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The map label for this gene is gatC [H]

Identifier: 19746614

GI number: 19746614

Start: 1429628

End: 1431076

Strand: Reverse

Name: gatC [H]

Synonym: spyM18_1719

Alternate gene names: 19746614

Gene position: 1431076-1429628 (Counterclockwise)

Preceding gene: 19746615

Following gene: 19746613

Centisome position: 75.52

GC content: 41.48

Gene sequence:

>1449_bases
ATGCAACCTTTTTTAGACATTATTAACAAGATTCTGGGATTTCCTATTCAGTTGGGATCAGGTGTGGTCATGCTGATTGT
CATGACAGGTTTAGCCATGATTTTCGGGGTTAAATTCACAAAGGCACTGGAAGGCGGGATTAAGTTAGCTATCGCTCTTA
CAGGTATTGGAGCCATTATTGGGATTTTGACGGGGGCTTTTTCAGAGTCTTTGCAGGCCTTTGTCAAAAATACAGGAATC
AGTTTGAATATTATTGATGTCGGTTGGGCACCGCTTGCAACAATTACCTGGGGTTCCCCTTATACCCTTTATTTCTTGCT
GGTGATGGTGGTGGTTAATATTGTCATGATTGTCATGAAAAAGACAGACACCCTTGACGTCGATATTTTTGATATTTGGC
ACTTATCCATTACGGGCTTGTTAATTATGTGGTATGCGGCTCGCAACCATTTACCAGTCTTTGTGTCTCTTTTGATTGCT
ACTGTGGCAGTTATTCTTGTTGGGGTACTGAAAATCATTAACTCTGATTTGATGAAACCCACCTTTGATGATCTCCTTGG
AACAGGGCCTCAATCACCGATGACATCGACCCATATGAACTACATGATGAATCCAATCATTATGGTTTTAGATAAGATTT
TTGACAAGGTCTTTCCTGGGCTAGATAAATATGACTTTGATGCTGCTAAATTGAACAAAAAAATTGGCTTCTGGGGCTCT
AAGTTCTTTATCGGAATGGCTCTAGGTTTTGTGATTGGGATTATGGGAGATCCTCATTTCACTGTTGAATCTATCAAAAA
TTGGTTTGGTCTAGGATTTACAGCTGGTGCCTGCTTGGAACTTTTCTCTTTGATTGGTTCTTGGTTTATCGCAGCTGTTG
AGCCTTTGTCACAAGGGATTACTAATTTTGCCAATGCTAGAATGCATGGACGTCGTTTCAATATCGGTTTGGACTGGCCT
TTCATTGCTGGTCGCGCTGAAATCTGGGCCTGTGCCAATATTCTTGCCCCAATCATGTTGATTGAAGCCGTTCTCTTATC
AAAAGTTGGCAATGGGATTTTACCATTGGCGGGAATTATTGCTATGGGGATGACACCAGCTCTTCTTGTGGTCACACGTG
GTCGTTTGATTCGCATGATTATTTTTGGTTCCCTCTTATTGCCATTATTCCTCTTGTCAGGAACTATGATTGCTCCATTT
GCGACAGAGTTGGCTAAAAAAGTAGGGGCTTTTCCAGCAGGTACAAGCGCTGGTTCCTTGATTACGCATTCAACACTTGA
AGGACCAATGGAAAAAATTTTTGGTTATGTGATTGGTCAAGCGACAACAGGTCAGATTGCCTCAATCATCACGCTAATCA
TCTTTGTGGCCATTTACCTAGGTCTCTTTGCTTGGTATGCTAACCAAATGAAGGCACGTAACGCTGAGTATGCGAAAACA
ATGAAATAA

Upstream 100 bases:

>100_bases
CGTATCCCAATCATGAGTGAGCCTGTTTTTGCTGAATTAGAGCGCGTGATTAAAGAAAACCATTTGCAGTGAGTTAATAA
AAACATAAGGAGACAAGATG

Downstream 100 bases:

>100_bases
AATCAGGTTAGGTGACTAACTAAAAAACTATTGGGAATAGCAGACAACCGCTTAAGGTATTCCTGCCTTATCCCCTAGGG
AGAAAAGAGCATTCCTCATG

Product: PTS system, galactose-specific IIC component

Products: galactitol-1-phosphate [Cytoplasm]; pyruvate [C]

Alternate protein names: EIIC-Gat; PTS system galactitol-specific EIIC component [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MQPFLDIINKILGFPIQLGSGVVMLIVMTGLAMIFGVKFTKALEGGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGI
SLNIIDVGWAPLATITWGSPYTLYFLLVMVVVNIVMIVMKKTDTLDVDIFDIWHLSITGLLIMWYAARNHLPVFVSLLIA
TVAVILVGVLKIINSDLMKPTFDDLLGTGPQSPMTSTHMNYMMNPIIMVLDKIFDKVFPGLDKYDFDAAKLNKKIGFWGS
KFFIGMALGFVIGIMGDPHFTVESIKNWFGLGFTAGACLELFSLIGSWFIAAVEPLSQGITNFANARMHGRRFNIGLDWP
FIAGRAEIWACANILAPIMLIEAVLLSKVGNGILPLAGIIAMGMTPALLVVTRGRLIRMIIFGSLLLPLFLLSGTMIAPF
ATELAKKVGAFPAGTSAGSLITHSTLEGPMEKIFGYVIGQATTGQIASIITLIIFVAIYLGLFAWYANQMKARNAEYAKT
MK

Sequences:

>Translated_482_residues
MQPFLDIINKILGFPIQLGSGVVMLIVMTGLAMIFGVKFTKALEGGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGI
SLNIIDVGWAPLATITWGSPYTLYFLLVMVVVNIVMIVMKKTDTLDVDIFDIWHLSITGLLIMWYAARNHLPVFVSLLIA
TVAVILVGVLKIINSDLMKPTFDDLLGTGPQSPMTSTHMNYMMNPIIMVLDKIFDKVFPGLDKYDFDAAKLNKKIGFWGS
KFFIGMALGFVIGIMGDPHFTVESIKNWFGLGFTAGACLELFSLIGSWFIAAVEPLSQGITNFANARMHGRRFNIGLDWP
FIAGRAEIWACANILAPIMLIEAVLLSKVGNGILPLAGIIAMGMTPALLVVTRGRLIRMIIFGSLLLPLFLLSGTMIAPF
ATELAKKVGAFPAGTSAGSLITHSTLEGPMEKIFGYVIGQATTGQIASIITLIIFVAIYLGLFAWYANQMKARNAEYAKT
MK
>Mature_482_residues
MQPFLDIINKILGFPIQLGSGVVMLIVMTGLAMIFGVKFTKALEGGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGI
SLNIIDVGWAPLATITWGSPYTLYFLLVMVVVNIVMIVMKKTDTLDVDIFDIWHLSITGLLIMWYAARNHLPVFVSLLIA
TVAVILVGVLKIINSDLMKPTFDDLLGTGPQSPMTSTHMNYMMNPIIMVLDKIFDKVFPGLDKYDFDAAKLNKKIGFWGS
KFFIGMALGFVIGIMGDPHFTVESIKNWFGLGFTAGACLELFSLIGSWFIAAVEPLSQGITNFANARMHGRRFNIGLDWP
FIAGRAEIWACANILAPIMLIEAVLLSKVGNGILPLAGIIAMGMTPALLVVTRGRLIRMIIFGSLLLPLFLLSGTMIAPF
ATELAKKVGAFPAGTSAGSLITHSTLEGPMEKIFGYVIGQATTGQIASIITLIIFVAIYLGLFAWYANQMKARNAEYAKT
MK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3775

COG function: function code G; Phosphotransferase system, galactitol-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788408, Length=413, Percent_Identity=25.4237288135593, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1790758, Length=407, Percent_Identity=28.009828009828, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013853
- InterPro:   IPR013014
- InterPro:   IPR004703 [H]

Pfam domain/function: PF03611 EIIC-GAT [H]

EC number: NA

Molecular weight: Translated: 52218; Mature: 52218

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPFLDIINKILGFPIQLGSGVVMLIVMTGLAMIFGVKFTKALEGGIKLAIALTGIGAII
CCHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHH
GILTGAFSESLQAFVKNTGISLNIIDVGWAPLATITWGSPYTLYFLLVMVVVNIVMIVMK
HHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
KTDTLDVDIFDIWHLSITGLLIMWYAARNHLPVFVSLLIATVAVILVGVLKIINSDLMKP
CCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TFDDLLGTGPQSPMTSTHMNYMMNPIIMVLDKIFDKVFPGLDKYDFDAAKLNKKIGFWGS
CHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHH
KFFIGMALGFVIGIMGDPHFTVESIKNWFGLGFTAGACLELFSLIGSWFIAAVEPLSQGI
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TNFANARMHGRRFNIGLDWPFIAGRAEIWACANILAPIMLIEAVLLSKVGNGILPLAGII
HHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AMGMTPALLVVTRGRLIRMIIFGSLLLPLFLLSGTMIAPFATELAKKVGAFPAGTSAGSL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCH
ITHSTLEGPMEKIFGYVIGQATTGQIASIITLIIFVAIYLGLFAWYANQMKARNAEYAKT
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHC
MK
CC
>Mature Secondary Structure
MQPFLDIINKILGFPIQLGSGVVMLIVMTGLAMIFGVKFTKALEGGIKLAIALTGIGAII
CCHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHH
GILTGAFSESLQAFVKNTGISLNIIDVGWAPLATITWGSPYTLYFLLVMVVVNIVMIVMK
HHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
KTDTLDVDIFDIWHLSITGLLIMWYAARNHLPVFVSLLIATVAVILVGVLKIINSDLMKP
CCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TFDDLLGTGPQSPMTSTHMNYMMNPIIMVLDKIFDKVFPGLDKYDFDAAKLNKKIGFWGS
CHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHH
KFFIGMALGFVIGIMGDPHFTVESIKNWFGLGFTAGACLELFSLIGSWFIAAVEPLSQGI
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TNFANARMHGRRFNIGLDWPFIAGRAEIWACANILAPIMLIEAVLLSKVGNGILPLAGII
HHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AMGMTPALLVVTRGRLIRMIIFGSLLLPLFLLSGTMIAPFATELAKKVGAFPAGTSAGSL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCH
ITHSTLEGPMEKIFGYVIGQATTGQIASIITLIIFVAIYLGLFAWYANQMKARNAEYAKT
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHC
MK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoenolpyruvate; galactitol [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + galactitol [Periplasm] = galactitol-1-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]