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The map label for this gene is mraW [H]

Identifier: 19746583

GI number: 19746583

Start: 1396539

End: 1397552

Strand: Reverse

Name: mraW [H]

Synonym: spyM18_1676

Alternate gene names: 19746583

Gene position: 1397552-1396539 (Counterclockwise)

Preceding gene: 19746584

Following gene: 19746582

Centisome position: 73.75

GC content: 39.74

Gene sequence:

>1014_bases
ATGAAATCCTTTTCTCTTACTTTTTCATTTCTAAACCTTTTGAAGTATGGTACAATTAAAGTTATGACAAAAGAATTTCA
TCACGTGACCGTACTCCTTCACGAAACAGTGGACATGCTTGACATAAAGCCTGATGGGATTTATGTTGATGCGACGCTAG
GTGGCTCAGGCCACTCAGCTTATTTGTTGTCCAAACTTGGTGAAGAAGGGCACCTCTATTGTTTTGACCAAGACCAAAAG
GCTATTGACAATGCACAAGTTACCCTCAAATCTTATATTGACAAAGGACAGGTAACTTTTATTAAAGATAATTTTAGACA
CCTCAAAGCACGTTTAACAGCGCTTGGAGTTGATGAAATTGATGGTATCTTATACGACCTTGGTGTTTCCAGCCCGCAAT
TGGATGAAAGAGAACGAGGGTTTTCTTATAAACAAGATGCTCCATTGGATATGCGCATGGATCGTCAGTCGCTCTTAACA
GCTTACGAAGTGGTGAATACCTATCCATTCAATGATTTGGTTAAGATTTTCTTCAAATATGGTGAAGATAAATTCTCCAA
GCAGATTGCTCGAAAAATTGAACAAGCAAGAGCTATTAAGCCTATTGAGACAACAACAGAGTTGGCAGAATTGATTAAGG
CAGCAAAGCCAGCTAAAGAGTTGAAGAAAAAAGGCCACCCTGCTAAACAGATTTTTCAAGCTATTCGCATTGAAGTCAAT
GATGAATTGGGAGCGGCCGATGAATCTATTCAGGACGCTATGGAATTATTAGCCCTTGATGGTCGTATCTCAGTCATTAC
CTTCCATTCTCTGGAAGATCGCCTAACCAAGCAGTTGTTTAAAGAAGCTAGTACGGTGGATGTGCCAAAAGGGCTTCCTC
TAATTCCTGAAGATATGAAACCTAAGTTTGAACTTGTTTCACGTAAGCCGATCTTACCTAGTCATTCAGAGTTAACAGCT
AATAAAAGGGCACACTCAGCCAAGCTGCGTGTTGCCAAAAAAATTCGGAAATAA

Upstream 100 bases:

>100_bases
CAGTTGAAATTCTAGAGGCAGCGGCTAAGTCAGTGCTTGCTAAGTTGGCTGATAGCGAACAAAAAGAACAAGATTAGAGA
AGAGGAAGATTCCCACGAGA

Downstream 100 bases:

>100_bases
GCCTATGACAAATGAAAAAAGAACTCAGGCAGTTACAAATGCCTTGCAAAAACGAATAAAAACTTTCTCTAGAATTGAGA
AGGCTTTTTATACTGCTATC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKSFSLTFSFLNLLKYGTIKVMTKEFHHVTVLLHETVDMLDIKPDGIYVDATLGGSGHSAYLLSKLGEEGHLYCFDQDQK
AIDNAQVTLKSYIDKGQVTFIKDNFRHLKARLTALGVDEIDGILYDLGVSSPQLDERERGFSYKQDAPLDMRMDRQSLLT
AYEVVNTYPFNDLVKIFFKYGEDKFSKQIARKIEQARAIKPIETTTELAELIKAAKPAKELKKKGHPAKQIFQAIRIEVN
DELGAADESIQDAMELLALDGRISVITFHSLEDRLTKQLFKEASTVDVPKGLPLIPEDMKPKFELVSRKPILPSHSELTA
NKRAHSAKLRVAKKIRK

Sequences:

>Translated_337_residues
MKSFSLTFSFLNLLKYGTIKVMTKEFHHVTVLLHETVDMLDIKPDGIYVDATLGGSGHSAYLLSKLGEEGHLYCFDQDQK
AIDNAQVTLKSYIDKGQVTFIKDNFRHLKARLTALGVDEIDGILYDLGVSSPQLDERERGFSYKQDAPLDMRMDRQSLLT
AYEVVNTYPFNDLVKIFFKYGEDKFSKQIARKIEQARAIKPIETTTELAELIKAAKPAKELKKKGHPAKQIFQAIRIEVN
DELGAADESIQDAMELLALDGRISVITFHSLEDRLTKQLFKEASTVDVPKGLPLIPEDMKPKFELVSRKPILPSHSELTA
NKRAHSAKLRVAKKIRK
>Mature_337_residues
MKSFSLTFSFLNLLKYGTIKVMTKEFHHVTVLLHETVDMLDIKPDGIYVDATLGGSGHSAYLLSKLGEEGHLYCFDQDQK
AIDNAQVTLKSYIDKGQVTFIKDNFRHLKARLTALGVDEIDGILYDLGVSSPQLDERERGFSYKQDAPLDMRMDRQSLLT
AYEVVNTYPFNDLVKIFFKYGEDKFSKQIARKIEQARAIKPIETTTELAELIKAAKPAKELKKKGHPAKQIFQAIRIEVN
DELGAADESIQDAMELLALDGRISVITFHSLEDRLTKQLFKEASTVDVPKGLPLIPEDMKPKFELVSRKPILPSHSELTA
NKRAHSAKLRVAKKIRK

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=340, Percent_Identity=34.4117647058824, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI165377202, Length=190, Percent_Identity=38.9473684210526, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1786270, Length=324, Percent_Identity=42.5925925925926, Blast_Score=245, Evalue=2e-66,
Organism=Drosophila melanogaster, GI62472493, Length=346, Percent_Identity=36.9942196531792, Blast_Score=177, Evalue=7e-45,
Organism=Drosophila melanogaster, GI28571637, Length=346, Percent_Identity=36.9942196531792, Blast_Score=177, Evalue=7e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 38109; Mature: 38109

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSFSLTFSFLNLLKYGTIKVMTKEFHHVTVLLHETVDMLDIKPDGIYVDATLGGSGHSA
CCCHHHHHHHHHHHHHCHHEEEHHHHHHEEEEEECCHHHEEECCCCEEEEEEECCCCCHH
YLLSKLGEEGHLYCFDQDQKAIDNAQVTLKSYIDKGQVTFIKDNFRHLKARLTALGVDEI
HHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCHHHH
DGILYDLGVSSPQLDERERGFSYKQDAPLDMRMDRQSLLTAYEVVNTYPFNDLVKIFFKY
HHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
GEDKFSKQIARKIEQARAIKPIETTTELAELIKAAKPAKELKKKGHPAKQIFQAIRIEVN
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHEEC
DELGAADESIQDAMELLALDGRISVITFHSLEDRLTKQLFKEASTVDVPKGLPLIPEDMK
CCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PKFELVSRKPILPSHSELTANKRAHSAKLRVAKKIRK
CHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSFSLTFSFLNLLKYGTIKVMTKEFHHVTVLLHETVDMLDIKPDGIYVDATLGGSGHSA
CCCHHHHHHHHHHHHHCHHEEEHHHHHHEEEEEECCHHHEEECCCCEEEEEEECCCCCHH
YLLSKLGEEGHLYCFDQDQKAIDNAQVTLKSYIDKGQVTFIKDNFRHLKARLTALGVDEI
HHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCHHHH
DGILYDLGVSSPQLDERERGFSYKQDAPLDMRMDRQSLLTAYEVVNTYPFNDLVKIFFKY
HHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
GEDKFSKQIARKIEQARAIKPIETTTELAELIKAAKPAKELKKKGHPAKQIFQAIRIEVN
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHEEC
DELGAADESIQDAMELLALDGRISVITFHSLEDRLTKQLFKEASTVDVPKGLPLIPEDMK
CCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PKFELVSRKPILPSHSELTANKRAHSAKLRVAKKIRK
CHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA