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The map label for this gene is lysS [H]

Identifier: 19745706

GI number: 19745706

Start: 526584

End: 528077

Strand: Reverse

Name: lysS [H]

Synonym: spyM18_0663

Alternate gene names: 19745706

Gene position: 528077-526584 (Counterclockwise)

Preceding gene: 19745708

Following gene: 19745702

Centisome position: 27.87

GC content: 41.9

Gene sequence:

>1494_bases
ATGTCTAATCAACACATCGAAGAATTAAATGACCAACAGATTGTCCGTCGTGAAAAGATGATGGCTCTGGCTGAACAAGG
AATTGATCCTTTCGGTAAACGCTTTGACCGCACAGCCAATTCTGCTGAATTAAAAGAAAAGTATGCTGATAAAACAAAAG
AAGAATTACATGAGTTAAACGAAACAGCTATTGTTGCTGGCCGCTTGATGACAAAACGTGGCAAAGGTAAGGTTGGTTTT
GCTCATCTTCAAGACCGTGAAGGCCAAATCCAACTTTATGTTCGCAAGGACTCTGTTAGCGAAGACAACTATGAAATCTT
CAAAAAAGCCGATTTAGGCGACTTTATCGGCGTTGAAGGCGAAGTCATGCGTACAGACATGGGCGAGTTGTCAATCAAGG
CAACCAAGTTGACTCACCTTTCTAAATCACTTCGTCCTCTTCCTGAAAAATTCCACGGATTAACTGATATTGAAACCATT
TACCGTAAGCGTCACCTTGATTTAATTTCAAATCGTGAAAGCTTTGACCGCTTCGTGACCCGTTCCAAAATGATTTCAGA
AATCCGTCGTTATTTAGACGGTCTTGATTTCCTAGAAGTAGAAACGCCTGTGCTTCATAACGAGGCTGGTGGTGCTGCTG
CTCGTCCTTTCGTTACTCACCATAATGCTCAAAACATTGATATGGTGCTTCGTATCGCAACAGAACTTCATTTGAAACGT
TTGATTGTTGGTGGTATGGAACGTGTCTATGAAATTGGTCGTATCTTCCGTAACGAGGGAATGGATGCTACTCATAATCC
TGAGTTCACTTCTATTGAGGTTTACCAAGCCTATGCAGACTATCTTGATATCATGAACCTGACAGAAGGCATTATCCAAC
ATGCTGCTAAAGCTGTTAAAGGTGATGGCCCTATTGATTATCAAGGAACTGAAATCCGTATCAATGAACCTTTCAAACGT
GTTCACATGGTAGATGCTATCAAGGAAGTGACAGGTGTTGATTTCTGGCCTGAAATGACTGTCGAAGAAGCTATTGCACT
TGCCAAAGAAAAACAGGTCCCACTCGAAAAACACTTCACAAGTGTCGGACATATCATCAATGCTTTCTTCGAAGAATTCG
TTGAAGAAACCTTGGTCCAACCAACCTTTGTGTTTGGTCATCCTGTTGAAGTATCGCCTTTGGCTAAGAAGAATCCAGAA
GATACCCGCTTCACCGACCGCTTCGAACTTTTCATCATGACCAAAGAATACGCCAATGCTTTCACAGAGTTGAATGATCC
CATTGACCAATTATCTCGTTTTGAAGCACAAGCACAAGCTAAAGAACTTGGTGATGACGAAGCAACTGGCATTGACTACG
ACTTCGTTGAAGCTCTTGAATATGGTATGCCGCCAACCGGTGGACTCGGAATCGGGATTGATCGTCTTTGCATGTTATTA
ACAAACACAACAACAATCAGAGATGTGCTTTTATTCCCAACGATGAAACCTTAA

Upstream 100 bases:

>100_bases
CAAAAAAGTGCCCCGCTGCAGTCGCATTTTCCAACGAAATATAGGTGTATTCTAGTCTCTTTTATGATACAATGGAAAGG
AAATCGTATTGGAGGAAAAT

Downstream 100 bases:

>100_bases
CTTTTTTAAGATTTGTATTCATATCTTAAAACCGATAAGTGGCAATATAACATAACGTTTTATTATCTCTAACCAGTCTT
CATTACCATGAAGACTGGTT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MSNQHIEELNDQQIVRREKMMALAEQGIDPFGKRFDRTANSAELKEKYADKTKEELHELNETAIVAGRLMTKRGKGKVGF
AHLQDREGQIQLYVRKDSVSEDNYEIFKKADLGDFIGVEGEVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETI
YRKRHLDLISNRESFDRFVTRSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFVTHHNAQNIDMVLRIATELHLKR
LIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQAYADYLDIMNLTEGIIQHAAKAVKGDGPIDYQGTEIRINEPFKR
VHMVDAIKEVTGVDFWPEMTVEEAIALAKEKQVPLEKHFTSVGHIINAFFEEFVEETLVQPTFVFGHPVEVSPLAKKNPE
DTRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQAQAKELGDDEATGIDYDFVEALEYGMPPTGGLGIGIDRLCMLL
TNTTTIRDVLLFPTMKP

Sequences:

>Translated_497_residues
MSNQHIEELNDQQIVRREKMMALAEQGIDPFGKRFDRTANSAELKEKYADKTKEELHELNETAIVAGRLMTKRGKGKVGF
AHLQDREGQIQLYVRKDSVSEDNYEIFKKADLGDFIGVEGEVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETI
YRKRHLDLISNRESFDRFVTRSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFVTHHNAQNIDMVLRIATELHLKR
LIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQAYADYLDIMNLTEGIIQHAAKAVKGDGPIDYQGTEIRINEPFKR
VHMVDAIKEVTGVDFWPEMTVEEAIALAKEKQVPLEKHFTSVGHIINAFFEEFVEETLVQPTFVFGHPVEVSPLAKKNPE
DTRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQAQAKELGDDEATGIDYDFVEALEYGMPPTGGLGIGIDRLCMLL
TNTTTIRDVLLFPTMKP
>Mature_496_residues
SNQHIEELNDQQIVRREKMMALAEQGIDPFGKRFDRTANSAELKEKYADKTKEELHELNETAIVAGRLMTKRGKGKVGFA
HLQDREGQIQLYVRKDSVSEDNYEIFKKADLGDFIGVEGEVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETIY
RKRHLDLISNRESFDRFVTRSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFVTHHNAQNIDMVLRIATELHLKRL
IVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQAYADYLDIMNLTEGIIQHAAKAVKGDGPIDYQGTEIRINEPFKRV
HMVDAIKEVTGVDFWPEMTVEEAIALAKEKQVPLEKHFTSVGHIINAFFEEFVEETLVQPTFVFGHPVEVSPLAKKNPED
TRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQAQAKELGDDEATGIDYDFVEALEYGMPPTGGLGIGIDRLCMLLT
NTTTIRDVLLFPTMKP

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=508, Percent_Identity=42.1259842519685, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI194272210, Length=508, Percent_Identity=42.1259842519685, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI40789249, Length=288, Percent_Identity=24.3055555555556, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1789256, Length=510, Percent_Identity=49.4117647058824, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1790571, Length=503, Percent_Identity=49.7017892644135, Blast_Score=467, Evalue=1e-133,
Organism=Escherichia coli, GI87082379, Length=324, Percent_Identity=29.9382716049383, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1788173, Length=255, Percent_Identity=26.6666666666667, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=488, Percent_Identity=44.2622950819672, Blast_Score=391, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17535927, Length=488, Percent_Identity=44.2622950819672, Blast_Score=391, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI71994340, Length=488, Percent_Identity=44.2622950819672, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI32566633, Length=297, Percent_Identity=26.2626262626263, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=492, Percent_Identity=44.9186991869919, Blast_Score=392, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324256, Length=504, Percent_Identity=33.9285714285714, Blast_Score=228, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6325153, Length=292, Percent_Identity=26.7123287671233, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24640849, Length=486, Percent_Identity=44.4444444444444, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24640851, Length=488, Percent_Identity=44.2622950819672, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24584738, Length=310, Percent_Identity=25.1612903225806, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 56648; Mature: 56517

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQHIEELNDQQIVRREKMMALAEQGIDPFGKRFDRTANSAELKEKYADKTKEELHELN
CCCCHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ETAIVAGRLMTKRGKGKVGFAHLQDREGQIQLYVRKDSVSEDNYEIFKKADLGDFIGVEG
HHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHCCCCHHCCCCC
EVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETIYRKRHLDLISNRESFDRFVT
CEEECCCCHHEEEHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
RSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFVTHHNAQNIDMVLRIATELHLKR
HHHHHHHHHHHHCCCCEEECCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQAYADYLDIMNLTEGIIQHAAKAVK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDGPIDYQGTEIRINEPFKRVHMVDAIKEVTGVDFWPEMTVEEAIALAKEKQVPLEKHFT
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
SVGHIINAFFEEFVEETLVQPTFVFGHPVEVSPLAKKNPEDTRFTDRFELFIMTKEYANA
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEHHHHHH
FTELNDPIDQLSRFEAQAQAKELGDDEATGIDYDFVEALEYGMPPTGGLGIGIDRLCMLL
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
TNTTTIRDVLLFPTMKP
HCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
SNQHIEELNDQQIVRREKMMALAEQGIDPFGKRFDRTANSAELKEKYADKTKEELHELN
CCCHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ETAIVAGRLMTKRGKGKVGFAHLQDREGQIQLYVRKDSVSEDNYEIFKKADLGDFIGVEG
HHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHCCCCHHCCCCC
EVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETIYRKRHLDLISNRESFDRFVT
CEEECCCCHHEEEHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
RSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFVTHHNAQNIDMVLRIATELHLKR
HHHHHHHHHHHHCCCCEEECCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQAYADYLDIMNLTEGIIQHAAKAVK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDGPIDYQGTEIRINEPFKRVHMVDAIKEVTGVDFWPEMTVEEAIALAKEKQVPLEKHFT
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
SVGHIINAFFEEFVEETLVQPTFVFGHPVEVSPLAKKNPEDTRFTDRFELFIMTKEYANA
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEHHHHHH
FTELNDPIDQLSRFEAQAQAKELGDDEATGIDYDFVEALEYGMPPTGGLGIGIDRLCMLL
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
TNTTTIRDVLLFPTMKP
HCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]