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The map label for this gene is gsiD [H]

Identifier: 197294778

GI number: 197294778

Start: 773043

End: 773903

Strand: Direct

Name: gsiD [H]

Synonym: PAa_0758

Alternate gene names: 197294778

Gene position: 773043-773903 (Clockwise)

Preceding gene: 197294777

Following gene: 197294779

Centisome position: 87.85

GC content: 24.85

Gene sequence:

>861_bases
ATGAAATTTAAATTTACATTAACGAATTATAAAAAAAAATTGAAAATTTTAATTAAAAATAAAAATATTTTATTTGGTAG
TCTTTTTTTAATTATTTTGTTTTTAACTATTTATTTGTTGCCTTTAACTCCTTTTTATCATAATCCTTATAAATCACCTT
TTAAACGTTTACAAAGTTTTAGTTGGAAACATTGGATGGGAACTGATTCTTCAGGTTATGATTTGTTTAGCCGAGTTTTA
GAAGGAGCAAAAATTTCTTTAACTATTGGTTTGATTGCTGTTTTTTTAAGTAGCTTAGTTGGTAGTTTTTTAGGGATTAT
TTCTGGTTATTTTCGTGGAATTATCGATAGTATCATTAATTTTATCTGTGATATTTTAGTTATTTTTCCAGATATAATTT
TGGCTATCATTATTATGTTTTTTTTACAAAAAAGTAAATTATCCTTGATTATTGTTTTAAGTATTTCAAATATTCCTGGT
TTTATCCGCATCATTAGAGCAAATACTTTAAAATTAAGTAAAAAAGACTTTATCAAAGCTTCAAAAGCTTTGGGTGCGAA
TCATTTTTACATTATTTTTAAACATCTTGTGACACATCTTTATCCCATTATTATCACTAGAATAAGTATTTCTATGGCAA
CTGTTGTTTTAACTATTTCAAGTTTAGGTTTTATTGGATTGGGGCTCGATCCTACTTTGCCAGAATGGGGTAATATTCTA
CATTCTGCAAAAGGTGATATGCGCTATCATCCTCATTTATTTTTTGGACCATTTATTATTATTCTTTTAACTAGTTTATC
TTTTAATTTAATTGCTAAAGGGTTTATTTCTTTTTTTAATCCTAAAAAGAAAATTAGTTAA

Upstream 100 bases:

>100_bases
CTATTTGGAATTTATTTTTAGATTTAATCTATCTTTGGTTAGATCCGAAAATAAATAAAAAAGGATAAAAAAATTATGTT
AGAAAGTACAAAAACGAATA

Downstream 100 bases:

>100_bases
ATGAAAACAAACATAACTGTTTATTGTTGACCGATAAACAGTTTTTTTTATAGTTCGAAAAGATGCAAAAATAAGGAAAT
GTGGGATGCTGAAAAAATTA

Product: ABC-type peptide/nickel transport system permease protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKFKFTLTNYKKKLKILIKNKNILFGSLFLIILFLTIYLLPLTPFYHNPYKSPFKRLQSFSWKHWMGTDSSGYDLFSRVL
EGAKISLTIGLIAVFLSSLVGSFLGIISGYFRGIIDSIINFICDILVIFPDIILAIIIMFFLQKSKLSLIIVLSISNIPG
FIRIIRANTLKLSKKDFIKASKALGANHFYIIFKHLVTHLYPIIITRISISMATVVLTISSLGFIGLGLDPTLPEWGNIL
HSAKGDMRYHPHLFFGPFIIILLTSLSFNLIAKGFISFFNPKKKIS

Sequences:

>Translated_286_residues
MKFKFTLTNYKKKLKILIKNKNILFGSLFLIILFLTIYLLPLTPFYHNPYKSPFKRLQSFSWKHWMGTDSSGYDLFSRVL
EGAKISLTIGLIAVFLSSLVGSFLGIISGYFRGIIDSIINFICDILVIFPDIILAIIIMFFLQKSKLSLIIVLSISNIPG
FIRIIRANTLKLSKKDFIKASKALGANHFYIIFKHLVTHLYPIIITRISISMATVVLTISSLGFIGLGLDPTLPEWGNIL
HSAKGDMRYHPHLFFGPFIIILLTSLSFNLIAKGFISFFNPKKKIS
>Mature_286_residues
MKFKFTLTNYKKKLKILIKNKNILFGSLFLIILFLTIYLLPLTPFYHNPYKSPFKRLQSFSWKHWMGTDSSGYDLFSRVL
EGAKISLTIGLIAVFLSSLVGSFLGIISGYFRGIIDSIINFICDILVIFPDIILAIIIMFFLQKSKLSLIIVLSISNIPG
FIRIIRANTLKLSKKDFIKASKALGANHFYIIFKHLVTHLYPIIITRISISMATVVLTISSLGFIGLGLDPTLPEWGNIL
HSAKGDMRYHPHLFFGPFIIILLTSLSFNLIAKGFISFFNPKKKIS

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1173

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787054, Length=278, Percent_Identity=34.8920863309352, Blast_Score=187, Evalue=8e-49,
Organism=Escherichia coli, GI1787760, Length=276, Percent_Identity=30.7971014492754, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1789964, Length=259, Percent_Identity=30.8880308880309, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787498, Length=293, Percent_Identity=28.3276450511945, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1789889, Length=228, Percent_Identity=25.4385964912281, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788505, Length=241, Percent_Identity=27.3858921161826, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787549, Length=209, Percent_Identity=26.7942583732057, Blast_Score=91, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32418; Mature: 32418

Theoretical pI: Translated: 10.79; Mature: 10.79

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKFTLTNYKKKLKILIKNKNILFGSLFLIILFLTIYLLPLTPFYHNPYKSPFKRLQSF
CEEEEEHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
SWKHWMGTDSSGYDLFSRVLEGAKISLTIGLIAVFLSSLVGSFLGIISGYFRGIIDSIIN
CCHHCCCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FICDILVIFPDIILAIIIMFFLQKSKLSLIIVLSISNIPGFIRIIRANTLKLSKKDFIKA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHEEEEHHHHCCCHHHHHHH
SKALGANHFYIIFKHLVTHLYPIIITRISISMATVVLTISSLGFIGLGLDPTLPEWGNIL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHH
HSAKGDMRYHPHLFFGPFIIILLTSLSFNLIAKGFISFFNPKKKIS
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKFKFTLTNYKKKLKILIKNKNILFGSLFLIILFLTIYLLPLTPFYHNPYKSPFKRLQSF
CEEEEEHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
SWKHWMGTDSSGYDLFSRVLEGAKISLTIGLIAVFLSSLVGSFLGIISGYFRGIIDSIIN
CCHHCCCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FICDILVIFPDIILAIIIMFFLQKSKLSLIIVLSISNIPGFIRIIRANTLKLSKKDFIKA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHEEEEHHHHCCCHHHHHHH
SKALGANHFYIIFKHLVTHLYPIIITRISISMATVVLTISSLGFIGLGLDPTLPEWGNIL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHH
HSAKGDMRYHPHLFFGPFIIILLTSLSFNLIAKGFISFFNPKKKIS
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA