVGHIFKLGTRYSDSMGATILDENGRAVPIVMGCYGI
GVSRILSAVIEQHARLFVNKTPKGDYRYAWGINFPKELAPFDVHLITVNVKDQVAQDLTAKLEADLMAKGYDVLTDDRNE
RVGSKFSDSDLIGLPIRVTVGKKAAEGIVEIKIKATGDSIEVNAENLIETLEILTKEH
>Mature_618_residues
MKQSKLLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLPLANRTIEKFKTIMREEFEKIGAVEMLAPALLTADLW
RESGRYETYGEDLYKLKNRDNSDFILGPTHEETFTTLVRNAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
YSFHHNYEDLDVTYEDYRQAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAITPARTDLDRWVVLDKSIASMDDIPKEV
LEEIKAELAAWMISGEDTIAYSTESSYAANLEMATNEYKPSSKVAAEDALVEVETPHCKTIDEVAAFLSVDETQTIKTLL
FVADNEPVVALLVGNDHINTVKLKNYLAADFLEPASEEEARAFFGAGFGSLGPVNLAQGSRIVADRKVQNLTNAVAGANK
DGFHVTGVNPGRDFQAEYVDIREVKEGEMSPDGHGVLQFARGIEVGHIFKLGTRYSDSMGATILDENGRAVPIVMGCYGI
GVSRILSAVIEQHARLFVNKTPKGDYRYAWGINFPKELAPFDVHLITVNVKDQVAQDLTAKLEADLMAKGYDVLTDDRNE
RVGSKFSDSDLIGLPIRVTVGKKAAEGIVEIKIKATGDSIEVNAENLIETLEILTKEH

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=202, Percent_Identity=40.0990099009901, Blast_Score=161, Evalue=2e-39,
Organism=Escherichia coli, GI1786392, Length=619, Percent_Identity=42.3263327948304, Blast_Score=458, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI115532348, Length=197, Percent_Identity=36.0406091370558, Blast_Score=136, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6320931, Length=199, Percent_Identity=35.678391959799, Blast_Score=137, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24656200, Length=226, Percent_Identity=32.7433628318584, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 68683; Mature: 68683

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSKLLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLPLANRTIEKFKTIMREE
CCCCCEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHH
FEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDNSDFILGPTHEETFTTLVRN
HHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHH
AVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDGYSFHHNYEDLDVTYEDYRQA
HHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
YEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAITPARTDLDRWVVLDKSIASMDDIPKEV
HHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHH
LEEIKAELAAWMISGEDTIAYSTESSYAANLEMATNEYKPSSKVAAEDALVEVETPHCKT
HHHHHHHHHHHEECCCCEEEEECCCCCEEEEEEECCCCCCCCCHHHCCCEEEECCCCCCC
IDEVAAFLSVDETQTIKTLLFVADNEPVVALLVGNDHINTVKLKNYLAADFLEPASEEEA
HHHHHHHHCCCCHHEEEEEEEEECCCCEEEEEECCCCCCEEEHHHHHHHHHCCCCCCHHH
RAFFGAGFGSLGPVNLAQGSRIVADRKVQNLTNAVAGANKDGFHVTGVNPGRDFQAEYVD
HHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHH
IREVKEGEMSPDGHGVLQFARGIEVGHIFKLGTRYSDSMGATILDENGRAVPIVMGCYGI
HHHHCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCEEECCCCCEEEEEEEHHHC
GVSRILSAVIEQHARLFVNKTPKGDYRYAWGINFPKELAPFDVHLITVNVKDQVAQDLTA
CHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCCHHCCCCEEEEEEEECHHHHHHHHHH
KLEADLMAKGYDVLTDDRNERVGSKFSDSDLIGLPIRVTVGKKAAEGIVEIKIKATGDSI
HHHHHHHHCCCCEECCCCCHHCCCCCCCCCEEEEEEEEEECCCCCCCEEEEEEEECCCEE
EVNAENLIETLEILTKEH
EECHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKQSKLLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLPLANRTIEKFKTIMREE
CCCCCEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHH
FEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDNSDFILGPTHEETFTTLVRN
HHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHH
AVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDGYSFHHNYEDLDVTYEDYRQA
HHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
YEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAITPARTDLDRWVVLDKSIASMDDIPKEV
HHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHH
LEEIKAELAAWMISGEDTIAYSTESSYAANLEMATNEYKPSSKVAAEDALVEVETPHCKT
HHHHHHHHHHHEECCCCEEEEECCCCCEEEEEEECCCCCCCCCHHHCCCEEEECCCCCCC
IDEVAAFLSVDETQTIKTLLFVADNEPVVALLVGNDHINTVKLKNYLAADFLEPASEEEA
HHHHHHHHCCCCHHEEEEEEEEECCCCEEEEEECCCCCCEEEHHHHHHHHHCCCCCCHHH
RAFFGAGFGSLGPVNLAQGSRIVADRKVQNLTNAVAGANKDGFHVTGVNPGRDFQAEYVD
HHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHH
IREVKEGEMSPDGHGVLQFARGIEVGHIFKLGTRYSDSMGATILDENGRAVPIVMGCYGI
HHHHCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCEEECCCCCEEEEEEEHHHC
GVSRILSAVIEQHARLFVNKTPKGDYRYAWGINFPKELAPFDVHLITVNVKDQVAQDLTA
CHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCCHHCCCCEEEEEEEECHHHHHHHHHH
KLEADLMAKGYDVLTDDRNERVGSKFSDSDLIGLPIRVTVGKKAAEGIVEIKIKATGDSI
HHHHHHHHCCCCEECCCCCHHCCCCCCCCCEEEEEEEEEECCCCCCCEEEEEEEECCCEE
EVNAENLIETLEILTKEH
EECHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]