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The map label for this gene is tnpA [C]

Identifier: 19745683

GI number: 19745683

Start: 504159

End: 504962

Strand: Reverse

Name: tnpA [C]

Synonym: spyM18_0634

Alternate gene names: 19745683

Gene position: 504962-504159 (Counterclockwise)

Preceding gene: 19745684

Following gene: 19745670

Centisome position: 26.65

GC content: 32.96

Gene sequence:

>804_bases
TTGTGTCGTGTTTTACGTGTCAATCGCTCCACTTACTATAAATTTCTAAAACATAAGCCCTCAAAAAGAGAATTGGATAA
TCAAATTTATAGAAAACAAATACTTGAGATTTATACCAAAGCAAACAAAAGACTTAGTGTGAAGTCTATCAAGGTCATTC
TTCAAAGAGACTACGACACAAAAATCTCTGAAGGAAGAATTTACCGTCTAATGAAGAATATGGCGCTCCCTAAAATGGCT
ACCGTTAAGCCAAAAACAGCTCTTAAAAAGACTCAAAAAACGTATCCTCAAAACTTACTCAACCAGAAATTTTATCCTGA
CAAACCTAATCAAGTATGGTCTACTGACTTCACCTACATTTCTATTGGATATAAGAAATATGTCTATCTCTGCGCAATAC
TTGATCTCTATTCTAGAAAATGTATTGCTTGGAAATTGAGTCATCATATGGATGTAAAGTTAGCATGTGACACTCTAGAA
TTAGCTCTTAATAAAAGAAAGATTGAAGGAACACTTCTCTTTCATTCCGACCAAGGGTCACAATTTAAGGCCAGGGAATT
TAGAAAAATAATTGATGACAACACTATCATGCATTCTTTTTCTAAACCTGGATATCCTTATGATAATGCCGTAACTGAAG
CATTTTTCAAGTATTTAAAGCATAGACAAATCAACCGAAAAAAGTATCAAAATATCAAACAGGTTCAATTAGATTGCTTT
GAATACATTGAGAATTTTTATAACAATTACAACCCACATACGGCTAATCTAGGACTAACCCCTAATCAGAAAGAAGAAAA
TTAG

Upstream 100 bases:

>100_bases
AGAACCTTATCCTAAAAAAAGCAAGTGTCATATTCATGCAAAACTTAAAGTAAGACTCCTCGCTGTCTATCGGTTACGCT
CTGAACACGCCACAACAACC

Downstream 100 bases:

>100_bases
TTTAACGCAATAAAATAACACGGTTTTCTGTCTACTTATTTGACATTAGTCCAAAATGTACATCCTAATGGTGTCGTTTT
TTTTATTTCACTAGACATTC

Product: transposase TnpA fragment

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MCRVLRVNRSTYYKFLKHKPSKRELDNQIYRKQILEIYTKANKRLSVKSIKVILQRDYDTKISEGRIYRLMKNMALPKMA
TVKPKTALKKTQKTYPQNLLNQKFYPDKPNQVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHHMDVKLACDTLE
LALNKRKIEGTLLFHSDQGSQFKAREFRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINRKKYQNIKQVQLDCF
EYIENFYNNYNPHTANLGLTPNQKEEN

Sequences:

>Translated_267_residues
MCRVLRVNRSTYYKFLKHKPSKRELDNQIYRKQILEIYTKANKRLSVKSIKVILQRDYDTKISEGRIYRLMKNMALPKMA
TVKPKTALKKTQKTYPQNLLNQKFYPDKPNQVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHHMDVKLACDTLE
LALNKRKIEGTLLFHSDQGSQFKAREFRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINRKKYQNIKQVQLDCF
EYIENFYNNYNPHTANLGLTPNQKEEN
>Mature_267_residues
MCRVLRVNRSTYYKFLKHKPSKRELDNQIYRKQILEIYTKANKRLSVKSIKVILQRDYDTKISEGRIYRLMKNMALPKMA
TVKPKTALKKTQKTYPQNLLNQKFYPDKPNQVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHHMDVKLACDTLE
LALNKRKIEGTLLFHSDQGSQFKAREFRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINRKKYQNIKQVQLDCF
EYIENFYNNYNPHTANLGLTPNQKEEN

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=220, Percent_Identity=29.5454545454545, Blast_Score=111, Evalue=7e-25,
Organism=Escherichia coli, GI1788405, Length=274, Percent_Identity=30.2919708029197, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI1787263, Length=274, Percent_Identity=30.2919708029197, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI1786753, Length=274, Percent_Identity=30.2919708029197, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI1786570, Length=274, Percent_Identity=30.2919708029197, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI1786490, Length=274, Percent_Identity=30.2919708029197, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI1789981, Length=263, Percent_Identity=29.6577946768061, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31915; Mature: 31915

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRVLRVNRSTYYKFLKHKPSKRELDNQIYRKQILEIYTKANKRLSVKSIKVILQRDYDT
CCEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
KISEGRIYRLMKNMALPKMATVKPKTALKKTQKTYPQNLLNQKFYPDKPNQVWSTDFTYI
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCEEE
SIGYKKYVYLCAILDLYSRKCIAWKLSHHMDVKLACDTLELALNKRKIEGTLLFHSDQGS
EECHHHHHHHHHHHHHHCCHHEEEECCCCCCEEEEHHHHHHHHHHHEEEEEEEEECCCCC
QFKAREFRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINRKKYQNIKQVQLDCF
CHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYIENFYNNYNPHTANLGLTPNQKEEN
HHHHHHHHCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MCRVLRVNRSTYYKFLKHKPSKRELDNQIYRKQILEIYTKANKRLSVKSIKVILQRDYDT
CCEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
KISEGRIYRLMKNMALPKMATVKPKTALKKTQKTYPQNLLNQKFYPDKPNQVWSTDFTYI
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCEEE
SIGYKKYVYLCAILDLYSRKCIAWKLSHHMDVKLACDTLELALNKRKIEGTLLFHSDQGS
EECHHHHHHHHHHHHHHCCHHEEEECCCCCCEEEEHHHHHHHHHHHEEEEEEEEECCCCC
QFKAREFRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINRKKYQNIKQVQLDCF
CHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYIENFYNNYNPHTANLGLTPNQKEEN
HHHHHHHHCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8387149 [H]