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The map label for this gene is femB [H]

Identifier: 19745722

GI number: 19745722

Start: 544190

End: 545416

Strand: Reverse

Name: femB [H]

Synonym: spyM18_0683

Alternate gene names: 19745722

Gene position: 545416-544190 (Counterclockwise)

Preceding gene: 19745723

Following gene: 19745721

Centisome position: 28.78

GC content: 39.69

Gene sequence:

>1227_bases
ATGTATACATATAAAATCGGAATATCTCCTGAAGAACATGATCAGTTTGTCTTAGCACAGCCACAAGCTGGCTTACTTCA
AAGTTCAAAGTGGGGAAAAGTCAAAGATAACTGGAAACACGAACGCATCAGCTTTTATGAAAATGGGGTACAAGTTGCTG
CTGCTGCTTGTTTAATCAGAAAACTTCCTCTTGGTTTCACTATGATTTATATCCCACGTGGGCCAGTCATGGACTACGCC
AATTTTGAACTCCTTGATTTCGTTATTAAAACGCTCAAAACGTTCGGAAAAAGCAAACGAGCGCTTTTTGTCAAAATTGA
TCCAAGTTTAGTCATCAAACAAACTTTAGAAGGAAAAGAAAGCAAAGAAAATGATGTGACACTCTCTATTATTGCTTTTC
TAAAAAAACTTGGTGTCGAATGGTCTGGCAGAACAAAAGAACTCGAAGACACTATTCAACCAAGAATACAAGCCAACATC
TACGCCAAGGATTTTGACTTTGACAGCCTGCCCAAAAAAGCCAAGCAATCCATTCGTACCGCTACCAATAAAGGTGTTAA
TGTCACGATTGGTGGCAGTGAATTGCTAGACGACTTTTCAGCCTTGATGAAAAAAACCGAGAATCGAAAAGGCATTATCC
TTCGAGGCAAATCCTACTATCAAAAATTACTAGATATTTATGCTGGTCAGTCCTACATTACTATGGCTAGTTTAGACTTA
CCTGAACAAAAAAAACTCTTGATCCAACAGTTGGACAAGGCCTTAGCAGAACAAGCACGATTAACAGATAAGAGTAAACC
TAGCAAAGTAGCAGAAAATCAAAAAACCATTGCACGGCTACAAAAAGATTTAACCATTTTAAGCGAGCAGCTCGCTGCAG
GCAAAACCCGCATCCCGCTAGCCGCCACCCTAACCTTGATTTATGGCGAGACTTCTGAAAATCTTTATGCGGGAATGGAC
GATAATTATCGCAATTATCAAGCTCCTCTTTTAACCTGGTATGAAACGGCTAAAGAAGCTTTTAAAAGAGGCTGCCGCTG
GCATAATTTAGGAGGTGTGGAAAATCAACAAGATGGTGGACTCTATCATTTCAAAGCTAGACTCAACCCCACCATCGAAG
AATTTGCAGGTGAATTCAACATTCCTGTCGGCCTTGTTTCAAGCTTAGCCATCTTGACCTATAACTTAAGAAAAAAATTA
AGGAGCACAACTAATGGCACTAATTGA

Upstream 100 bases:

>100_bases
AAAATGGGGTTCCTGAATTGAAAAAAATAGCCAAATACATCACCAAATCCAACAATGACAGTGGTGTGGCACATGCTATT
CGCAAGTGGGTATTAAACTA

Downstream 100 bases:

>100_bases
AATTTCACAAGAACAATTTGACCATTACTGCCACAGTCTCGTCCATCATTCCTTTATCCAAACCTCAGAAATGGCCAGCT
TGATGGCTAAAAGAGGGGCA

Product: peptidoglycan branched peptide synthesis protein, serine/alanine adding enzyme

Products: NA

Alternate protein names: Factor essential for expression of methicillin resistance X [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MYTYKIGISPEEHDQFVLAQPQAGLLQSSKWGKVKDNWKHERISFYENGVQVAAAACLIRKLPLGFTMIYIPRGPVMDYA
NFELLDFVIKTLKTFGKSKRALFVKIDPSLVIKQTLEGKESKENDVTLSIIAFLKKLGVEWSGRTKELEDTIQPRIQANI
YAKDFDFDSLPKKAKQSIRTATNKGVNVTIGGSELLDDFSALMKKTENRKGIILRGKSYYQKLLDIYAGQSYITMASLDL
PEQKKLLIQQLDKALAEQARLTDKSKPSKVAENQKTIARLQKDLTILSEQLAAGKTRIPLAATLTLIYGETSENLYAGMD
DNYRNYQAPLLTWYETAKEAFKRGCRWHNLGGVENQQDGGLYHFKARLNPTIEEFAGEFNIPVGLVSSLAILTYNLRKKL
RSTTNGTN

Sequences:

>Translated_408_residues
MYTYKIGISPEEHDQFVLAQPQAGLLQSSKWGKVKDNWKHERISFYENGVQVAAAACLIRKLPLGFTMIYIPRGPVMDYA
NFELLDFVIKTLKTFGKSKRALFVKIDPSLVIKQTLEGKESKENDVTLSIIAFLKKLGVEWSGRTKELEDTIQPRIQANI
YAKDFDFDSLPKKAKQSIRTATNKGVNVTIGGSELLDDFSALMKKTENRKGIILRGKSYYQKLLDIYAGQSYITMASLDL
PEQKKLLIQQLDKALAEQARLTDKSKPSKVAENQKTIARLQKDLTILSEQLAAGKTRIPLAATLTLIYGETSENLYAGMD
DNYRNYQAPLLTWYETAKEAFKRGCRWHNLGGVENQQDGGLYHFKARLNPTIEEFAGEFNIPVGLVSSLAILTYNLRKKL
RSTTNGTN
>Mature_408_residues
MYTYKIGISPEEHDQFVLAQPQAGLLQSSKWGKVKDNWKHERISFYENGVQVAAAACLIRKLPLGFTMIYIPRGPVMDYA
NFELLDFVIKTLKTFGKSKRALFVKIDPSLVIKQTLEGKESKENDVTLSIIAFLKKLGVEWSGRTKELEDTIQPRIQANI
YAKDFDFDSLPKKAKQSIRTATNKGVNVTIGGSELLDDFSALMKKTENRKGIILRGKSYYQKLLDIYAGQSYITMASLDL
PEQKKLLIQQLDKALAEQARLTDKSKPSKVAENQKTIARLQKDLTILSEQLAAGKTRIPLAATLTLIYGETSENLYAGMD
DNYRNYQAPLLTWYETAKEAFKRGCRWHNLGGVENQQDGGLYHFKARLNPTIEEFAGEFNIPVGLVSSLAILTYNLRKKL
RSTTNGTN

Specific function: Catalyzes the incorporation of the first glycine of the pentaglycine interpeptide bridge, which is characteristic of the S.aureus peptidoglycan. This glycine is added to the epsilon-amino group of the L-lysine of the membrane-bound lipid II intermediate (

COG id: COG2348

COG function: function code V; Uncharacterized protein involved in methicillin resistance

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the femABX family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016181
- InterPro:   IPR003447 [H]

Pfam domain/function: PF02388 FemAB [H]

EC number: NA

Molecular weight: Translated: 46022; Mature: 46022

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTYKIGISPEEHDQFVLAQPQAGLLQSSKWGKVKDNWKHERISFYENGVQVAAAACLIR
CEEEEECCCCCCCCCEEEECCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KLPLGFTMIYIPRGPVMDYANFELLDFVIKTLKTFGKSKRALFVKIDPSLVIKQTLEGKE
HCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCC
SKENDVTLSIIAFLKKLGVEWSGRTKELEDTIQPRIQANIYAKDFDFDSLPKKAKQSIRT
CCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHH
ATNKGVNVTIGGSELLDDFSALMKKTENRKGIILRGKSYYQKLLDIYAGQSYITMASLDL
HCCCCCEEEECHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHCCCCEEEEEECCC
PEQKKLLIQQLDKALAEQARLTDKSKPSKVAENQKTIARLQKDLTILSEQLAAGKTRIPL
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AATLTLIYGETSENLYAGMDDNYRNYQAPLLTWYETAKEAFKRGCRWHNLGGVENQQDGG
EEEEEEEECCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
LYHFKARLNPTIEEFAGEFNIPVGLVSSLAILTYNLRKKLRSTTNGTN
EEEEEECCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MYTYKIGISPEEHDQFVLAQPQAGLLQSSKWGKVKDNWKHERISFYENGVQVAAAACLIR
CEEEEECCCCCCCCCEEEECCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KLPLGFTMIYIPRGPVMDYANFELLDFVIKTLKTFGKSKRALFVKIDPSLVIKQTLEGKE
HCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCC
SKENDVTLSIIAFLKKLGVEWSGRTKELEDTIQPRIQANIYAKDFDFDSLPKKAKQSIRT
CCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHH
ATNKGVNVTIGGSELLDDFSALMKKTENRKGIILRGKSYYQKLLDIYAGQSYITMASLDL
HCCCCCEEEECHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHCCCCEEEEEECCC
PEQKKLLIQQLDKALAEQARLTDKSKPSKVAENQKTIARLQKDLTILSEQLAAGKTRIPL
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AATLTLIYGETSENLYAGMDDNYRNYQAPLLTWYETAKEAFKRGCRWHNLGGVENQQDGG
EEEEEEEECCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
LYHFKARLNPTIEEFAGEFNIPVGLVSSLAILTYNLRKKLRSTTNGTN
EEEEEECCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA