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The map label for this gene is flgJ [H]

Identifier: 19746219

GI number: 19746219

Start: 1046900

End: 1047457

Strand: Reverse

Name: flgJ [H]

Synonym: spyM18_1244

Alternate gene names: 19746219

Gene position: 1047457-1046900 (Counterclockwise)

Preceding gene: 19746220

Following gene: 19746218

Centisome position: 55.27

GC content: 42.83

Gene sequence:

>558_bases
ATGACCTTTTTAGATAACATTAAGCAAGGCTGCTTAGATGGCTGGACTAAGTACAAAATCTTGCCATCCTTGACCGCAGC
ACAAGCAATCTTAGAGAGCGGTTGGGGCAAACATGCCCCACATAACGCTTTATTTGGGATTAAGGCTGATAGCTCTTGGA
CTGGTAAGTCATTTGATACTAAGACGCAAGAGGAGTATCAACCAGGAGTTGTGACGGATATTGTAGACCGCTTTAGGGCT
TACGGTAGTTGGGATGAGTCAATTCTTGATCACGGCAAATTTTTAAACGACAATCCACGCTATAAGGCAGTCGTTGGTGA
GACTGACTATAAAAAGGCTTGCCATGCTATTAAAGATGCAGGGTATGCTACTGCGAGTGGATATGCGGAGCTACTTATCC
AAATTATCAAGGAGAATGGCTTGCAGTTTTGGGATGCCGAAGTCTTAAAAAGTAATAAGGAGGAAACAATGACAACCGCA
AATGAGATTGTAAAATACTGTGTCGACCTTGCCAATTCAGGCATGGGAGTTGATAAAGATGGTGCTTATGGAACTTAG

Upstream 100 bases:

>100_bases
CAATCACACGATTAGCGTTAATACTAGCAATCGCAATACTGTATGTGCCGATGTCTGTGGTTGCTCTTATCTTTTATCCG
TTTTTGGATGAGGAGGACAG

Downstream 100 bases:

>100_bases
TAAATTGGGTTCCTAACCCCGAGAATTGCTGGGACTCCCTTAGAGCATTGTAAGCCACAACGTGTCTGGCAACAGAGAGC
GTGACGGTTAAAAAATTACA

Product: hypothetical protein

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MTFLDNIKQGCLDGWTKYKILPSLTAAQAILESGWGKHAPHNALFGIKADSSWTGKSFDTKTQEEYQPGVVTDIVDRFRA
YGSWDESILDHGKFLNDNPRYKAVVGETDYKKACHAIKDAGYATASGYAELLIQIIKENGLQFWDAEVLKSNKEETMTTA
NEIVKYCVDLANSGMGVDKDGAYGT

Sequences:

>Translated_185_residues
MTFLDNIKQGCLDGWTKYKILPSLTAAQAILESGWGKHAPHNALFGIKADSSWTGKSFDTKTQEEYQPGVVTDIVDRFRA
YGSWDESILDHGKFLNDNPRYKAVVGETDYKKACHAIKDAGYATASGYAELLIQIIKENGLQFWDAEVLKSNKEETMTTA
NEIVKYCVDLANSGMGVDKDGAYGT
>Mature_184_residues
TFLDNIKQGCLDGWTKYKILPSLTAAQAILESGWGKHAPHNALFGIKADSSWTGKSFDTKTQEEYQPGVVTDIVDRFRAY
GSWDESILDHGKFLNDNPRYKAVVGETDYKKACHAIKDAGYATASGYAELLIQIIKENGLQFWDAEVLKSNKEETMTTAN
EIVKYCVDLANSGMGVDKDGAYGT

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=122, Percent_Identity=37.7049180327869, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 20459; Mature: 20328

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFLDNIKQGCLDGWTKYKILPSLTAAQAILESGWGKHAPHNALFGIKADSSWTGKSFDT
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCC
KTQEEYQPGVVTDIVDRFRAYGSWDESILDHGKFLNDNPRYKAVVGETDYKKACHAIKDA
CCHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHC
GYATASGYAELLIQIIKENGLQFWDAEVLKSNKEETMTTANEIVKYCVDLANSGMGVDKD
CCCCHHHHHHHHHHHHHHCCCCEEHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GAYGT
CCCCC
>Mature Secondary Structure 
TFLDNIKQGCLDGWTKYKILPSLTAAQAILESGWGKHAPHNALFGIKADSSWTGKSFDT
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCC
KTQEEYQPGVVTDIVDRFRAYGSWDESILDHGKFLNDNPRYKAVVGETDYKKACHAIKDA
CCHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHC
GYATASGYAELLIQIIKENGLQFWDAEVLKSNKEETMTTANEIVKYCVDLANSGMGVDKD
CCCCHHHHHHHHHHHHHHCCCCEEHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GAYGT
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]