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The map label for this gene is gatA [H]

Identifier: 19746723

GI number: 19746723

Start: 1520399

End: 1521865

Strand: Reverse

Name: gatA [H]

Synonym: spyM18_1840

Alternate gene names: 19746723

Gene position: 1521865-1520399 (Counterclockwise)

Preceding gene: 19746724

Following gene: 19746722

Centisome position: 80.31

GC content: 43.69

Gene sequence:

>1467_bases
ATGTCTTTTAATCATAAAACCATTGAAGAGTTGCATGACCTCCTTGTAGCTAAGGAGATTTCCGCAACCGAATTGACTCA
AAAAACACTTGAAGACATCAAGTCTCGTGAGGAGGCTGTCGGGTCGTTTATCACTGTTTCAGAAGAAGCTGCTCTTAAAC
AAGCTGCGGCAATCGATGCCAAAGGTATTGATGCTGATAACCTCATGTCGGGTATTCCACTAGCTGTTAAGGATAATATC
TCAACCAAGGGCATCCTAACCACAGCCGCTTCAAAAATGTTGTATAACTACGAGCCGATTTTTGATGCCACATCAGTAGC
AAATGCCTACGCTAAAGACATGATTGTCATTGGAAAAACCAACATGGACGAATTTGCCATGGGTGGTTCAACAGAAACCT
CTTATTTCAAGAAAACGAAAAATGCTTGGGACCACACAAAAGTACCTGGTGGCTCATCAGGTGGTTCGGCAACAGCAGTC
GCTTCAGGACAAGTCCGTTTATCTCTTGGTTCAGACACTGGTGGTTCTATTCGGCAGCCAGCTGCATTTAATGGTGTTGT
CGGTTTGAAACCAACCTATGGCACTGTTTCTCGCTATGGTCTCATTGCTTTTGGAAGTTCACTTGATCAGATTGGACCTT
TTGCTCCGACAGTTAAAGAAAACGCCCAATTGTTAAATGTGATTGCAAGTAGCGATGTCAAGGATGCCACTTCAGCACCT
GTTCGCATTGCAGACTACACGTCTAAAATTGGTCGTGATATTAAAGGCATGAAGATTGCCCTTCCTAAAGAGTATCTTGG
CGAAGGGATTGATCCTGAGATTAAGGAAACGGTTCTTGCGGCAGCTAAACAATTTGAAGCCTTAGGAGCTACTGTTGAGG
AAGTCAGCCTTCCACATAGCAAGTACGGTGTGGCTGTTTATTATATTATTGCTTCGTCAGAAGCGTCTTCAAACTTACAA
CGTTTTGATGGCATTCGCTATGGTTTCCGTGCTGACGATGCTAAAAACTTGGATGAAATCTATGTTAACACTCGTAGCCA
AGGCTTTGGTGATGAGGTGAAACGTCGTATCATGCTTGGAACCTTTAGTTTGTCATCGGGTTATTATGACGCTTATTTCA
AAAAGGCCGGTCAAGTGCGCACTTTGATTATTCAAGATTTTGACAAGGTCTTTGCGGATTATGATCTTATTCTAGGCCCA
ACAACACCGACGGTTGCTTTTGGCTTAGACACACTCAACCACGATCCTGTTGCCATGTATTTGGCCGACTTATTGACCAT
TCCGGTTAACTTGGCTGGTCTGCCAGGTATTTCTATTCCGGCAGGATTCGTCGATGGCTTGCCAGTAGGGCTTCAATTAA
TTGGTCCGAAATACGCTGAAGAAACCATCTATCAAGCAGCAGCAGCCTTTGAAGCGGTGACAGATTATCACAAGCAACAA
CCGATTATTTTTGGAGGTGACAAGTAA

Upstream 100 bases:

>100_bases
AAGAAGGAACAGACCGTGCCTTGCTCTTTAAAAATGTTCCTGAAAAAGAAAATCACTTTATCAAGGTACCTGCTATTTTG
TATGACGGAGGAGATGCCTA

Downstream 100 bases:

>100_bases
TGAATTTTGAAACCATTATTGGGCTTGAAGTCCATGTGGAATTAAATACCAATTCCAAAATTTTCTCACCTTCGTCAGCT
CACTTTGGTGAGGACCCAAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSFNHKTIEELHDLLVAKEISATELTQKTLEDIKSREEAVGSFITVSEEAALKQAAAIDAKGIDADNLMSGIPLAVKDNI
STKGILTTAASKMLYNYEPIFDATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAV
ASGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTVKENAQLLNVIASSDVKDATSAP
VRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKETVLAAAKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQ
RFDGIRYGFRADDAKNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIQDFDKVFADYDLILGP
TTPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVGLQLIGPKYAEETIYQAAAAFEAVTDYHKQQ
PIIFGGDK

Sequences:

>Translated_488_residues
MSFNHKTIEELHDLLVAKEISATELTQKTLEDIKSREEAVGSFITVSEEAALKQAAAIDAKGIDADNLMSGIPLAVKDNI
STKGILTTAASKMLYNYEPIFDATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAV
ASGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTVKENAQLLNVIASSDVKDATSAP
VRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKETVLAAAKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQ
RFDGIRYGFRADDAKNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIQDFDKVFADYDLILGP
TTPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVGLQLIGPKYAEETIYQAAAAFEAVTDYHKQQ
PIIFGGDK
>Mature_487_residues
SFNHKTIEELHDLLVAKEISATELTQKTLEDIKSREEAVGSFITVSEEAALKQAAAIDAKGIDADNLMSGIPLAVKDNIS
TKGILTTAASKMLYNYEPIFDATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAVA
SGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTVKENAQLLNVIASSDVKDATSAPV
RIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKETVLAAAKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQR
FDGIRYGFRADDAKNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIQDFDKVFADYDLILGPT
TPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVGLQLIGPKYAEETIYQAAAAFEAVTDYHKQQP
IIFGGDK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=506, Percent_Identity=37.7470355731225, Blast_Score=306, Evalue=4e-83,
Organism=Homo sapiens, GI195972892, Length=487, Percent_Identity=23.6139630390144, Blast_Score=113, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17543272, Length=444, Percent_Identity=36.7117117117117, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17537465, Length=478, Percent_Identity=22.3849372384937, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=305, Percent_Identity=25.5737704918033, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=164, Percent_Identity=31.7073170731707, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71990152, Length=191, Percent_Identity=29.3193717277487, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=415, Percent_Identity=34.2168674698795, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6319685, Length=430, Percent_Identity=29.3023255813954, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6320448, Length=259, Percent_Identity=27.027027027027, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=39.1836734693878, Blast_Score=299, Evalue=3e-81,
Organism=Drosophila melanogaster, GI24652985, Length=466, Percent_Identity=25.3218884120172, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI19922090, Length=466, Percent_Identity=25.3218884120172, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24652981, Length=466, Percent_Identity=25.3218884120172, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24652983, Length=466, Percent_Identity=25.3218884120172, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24644968, Length=510, Percent_Identity=23.7254901960784, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI161078093, Length=500, Percent_Identity=23, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648435, Length=200, Percent_Identity=32.5, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24648437, Length=200, Percent_Identity=32.5, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45550774, Length=200, Percent_Identity=32.5, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24648441, Length=130, Percent_Identity=38.4615384615385, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24648439, Length=130, Percent_Identity=38.4615384615385, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21356731, Length=331, Percent_Identity=24.773413897281, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52205; Mature: 52073

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFNHKTIEELHDLLVAKEISATELTQKTLEDIKSREEAVGSFITVSEEAALKQAAAIDA
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHCCC
KGIDADNLMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFDATSVANAYAKDMIVIGKT
CCCCHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEECC
NMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAVASGQVRLSLGSDTGGSIRQP
CHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCC
AAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTVKENAQLLNVIASSDVKDATSAP
CCCCCCEECCCCCCCHHHCCCEEECCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
VRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKETVLAAAKQFEALGATVEEVSLPHS
EEEHHHHHHHCCCCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
KYGVAVYYIIASSEASSNLQRFDGIRYGFRADDAKNLDEIYVNTRSQGFGDEVKRRIMLG
CCCEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCHHEEEECCCCCCCHHHHHHHEEE
TFSLSSGYYDAYFKKAGQVRTLIIQDFDKVFADYDLILGPTTPTVAFGLDTLNHDPVAMY
EEECCCCCHHHHHHCCCCEEEHHHHHHHHHHCCCCEEECCCCCCEEEECCCCCCCHHHHH
LADLLTIPVNLAGLPGISIPAGFVDGLPVGLQLIGPKYAEETIYQAAAAFEAVTDYHKQQ
HHHHHHHCCCCCCCCCCCCCCHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
PIIFGGDK
CEEECCCC
>Mature Secondary Structure 
SFNHKTIEELHDLLVAKEISATELTQKTLEDIKSREEAVGSFITVSEEAALKQAAAIDA
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHCCC
KGIDADNLMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFDATSVANAYAKDMIVIGKT
CCCCHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEECC
NMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAVASGQVRLSLGSDTGGSIRQP
CHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCC
AAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTVKENAQLLNVIASSDVKDATSAP
CCCCCCEECCCCCCCHHHCCCEEECCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
VRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKETVLAAAKQFEALGATVEEVSLPHS
EEEHHHHHHHCCCCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
KYGVAVYYIIASSEASSNLQRFDGIRYGFRADDAKNLDEIYVNTRSQGFGDEVKRRIMLG
CCCEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCHHEEEECCCCCCCHHHHHHHEEE
TFSLSSGYYDAYFKKAGQVRTLIIQDFDKVFADYDLILGPTTPTVAFGLDTLNHDPVAMY
EEECCCCCHHHHHHCCCCEEEHHHHHHHHHHCCCCEEECCCCCCEEEECCCCCCCHHHHH
LADLLTIPVNLAGLPGISIPAGFVDGLPVGLQLIGPKYAEETIYQAAAAFEAVTDYHKQQ
HHHHHHHCCCCCCCCCCCCCCHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
PIIFGGDK
CEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]