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The map label for this gene is gmuD [H]

Identifier: 19746527

GI number: 19746527

Start: 1328976

End: 1330418

Strand: Reverse

Name: gmuD [H]

Synonym: spyM18_1605

Alternate gene names: 19746527

Gene position: 1330418-1328976 (Counterclockwise)

Preceding gene: 19746528

Following gene: 19746525

Centisome position: 70.21

GC content: 44.7

Gene sequence:

>1443_bases
ATGTTAGCATTTCCAAAAGAGTTTTGGTGGGGAGGAGCCACCTCAGGCCCTCAAAGTGAGGGGCGTTTTGCTAAGCAGCA
CCGCAATCTCTTTGATTATTGGTATGAAGAGGAGCCTGACTTGTTTTACGATTACGTAGGACCTGATACGGCTTCGGATG
CCTATCATCAGATTGAATCTGATTTGGCCTTGTTAGCTTCACTAGGTCACAATTCTTACCGCACCTCCATTCAGTGGACA
CGTTTGATTGATGACTTTGAGCAAGCGACGATTAATCCTGATGGCCTTGCTTACTATAACCGAGTGATCAATGCTTGCTT
GGCCAATGGTATTCGTCCTGTTATTAATTTGCATCATTTTGACCTGCCAATTGCCCTCTATCAAGCCTATGGCGGCTGGG
AATCAAAGCATGTCGTGGACATATTTGTCGCCTTTAGCAAGGTTTGTTTTGAGCAGTTTGGTGATCGGGTTAAGGATTGG
TTTGTCCACAATGAACCTATGGTCGTTGTGGAAGGCTCTTATCTAATGCAATTTCATTACCCTGCTATCGTGGATGGGAA
AAAAGCAGTGCAGGTCGCCTATAATCTAGCCCTAGCCACAGCCAAGGTTATTCAAGCTTACAGGCGAGGTCCAGCAGAGC
TTAGTAATGGTCGTATTGGAACTATTCTCAATCTGACGCCAGCCTACCCAGCTAGTCAGTCAGAAGCGGACATGGCTGCT
GCCCATTTTACGGAGCTTTGGAACAATGACTTGTTTATGGAAGCTGCCGTTCATGGGAAATTCCCAGAAGAATTGGTTGC
GGTATTGAAAAAAGATGGGGTTTTGTGGCAATCAACGCCAGAAGAATTGGCTCTTATTGCTGAGAATAGAGTGGATTATT
TGGGATTGAATTTTTACCATCCTAAGCGCGTGAAGGCTCCGGATGTCATTCCAGTGATTAGCCCATCTTGGAGTCCTGAA
TGGTATTATGATCCCTACCTGATGCCTGGCCGCCGCATGAACGTGGATAAGGGGTGGGAGATCTATCCTGAGGCTGTCTA
TGATATTGCCATCAAGATGCGTGACCATTACGACAATATTCCTTGGTTCTTATCTGAAAATGGTGTAGGGATTTCAGGAG
AAGACCGTTACCGTGATGAAACTGGTCAGATTCAAGACGATTATCGCATCCAATTTTTGAAAGAACATTTGACCTATCTC
CACAAAGGAATAGAAGCAGGCTCGAATTGTTTTGGTTACCATGTATGGACACCAATTGATGGCTGGTCTTGGCTCAATGC
CTATAAAAACCGCTATGGCCTTGTGGAAAATAATATCCATACCCAAGTGCGACGACCTAAAGCTTCAGCTTATTGGTTTA
AAAAAGTAGCTACTCATAACTGCTTGATTTCATTAGAAGTAATGGAGGAGTTTGGAGGCTCTGCTTCTCACCTCAGTGAT
TGA

Upstream 100 bases:

>100_bases
TCTACTATCAACAGCAAGATAAACTAACAGCTAATCAAGGTCAAGTTACCCAAGCAAGACCGGAAGACTATGCTTAGCAT
ACGAGCTAGAAAGGAATGAT

Downstream 100 bases:

>100_bases
ACGTGTGAATGTCTCCTTTGAGACAAGTGAATAATGATGTTACCAATGATTAAACAAGGGCCTTTTTCCTCATGTTAAGA
GGATCGAGGCTCTTGTATTT

Product: beta-glucosidase

Products: NA

Alternate protein names: Aryl-phospho-beta-D-glucosidase BglD; Glucomannan utilization protein D [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MLAFPKEFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPDLFYDYVGPDTASDAYHQIESDLALLASLGHNSYRTSIQWT
RLIDDFEQATINPDGLAYYNRVINACLANGIRPVINLHHFDLPIALYQAYGGWESKHVVDIFVAFSKVCFEQFGDRVKDW
FVHNEPMVVVEGSYLMQFHYPAIVDGKKAVQVAYNLALATAKVIQAYRRGPAELSNGRIGTILNLTPAYPASQSEADMAA
AHFTELWNNDLFMEAAVHGKFPEELVAVLKKDGVLWQSTPEELALIAENRVDYLGLNFYHPKRVKAPDVIPVISPSWSPE
WYYDPYLMPGRRMNVDKGWEIYPEAVYDIAIKMRDHYDNIPWFLSENGVGISGEDRYRDETGQIQDDYRIQFLKEHLTYL
HKGIEAGSNCFGYHVWTPIDGWSWLNAYKNRYGLVENNIHTQVRRPKASAYWFKKVATHNCLISLEVMEEFGGSASHLSD

Sequences:

>Translated_480_residues
MLAFPKEFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPDLFYDYVGPDTASDAYHQIESDLALLASLGHNSYRTSIQWT
RLIDDFEQATINPDGLAYYNRVINACLANGIRPVINLHHFDLPIALYQAYGGWESKHVVDIFVAFSKVCFEQFGDRVKDW
FVHNEPMVVVEGSYLMQFHYPAIVDGKKAVQVAYNLALATAKVIQAYRRGPAELSNGRIGTILNLTPAYPASQSEADMAA
AHFTELWNNDLFMEAAVHGKFPEELVAVLKKDGVLWQSTPEELALIAENRVDYLGLNFYHPKRVKAPDVIPVISPSWSPE
WYYDPYLMPGRRMNVDKGWEIYPEAVYDIAIKMRDHYDNIPWFLSENGVGISGEDRYRDETGQIQDDYRIQFLKEHLTYL
HKGIEAGSNCFGYHVWTPIDGWSWLNAYKNRYGLVENNIHTQVRRPKASAYWFKKVATHNCLISLEVMEEFGGSASHLSD
>Mature_480_residues
MLAFPKEFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPDLFYDYVGPDTASDAYHQIESDLALLASLGHNSYRTSIQWT
RLIDDFEQATINPDGLAYYNRVINACLANGIRPVINLHHFDLPIALYQAYGGWESKHVVDIFVAFSKVCFEQFGDRVKDW
FVHNEPMVVVEGSYLMQFHYPAIVDGKKAVQVAYNLALATAKVIQAYRRGPAELSNGRIGTILNLTPAYPASQSEADMAA
AHFTELWNNDLFMEAAVHGKFPEELVAVLKKDGVLWQSTPEELALIAENRVDYLGLNFYHPKRVKAPDVIPVISPSWSPE
WYYDPYLMPGRRMNVDKGWEIYPEAVYDIAIKMRDHYDNIPWFLSENGVGISGEDRYRDETGQIQDDYRIQFLKEHLTYL
HKGIEAGSNCFGYHVWTPIDGWSWLNAYKNRYGLVENNIHTQVRRPKASAYWFKKVATHNCLISLEVMEEFGGSASHLSD

Specific function: Phospho-beta-D-glucosidase that seems to be involved in the degradation of glucomannan. Is also capable of hydrolyzing aryl-phospho-beta-D-glucosides, although very weakly, and plays only a minor role, if any, in the degradation of these substrates in viv

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=491, Percent_Identity=27.2912423625255, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI32481206, Length=490, Percent_Identity=27.7551020408163, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI13273313, Length=483, Percent_Identity=27.7432712215321, Blast_Score=162, Evalue=9e-40,
Organism=Homo sapiens, GI28376633, Length=476, Percent_Identity=27.3109243697479, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI24497614, Length=431, Percent_Identity=25.9860788863109, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI1789070, Length=488, Percent_Identity=30.327868852459, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI2367174, Length=492, Percent_Identity=29.0650406504065, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI2367270, Length=476, Percent_Identity=27.1008403361345, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17539390, Length=469, Percent_Identity=30.0639658848614, Blast_Score=197, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI17552856, Length=469, Percent_Identity=29.2110874200426, Blast_Score=195, Evalue=5e-50,
Organism=Drosophila melanogaster, GI21356577, Length=489, Percent_Identity=26.1758691206544, Blast_Score=161, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 55084; Mature: 55084

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAFPKEFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPDLFYDYVGPDTASDAYHQIES
CCCCCCHHCCCCCCCCCCCCCHHHHHHCCHHHHHCCCCCCEEEEECCCCCCHHHHHHHHH
DLALLASLGHNSYRTSIQWTRLIDDFEQATINPDGLAYYNRVINACLANGIRPVINLHHF
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHEEECC
DLPIALYQAYGGWESKHVVDIFVAFSKVCFEQFGDRVKDWFVHNEPMVVVEGSYLMQFHY
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCEEEEEEC
PAIVDGKKAVQVAYNLALATAKVIQAYRRGPAELSNGRIGTILNLTPAYPASQSEADMAA
CEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHH
AHFTELWNNDLFMEAAVHGKFPEELVAVLKKDGVLWQSTPEELALIAENRVDYLGLNFYH
HHHHHHHCCCEEEEEHHCCCCHHHHHHHHHHCCCEECCCHHHHHHHHCCCCCEEECCCCC
PKRVKAPDVIPVISPSWSPEWYYDPYLMPGRRMNVDKGWEIYPEAVYDIAIKMRDHYDNI
CCCCCCCCEEEEECCCCCCCCEECCEECCCCCCCCCCCCCCCHHHHHHHHEEEHHCCCCC
PWFLSENGVGISGEDRYRDETGQIQDDYRIQFLKEHLTYLHKGIEAGSNCFGYHVWTPID
CEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
GWSWLNAYKNRYGLVENNIHTQVRRPKASAYWFKKVATHNCLISLEVMEEFGGSASHLSD
CHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCEEEEEEHHHHHCCCCCCCCC
>Mature Secondary Structure
MLAFPKEFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPDLFYDYVGPDTASDAYHQIES
CCCCCCHHCCCCCCCCCCCCCHHHHHHCCHHHHHCCCCCCEEEEECCCCCCHHHHHHHHH
DLALLASLGHNSYRTSIQWTRLIDDFEQATINPDGLAYYNRVINACLANGIRPVINLHHF
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHEEECC
DLPIALYQAYGGWESKHVVDIFVAFSKVCFEQFGDRVKDWFVHNEPMVVVEGSYLMQFHY
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCEEEEEEC
PAIVDGKKAVQVAYNLALATAKVIQAYRRGPAELSNGRIGTILNLTPAYPASQSEADMAA
CEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHH
AHFTELWNNDLFMEAAVHGKFPEELVAVLKKDGVLWQSTPEELALIAENRVDYLGLNFYH
HHHHHHHCCCEEEEEHHCCCCHHHHHHHHHHCCCEECCCHHHHHHHHCCCCCEEECCCCC
PKRVKAPDVIPVISPSWSPEWYYDPYLMPGRRMNVDKGWEIYPEAVYDIAIKMRDHYDNI
CCCCCCCCEEEEECCCCCCCCEECCEECCCCCCCCCCCCCCCHHHHHHHHEEEHHCCCCC
PWFLSENGVGISGEDRYRDETGQIQDDYRIQFLKEHLTYLHKGIEAGSNCFGYHVWTPID
CEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
GWSWLNAYKNRYGLVENNIHTQVRRPKASAYWFKKVATHNCLISLEVMEEFGGSASHLSD
CHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCEEEEEEHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]