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The map label for this gene is gidB [H]

Identifier: 19745444

GI number: 19745444

Start: 280727

End: 281440

Strand: Reverse

Name: gidB [H]

Synonym: spyM18_0323

Alternate gene names: 19745444

Gene position: 281440-280727 (Counterclockwise)

Preceding gene: 19745455

Following gene: 19745443

Centisome position: 14.85

GC content: 37.68

Gene sequence:

>714_bases
ATGACACCCCAAGACTTTTACAGAACACTAGAGGAAGATGGTTTTTCTTTATCATCGAAACAAAAAGAACAATTTGACAC
CTACTTCAAATTACTAGTGGAATGGAACACTAAAATTAATTTAACAGCTATTACAGAAGAAAATGAGGTTTACCTCAAGC
ATTTTTATGATTCCATCGCTCCTATTTTACAGGGCTTTTTAGCTAATGAACCAATCAAATTATTGGACATTGGAGCTGGA
GCTGGTTTTCCTAGCCTACCCATGAAAATTCTTTTCCCAAACCTTGAGGTTACCATTATTGATTCTCTTAATAAGCGGAT
TTCTTTTCTGACACTCCTTGCTCAGGAATTGGGCTTGGAAAATGTCCATTTTTTTCATGGTAGAGCAGAAGATTTCGGAC
AGGACAAGGCCTTTCGAGGTCAATTTGATGTCGTTACTGCCAGAGCGGTTGCTCGGATGCAGGTTCTATCTGAATTAACC
ATCCCCTTTTTAAAAATTAGAGGAAAATTAATCGCCTTAAAGGCACAAGCTGCTGACCAAGAGTTAGAAGAAGCTAAGAA
TGCTCTTTGTCTTCTTTTTGGTAAAGTCATTAAAAACCATAGCTACCAATTGCCAAATGGAGATTCTCGCTTCATCACCA
TTGTTGAGAAGAAAAAGGAAACACCTAACAAGTATCCAAGAAAAGCTGGCTTGCCTAACAAAAAACCTCTATAA

Upstream 100 bases:

>100_bases
AATAGCAAGTAATGAGGTGTTATTTCTGATGATTTATTAAATTTTGTTATGGGCTATTTTATGATAAAATGGTAACAACT
ATCAAAAACAGAGGATTCAA

Downstream 100 bases:

>100_bases
AATAGTACCAAAGGCTAAGATGAAACTTAGCTTTTTTAACTTATTTTTGTTATTATAGTAGGAGATATTATGTCTGGAGG
AAACATGAAACGCTCTTTTA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MTPQDFYRTLEEDGFSLSSKQKEQFDTYFKLLVEWNTKINLTAITEENEVYLKHFYDSIAPILQGFLANEPIKLLDIGAG
AGFPSLPMKILFPNLEVTIIDSLNKRISFLTLLAQELGLENVHFFHGRAEDFGQDKAFRGQFDVVTARAVARMQVLSELT
IPFLKIRGKLIALKAQAADQELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKKKETPNKYPRKAGLPNKKPL

Sequences:

>Translated_237_residues
MTPQDFYRTLEEDGFSLSSKQKEQFDTYFKLLVEWNTKINLTAITEENEVYLKHFYDSIAPILQGFLANEPIKLLDIGAG
AGFPSLPMKILFPNLEVTIIDSLNKRISFLTLLAQELGLENVHFFHGRAEDFGQDKAFRGQFDVVTARAVARMQVLSELT
IPFLKIRGKLIALKAQAADQELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKKKETPNKYPRKAGLPNKKPL
>Mature_236_residues
TPQDFYRTLEEDGFSLSSKQKEQFDTYFKLLVEWNTKINLTAITEENEVYLKHFYDSIAPILQGFLANEPIKLLDIGAGA
GFPSLPMKILFPNLEVTIIDSLNKRISFLTLLAQELGLENVHFFHGRAEDFGQDKAFRGQFDVVTARAVARMQVLSELTI
PFLKIRGKLIALKAQAADQELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKKKETPNKYPRKAGLPNKKPL

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=175, Percent_Identity=34.8571428571429, Blast_Score=108, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: 2.1.-.-

Molecular weight: Translated: 26905; Mature: 26774

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPQDFYRTLEEDGFSLSSKQKEQFDTYFKLLVEWNTKINLTAITEENEVYLKHFYDSIA
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEECCEEEEEEEECCCHHHHHHHHHHHH
PILQGFLANEPIKLLDIGAGAGFPSLPMKILFPNLEVTIIDSLNKRISFLTLLAQELGLE
HHHHHHHCCCCEEEEEECCCCCCCCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHCCC
NVHFFHGRAEDFGQDKAFRGQFDVVTARAVARMQVLSELTIPFLKIRGKLIALKAQAADQ
CEEEECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHH
ELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKKKETPNKYPRKAGLPNKKPL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TPQDFYRTLEEDGFSLSSKQKEQFDTYFKLLVEWNTKINLTAITEENEVYLKHFYDSIA
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEECCEEEEEEEECCCHHHHHHHHHHHH
PILQGFLANEPIKLLDIGAGAGFPSLPMKILFPNLEVTIIDSLNKRISFLTLLAQELGLE
HHHHHHHCCCCEEEEEECCCCCCCCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHCCC
NVHFFHGRAEDFGQDKAFRGQFDVVTARAVARMQVLSELTIPFLKIRGKLIALKAQAADQ
CEEEECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHH
ELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKKKETPNKYPRKAGLPNKKPL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]