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The map label for this gene is lacE [H]

Identifier: 19746836

GI number: 19746836

Start: 1656327

End: 1658012

Strand: Reverse

Name: lacE [H]

Synonym: spyM18_1985

Alternate gene names: 19746836

Gene position: 1658012-1656327 (Counterclockwise)

Preceding gene: 19746837

Following gene: 19746835

Centisome position: 87.49

GC content: 38.61

Gene sequence:

>1686_bases
ATGAATGGATTAATTGCTCAGATTGAGAAAGGGAAACCTTTCTTCGAGAAAATTTCTCGAAACATCTATCTTCGCGCTAT
TCGTGATGGTTTTATCGCTGGTATGCCTGTTATCTTGTTTTCAAGTATCTTTATCTTGATTGCCTATGTTCCAAATGCAT
GGGGCTTCCATTGGAGTAAGGATATTGAGAATCTCTTGATGACGCCATACAACTACTCAATGGGTATTTTAGGTTTGTTT
GTGGCTGGTACGACAGCCAAAGCATTAACCGATTCAATGAACCGCTCATTGCCTAGCACTAACCAAATCAACTTTATTTC
AACCATGCTTGCTGCTATTGTAGGTTTTCTACTAATGGCAGCAAATCCAGCAAAAGACGGTGGTTTCTTAACTGGATTTA
TGGGAACAAAAGGTTTGTTAACGGCCTTCATCGCAGCTTTTATTACCGTAAATGTCTATAAAGTCTGTGTAAAAAACAAT
GTCACTATTCGCATGCCAGAAGAGGTGCCACCAAACATCTCTCAAGTTTTTAAAGATTTGATTCCATTTACGTTATCAGT
TGTACTGCTTTATGTGATTGAATTGATTGTTCATACTACTTTAGGCGTTAACGTTGCCGAGTCAATTGGTAAACTTCTTG
CACCATTGTTCCAAGCAGCAGATGGTTATGTTGGAATCACTATTATCTTCGGTGCATTTGCTCTCTTCTGGTTTGTTGGT
ATCCATGGACCATCTATTGTTGAACCAGCCATTGCAGCAATTACTTACGCAAATGCTGAAACCAACCTTCAATTACTTCA
AGCTGGTGAACATGCTGATAAGATCTTGACTTCAGGGACTCAAATGTTCATCGTTACCATGGGTGGTACTGGTGCAACAC
TTGTCGTTCCATTCATGTTCATGTGGTTAACAAAATCAAAACGTAACAAAGCCATTGGACGTGCATCAGTTGTTCCAACA
TTCTTTGGTGTTAACGAACCTATCTTGTTCGGTGCTCCACTTGTTTTGAACCCAATTTTCTTTATCCCATTTATCCTAGC
ACCAATTGTCAATGTATGGATTTTCAAATTCTTTATTGACACACTTGGAATGAACAGTTTCACTGCAAACTTACCTTGGA
CAACACCAGCTCCATTAGGTCTTATTCTTGGGACAAACTTCCAAGTGCTAGCATTCATTTTGGCAGCTTTGTTAATCGTA
GTTGATGTCATCATCTACTACCCATTCTTGAAAGTATATGATGAGCAAATTCTTGCTGAAGAAGCAGCAGGTACGAATTC
ATCAGATGTCCTCAAAGAAAAAGTAGCAGCAAACTTTGATACTAAAAAAGCTGATGCTATTCTTGAAAAATCAGCAGCTA
AAGAAACCAAAGAAATCACTGATCAAACTAATGTTCTCGTTCTTTGTGCCGGTGGTGGTACAAGTGGACTACTAGCAAAT
GCTTTGAATAAAGCAGCTAAAGAATATGGTGCACCTGTCACTGCAGCAGCAGGAAGTTATGGTGCTCACCGTGAGATTTT
ACCACAATATCAACTTGTTATCGTAGCTTCTAACTATGAAGATATGAAAGTGGAAACTGATAAACTTGATATTAAATTAG
CTAAAACAGAAGGGGCTCAATACATCAAATTGACCCGTGATGGTCAAGGAGCTCTTGATTTTGTAAAAGCACAGTTTGAA
AATTAA

Upstream 100 bases:

>100_bases
GTGTCACTTTAATGCATGGTCAAGATCATTTAATGACGACCATTTTATTACAAGATTTAATGAAACACATGATTGAACTT
TATAAGAGAGGAGCAAAATA

Downstream 100 bases:

>100_bases
GAAAACAGTTTAAATGTCGTAAAGTTCTCAAACACAAAAATTGTTTTGGGAACTTTATTTTTGAAAGATATTCAAGGAGA
AACTGAGATGACAAAAACAT

Product: PTS system, lactose-specific IIBC component

Products: NA

Alternate protein names: EIICB-Lac; EII-Lac; Lactose permease IIC component; PTS system lactose-specific EIIC component; Lactose-specific phosphotransferase enzyme IIB component; PTS system lactose-specific EIIB component [H]

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MNGLIAQIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIFILIAYVPNAWGFHWSKDIENLLMTPYNYSMGILGLF
VAGTTAKALTDSMNRSLPSTNQINFISTMLAAIVGFLLMAANPAKDGGFLTGFMGTKGLLTAFIAAFITVNVYKVCVKNN
VTIRMPEEVPPNISQVFKDLIPFTLSVVLLYVIELIVHTTLGVNVAESIGKLLAPLFQAADGYVGITIIFGAFALFWFVG
IHGPSIVEPAIAAITYANAETNLQLLQAGEHADKILTSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNKAIGRASVVPT
FFGVNEPILFGAPLVLNPIFFIPFILAPIVNVWIFKFFIDTLGMNSFTANLPWTTPAPLGLILGTNFQVLAFILAALLIV
VDVIIYYPFLKVYDEQILAEEAAGTNSSDVLKEKVAANFDTKKADAILEKSAAKETKEITDQTNVLVLCAGGGTSGLLAN
ALNKAAKEYGAPVTAAAGSYGAHREILPQYQLVIVASNYEDMKVETDKLDIKLAKTEGAQYIKLTRDGQGALDFVKAQFE
N

Sequences:

>Translated_561_residues
MNGLIAQIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIFILIAYVPNAWGFHWSKDIENLLMTPYNYSMGILGLF
VAGTTAKALTDSMNRSLPSTNQINFISTMLAAIVGFLLMAANPAKDGGFLTGFMGTKGLLTAFIAAFITVNVYKVCVKNN
VTIRMPEEVPPNISQVFKDLIPFTLSVVLLYVIELIVHTTLGVNVAESIGKLLAPLFQAADGYVGITIIFGAFALFWFVG
IHGPSIVEPAIAAITYANAETNLQLLQAGEHADKILTSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNKAIGRASVVPT
FFGVNEPILFGAPLVLNPIFFIPFILAPIVNVWIFKFFIDTLGMNSFTANLPWTTPAPLGLILGTNFQVLAFILAALLIV
VDVIIYYPFLKVYDEQILAEEAAGTNSSDVLKEKVAANFDTKKADAILEKSAAKETKEITDQTNVLVLCAGGGTSGLLAN
ALNKAAKEYGAPVTAAAGSYGAHREILPQYQLVIVASNYEDMKVETDKLDIKLAKTEGAQYIKLTRDGQGALDFVKAQFE
N
>Mature_561_residues
MNGLIAQIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIFILIAYVPNAWGFHWSKDIENLLMTPYNYSMGILGLF
VAGTTAKALTDSMNRSLPSTNQINFISTMLAAIVGFLLMAANPAKDGGFLTGFMGTKGLLTAFIAAFITVNVYKVCVKNN
VTIRMPEEVPPNISQVFKDLIPFTLSVVLLYVIELIVHTTLGVNVAESIGKLLAPLFQAADGYVGITIIFGAFALFWFVG
IHGPSIVEPAIAAITYANAETNLQLLQAGEHADKILTSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNKAIGRASVVPT
FFGVNEPILFGAPLVLNPIFFIPFILAPIVNVWIFKFFIDTLGMNSFTANLPWTTPAPLGLILGTNFQVLAFILAALLIV
VDVIIYYPFLKVYDEQILAEEAAGTNSSDVLKEKVAANFDTKKADAILEKSAAKETKEITDQTNVLVLCAGGGTSGLLAN
ALNKAAKEYGAPVTAAAGSYGAHREILPQYQLVIVASNYEDMKVETDKLDIKLAKTEGAQYIKLTRDGQGALDFVKAQFE
N

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=468, Percent_Identity=25.8547008547009, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014350
- InterPro:   IPR003501
- InterPro:   IPR013012
- InterPro:   IPR003352
- InterPro:   IPR004501
- InterPro:   IPR004801 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 60823; Mature: 60823

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS51100 PTS_EIIB_TYPE_3 ; PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGLIAQIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIFILIAYVPNAWGFHWSK
CCCCHHEECCCCHHHHHHCCCEEEEEHHCCHHCCCHHHHHHHHHHHHHHCCCCCCCCHHH
DIENLLMTPYNYSMGILGLFVAGTTAKALTDSMNRSLPSTNQINFISTMLAAIVGFLLMA
HHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHC
ANPAKDGGFLTGFMGTKGLLTAFIAAFITVNVYKVCVKNNVTIRMPEEVPPNISQVFKDL
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHH
IPFTLSVVLLYVIELIVHTTLGVNVAESIGKLLAPLFQAADGYVGITIIFGAFALFWFVG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
IHGPSIVEPAIAAITYANAETNLQLLQAGEHADKILTSGTQMFIVTMGGTGATLVVPFMF
CCCCHHHHHHHHHHEECCCCCCHHHHHCCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHH
MWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPLVLNPIFFIPFILAPIVNVWIFKFFID
HHHHHCHHHHCCCCHHCCHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLGMNSFTANLPWTTPAPLGLILGTNFQVLAFILAALLIVVDVIIYYPFLKVYDEQILAE
HHCCCCEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAAGTNSSDVLKEKVAANFDTKKADAILEKSAAKETKEITDQTNVLVLCAGGGTSGLLAN
HHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHH
ALNKAAKEYGAPVTAAAGSYGAHREILPQYQLVIVASNYEDMKVETDKLDIKLAKTEGAQ
HHHHHHHHHCCCEEECCCCCCCCHHHCCCEEEEEEECCCCCCEEECCEEEEEEEECCCCE
YIKLTRDGQGALDFVKAQFEN
EEEEECCCCCHHHHHHHHCCC
>Mature Secondary Structure
MNGLIAQIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIFILIAYVPNAWGFHWSK
CCCCHHEECCCCHHHHHHCCCEEEEEHHCCHHCCCHHHHHHHHHHHHHHCCCCCCCCHHH
DIENLLMTPYNYSMGILGLFVAGTTAKALTDSMNRSLPSTNQINFISTMLAAIVGFLLMA
HHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHC
ANPAKDGGFLTGFMGTKGLLTAFIAAFITVNVYKVCVKNNVTIRMPEEVPPNISQVFKDL
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHH
IPFTLSVVLLYVIELIVHTTLGVNVAESIGKLLAPLFQAADGYVGITIIFGAFALFWFVG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
IHGPSIVEPAIAAITYANAETNLQLLQAGEHADKILTSGTQMFIVTMGGTGATLVVPFMF
CCCCHHHHHHHHHHEECCCCCCHHHHHCCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHH
MWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPLVLNPIFFIPFILAPIVNVWIFKFFID
HHHHHCHHHHCCCCHHCCHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLGMNSFTANLPWTTPAPLGLILGTNFQVLAFILAALLIVVDVIIYYPFLKVYDEQILAE
HHCCCCEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAAGTNSSDVLKEKVAANFDTKKADAILEKSAAKETKEITDQTNVLVLCAGGGTSGLLAN
HHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHH
ALNKAAKEYGAPVTAAAGSYGAHREILPQYQLVIVASNYEDMKVETDKLDIKLAKTEGAQ
HHHHHHHHHCCCEEECCCCCCCCHHHCCCEEEEEEECCCCCCEEECCEEEEEEEECCCCE
YIKLTRDGQGALDFVKAQFEN
EEEEECCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1400164; 8277252; 12397186 [H]