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The map label for this gene is nadE [H]

Identifier: 19746572

GI number: 19746572

Start: 1384390

End: 1385214

Strand: Reverse

Name: nadE [H]

Synonym: spyM18_1663

Alternate gene names: 19746572

Gene position: 1385214-1384390 (Counterclockwise)

Preceding gene: 19746573

Following gene: 19746571

Centisome position: 73.1

GC content: 41.82

Gene sequence:

>825_bases
ATGACATTACAAGAAGAGATTATCCGACAATTAGGCGTCAAGGCTAGCATTGATCCGAAAGAAGAAATTCGGAAAACCGT
TGATTTTTTAAAAACTTATCTGCGTAAGCACTCATTTTTGAAAACTTATGTCTTAGGCATTTCTGGAGGTCAGGATTCTA
CCCTCGCAGGAAAGCTAGCCCAGATGGCCATTGCAGAGTTACGGGAGGAAACAGGTGATCAAGCTTACCAATTTATAGCT
GTTCGCTTGCCTTATGGGGTTCAGGCTGATGAAGCAGATGCGCAAAAGGCCTTGGCTTTTATCATGCCAGACCAAACACT
GACTATTAATATTAAAGCAGCTGTTGATGGCCAAGTAGCAGCCTTGCAAGAAGCTGGCATCGAGATTTCTGATTTCAATA
AAGGTAATATTAAAGCACGTCAGCGAATGATAAGTCAATATGCCATTGCTGGCCAAATGGCTGGTGCTGTTATTGGAACG
GATCATGCGGCAGAAAACATCACTGGTTTCTTTACTAAATTTGGTGATGGTGGAGCAGATATTCTTCCCCTCTTTAGGCT
TAACAAACGTCAAGGAAAGGCTCTTTTGAAAGTTTTAGGAGCAGATGCTGCTTTGTATGAAAAAGTTCCAACTGCTGATT
TAGAAGATCAAAAACCAGGCTTGGCTGATGAGGTTGCTCTAGGGGTGACCTATCAAGACATCGACGACTATTTGGAAGGG
AAGCTGATTTCCAAAGTAGCTCAAGCAACTATTGAAAAATGGTGGCATAAAGGACAACACAAGCGTCACCTGCCAATCAC
CATTTTTGATGATTTTTGGAAATAA

Upstream 100 bases:

>100_bases
CTCAAGACTACCCAGTAGACTTAGCCCGTGATGTGTGGCAAAACAAGATGGCCTTAATTGATAACATTCGCAAAGATGCT
TATGGAAAGAGTGATTTAAA

Downstream 100 bases:

>100_bases
GAGATGATGTTCAAAGAGTAAAAAAAGAAAACAAAAAAAGTTAGGTTATCCTAACTTTTTTCTTGAAGAAAATCAAATAA
GGTCTATAATAGAAGTAATC

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTLQEEIIRQLGVKASIDPKEEIRKTVDFLKTYLRKHSFLKTYVLGISGGQDSTLAGKLAQMAIAELREETGDQAYQFIA
VRLPYGVQADEADAQKALAFIMPDQTLTINIKAAVDGQVAALQEAGIEISDFNKGNIKARQRMISQYAIAGQMAGAVIGT
DHAAENITGFFTKFGDGGADILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQKPGLADEVALGVTYQDIDDYLEG
KLISKVAQATIEKWWHKGQHKRHLPITIFDDFWK

Sequences:

>Translated_274_residues
MTLQEEIIRQLGVKASIDPKEEIRKTVDFLKTYLRKHSFLKTYVLGISGGQDSTLAGKLAQMAIAELREETGDQAYQFIA
VRLPYGVQADEADAQKALAFIMPDQTLTINIKAAVDGQVAALQEAGIEISDFNKGNIKARQRMISQYAIAGQMAGAVIGT
DHAAENITGFFTKFGDGGADILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQKPGLADEVALGVTYQDIDDYLEG
KLISKVAQATIEKWWHKGQHKRHLPITIFDDFWK
>Mature_273_residues
TLQEEIIRQLGVKASIDPKEEIRKTVDFLKTYLRKHSFLKTYVLGISGGQDSTLAGKLAQMAIAELREETGDQAYQFIAV
RLPYGVQADEADAQKALAFIMPDQTLTINIKAAVDGQVAALQEAGIEISDFNKGNIKARQRMISQYAIAGQMAGAVIGTD
HAAENITGFFTKFGDGGADILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQKPGLADEVALGVTYQDIDDYLEGK
LISKVAQATIEKWWHKGQHKRHLPITIFDDFWK

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=274, Percent_Identity=66.4233576642336, Blast_Score=380, Evalue=1e-107,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 30209; Mature: 30078

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQEEIIRQLGVKASIDPKEEIRKTVDFLKTYLRKHSFLKTYVLGISGGQDSTLAGKLA
CCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHH
QMAIAELREETGDQAYQFIAVRLPYGVQADEADAQKALAFIMPDQTLTINIKAAVDGQVA
HHHHHHHHHHCCCHHEEEEEEECCCCCCCCHHHHHHHEEEEECCCEEEEEEEEECCCHHH
ALQEAGIEISDFNKGNIKARQRMISQYAIAGQMAGAVIGTDHAAENITGFFTKFGDGGAD
HHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHCCCCCCC
ILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQKPGLADEVALGVTYQDIDDYLEG
CHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHEECCCHHHHHHHHHH
KLISKVAQATIEKWWHKGQHKRHLPITIFDDFWK
HHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCC
>Mature Secondary Structure 
TLQEEIIRQLGVKASIDPKEEIRKTVDFLKTYLRKHSFLKTYVLGISGGQDSTLAGKLA
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHH
QMAIAELREETGDQAYQFIAVRLPYGVQADEADAQKALAFIMPDQTLTINIKAAVDGQVA
HHHHHHHHHHCCCHHEEEEEEECCCCCCCCHHHHHHHEEEEECCCEEEEEEEEECCCHHH
ALQEAGIEISDFNKGNIKARQRMISQYAIAGQMAGAVIGTDHAAENITGFFTKFGDGGAD
HHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHCCCCCCC
ILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQKPGLADEVALGVTYQDIDDYLEG
CHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHEECCCHHHHHHHHHH
KLISKVAQATIEKWWHKGQHKRHLPITIFDDFWK
HHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]