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The map label for this gene is ptcB [H]

Identifier: 19746302

GI number: 19746302

Start: 1112800

End: 1113111

Strand: Reverse

Name: ptcB [H]

Synonym: spyM18_1334

Alternate gene names: 19746302

Gene position: 1113111-1112800 (Counterclockwise)

Preceding gene: 19746303

Following gene: 19746301

Centisome position: 58.74

GC content: 39.1

Gene sequence:

>312_bases
ATGAAATCTATTATGTTAGTTTGTAATGCCGGTATGTCAACTAGTATGTTGGTGACTAAGATGCAAAAAGCAGCCCAAGC
GCGTGACCTTGAAGTTTCTATTTGGGCAGTTCCTGTGAGTGAAGCTGACAACGAAGTGGCTGCTAATAGCATTGACGTTC
TCTTGTTAGGTCCACAAGTCAAATTCTTACTAAAGGATTTTAAAGATAAGTTTGAACCAGATATTAAAGTAGATGCCATT
AACATGGCTGATTATGGTCTTATGAATAGTGAAAAAGTCCTTGAAACAGCCCTAGCGATGATGGAGGAGTAG

Upstream 100 bases:

>100_bases
GAGCTTCAGCAAGCGTTACTAGAAGAACCAAATTATCCCAAATTCATGACAGTGTTTACCCCACTTATTCATGATTAAAA
CGATTAAAAGGAGAAACCTT

Downstream 100 bases:

>100_bases
GCATGACGGGACCATCCAACTTAGAAGCTATTATGGGCTTAATCATGTATGGAGGCGAAGCCAAGGGCAAGGCCGTAGAG
GCTATTCATGCAGCACGAGA

Product: PTS system, cellobiose-specific IIB component

Products: NA

Alternate protein names: EIIB-Cel; PTS system cellobiose-specific EIIB component [H]

Number of amino acids: Translated: 103; Mature: 103

Protein sequence:

>103_residues
MKSIMLVCNAGMSTSMLVTKMQKAAQARDLEVSIWAVPVSEADNEVAANSIDVLLLGPQVKFLLKDFKDKFEPDIKVDAI
NMADYGLMNSEKVLETALAMMEE

Sequences:

>Translated_103_residues
MKSIMLVCNAGMSTSMLVTKMQKAAQARDLEVSIWAVPVSEADNEVAANSIDVLLLGPQVKFLLKDFKDKFEPDIKVDAI
NMADYGLMNSEKVLETALAMMEE
>Mature_103_residues
MKSIMLVCNAGMSTSMLVTKMQKAAQARDLEVSIWAVPVSEADNEVAANSIDVLLLGPQVKFLLKDFKDKFEPDIKVDAI
NMADYGLMNSEKVLETALAMMEE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1440

COG function: function code G; Phosphotransferase system cellobiose-specific component IIB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788034, Length=93, Percent_Identity=37.6344086021505, Blast_Score=60, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014350
- InterPro:   IPR003501
- InterPro:   IPR013012 [H]

Pfam domain/function: PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 11341; Mature: 11341

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS51100 PTS_EIIB_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
8.7 %Met     (Translated Protein)
9.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
8.7 %Met     (Mature Protein)
9.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIMLVCNAGMSTSMLVTKMQKAAQARDLEVSIWAVPVSEADNEVAANSIDVLLLGPQV
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCHH
KFLLKDFKDKFEPDIKVDAINMADYGLMNSEKVLETALAMMEE
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKSIMLVCNAGMSTSMLVTKMQKAAQARDLEVSIWAVPVSEADNEVAANSIDVLLLGPQV
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCHH
KFLLKDFKDKFEPDIKVDAINMADYGLMNSEKVLETALAMMEE
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407820 [H]