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The map label for this gene is recN [H]

Identifier: 19746449

GI number: 19746449

Start: 1245465

End: 1247126

Strand: Reverse

Name: recN [H]

Synonym: spyM18_1513

Alternate gene names: 19746449

Gene position: 1247126-1245465 (Counterclockwise)

Preceding gene: 19746450

Following gene: 19746448

Centisome position: 65.81

GC content: 39.95

Gene sequence:

>1662_bases
ATGTTATTAGAAATTTCTATTAAAAATTTTGCTATTATTGATGAAATTTCCCTAAATTTTGAAAATGGTATGACGGTTTT
GACGGGTGAAACTGGAGCAGGAAAGTCTATTATCATTGATGCTATGAATATGATGCTTGGTGCGCGTGCTAGTACAGAAG
TGATTCGTCGCGGAGCTAATAAAGCAGAAATTGAGGGGTTCTTTTCGGTAGATGCTACCCCAGAACTGGTTGCGTGTCTG
GAATCATCAGGTATTGCTATGGAAGAAGAACTGATTATTCGCCGGGATATCTTTGCCAATGGCAGAAGCGTGAGCCGCAT
TAATGGTCAGATGGTTAACCTAGCCACCTTAAAACAGGTCGGACAGTTTTTGGTAGATATTCATGGGCAACATGACCAAG
AAGAATTAATGCGACCACAGCTCCACCAGCAAATTTTGGACGCTTTTGGTGATAAGGCTTTTGAGCAATTGAAAGAGAAC
TATCAGCTTATTTTTGATCGTTATAAAAGCTTGCGTCGCCAGGTTATTGACAAACAAAAAAATGAAAAGGAACACAAAGA
TCGTATTGATATGTTGGCCTTTCAGATAGCAGAAATTGAAGCTGCTGCCTTGAGTCGAGGTGAGGACGACCGGTTAAATC
AAGAACGTGATCGCTTAATGAACCACAAACAAATTGCTGATACCCTGACCAATGCCTACGTCATGCTAGATAATGACGAT
TTTTCAAGCCTATCCAATATTCGCTCTAGCATGAATGACTTACTATCAATTGAGCAGTTTGATTCAGAGTACAAAGGGAT
GTCGACTTCGATTTCTGAAGCCTATTATATCCTTGAAGAAGTGAGCAAGCAATTATCAGATACCATTGACCAACTGGATT
TTGATGGTGGGCGATTACAGGAAATTGAATTTCGCTTGGATACACTCAATAGCTTAACCCGTAAATACGGTGGTAATATG
AATGATGTGCTTGACTACTACGATAATATCGTCAAAGAATACCAGCTATTAACAGGAGATGACTTGTCTTCAGGGGATCT
AGAGGCAGAATTGAAAGCCTTAGAAAAACAATTGGTTGCTGCAGCCACTAACTTGAGTGAACGCCGCCATCAATTGGCGG
AGCAATTAGAAGCTGAGATTAAAGCTGAACTTAAGGAACTTTGTATGGAAAAAGCAGATTTCAAAGTTCACTTTACCACA
TCAAAATTCAATCGTGATGGTAATGAAAGCTTGGAATTTTACATCTCTACTAATCCAGGCGAAGGATTCAAGCCCCTTGT
CAAGGTGGCTTCTGGAGGAGAGTTATCACGCCTTATGTTAGCCATCAAGGCTGCTATTTCTAGAAAAGAAGATAAGACGA
GTATTGTTTTTGATGAAGTAGATACAGGTGTTTCTGGTCGCGTTGCGCAAGCCATTGCCCAAAAAATTTATAAAATTGGG
CGACATGGGCAAGTTCTCGCTATTTCACACTTACCTCAAGTTATTGCTATTGCTGATTATCAGTATTTTATTTCTAAAGA
AAGTAAAGAAGAGTCCACCGTTTCTAAGGTAAGGTTATTAACTCCTGAAGAACGTGTGGAAGAAATTGCTAGCATGATTG
CAGGAACAGATATGACACAAGCTGCTCTTACGCAGGCTCGTGAACTACTTGCCAAACATTAA

Upstream 100 bases:

>100_bases
ACTGTTAATTGCAAAGTCTCCTTCAGAAGCAGATATGATACGCCAAGAAATTTTGCTTTGGATGCAGGGTATAACCTAGA
AAAAAATAGGGGAGAAAGTG

Downstream 100 bases:

>100_bases
GATTATGTTTGGTATTTGATATTTTCATAATTAGATGAAATTGATTTTTTAGGTGCTAAAATAATCATTGTGCTAGACCA
TTTTTCCTCTGTTAATCGGG

Product: DNA repair and genetic recombination protein

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNMMLGARASTEVIRRGANKAEIEGFFSVDATPELVACL
ESSGIAMEEELIIRRDIFANGRSVSRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKEN
YQLIFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDRLMNHKQIADTLTNAYVMLDNDD
FSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYYILEEVSKQLSDTIDQLDFDGGRLQEIEFRLDTLNSLTRKYGGNM
NDVLDYYDNIVKEYQLLTGDDLSSGDLEAELKALEKQLVAAATNLSERRHQLAEQLEAEIKAELKELCMEKADFKVHFTT
SKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISRKEDKTSIVFDEVDTGVSGRVAQAIAQKIYKIG
RHGQVLAISHLPQVIAIADYQYFISKESKEESTVSKVRLLTPEERVEEIASMIAGTDMTQAALTQARELLAKH

Sequences:

>Translated_553_residues
MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNMMLGARASTEVIRRGANKAEIEGFFSVDATPELVACL
ESSGIAMEEELIIRRDIFANGRSVSRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKEN
YQLIFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDRLMNHKQIADTLTNAYVMLDNDD
FSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYYILEEVSKQLSDTIDQLDFDGGRLQEIEFRLDTLNSLTRKYGGNM
NDVLDYYDNIVKEYQLLTGDDLSSGDLEAELKALEKQLVAAATNLSERRHQLAEQLEAEIKAELKELCMEKADFKVHFTT
SKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISRKEDKTSIVFDEVDTGVSGRVAQAIAQKIYKIG
RHGQVLAISHLPQVIAIADYQYFISKESKEESTVSKVRLLTPEERVEEIASMIAGTDMTQAALTQARELLAKH
>Mature_553_residues
MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNMMLGARASTEVIRRGANKAEIEGFFSVDATPELVACL
ESSGIAMEEELIIRRDIFANGRSVSRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKEN
YQLIFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDRLMNHKQIADTLTNAYVMLDNDD
FSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYYILEEVSKQLSDTIDQLDFDGGRLQEIEFRLDTLNSLTRKYGGNM
NDVLDYYDNIVKEYQLLTGDDLSSGDLEAELKALEKQLVAAATNLSERRHQLAEQLEAEIKAELKELCMEKADFKVHFTT
SKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISRKEDKTSIVFDEVDTGVSGRVAQAIAQKIYKIG
RHGQVLAISHLPQVIAIADYQYFISKESKEESTVSKVRLLTPEERVEEIASMIAGTDMTQAALTQARELLAKH

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=563, Percent_Identity=34.4582593250444, Blast_Score=330, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 62228; Mature: 62228

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNMMLGARASTEVIRRGAN
CEEEEEECCEEEEEHEEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCC
KAEIEGFFSVDATPELVACLESSGIAMEEELIIRRDIFANGRSVSRINGQMVNLATLKQV
HHHCCEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHCCCCEEHHHHHHHH
GQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKENYQLIFDRYKSLRRQVIDKQK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDRLMNHKQIADTLTNAYVMLDNDD
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCC
FSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYYILEEVSKQLSDTIDQLDFDGGRLQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
EIEFRLDTLNSLTRKYGGNMNDVLDYYDNIVKEYQLLTGDDLSSGDLEAELKALEKQLVA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
AATNLSERRHQLAEQLEAEIKAELKELCMEKADFKVHFTTSKFNRDGNESLEFYISTNPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCCCCCEEEEEECCCC
EGFKPLVKVASGGELSRLMLAIKAAISRKEDKTSIVFDEVDTGVSGRVAQAIAQKIYKIG
CCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHC
RHGQVLAISHLPQVIAIADYQYFISKESKEESTVSKVRLLTPEERVEEIASMIAGTDMTQ
CCCCEEEHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHH
AALTQARELLAKH
HHHHHHHHHHHCC
>Mature Secondary Structure
MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNMMLGARASTEVIRRGAN
CEEEEEECCEEEEEHEEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCC
KAEIEGFFSVDATPELVACLESSGIAMEEELIIRRDIFANGRSVSRINGQMVNLATLKQV
HHHCCEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHCCCCEEHHHHHHHH
GQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKENYQLIFDRYKSLRRQVIDKQK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDRLMNHKQIADTLTNAYVMLDNDD
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCC
FSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYYILEEVSKQLSDTIDQLDFDGGRLQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
EIEFRLDTLNSLTRKYGGNMNDVLDYYDNIVKEYQLLTGDDLSSGDLEAELKALEKQLVA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
AATNLSERRHQLAEQLEAEIKAELKELCMEKADFKVHFTTSKFNRDGNESLEFYISTNPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCCCCCEEEEEECCCC
EGFKPLVKVASGGELSRLMLAIKAAISRKEDKTSIVFDEVDTGVSGRVAQAIAQKIYKIG
CCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHC
RHGQVLAISHLPQVIAIADYQYFISKESKEESTVSKVRLLTPEERVEEIASMIAGTDMTQ
CCCCEEEHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHH
AALTQARELLAKH
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]