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The map label for this gene is pmbA [H]

Identifier: 197294736

GI number: 197294736

Start: 719585

End: 720805

Strand: Reverse

Name: pmbA [H]

Synonym: PAa_0702

Alternate gene names: 197294736

Gene position: 720805-719585 (Counterclockwise)

Preceding gene: 197294737

Following gene: 197294732

Centisome position: 81.91

GC content: 29.16

Gene sequence:

>1221_bases
TTGGAACAAGGAAAAATAGATGCTTGTGTTCATAGCAATGTTAAATCTTTTATTGTCAGAGGGATTTATCAAAACCAAAA
AACATCTTTTTCGTTAGAAAAACTGGATGATGAAGCTTTTTTAGATGCTTTAAAAAACATGAAAGAAAACTGTCAAAATA
TCACTTCCAAAGAACCAGCCTTAATTTTTGAAGGTCCTGCAACTTATCCTGAAGTTAAAGAAAATAAATTTGATTTTAGT
CAAATCCCTTTAGATCATAAAATACAATTATTAAAAGAATTAGAAAAAACAATTTCTGAAAGTGTTTATTTACAAAAAAT
AGATGATTTAATTTATAAAGAAAGTTTTTCTAAAACTACTTTATTGAATTCTAAAAAAGTTTCTTTGTCACATCAAAATA
GCTGCGCTTATCTATACGCTTCAGGGGTTTTTCAAAAAGACAAAGATATTGAAAGTTTTGGCGAAAGTAATTTAGAAAAA
ACTTTTGATGCCTTTGATCCAGTTAAATTAGGCCGTCAAATTGTTGATACGACTGAAAAAAAAATAGGTGGAGTATCTAT
TTTATCTCAAAAATATCCAGTTGTTTTTTCTAATGAAATGTTTGCTGAACTTTTAGATGGTTTTAGTGATATTTTTTCGG
GAAATAGTGCTTATCGTAATTTAACTAAATTAAAAGATAAAGTTAATCATTTAATTGCGACTCCTATCGTCAATCTTTTC
GATGATCCACTTCATGAAAAAGCTTATTTTCAAGAAAAATTCGATGAAGAATGTGTACCTTGTCAAACTAAACCAATCAT
TTATCAAGGTGCCTTTAAAGGATTTATTCACAATTTAAAAACCGCTCACTTTTTTAAGGAAAAACCTACTGGAAATGGTT
TTGGTGGGGGTATTTCTATGGTTAACTGTTACTTAGAGCCAGGAAAAAATTCTTTTCAAGAATTGATTGCTCCAATTCAA
AATGGTGTCTACATTACTGATTTAATTGGACTTCATGCAGGAATTAAAACTGTTAGCGGTGATTTTAGCCTTCAGGCTAG
TGGTTTTAAAATTGAAGCAGGAAAAATTACTTCTCCTGTTAAAATGATTGTTCTTTCTGGTAATTTCTTTCAAGTATTAA
AAGATATTGTAGGAATTGCTAATGATTTTGAATTTCAAACTTCAGGATTTGGCAGCGCTAGTGTTTGTGTTAGTAGTTTA
AGTATTGCTGGAAAAAATTAA

Upstream 100 bases:

>100_bases
AAACAAAATGAAAACAAATAAATGGCTTGAACAAGGTTTAAAAGAAGGTTTTGATGCCTTAGAAATACTTATTCACGAAA
ACAAAAGCTTTAAATTATCT

Downstream 100 bases:

>100_bases
AATTTATTAATTTTAAAAATAATTTTAAAAAAAGAAAATCTGTTTTGAAATTGAAAATAACTAAATTATTATTAAATATT
TGTCTTTATAAAACTAACAA

Product: Pmba-related protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MEQGKIDACVHSNVKSFIVRGIYQNQKTSFSLEKLDDEAFLDALKNMKENCQNITSKEPALIFEGPATYPEVKENKFDFS
QIPLDHKIQLLKELEKTISESVYLQKIDDLIYKESFSKTTLLNSKKVSLSHQNSCAYLYASGVFQKDKDIESFGESNLEK
TFDAFDPVKLGRQIVDTTEKKIGGVSILSQKYPVVFSNEMFAELLDGFSDIFSGNSAYRNLTKLKDKVNHLIATPIVNLF
DDPLHEKAYFQEKFDEECVPCQTKPIIYQGAFKGFIHNLKTAHFFKEKPTGNGFGGGISMVNCYLEPGKNSFQELIAPIQ
NGVYITDLIGLHAGIKTVSGDFSLQASGFKIEAGKITSPVKMIVLSGNFFQVLKDIVGIANDFEFQTSGFGSASVCVSSL
SIAGKN

Sequences:

>Translated_406_residues
MEQGKIDACVHSNVKSFIVRGIYQNQKTSFSLEKLDDEAFLDALKNMKENCQNITSKEPALIFEGPATYPEVKENKFDFS
QIPLDHKIQLLKELEKTISESVYLQKIDDLIYKESFSKTTLLNSKKVSLSHQNSCAYLYASGVFQKDKDIESFGESNLEK
TFDAFDPVKLGRQIVDTTEKKIGGVSILSQKYPVVFSNEMFAELLDGFSDIFSGNSAYRNLTKLKDKVNHLIATPIVNLF
DDPLHEKAYFQEKFDEECVPCQTKPIIYQGAFKGFIHNLKTAHFFKEKPTGNGFGGGISMVNCYLEPGKNSFQELIAPIQ
NGVYITDLIGLHAGIKTVSGDFSLQASGFKIEAGKITSPVKMIVLSGNFFQVLKDIVGIANDFEFQTSGFGSASVCVSSL
SIAGKN
>Mature_406_residues
MEQGKIDACVHSNVKSFIVRGIYQNQKTSFSLEKLDDEAFLDALKNMKENCQNITSKEPALIFEGPATYPEVKENKFDFS
QIPLDHKIQLLKELEKTISESVYLQKIDDLIYKESFSKTTLLNSKKVSLSHQNSCAYLYASGVFQKDKDIESFGESNLEK
TFDAFDPVKLGRQIVDTTEKKIGGVSILSQKYPVVFSNEMFAELLDGFSDIFSGNSAYRNLTKLKDKVNHLIATPIVNLF
DDPLHEKAYFQEKFDEECVPCQTKPIIYQGAFKGFIHNLKTAHFFKEKPTGNGFGGGISMVNCYLEPGKNSFQELIAPIQ
NGVYITDLIGLHAGIKTVSGDFSLQASGFKIEAGKITSPVKMIVLSGNFFQVLKDIVGIANDFEFQTSGFGSASVCVSSL
SIAGKN

Specific function: May Facilitate The Secretion Of The Peptide Antibiotic Microcin B17 (Mccb17) By Completing Its Maturation. Suppresses The Inhibitory Activity Of The Carbon Storage Regulator (Csra). [C]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=398, Percent_Identity=25.3768844221106, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1789640, Length=243, Percent_Identity=25.9259259259259, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 45125; Mature: 45125

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQGKIDACVHSNVKSFIVRGIYQNQKTSFSLEKLDDEAFLDALKNMKENCQNITSKEPA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCE
LIFEGPATYPEVKENKFDFSQIPLDHKIQLLKELEKTISESVYLQKIDDLIYKESFSKTT
EEEECCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LLNSKKVSLSHQNSCAYLYASGVFQKDKDIESFGESNLEKTFDAFDPVKLGRQIVDTTEK
CCCCCEEECCCCCCEEEEEECCCCCCCCCHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHH
KIGGVSILSQKYPVVFSNEMFAELLDGFSDIFSGNSAYRNLTKLKDKVNHLIATPIVNLF
HHCCHHHHCCCCCEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DDPLHEKAYFQEKFDEECVPCQTKPIIYQGAFKGFIHNLKTAHFFKEKPTGNGFGGGISM
CCHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
VNCYLEPGKNSFQELIAPIQNGVYITDLIGLHAGIKTVSGDFSLQASGFKIEAGKITSPV
EEEEECCCCHHHHHHHHHHHCCEEEEHHHHHHHCHHEECCCCEEEECCEEEECCCCCCCE
KMIVLSGNFFQVLKDIVGIANDFEFQTSGFGSASVCVSSLSIAGKN
EEEEECCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEQGKIDACVHSNVKSFIVRGIYQNQKTSFSLEKLDDEAFLDALKNMKENCQNITSKEPA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCE
LIFEGPATYPEVKENKFDFSQIPLDHKIQLLKELEKTISESVYLQKIDDLIYKESFSKTT
EEEECCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LLNSKKVSLSHQNSCAYLYASGVFQKDKDIESFGESNLEKTFDAFDPVKLGRQIVDTTEK
CCCCCEEECCCCCCEEEEEECCCCCCCCCHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHH
KIGGVSILSQKYPVVFSNEMFAELLDGFSDIFSGNSAYRNLTKLKDKVNHLIATPIVNLF
HHCCHHHHCCCCCEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DDPLHEKAYFQEKFDEECVPCQTKPIIYQGAFKGFIHNLKTAHFFKEKPTGNGFGGGISM
CCHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
VNCYLEPGKNSFQELIAPIQNGVYITDLIGLHAGIKTVSGDFSLQASGFKIEAGKITSPV
EEEEECCCCHHHHHHHHHHHCCEEEEHHHHHHHCHHEECCCCEEEECCEEEECCCCCCCE
KMIVLSGNFFQVLKDIVGIANDFEFQTSGFGSASVCVSSLSIAGKN
EEEEECCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]