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The map label for this gene is insI1 [C]

Identifier: 19745530

GI number: 19745530

Start: 350145

End: 351170

Strand: Reverse

Name: insI1 [C]

Synonym: spyM18_0426

Alternate gene names: 19745530

Gene position: 351170-350145 (Counterclockwise)

Preceding gene: 19745539

Following gene: 19745514

Centisome position: 18.53

GC content: 42.59

Gene sequence:

>1026_bases
ATGCAAGATTATTATACACCAAAAGGCAAACAATTGACAATCCATGAGCGTCGGCAAATTCAACGCTGGAAGCTGGAAGG
TCGCTCTAATCGTGAAATTGCTCGCTTACTAGGTAAGGCCCCCCAGACCATTCATAATGAAATCAAGCGAGGATTGGTTA
GGCAACAGGTTCGTAAAGGAAAGTTTGAAGCCGTTTATTCTGCTCAACATGCTCAGTTGGTCTATCAAGAAAATCGCAAG
CGTTCTGTTAGACCCATTAGACAGCGTAAGCAAGATAGGGAGGCTATTGAACACTACATGCGTCAGAACTATTCCCCTGA
GATGATAGTTAAAGCTAAGGGAGTTCAAGTCCCTGTATCTACCATCTATTACTGGATTCATCATGGGAAATTAAGTCTTG
GTAAGGAGGCTATGCTTTATCCGAGAAAGGCTAAGCAGGCTAGAAAACATGCCAGTCCTTATTTTAAACCCGCAGGAAAG
AGTATTGAACAACGCCCAGATAGCATTAACCAACGTCTTGAAGCTGGGCATTATGAGATTGATACAGTCATTCTGACGAG
GGCTAAAAATCAATGCTTATTCACCCTAACGGACAGGAAAACACGTCATCAGCTGATTCGATTAATACCGGATAAGTCTG
CTCAAGCTGTCAATAAAGCTCTTACGGGGATTCTAAAAGACTACACCGTCAACTCTATCACGGCAGACAACGGGACAGAA
TTTTCTCGCTTATCAGATGTCTTTCCAGCAAGTGACATCTACTATGCCCATCCCTATGCGTCATGGGAGAGAGGCACTAA
CGAAAACCATAATCGCCTGATTCGCAGATGTCTCCCTAAAGGTACCAAAACAACGACCCCTAAAGAAGTCGCTGCTATTG
AACATTGGATCAATCATTATCCTAAACGACTCTTCAACTACAAGTCGCCAGTAGAAATGTTAAAACTGGCTAACTTCAAC
TTGAAATTTGGCACTGACAACGCATGTTGCCAAAAACTGACGCTTTTAAAAAGTCCATTATTTTAG

Upstream 100 bases:

>100_bases
GAAAAATTCTAAAATGAACTTAGCCACTTTTAAGGTATGAAAAAAGCACCTCTAAGAGGTATACTTGTAGTTCACCACAA
ACACAAGGAAACTCAAAAAG

Downstream 100 bases:

>100_bases
TTTATCGTGTTCGTAAGTATGCTTTAACTCTAACCCTTGAAAGCCCTGTTGTCTAAGGGCTTCATAAATCACCATACAAA
CCGTATTTGAGACATTTAAA

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MQDYYTPKGKQLTIHERRQIQRWKLEGRSNREIARLLGKAPQTIHNEIKRGLVRQQVRKGKFEAVYSAQHAQLVYQENRK
RSVRPIRQRKQDREAIEHYMRQNYSPEMIVKAKGVQVPVSTIYYWIHHGKLSLGKEAMLYPRKAKQARKHASPYFKPAGK
SIEQRPDSINQRLEAGHYEIDTVILTRAKNQCLFTLTDRKTRHQLIRLIPDKSAQAVNKALTGILKDYTVNSITADNGTE
FSRLSDVFPASDIYYAHPYASWERGTNENHNRLIRRCLPKGTKTTTPKEVAAIEHWINHYPKRLFNYKSPVEMLKLANFN
LKFGTDNACCQKLTLLKSPLF

Sequences:

>Translated_341_residues
MQDYYTPKGKQLTIHERRQIQRWKLEGRSNREIARLLGKAPQTIHNEIKRGLVRQQVRKGKFEAVYSAQHAQLVYQENRK
RSVRPIRQRKQDREAIEHYMRQNYSPEMIVKAKGVQVPVSTIYYWIHHGKLSLGKEAMLYPRKAKQARKHASPYFKPAGK
SIEQRPDSINQRLEAGHYEIDTVILTRAKNQCLFTLTDRKTRHQLIRLIPDKSAQAVNKALTGILKDYTVNSITADNGTE
FSRLSDVFPASDIYYAHPYASWERGTNENHNRLIRRCLPKGTKTTTPKEVAAIEHWINHYPKRLFNYKSPVEMLKLANFN
LKFGTDNACCQKLTLLKSPLF
>Mature_341_residues
MQDYYTPKGKQLTIHERRQIQRWKLEGRSNREIARLLGKAPQTIHNEIKRGLVRQQVRKGKFEAVYSAQHAQLVYQENRK
RSVRPIRQRKQDREAIEHYMRQNYSPEMIVKAKGVQVPVSTIYYWIHHGKLSLGKEAMLYPRKAKQARKHASPYFKPAGK
SIEQRPDSINQRLEAGHYEIDTVILTRAKNQCLFTLTDRKTRHQLIRLIPDKSAQAVNKALTGILKDYTVNSITADNGTE
FSRLSDVFPASDIYYAHPYASWERGTNENHNRLIRRCLPKGTKTTTPKEVAAIEHWINHYPKRLFNYKSPVEMLKLANFN
LKFGTDNACCQKLTLLKSPLF

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1790736, Length=336, Percent_Identity=27.9761904761905, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787671, Length=336, Percent_Identity=27.9761904761905, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786450, Length=336, Percent_Identity=27.9761904761905, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 39781; Mature: 39781

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDYYTPKGKQLTIHERRQIQRWKLEGRSNREIARLLGKAPQTIHNEIKRGLVRQQVRKG
CCCCCCCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
KFEAVYSAQHAQLVYQENRKRSVRPIRQRKQDREAIEHYMRQNYSPEMIVKAKGVQVPVS
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHH
TIYYWIHHGKLSLGKEAMLYPRKAKQARKHASPYFKPAGKSIEQRPDSINQRLEAGHYEI
HHHHHHHCCCCCCCCHHHHCCHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHCCCEEE
DTVILTRAKNQCLFTLTDRKTRHQLIRLIPDKSAQAVNKALTGILKDYTVNSITADNGTE
EHEEEEECCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCH
FSRLSDVFPASDIYYAHPYASWERGTNENHNRLIRRCLPKGTKTTTPKEVAAIEHWINHY
HHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
PKRLFNYKSPVEMLKLANFNLKFGTDNACCQKLTLLKSPLF
HHHHHCCCCHHHHHHHHCCEEEECCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQDYYTPKGKQLTIHERRQIQRWKLEGRSNREIARLLGKAPQTIHNEIKRGLVRQQVRKG
CCCCCCCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
KFEAVYSAQHAQLVYQENRKRSVRPIRQRKQDREAIEHYMRQNYSPEMIVKAKGVQVPVS
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHH
TIYYWIHHGKLSLGKEAMLYPRKAKQARKHASPYFKPAGKSIEQRPDSINQRLEAGHYEI
HHHHHHHCCCCCCCCHHHHCCHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHCCCEEE
DTVILTRAKNQCLFTLTDRKTRHQLIRLIPDKSAQAVNKALTGILKDYTVNSITADNGTE
EHEEEEECCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCH
FSRLSDVFPASDIYYAHPYASWERGTNENHNRLIRRCLPKGTKTTTPKEVAAIEHWINHY
HHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
PKRLFNYKSPVEMLKLANFNLKFGTDNACCQKLTLLKSPLF
HHHHHCCCCHHHHHHHHCCEEEECCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8393068 [H]