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The map label for this gene is nusA [H]

Identifier: 19746626

GI number: 19746626

Start: 1441305

End: 1442462

Strand: Reverse

Name: nusA [H]

Synonym: spyM18_1732

Alternate gene names: 19746626

Gene position: 1442462-1441305 (Counterclockwise)

Preceding gene: 19746627

Following gene: 19746625

Centisome position: 76.12

GC content: 40.5

Gene sequence:

>1158_bases
ATGAGCAAAGAAATGCTAGAAGCCTTCCGTATTTTGGAAGAAGAAAAACACATTGATAAAGCAGATATTATTGACGCAGT
GACAGAATCATTAAAGTCTGCCTATAAACGTCGCTATGGTCAATCAGAATCATGTGTTATTGAATTTAATGAAAAAACAG
CTGATTTTCAAGTATTCACGGTTCGTGAGGTTGTTGAAGAAGTTTTTGACAGCCGGCTTGAGATTAGTTTGAAAGATGCT
CTTGCTATCAGTTCTGCCTATGAATTGGGTGATAAGATTCGCTTTGAAGAATCTGTCAATGAATTTGGACGTGTCGCAGC
TCAATCAGCTAAGCAGACTATTATGGAAAAGATGCGCCGCCAAATGCGTGAAGTGATGTTCAATGAATACAAAGAGCATG
AAGGTGAAATTATGACAGGAACAGTTGAACGGTTTGATCAACGTTTTATTTATGTCAATCTTGGTTCCTTAGAAGCACAA
TTGTCTCACCAAGACCAGATTCCAGGTGAAACTTTCAAATCACATGACCGTATTGACGTTTATGTCTACAAAGTTGAAAA
TAATCCTAAAGGAGTTAACGTCTTTGTTAGTCGTAGCCATCCAGAATTCATCAAACGTATTATGGAGCAAGAAATTCCTG
AAGTCTTTGATGGTACTGTTGAAATCATGAGTGTCTCTCGTGAAGCAGGTGACCGTACCAAGGTTGCTGTGCGCAGTCAT
AATCCTAACGTTGATGCCATCGGAACAATTGTTGGACGTGGTGGAAGTAATATTAAAAAGGTCATCAGCAAGTTCCATCC
AAAACGTGTAGATGCTAAGACGGGGCTTGAAATTCCAGTTGAAGAAAATATTGACGTGATTCAATGGGTAGATGATCCAG
CTGAGTTCATCTACAATGCCATTGCTCCGGCTGAAGTAGACATGGTACTTTTTGACGATGAAGATCTTAAACGTGCTACG
GTAGTAGTTCCTGATAGCAAATTATCATTAGCTATCGGGCGTCGCGGCCAAAACGTTCGTTTAGCGGCACATTTGACTGG
CTACCGTATTGATATCAAATCAGCTAGCGAATATGATCGTCTTGAGGCGGAAAAAGAAGCAGCAACAGTTGTGGAAGAGC
CAGTTGTTGACGATGTTATGACTGCCGAGGAAGTGTAA

Upstream 100 bases:

>100_bases
AGCTAAACAAAAGGAATTAAGGGAGCCTTTAGGGGGCTATTGATCGTATTAATTTAAGAAAGGATTTTTGCCAAGCTTTA
CAAGCAAGGTAGATAACATT

Downstream 100 bases:

>100_bases
GAAAAGAGGTGTTTGATGTCTAAAGTCAAAAAAATACCTTTACGAAAATCACTTGTTTCTGGTGAAATTATTGCTAAACG
AGATCTTCTTAGGATTGTAA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MSKEMLEAFRILEEEKHIDKADIIDAVTESLKSAYKRRYGQSESCVIEFNEKTADFQVFTVREVVEEVFDSRLEISLKDA
LAISSAYELGDKIRFEESVNEFGRVAAQSAKQTIMEKMRRQMREVMFNEYKEHEGEIMTGTVERFDQRFIYVNLGSLEAQ
LSHQDQIPGETFKSHDRIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDGTVEIMSVSREAGDRTKVAVRSH
NPNVDAIGTIVGRGGSNIKKVISKFHPKRVDAKTGLEIPVEENIDVIQWVDDPAEFIYNAIAPAEVDMVLFDDEDLKRAT
VVVPDSKLSLAIGRRGQNVRLAAHLTGYRIDIKSASEYDRLEAEKEAATVVEEPVVDDVMTAEEV

Sequences:

>Translated_385_residues
MSKEMLEAFRILEEEKHIDKADIIDAVTESLKSAYKRRYGQSESCVIEFNEKTADFQVFTVREVVEEVFDSRLEISLKDA
LAISSAYELGDKIRFEESVNEFGRVAAQSAKQTIMEKMRRQMREVMFNEYKEHEGEIMTGTVERFDQRFIYVNLGSLEAQ
LSHQDQIPGETFKSHDRIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDGTVEIMSVSREAGDRTKVAVRSH
NPNVDAIGTIVGRGGSNIKKVISKFHPKRVDAKTGLEIPVEENIDVIQWVDDPAEFIYNAIAPAEVDMVLFDDEDLKRAT
VVVPDSKLSLAIGRRGQNVRLAAHLTGYRIDIKSASEYDRLEAEKEAATVVEEPVVDDVMTAEEV
>Mature_384_residues
SKEMLEAFRILEEEKHIDKADIIDAVTESLKSAYKRRYGQSESCVIEFNEKTADFQVFTVREVVEEVFDSRLEISLKDAL
AISSAYELGDKIRFEESVNEFGRVAAQSAKQTIMEKMRRQMREVMFNEYKEHEGEIMTGTVERFDQRFIYVNLGSLEAQL
SHQDQIPGETFKSHDRIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDGTVEIMSVSREAGDRTKVAVRSHN
PNVDAIGTIVGRGGSNIKKVISKFHPKRVDAKTGLEIPVEENIDVIQWVDDPAEFIYNAIAPAEVDMVLFDDEDLKRATV
VVPDSKLSLAIGRRGQNVRLAAHLTGYRIDIKSASEYDRLEAEKEAATVVEEPVVDDVMTAEEV

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=369, Percent_Identity=33.3333333333333, Blast_Score=216, Evalue=3e-57,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 43776; Mature: 43645

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEMLEAFRILEEEKHIDKADIIDAVTESLKSAYKRRYGQSESCVIEFNEKTADFQVFT
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEE
VREVVEEVFDSRLEISLKDALAISSAYELGDKIRFEESVNEFGRVAAQSAKQTIMEKMRR
HHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
QMREVMFNEYKEHEGEIMTGTVERFDQRFIYVNLGSLEAQLSHQDQIPGETFKSHDRIDV
HHHHHHHHHHHHHCCCEEEHHHHHHCCEEEEEEECCHHHHHCCCCCCCCCHHCCCCCEEE
YVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDGTVEIMSVSREAGDRTKVAVRSH
EEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECC
NPNVDAIGTIVGRGGSNIKKVISKFHPKRVDAKTGLEIPVEENIDVIQWVDDPAEFIYNA
CCCCCCHHHEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHH
IAPAEVDMVLFDDEDLKRATVVVPDSKLSLAIGRRGQNVRLAAHLTGYRIDIKSASEYDR
CCCCEEEEEEECCCCCCEEEEEECCCCEEEEECCCCCCEEEEEEEEEEEEEECCCCHHHH
LEAEKEAATVVEEPVVDDVMTAEEV
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKEMLEAFRILEEEKHIDKADIIDAVTESLKSAYKRRYGQSESCVIEFNEKTADFQVFT
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEE
VREVVEEVFDSRLEISLKDALAISSAYELGDKIRFEESVNEFGRVAAQSAKQTIMEKMRR
HHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
QMREVMFNEYKEHEGEIMTGTVERFDQRFIYVNLGSLEAQLSHQDQIPGETFKSHDRIDV
HHHHHHHHHHHHHCCCEEEHHHHHHCCEEEEEEECCHHHHHCCCCCCCCCHHCCCCCEEE
YVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDGTVEIMSVSREAGDRTKVAVRSH
EEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECC
NPNVDAIGTIVGRGGSNIKKVISKFHPKRVDAKTGLEIPVEENIDVIQWVDDPAEFIYNA
CCCCCCHHHEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHH
IAPAEVDMVLFDDEDLKRATVVVPDSKLSLAIGRRGQNVRLAAHLTGYRIDIKSASEYDR
CCCCEEEEEEECCCCCCEEEEEECCCCEEEEECCCCCCEEEEEEEEEEEEEECCCCHHHH
LEAEKEAATVVEEPVVDDVMTAEEV
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8491709; 9384377 [H]