Click here to switch to the map view.

The map label for this gene is phr [H]

Identifier: 19746457

GI number: 19746457

Start: 1253917

End: 1255326

Strand: Reverse

Name: phr [H]

Synonym: spyM18_1523

Alternate gene names: 19746457

Gene position: 1255326-1253917 (Counterclockwise)

Preceding gene: 19746458

Following gene: 19746456

Centisome position: 66.24

GC content: 37.38

Gene sequence:

>1410_bases
ATGGTTTCAGTGATGTGGTTTCGTAGAGATTTAAGGATTGATGATCAAAAAGCTTTAGCTCATGTTATTGCCAGTCAATC
ACCAACCTTATGTGTGTTTCAATTTAACAAGGAGCAATTAAATCTGCTACATAGTCGGAATCAATCTAGTTTTATTACTA
GCGTTTTGGCTTTTAGAAAGTTATTAAAATGTGAAGGTATTGACTTATATCTAATGTATGGAGACTTAATGACCTGTTTT
GAGCAATTATTGACACAATTAAAAGACTGGACAGATGTGTATTTTAACTATGATGAATCTGGATATGGTCGTTTACGTGA
TCAAAAAGCAGCACAATTTTTCAAAAAAAATGGGATTGCCGTTCATACCTACCAAGATCACTATTTACATGGTAGTCAAG
AGATCATTAACCAATCAGGTCAGCCTTATAAAGTTTTTACGCCCTACTATAGGATTTGGCAAAATTATCCGAAAGAGACT
CCTATTAAAGTAGAACTATCGCAAGGCAGGTGGTTAAATCTTGAGACACCAGATGATGTTTTAAGGACTGTTGAGAGCTT
TAAAGATGAAAAATATCAGGATGTTGCAACTTTTGATGAAGCGTCAAAACAACTAAATCGATTTATTCAAGACCAATTAG
CAGCTTATCACGCTAATCGAGATTTTCCAGCGCAGCTAGGGACGAGTCGGTTGTCTCCTTTTTTAAGAATAGGAGCTATT
GGCATTCGCACGGTATATCACGCTGTGCGTCAAGCACCAAATAGTCTAGGGCAAGCTACTTTTTTAAAAGAATTAGCTTG
GAGAGACTTTTACAATATGGTCTATGTGGCTTATCCTGATCAGAAAACTCAGCCTATCCAAAAAGCCTTTTCTCAAATCG
AATGGGTTAATAATCCTGACTGGTTTCAGTTGTGGAAAGAAGGTAAAACGGGTTACCCTATTGTCGATGCTGCAATGCTT
CAGTTACAAAAGACAGGATGGATGCATAATCGTTTACGAATGATAGTAGCTTCTTTTTTAACTAAAGATCTTTTATGTGA
TTGGCGTTTAGGAGAGCAGTATTTTCAACAACAACTAATTGATTATGATGCTGCCAGTAATATTGGAGGATGGCAGTGGG
CTGCTTCCACTGGAACAGATGCTGTTCCTTATTTTAGAATATTTAATCCTGTTACTCAAGGTAAGCGCTTTGATCCCAAA
GGCGAGTTTATTAAAGCGTATTTGCCTCAACTAGAACACGTTCCAGAGAAGTATCTCCACGAACCGTGGAAGATGCCAAA
AAACCTTCAAGAGAGTGTGTCCTGTATTATTGGCACAGACTATCCTCAACCAATTGTCGATCATGCTAAACAACGTGAAC
AAGCTATTGCAAAGTATGAGTGGGCAAAAGAAAAAGCAAAAATAGAATGA

Upstream 100 bases:

>100_bases
CTTTTATAATTTGCTAAGGCATTTTATAGGAGATTACTTTTCTTTTTGTTAAAATAAAAAGAAATAGTTGCATTATTTGC
ATAGAAGAAAGGGATCTGTT

Downstream 100 bases:

>100_bases
CTGTTCTCATTATAAGAGTTTAAACAAAAAGTTATCATGAATTAAGATTATTTAATTTAAAAAATAAGTTGACTTTATTT
AGATTATTTGATACTATTCA

Product: deoxyribodipyrimidine photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MVSVMWFRRDLRIDDQKALAHVIASQSPTLCVFQFNKEQLNLLHSRNQSSFITSVLAFRKLLKCEGIDLYLMYGDLMTCF
EQLLTQLKDWTDVYFNYDESGYGRLRDQKAAQFFKKNGIAVHTYQDHYLHGSQEIINQSGQPYKVFTPYYRIWQNYPKET
PIKVELSQGRWLNLETPDDVLRTVESFKDEKYQDVATFDEASKQLNRFIQDQLAAYHANRDFPAQLGTSRLSPFLRIGAI
GIRTVYHAVRQAPNSLGQATFLKELAWRDFYNMVYVAYPDQKTQPIQKAFSQIEWVNNPDWFQLWKEGKTGYPIVDAAML
QLQKTGWMHNRLRMIVASFLTKDLLCDWRLGEQYFQQQLIDYDAASNIGGWQWAASTGTDAVPYFRIFNPVTQGKRFDPK
GEFIKAYLPQLEHVPEKYLHEPWKMPKNLQESVSCIIGTDYPQPIVDHAKQREQAIAKYEWAKEKAKIE

Sequences:

>Translated_469_residues
MVSVMWFRRDLRIDDQKALAHVIASQSPTLCVFQFNKEQLNLLHSRNQSSFITSVLAFRKLLKCEGIDLYLMYGDLMTCF
EQLLTQLKDWTDVYFNYDESGYGRLRDQKAAQFFKKNGIAVHTYQDHYLHGSQEIINQSGQPYKVFTPYYRIWQNYPKET
PIKVELSQGRWLNLETPDDVLRTVESFKDEKYQDVATFDEASKQLNRFIQDQLAAYHANRDFPAQLGTSRLSPFLRIGAI
GIRTVYHAVRQAPNSLGQATFLKELAWRDFYNMVYVAYPDQKTQPIQKAFSQIEWVNNPDWFQLWKEGKTGYPIVDAAML
QLQKTGWMHNRLRMIVASFLTKDLLCDWRLGEQYFQQQLIDYDAASNIGGWQWAASTGTDAVPYFRIFNPVTQGKRFDPK
GEFIKAYLPQLEHVPEKYLHEPWKMPKNLQESVSCIIGTDYPQPIVDHAKQREQAIAKYEWAKEKAKIE
>Mature_469_residues
MVSVMWFRRDLRIDDQKALAHVIASQSPTLCVFQFNKEQLNLLHSRNQSSFITSVLAFRKLLKCEGIDLYLMYGDLMTCF
EQLLTQLKDWTDVYFNYDESGYGRLRDQKAAQFFKKNGIAVHTYQDHYLHGSQEIINQSGQPYKVFTPYYRIWQNYPKET
PIKVELSQGRWLNLETPDDVLRTVESFKDEKYQDVATFDEASKQLNRFIQDQLAAYHANRDFPAQLGTSRLSPFLRIGAI
GIRTVYHAVRQAPNSLGQATFLKELAWRDFYNMVYVAYPDQKTQPIQKAFSQIEWVNNPDWFQLWKEGKTGYPIVDAAML
QLQKTGWMHNRLRMIVASFLTKDLLCDWRLGEQYFQQQLIDYDAASNIGGWQWAASTGTDAVPYFRIFNPVTQGKRFDPK
GEFIKAYLPQLEHVPEKYLHEPWKMPKNLQESVSCIIGTDYPQPIVDHAKQREQAIAKYEWAKEKAKIE

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=495, Percent_Identity=28.8888888888889, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI4758072, Length=502, Percent_Identity=29.4820717131474, Blast_Score=195, Evalue=8e-50,
Organism=Homo sapiens, GI188536103, Length=419, Percent_Identity=29.5942720763723, Blast_Score=189, Evalue=5e-48,
Organism=Escherichia coli, GI1786926, Length=477, Percent_Identity=38.7840670859539, Blast_Score=301, Evalue=6e-83,
Organism=Saccharomyces cerevisiae, GI6324962, Length=363, Percent_Identity=35.2617079889807, Blast_Score=197, Evalue=2e-51,
Organism=Drosophila melanogaster, GI17137248, Length=491, Percent_Identity=27.9022403258656, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24585455, Length=491, Percent_Identity=27.9022403258656, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24648152, Length=232, Percent_Identity=33.6206896551724, Blast_Score=145, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 54921; Mature: 54921

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSVMWFRRDLRIDDQKALAHVIASQSPTLCVFQFNKEQLNLLHSRNQSSFITSVLAFRK
CCEEEEEHHCCCCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHCCCHHHHHHHHHHHHH
LLKCEGIDLYLMYGDLMTCFEQLLTQLKDWTDVYFNYDESGYGRLRDQKAAQFFKKNGIA
HHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHCCCE
VHTYQDHYLHGSQEIINQSGQPYKVFTPYYRIWQNYPKETPIKVELSQGRWLNLETPDDV
EEEEHHHHCCCCHHHHCCCCCCEEEECHHHHHHHCCCCCCCEEEEECCCCEECCCCHHHH
LRTVESFKDEKYQDVATFDEASKQLNRFIQDQLAAYHANRDFPAQLGTSRLSPFLRIGAI
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCHHHHCHHHHHHHH
GIRTVYHAVRQAPNSLGQATFLKELAWRDFYNMVYVAYPDQKTQPIQKAFSQIEWVNNPD
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHCCCCCCH
WFQLWKEGKTGYPIVDAAMLQLQKTGWMHNRLRMIVASFLTKDLLCDWRLGEQYFQQQLI
HHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
DYDAASNIGGWQWAASTGTDAVPYFRIFNPVTQGKRFDPKGEFIKAYLPQLEHVPEKYLH
HHHCCCCCCCCEEECCCCCCCCCHHHHHCHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHC
EPWKMPKNLQESVSCIIGTDYPQPIVDHAKQREQAIAKYEWAKEKAKIE
CHHCCCCCHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVSVMWFRRDLRIDDQKALAHVIASQSPTLCVFQFNKEQLNLLHSRNQSSFITSVLAFRK
CCEEEEEHHCCCCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHCCCHHHHHHHHHHHHH
LLKCEGIDLYLMYGDLMTCFEQLLTQLKDWTDVYFNYDESGYGRLRDQKAAQFFKKNGIA
HHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHCCCE
VHTYQDHYLHGSQEIINQSGQPYKVFTPYYRIWQNYPKETPIKVELSQGRWLNLETPDDV
EEEEHHHHCCCCHHHHCCCCCCEEEECHHHHHHHCCCCCCCEEEEECCCCEECCCCHHHH
LRTVESFKDEKYQDVATFDEASKQLNRFIQDQLAAYHANRDFPAQLGTSRLSPFLRIGAI
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCHHHHCHHHHHHHH
GIRTVYHAVRQAPNSLGQATFLKELAWRDFYNMVYVAYPDQKTQPIQKAFSQIEWVNNPD
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHCCCCCCH
WFQLWKEGKTGYPIVDAAMLQLQKTGWMHNRLRMIVASFLTKDLLCDWRLGEQYFQQQLI
HHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
DYDAASNIGGWQWAASTGTDAVPYFRIFNPVTQGKRFDPKGEFIKAYLPQLEHVPEKYLH
HHHCCCCCCCCEEECCCCCCCCCHHHHHCHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHC
EPWKMPKNLQESVSCIIGTDYPQPIVDHAKQREQAIAKYEWAKEKAKIE
CHHCCCCCHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678007 [H]