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The map label for this gene is flgJ [H]

Identifier: 19746642

GI number: 19746642

Start: 1454311

End: 1455516

Strand: Reverse

Name: flgJ [H]

Synonym: spyM18_1750

Alternate gene names: 19746642

Gene position: 1455516-1454311 (Counterclockwise)

Preceding gene: 19746644

Following gene: 19746641

Centisome position: 76.81

GC content: 45.52

Gene sequence:

>1206_bases
ATGGCATTTTTAGACAAAATAAAAAGCGCAGTTATCGCAGAGTGGCACACTCATAAGATTTTGCCATCTCTGACAGCTGC
TCAAGCCATTTTAGAGAGCGGGTGGGGCAAGTATGCTCCACATAATGCTTTGTTTGGTATCAAGGCAGATAGCTCTTGGA
CTGGTAAGTCATTTAATACAAAAACACAGGAAGAGTATCAACCGGGGATTGTTACGGATATTGTAGACCGTTTTAGGGCC
TACGACAGTTGGGAGGACTCTATCGCTGATCATGGCCAGTTTTTAGTCGATAATCCACGCTATAAGGCAGTTATTGGAGA
GGCTGACTACAAAAAAGCTTGTCATGCTATAAAAGATGCAGGTTATGCGACTGCGAGTGGCTATGCGGAGCTGTTGATCC
AACTGATCGAGGAGAATAACTTGCAGGAATGGGATGCCGAAGCTATAAAAAATAAGGAGGAGCAGATGATTAGTTCTCAG
TGTCGGGAAGTCATTGAATTTTTTATTAACTTAGCAAACGCCGGAGTTGGCGTGGATAAAGATAGTTTTGCGGGCTGGCA
ATGCGCAGATGTGCCTTGTTATGCAGCAAAGCACTGGTTTGGTGTTGACCTCTGGGGAAATGCCATAGACTTACTAGATA
GCGCTGCTGCCGTAGGCTGGGAAGTCCACCGCATGCCGACAGATGCAAATCCACGGACTGGAGCATTTTTTGTCCAATCA
GTGCCATGCCATCAGTTTGGACATACGGGAATTGTCATTGAGGATAGCGACGGTTATACCATGCGTACCGTTGAGCAAAA
CATCGATGGCAATCCTGATGCTTTGTATGTCGGTGCACCAGCTCGTTTTAACACTCGTGATTTTACTGGCGTGTTGGGAT
GGTTTTACCCTCCTTACCAAGGAGATATTGTCACGCAACCAGTCAGCACCGAGCCACAAAACTCTGACACAATCGTAGAG
ACACCAAAAACAGGTACTTTTACCCTAGATGTCGCAGAGATTAATATCAGACGTTGGCCAAGTCTCGCCAGCGAAGTAGT
AGGTAGCTACAAACAAGGTGATACCGTTAGCTTTGACAGTGAGGGTTACGCCAATGGTTACTACTGGATTAGCTATGTTG
GAGGCTTAGGTATGCGTAACTACCTAGCTATTGGACAGACTGATAAAGACGGGAACCGCATCAGTATTTGGGGTAAATTA
AATTAG

Upstream 100 bases:

>100_bases
CAATCACACAACTAGCCGTCATCATAGCTATCATAGTGCTATATTTTCCACTGGCCGTGATTGCTTTGATTTTGGCTCCA
TTTATAGGAGAGGATGATAG

Downstream 100 bases:

>100_bases
ATAAGACAAACGCCCTCGCTTTTTGCGGGGGCTGTTTTTTCGCAAAAAACTTCGCATTTATTGACAAAAAGTTCAAGGCG
TGTCATAATGAGGGTGAGGA

Product: hypothetical protein

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MAFLDKIKSAVIAEWHTHKILPSLTAAQAILESGWGKYAPHNALFGIKADSSWTGKSFNTKTQEEYQPGIVTDIVDRFRA
YDSWEDSIADHGQFLVDNPRYKAVIGEADYKKACHAIKDAGYATASGYAELLIQLIEENNLQEWDAEAIKNKEEQMISSQ
CREVIEFFINLANAGVGVDKDSFAGWQCADVPCYAAKHWFGVDLWGNAIDLLDSAAAVGWEVHRMPTDANPRTGAFFVQS
VPCHQFGHTGIVIEDSDGYTMRTVEQNIDGNPDALYVGAPARFNTRDFTGVLGWFYPPYQGDIVTQPVSTEPQNSDTIVE
TPKTGTFTLDVAEINIRRWPSLASEVVGSYKQGDTVSFDSEGYANGYYWISYVGGLGMRNYLAIGQTDKDGNRISIWGKL
N

Sequences:

>Translated_401_residues
MAFLDKIKSAVIAEWHTHKILPSLTAAQAILESGWGKYAPHNALFGIKADSSWTGKSFNTKTQEEYQPGIVTDIVDRFRA
YDSWEDSIADHGQFLVDNPRYKAVIGEADYKKACHAIKDAGYATASGYAELLIQLIEENNLQEWDAEAIKNKEEQMISSQ
CREVIEFFINLANAGVGVDKDSFAGWQCADVPCYAAKHWFGVDLWGNAIDLLDSAAAVGWEVHRMPTDANPRTGAFFVQS
VPCHQFGHTGIVIEDSDGYTMRTVEQNIDGNPDALYVGAPARFNTRDFTGVLGWFYPPYQGDIVTQPVSTEPQNSDTIVE
TPKTGTFTLDVAEINIRRWPSLASEVVGSYKQGDTVSFDSEGYANGYYWISYVGGLGMRNYLAIGQTDKDGNRISIWGKL
N
>Mature_400_residues
AFLDKIKSAVIAEWHTHKILPSLTAAQAILESGWGKYAPHNALFGIKADSSWTGKSFNTKTQEEYQPGIVTDIVDRFRAY
DSWEDSIADHGQFLVDNPRYKAVIGEADYKKACHAIKDAGYATASGYAELLIQLIEENNLQEWDAEAIKNKEEQMISSQC
REVIEFFINLANAGVGVDKDSFAGWQCADVPCYAAKHWFGVDLWGNAIDLLDSAAAVGWEVHRMPTDANPRTGAFFVQSV
PCHQFGHTGIVIEDSDGYTMRTVEQNIDGNPDALYVGAPARFNTRDFTGVLGWFYPPYQGDIVTQPVSTEPQNSDTIVET
PKTGTFTLDVAEINIRRWPSLASEVVGSYKQGDTVSFDSEGYANGYYWISYVGGLGMRNYLAIGQTDKDGNRISIWGKLN

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=122, Percent_Identity=37.7049180327869, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 44450; Mature: 44319

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS50911 CHAP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFLDKIKSAVIAEWHTHKILPSLTAAQAILESGWGKYAPHNALFGIKADSSWTGKSFNT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCC
KTQEEYQPGIVTDIVDRFRAYDSWEDSIADHGQFLVDNPRYKAVIGEADYKKACHAIKDA
CCHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCEEECCCCEEEEECCCHHHHHHHHHHHC
GYATASGYAELLIQLIEENNLQEWDAEAIKNKEEQMISSQCREVIEFFINLANAGVGVDK
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DSFAGWQCADVPCYAAKHWFGVDLWGNAIDLLDSAAAVGWEVHRMPTDANPRTGAFFVQS
CCCCCCEECCCCHHHHHHEEEEECCCCHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEC
VPCHQFGHTGIVIEDSDGYTMRTVEQNIDGNPDALYVGAPARFNTRDFTGVLGWFYPPYQ
CCHHHCCCCEEEEECCCCCEEEEEHHCCCCCCCEEEECCCCCCCCCCHHHHHHCCCCCCC
GDIVTQPVSTEPQNSDTIVETPKTGTFTLDVAEINIRRWPSLASEVVGSYKQGDTVSFDS
CCEEECCCCCCCCCCCCEEECCCCCEEEEEEEEECHHHCHHHHHHHHCCCCCCCEEEECC
EGYANGYYWISYVGGLGMRNYLAIGQTDKDGNRISIWGKLN
CCCCCCEEEEEECCCCCCCCEEEECCCCCCCCEEEEEEECC
>Mature Secondary Structure 
AFLDKIKSAVIAEWHTHKILPSLTAAQAILESGWGKYAPHNALFGIKADSSWTGKSFNT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCC
KTQEEYQPGIVTDIVDRFRAYDSWEDSIADHGQFLVDNPRYKAVIGEADYKKACHAIKDA
CCHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCEEECCCCEEEEECCCHHHHHHHHHHHC
GYATASGYAELLIQLIEENNLQEWDAEAIKNKEEQMISSQCREVIEFFINLANAGVGVDK
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DSFAGWQCADVPCYAAKHWFGVDLWGNAIDLLDSAAAVGWEVHRMPTDANPRTGAFFVQS
CCCCCCEECCCCHHHHHHEEEEECCCCHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEC
VPCHQFGHTGIVIEDSDGYTMRTVEQNIDGNPDALYVGAPARFNTRDFTGVLGWFYPPYQ
CCHHHCCCCEEEEECCCCCEEEEEHHCCCCCCCEEEECCCCCCCCCCHHHHHHCCCCCCC
GDIVTQPVSTEPQNSDTIVETPKTGTFTLDVAEINIRRWPSLASEVVGSYKQGDTVSFDS
CCEEECCCCCCCCCCCCEEECCCCCEEEEEEEEECHHHCHHHHHHHHCCCCCCCEEEECC
EGYANGYYWISYVGGLGMRNYLAIGQTDKDGNRISIWGKLN
CCCCCCEEEEEECCCCCCCCEEEECCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]