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The map label for this gene is murD [H]

Identifier: 19746474

GI number: 19746474

Start: 1272419

End: 1273777

Strand: Reverse

Name: murD [H]

Synonym: spyM18_1542

Alternate gene names: 19746474

Gene position: 1273777-1272419 (Counterclockwise)

Preceding gene: 19746475

Following gene: 161484699

Centisome position: 67.22

GC content: 40.4

Gene sequence:

>1359_bases
ATGAAAGTGATAAGTAATTTTCAAAACAAAAAAATATTAATATTGGGGTTAGCCAAATCGGGCGAAGCAGCAGCAAAATT
ATTGACCAAACTTGGTGCTTTAGTGACTGTTAATGATAGTAAACCATTTGACCAAAATCCAGCGGCACAAGCCTTGTTGG
AAGAGGGGATTAAGGTCATTTGTGGTAGCCACCCAGTAGAATTATTAGATGAGAACTTTGAGTACATGGTTAAAAACCCT
GGGATTCCTTATGATAATCCTATGGTTAAACGCGCCCTTGCAAAGGAAATTCCCATCTTGACTGAAGTAGAATTGGCTTA
TTTCGTATCTGAAGCGCCTATTATCGGGATTACAGGATCAAACGGGAAGACAACCACAACGACAATGATTGCCGATGTTT
TGAATGCTGGCGGGCAATCTGCACTCTTATCTGGAAACATTGGTTATCCTGCTTCAAAAGTTGTTCAAAAAGCAATTGCT
GGTGATACTTTGGTGATGGAATTGTCCTCTTTTCAATTAGTGGGAGTGAATGCTTTTCGCCCTCATATTGCTGTCATCAC
TAATTTAATGCCGACTCACCTGGACTATCATGGCAGTTTTGAGGATTATGTTGCTGCTAAATGGATGATTCAAGCTCAGA
TGACAGAATCAGACTACCTTATTTTAAATGCTAATCAAGAGATTTCAGCAACTCTAGCTAAGACCACCAAAGCAACAGTG
ATTCCTTTTTCAACTCAAAAAGTGGTTGATGGAGCTTATCTGAATGATGGAATACTCTATTTTAAAGAACAGGCGATTAT
AGCTGCAACTGACTTAGGTGTCCCAGGTAGCCACAACATTGAAAATGCCCTAGCAACTATTGCAGTTGCCAAGTTATCTG
GTATTGCTGATGATATTATTGCCCAGTGCCTTTCACATTTTGGAGGCGTTAAACATCGTTTGCAACGGGTTGGTCAAATC
AAAGATATTACCTTCTACAATGACAGTAAGTCAACCAATATTTTAGCCACTCAAAAAGCTTTATCAGGTTTTGATAACAG
TCGCTTGATTTTGATTGCTGGCGGTCTAGATCGTGGCAATGAATTTGACGATTTGGTGCCAGACCTTTTAGGACTTAAGC
AGATGATTATTTTGGGAGAATCCGCAGAGCGTATGAAGCGAGCTGCTAACAAAGCAGAGGTCTCTTATCTTGAAGCTAGA
AATGTGGCAGAAGCAACAGAGCTTGCTTTTAAGCTGGCCCAAACAGGCGATACTATCTTGCTTAGCCCAGCCAATGCTAG
CTGGGATATGTATCCTAATTTTGAGGTTCGTGGGGATGAATTTTTGGCAACCTTTGATTGTTTAAGAGGAGATGCCTAA

Upstream 100 bases:

>100_bases
ATTCTAATTCTAAACTCAAGAACGGTATTTTTAAAATGTTGAAAAAGCATCTAAATAAAGGTAAAATAGAAATCAAATAG
AATGGATAGTGTGAGCTTAG

Downstream 100 bases:

>100_bases
TGCCTAAGAAGATTTTATTTACAGGTGGTGGAACTGTAGGTCATGTCACCTTGAACCTCATTCTCATACCAAAATTTATC
AAGGACGGTTGGGAAGTACA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFDQNPAAQALLEEGIKVICGSHPVELLDENFEYMVKNP
GIPYDNPMVKRALAKEIPILTEVELAYFVSEAPIIGITGSNGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIA
GDTLVMELSSFQLVGVNAFRPHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESDYLILNANQEISATLAKTTKATV
IPFSTQKVVDGAYLNDGILYFKEQAIIAATDLGVPGSHNIENALATIAVAKLSGIADDIIAQCLSHFGGVKHRLQRVGQI
KDITFYNDSKSTNILATQKALSGFDNSRLILIAGGLDRGNEFDDLVPDLLGLKQMIILGESAERMKRAANKAEVSYLEAR
NVAEATELAFKLAQTGDTILLSPANASWDMYPNFEVRGDEFLATFDCLRGDA

Sequences:

>Translated_452_residues
MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFDQNPAAQALLEEGIKVICGSHPVELLDENFEYMVKNP
GIPYDNPMVKRALAKEIPILTEVELAYFVSEAPIIGITGSNGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIA
GDTLVMELSSFQLVGVNAFRPHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESDYLILNANQEISATLAKTTKATV
IPFSTQKVVDGAYLNDGILYFKEQAIIAATDLGVPGSHNIENALATIAVAKLSGIADDIIAQCLSHFGGVKHRLQRVGQI
KDITFYNDSKSTNILATQKALSGFDNSRLILIAGGLDRGNEFDDLVPDLLGLKQMIILGESAERMKRAANKAEVSYLEAR
NVAEATELAFKLAQTGDTILLSPANASWDMYPNFEVRGDEFLATFDCLRGDA
>Mature_452_residues
MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFDQNPAAQALLEEGIKVICGSHPVELLDENFEYMVKNP
GIPYDNPMVKRALAKEIPILTEVELAYFVSEAPIIGITGSNGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIA
GDTLVMELSSFQLVGVNAFRPHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESDYLILNANQEISATLAKTTKATV
IPFSTQKVVDGAYLNDGILYFKEQAIIAATDLGVPGSHNIENALATIAVAKLSGIADDIIAQCLSHFGGVKHRLQRVGQI
KDITFYNDSKSTNILATQKALSGFDNSRLILIAGGLDRGNEFDDLVPDLLGLKQMIILGESAERMKRAANKAEVSYLEAR
NVAEATELAFKLAQTGDTILLSPANASWDMYPNFEVRGDEFLATFDCLRGDA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=445, Percent_Identity=29.6629213483146, Blast_Score=152, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 48713; Mature: 48713

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFDQNPAAQALLEEGIKVI
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCCHHEE
CGSHPVELLDENFEYMVKNPGIPYDNPMVKRALAKEIPILTEVELAYFVSEAPIIGITGS
ECCCCHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCCCEEEHHHHHHCCCCCEEEEECC
NGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIAGDTLVMELSSFQLVGVNAFR
CCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEECCEEEEECCCCC
PHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESDYLILNANQEISATLAKTTKATV
CHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEEECCCCEEEEECCCCHHHHHHHCCCEEE
IPFSTQKVVDGAYLNDGILYFKEQAIIAATDLGVPGSHNIENALATIAVAKLSGIADDII
EECCHHHHHCCEEECCCEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHH
AQCLSHFGGVKHRLQRVGQIKDITFYNDSKSTNILATQKALSGFDNSRLILIAGGLDRGN
HHHHHHHCHHHHHHHHCCCEEEEEEECCCCCCEEEEEHHHHCCCCCCEEEEEECCCCCCC
EFDDLVPDLLGLKQMIILGESAERMKRAANKAEVSYLEARNVAEATELAFKLAQTGDTIL
CHHHHHHHHHHHHHHEEECCCHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEE
LSPANASWDMYPNFEVRGDEFLATFDCLRGDA
EECCCCCCCCCCCEEECCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFDQNPAAQALLEEGIKVI
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCCHHEE
CGSHPVELLDENFEYMVKNPGIPYDNPMVKRALAKEIPILTEVELAYFVSEAPIIGITGS
ECCCCHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCCCEEEHHHHHHCCCCCEEEEECC
NGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIAGDTLVMELSSFQLVGVNAFR
CCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEECCEEEEECCCCC
PHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESDYLILNANQEISATLAKTTKATV
CHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEEECCCCEEEEECCCCHHHHHHHCCCEEE
IPFSTQKVVDGAYLNDGILYFKEQAIIAATDLGVPGSHNIENALATIAVAKLSGIADDII
EECCHHHHHCCEEECCCEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHH
AQCLSHFGGVKHRLQRVGQIKDITFYNDSKSTNILATQKALSGFDNSRLILIAGGLDRGN
HHHHHHHCHHHHHHHHCCCEEEEEEECCCCCCEEEEEHHHHCCCCCCEEEEEECCCCCCC
EFDDLVPDLLGLKQMIILGESAERMKRAANKAEVSYLEARNVAEATELAFKLAQTGDTIL
CHHHHHHHHHHHHHHEEECCCHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEE
LSPANASWDMYPNFEVRGDEFLATFDCLRGDA
EECCCCCCCCCCCEEECCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]