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The map label for this gene is alaS [H]

Identifier: 19746355

GI number: 19746355

Start: 1167753

End: 1170371

Strand: Reverse

Name: alaS [H]

Synonym: spyM18_1399

Alternate gene names: 19746355

Gene position: 1170371-1167753 (Counterclockwise)

Preceding gene: 19746356

Following gene: 19746354

Centisome position: 61.76

GC content: 42.27

Gene sequence:

>2619_bases
ATGAAAGAATTATCGTCTGCACAAATCCGCCAAATGTGGTTGGATTTCTGGAAATCTAAAGGACATTGCGTTGAGCCTTC
AGCTAACTTGGTTCCTGTGAATGACCCAACGCTTCTTTGGATCAACTCAGGTGTTGCAACCTTGAAAAAATACTTTGATG
GTTCAGTGATTCCAGAAAATCCACGTATTACCAATGCACAAAAATCAATTCGTACTAATGATATTGAAAATGTTGGTAAA
ACAGCACGTCACCATACTATGTTTGAAATGCTTGGTAACTTCTCAATTGGAGACTATTTCCGTAATGAAGCTATTGGGTG
GGGATTTGAACTCTTGACAAGTCCTGAATGGTTTGACTTTCCTAAGGACAAGCTCTACATGACTTATTACCCAGATGACA
AGGATTCGTATAACCGTTGGATTGCTTGTGGCGTTGAACCAAGTCACTTGGTGCCAATCGAGGATAACTTCTGGGAAATC
GGTGCTGGTCCTTCAGGTCCAGATACGGAGATTTTCTTCGACCGTGGTGAAGATTTCGATCCAGAAAATATCGGAATTCG
CCTCTTGGCTGAAGATATCGAAAACGATCGTTACATCGAAATCTGGAACATCGTTCTCTCACAATTCAATGCTGATCCTG
CTGTGCCACGTTCAGAATACAAAGAATTACCAAACAAAAACATTGATACGGGTGCTGGTCTTGAACGTCTTGCAGCTGTT
ATGCAAGGGGCAAAAACAAACTTTGAAACTGACCTTTTCATGCCTATCATCCGTGAAGTAGAAAAATTGTCAGGTAAAAC
TTACGATCCAGATGGCGACAACATGAGTTTCAAGGTTATCGCTGACCACATCCGTGCGCTTTTATTTGCTATCGGTGATG
GTGCGCTTCCTGGAAATGAAGGTCGTGGTTACGTTCTTCGTCGTCTTCTCCGTCGTGCGGTTATGCATGGCCGCCGTCTT
GGCATCAACGAAACTTTCCTTTACAAATTGGTTCTAACTGTTGGACAAATCATGGAAAGCTACTACCCAGAAGTGCTTGA
AAAACGTGATTTTATCGAGAAAATCGTTAAACGTGAGGAAGAAACATTTGCTCGTACTATCGATGCAGGTAGCGGTCACT
TGGATTCATTGCTTGCGCAGCTTAAAGCTGAAGGTAAAGATACACTTGAAGGTAAAGATATCTTCAAACTTTATGATACT
TATGGATTCCCGGTTGAATTGACAGAGGAATTGGCAGAAGATGCAGGATACAAGATTGACCACGAAGGCTTTAAGTCAGC
CATGAAAGAACAACAAGACCGTGCGCGTGCAGCTGTTGTTAAGGGTGGTTCAATGGGGATGCAAAATGAAACCCTAGCTG
GTATTGTTGAAGAATCACGATTCGAATACGACACATATAGTCTTGAATCAAGTCTTTCAGTCATCATCGCTGATAATGAA
CGTACCGAAGCTGTTTCAGAAGGTCAAGCCCTTCTTGTCTTTGCTCAAACACCATTCTATGCTGAAATGGGTGGACAGGT
TGCTGACACAGGTAGAATCAAAAATGATAAGGGTGACACAGTTGCTGAGGTTGTTGATGTTCAAAAAGCACCAAATGGTC
AACCTCTACACACTGTAAACGTTTTAGCATCACTTTCAGTTGGAACAAACTACACACTTGAAATCAACAAAGAGCGTCGT
TTGGCTGTTGAGAAAAACCACACAGCAACTCACTTGCTCCATGCAGCCCTTCACAATGTTATCGGTGAACACGCAACTCA
GGCTGGTTCATTGAACGAAGAAGAATTCTTGCGCTTTGATTTTACTCACTTTGAAGCAGTAAGCAATGAGGAACTTCGTC
ACATTGAACAAGAAGTTAATGAGCAAATTTGGAACGCTCTTACAATCACAACGACTGAAACTGACGTTGAAACCGCAAAA
GAGAGGGGAGCAATGGCGCTTTTTGGTGAGAAATATGGTAAAGTGGTTCGTGTGGTTCAAATTGGTAATTATTCTGTTGA
ACTTTGTGGTGGAACTCACTTAAATAATTCTTCAGAAATCGGTCTCTTCAAGATTGTCAAAGAAGAAGGTATTGGTTCAG
GCACTCGTCGTATTATTGCAGTTACTGGTAGACAAGCTTTTGAAGCTTATCGTAACCAAGAGGATGCCCTAAAAGAGATC
GCTGCTACTGTAAAAGCTCCGCAATTGAAAGATGCAGCAGCTAAAGTACAAGCTCTTAGCGACTCACTTCGTGATTTTCA
AAAAGAAAATGCAGAGCTTAAAGAAAAAGCAGCAGCTGCAGCAGCTGGTGATGTCTTTAAAGATGTTCAAGAAGCTAAGG
GCGTGCGCTTCATTGCTAGTCAAGTTGATGTTGCAGATGCAGGGGCACTTCGTACATTTGCTGATAACTGGAAACAAAAA
GACTACTCTGATGTGCTTGTTCTCGTAGCAGCTATCGGTGAGAAGGTTAATGTCCTTGTTGCAAGTAAAACTAAAGATGT
CCACGCTGGTAACATGATTAAAGAATTGGCACCAATTGTAGCAGGTCGTGGTGGAGGTAAACCAGACATGGCTATGGCAG
GTGGTAGCGATGCAAGTAAAATTGCAGAGCTGCTAGCAGCAGTTGCTGAAACAGTGTAA

Upstream 100 bases:

>100_bases
CTGATCTCCTAGCAAGAAAGACGAGCTGTGTCAACTGGTGTGATTACATTACTAGCTTCAGCATAACACTGTTTTTCTTA
CTCTTTGTATCTAAATTTTT

Downstream 100 bases:

>100_bases
ATCCATAAAAACCAAGTTATTTAGAACTTGGTTTTTTATATTTGAAATGACTATCATCATAACCTTTTTGACTATTATCA
TTGTCAAAAAGAAAAAGATA

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 872; Mature: 872

Protein sequence:

>872_residues
MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSGVATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGK
TARHHTMFEMLGNFSIGDYFRNEAIGWGFELLTSPEWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
GAGPSGPDTEIFFDRGEDFDPENIGIRLLAEDIENDRYIEIWNIVLSQFNADPAVPRSEYKELPNKNIDTGAGLERLAAV
MQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMSFKVIADHIRALLFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRL
GINETFLYKLVLTVGQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGKDIFKLYDT
YGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNETLAGIVEESRFEYDTYSLESSLSVIIADNE
RTEAVSEGQALLVFAQTPFYAEMGGQVADTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERR
LAVEKNHTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIWNALTITTTETDVETAK
ERGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGLFKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEI
AATVKAPQLKDAAAKVQALSDSLRDFQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIASQVDVADAGALRTFADNWKQK
DYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGKPDMAMAGGSDASKIAELLAAVAETV

Sequences:

>Translated_872_residues
MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSGVATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGK
TARHHTMFEMLGNFSIGDYFRNEAIGWGFELLTSPEWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
GAGPSGPDTEIFFDRGEDFDPENIGIRLLAEDIENDRYIEIWNIVLSQFNADPAVPRSEYKELPNKNIDTGAGLERLAAV
MQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMSFKVIADHIRALLFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRL
GINETFLYKLVLTVGQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGKDIFKLYDT
YGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNETLAGIVEESRFEYDTYSLESSLSVIIADNE
RTEAVSEGQALLVFAQTPFYAEMGGQVADTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERR
LAVEKNHTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIWNALTITTTETDVETAK
ERGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGLFKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEI
AATVKAPQLKDAAAKVQALSDSLRDFQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIASQVDVADAGALRTFADNWKQK
DYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGKPDMAMAGGSDASKIAELLAAVAETV
>Mature_872_residues
MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSGVATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGK
TARHHTMFEMLGNFSIGDYFRNEAIGWGFELLTSPEWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
GAGPSGPDTEIFFDRGEDFDPENIGIRLLAEDIENDRYIEIWNIVLSQFNADPAVPRSEYKELPNKNIDTGAGLERLAAV
MQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMSFKVIADHIRALLFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRL
GINETFLYKLVLTVGQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGKDIFKLYDT
YGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNETLAGIVEESRFEYDTYSLESSLSVIIADNE
RTEAVSEGQALLVFAQTPFYAEMGGQVADTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERR
LAVEKNHTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIWNALTITTTETDVETAK
ERGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGLFKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEI
AATVKAPQLKDAAAKVQALSDSLRDFQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIASQVDVADAGALRTFADNWKQK
DYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGKPDMAMAGGSDASKIAELLAAVAETV

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=976, Percent_Identity=34.0163934426229, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI38569417, Length=779, Percent_Identity=30.808729139923, Blast_Score=327, Evalue=2e-89,
Organism=Escherichia coli, GI1789048, Length=884, Percent_Identity=43.4389140271493, Blast_Score=671, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=971, Percent_Identity=30.8959835221421, Blast_Score=403, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17536681, Length=761, Percent_Identity=32.8515111695138, Blast_Score=340, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6324911, Length=811, Percent_Identity=37.6078914919852, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24582809, Length=972, Percent_Identity=32.5102880658436, Blast_Score=451, Evalue=1e-126,
Organism=Drosophila melanogaster, GI45552267, Length=972, Percent_Identity=32.5102880658436, Blast_Score=451, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24658214, Length=814, Percent_Identity=28.2555282555283, Blast_Score=273, Evalue=4e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR016040
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 96562; Mature: 96562

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSGVATLKKYFDGSVIPEN
CCCCHHHHHHHHHHHHHCCCCCEECCCCCEEECCCCEEEEECCCHHHHHHHHCCCCCCCC
PRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRNEAIGWGFELLTSPEWFDF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHCCCCCCCC
PKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEIGAGPSGPDTEIFFDRGEDFD
CCCCEEEEECCCCCCCCCCEEEECCCCCEEEECCCCCEEECCCCCCCCCCEEEECCCCCC
PENIGIRLLAEDIENDRYIEIWNIVLSQFNADPAVPRSEYKELPNKNIDTGAGLERLAAV
CCCCCEEEEEECCCCCCEEHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHH
MQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMSFKVIADHIRALLFAIGDGALPGNE
HHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
GRGYVLRRLLRRAVMHGRRLGINETFLYKLVLTVGQIMESYYPEVLEKRDFIEKIVKREE
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETFARTIDAGSGHLDSLLAQLKAEGKDTLEGKDIFKLYDTYGFPVELTEELAEDAGYKID
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCEEC
HEGFKSAMKEQQDRARAAVVKGGSMGMQNETLAGIVEESRFEYDTYSLESSLSVIIADNE
HHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHCCCCCCEECCCCCEEEEECCC
RTEAVSEGQALLVFAQTPFYAEMGGQVADTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVN
HHHHHCCCCEEEEEECCCCHHHHCCCEECCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHH
VLASLSVGTNYTLEINKERRLAVEKNHTATHLLHAALHNVIGEHATQAGSLNEEEFLRFD
HHHHHCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEC
FTHFEAVSNEELRHIEQEVNEQIWNALTITTTETDVETAKERGAMALFGEKYGKVVRVVQ
HHHHHHCCHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHCCCEEEHHHHHCCEEEEEE
IGNYSVELCGGTHLNNSSEIGLFKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEI
ECCEEEEEECCCCCCCCCCCCCEEEEHHCCCCCCCCEEEEECCHHHHHHHCCHHHHHHHH
AATVKAPQLKDAAAKVQALSDSLRDFQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIAS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEHHH
QVDVADAGALRTFADNWKQKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIV
HCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHH
AGRGGGKPDMAMAGGSDASKIAELLAAVAETV
HCCCCCCCCCEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSGVATLKKYFDGSVIPEN
CCCCHHHHHHHHHHHHHCCCCCEECCCCCEEECCCCEEEEECCCHHHHHHHHCCCCCCCC
PRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRNEAIGWGFELLTSPEWFDF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHCCCCCCCC
PKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEIGAGPSGPDTEIFFDRGEDFD
CCCCEEEEECCCCCCCCCCEEEECCCCCEEEECCCCCEEECCCCCCCCCCEEEECCCCCC
PENIGIRLLAEDIENDRYIEIWNIVLSQFNADPAVPRSEYKELPNKNIDTGAGLERLAAV
CCCCCEEEEEECCCCCCEEHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHH
MQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMSFKVIADHIRALLFAIGDGALPGNE
HHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
GRGYVLRRLLRRAVMHGRRLGINETFLYKLVLTVGQIMESYYPEVLEKRDFIEKIVKREE
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETFARTIDAGSGHLDSLLAQLKAEGKDTLEGKDIFKLYDTYGFPVELTEELAEDAGYKID
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCEEC
HEGFKSAMKEQQDRARAAVVKGGSMGMQNETLAGIVEESRFEYDTYSLESSLSVIIADNE
HHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHCCCCCCEECCCCCEEEEECCC
RTEAVSEGQALLVFAQTPFYAEMGGQVADTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVN
HHHHHCCCCEEEEEECCCCHHHHCCCEECCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHH
VLASLSVGTNYTLEINKERRLAVEKNHTATHLLHAALHNVIGEHATQAGSLNEEEFLRFD
HHHHHCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEC
FTHFEAVSNEELRHIEQEVNEQIWNALTITTTETDVETAKERGAMALFGEKYGKVVRVVQ
HHHHHHCCHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHCCCEEEHHHHHCCEEEEEE
IGNYSVELCGGTHLNNSSEIGLFKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEI
ECCEEEEEECCCCCCCCCCCCCEEEEHHCCCCCCCCEEEEECCHHHHHHHCCHHHHHHHH
AATVKAPQLKDAAAKVQALSDSLRDFQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIAS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEHHH
QVDVADAGALRTFADNWKQKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIV
HCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHH
AGRGGGKPDMAMAGGSDASKIAELLAAVAETV
HCCCCCCCCCEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA