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The map label for this gene is glnS [H]

Identifier: 197294728

GI number: 197294728

Start: 705844

End: 707475

Strand: Reverse

Name: glnS [H]

Synonym: PAa_0694

Alternate gene names: 197294728

Gene position: 707475-705844 (Counterclockwise)

Preceding gene: 197294729

Following gene: 197294727

Centisome position: 80.4

GC content: 30.33

Gene sequence:

>1632_bases
ATGCAAAAACTAAAAGAAACAAAACTAAACTCTAATTTTATTAAAACTATTATCCAAAAAGATTTAGAAGAAAATAAATG
TAGACAAATTATCACACGTTTTCCGCCCGAACCTAATGGATTTTTGCATTTAGGACACGCAAGAGCTATTATTATTAATT
TTGAATTAGCTAAGTTTTTTAACGGTAAAACTTATTTGCGTTATGATGACACTAACCCAAACAACGAAAAACAAGTTTAT
GTTGATGCTATTTTGCAAGATATAACATGGCTAGGTTATAAACCTGATAAAATTACGTTTGCTTCTGATTATTTTGATCA
AATGTTTGAAAAAGCCCTTTTTTTGATTCAGCAAGGACACGCTTATGTAGATGATTTATCTGCTGAAGAGATTACTAAAT
TAAGAGGTAATTTATTAGAAAAAGGAACAAATTCACGTTGTCGCGAACGTAGCATAGAAGATAACAAAAAACTTTTTATC
GAAATGAAAGAAGGAAAATTTGCTGAAGGCAGTAAGGTTTTGCGAGCCAAAATTGACATGTCAAGTCCTAATCTTAATTT
AAGAGACCCTGTTCTTTATCGCATTCTTTCAGCTCGTACTTTAAACAAAAAACATTGGCATATTTTCCCTACTTATGATT
ACGCTCATCCTTTAGAAGATTCTTTTGAAAAAATTAGTCATTCTTTATGCTCTTTAGAATTTGAAGATCACAGACCTTTA
TATGATTGGGTTATTCAAAAAACTCAAGTTTCTTATATTCCGCGTCAAATTGAATTTGGAAGACTTAATTTAGCTCAAAC
TTTAATGAGTAAGAGACATCTAAAAAGTTTGGTGGAAGAAAAAAAGGTTTTTGGTTGGGATGACCCTCGCATGCCTACTT
TATCAGGAATCAGAAAAAAAGGCTACACGCCCCAAGCCATTAAAAATTTTATTTTAGAAACAGGTTTATCAAAAGTTAAT
TCCACGGTCAAAAAAGACATGTTAGATGCATTCGTTAGAAATGATTTGCGTCCTCAAGCTTTTCCAAGGATGGCAATTAC
AAATCCTTTAAAAGTAACTATTATTAATTATCCAGAAAATCAAATAGAAAAATTAACGGCCCCTTTTTTTCCGAAAGAAC
ACAATGAAGAAAAAACAAGAGAAATTTATTTTTCCCGTCATTTATATATTGAAAAAGATGATTTTTCCCTTGTCAAGCCA
GACCCTCAATATAAAAGATTAGTTTTAGGTGAAGAAGTACGTTTATTTCATGCTTATTTCATTAAAGCTTGTGATGTTGT
TAAAAACGTTCAAAGAGAAGTTATTGAAGTGATTGCAACTTATGATCCTCAAACCAAAAGCGGTAGTGGCTTTAAAGGTC
GTAGGCCCAACGGAACTATTCATTTTGTGGAAAGTACAACAGCCTTAGAAGCTCATTTTCATTTATTTGAAGAACTTTTA
ACAGAAAACGGTTCTTTTAATCACAATTCTTATCAAATCCAAAAAGGTTTTGTTGAAAAAAGTTTAGAAGCTGAAAATCT
TAAAAACCCAATCCAATTTTTAAGACAAGGTTTTTTTGTTTTAGAAAAAAAAGCAGATAAAAAATTACATTTTAATAAAA
TAGTAAGTTTAAAAGTAAGTAATTTGAAATAA

Upstream 100 bases:

>100_bases
GATTCTTATTTATTTAGTTTCTTTGATAAAATATTTATATAGAGTTTTTTTATTTAAAAATTATTTTTAAAATTTAAAAA
AAAGAAACAGGATTGTAAAT

Downstream 100 bases:

>100_bases
AAAGGTTCAATAATGTCATCTAAAATAGATAGTTGGTTAAAAAAATTAAATAAAAATCAAGTAGAAGCGGCTACCTCCAA
ATCTTTGGCTACTTATTTAT

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS [H]

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MQKLKETKLNSNFIKTIIQKDLEENKCRQIITRFPPEPNGFLHLGHARAIIINFELAKFFNGKTYLRYDDTNPNNEKQVY
VDAILQDITWLGYKPDKITFASDYFDQMFEKALFLIQQGHAYVDDLSAEEITKLRGNLLEKGTNSRCRERSIEDNKKLFI
EMKEGKFAEGSKVLRAKIDMSSPNLNLRDPVLYRILSARTLNKKHWHIFPTYDYAHPLEDSFEKISHSLCSLEFEDHRPL
YDWVIQKTQVSYIPRQIEFGRLNLAQTLMSKRHLKSLVEEKKVFGWDDPRMPTLSGIRKKGYTPQAIKNFILETGLSKVN
STVKKDMLDAFVRNDLRPQAFPRMAITNPLKVTIINYPENQIEKLTAPFFPKEHNEEKTREIYFSRHLYIEKDDFSLVKP
DPQYKRLVLGEEVRLFHAYFIKACDVVKNVQREVIEVIATYDPQTKSGSGFKGRRPNGTIHFVESTTALEAHFHLFEELL
TENGSFNHNSYQIQKGFVEKSLEAENLKNPIQFLRQGFFVLEKKADKKLHFNKIVSLKVSNLK

Sequences:

>Translated_543_residues
MQKLKETKLNSNFIKTIIQKDLEENKCRQIITRFPPEPNGFLHLGHARAIIINFELAKFFNGKTYLRYDDTNPNNEKQVY
VDAILQDITWLGYKPDKITFASDYFDQMFEKALFLIQQGHAYVDDLSAEEITKLRGNLLEKGTNSRCRERSIEDNKKLFI
EMKEGKFAEGSKVLRAKIDMSSPNLNLRDPVLYRILSARTLNKKHWHIFPTYDYAHPLEDSFEKISHSLCSLEFEDHRPL
YDWVIQKTQVSYIPRQIEFGRLNLAQTLMSKRHLKSLVEEKKVFGWDDPRMPTLSGIRKKGYTPQAIKNFILETGLSKVN
STVKKDMLDAFVRNDLRPQAFPRMAITNPLKVTIINYPENQIEKLTAPFFPKEHNEEKTREIYFSRHLYIEKDDFSLVKP
DPQYKRLVLGEEVRLFHAYFIKACDVVKNVQREVIEVIATYDPQTKSGSGFKGRRPNGTIHFVESTTALEAHFHLFEELL
TENGSFNHNSYQIQKGFVEKSLEAENLKNPIQFLRQGFFVLEKKADKKLHFNKIVSLKVSNLK
>Mature_543_residues
MQKLKETKLNSNFIKTIIQKDLEENKCRQIITRFPPEPNGFLHLGHARAIIINFELAKFFNGKTYLRYDDTNPNNEKQVY
VDAILQDITWLGYKPDKITFASDYFDQMFEKALFLIQQGHAYVDDLSAEEITKLRGNLLEKGTNSRCRERSIEDNKKLFI
EMKEGKFAEGSKVLRAKIDMSSPNLNLRDPVLYRILSARTLNKKHWHIFPTYDYAHPLEDSFEKISHSLCSLEFEDHRPL
YDWVIQKTQVSYIPRQIEFGRLNLAQTLMSKRHLKSLVEEKKVFGWDDPRMPTLSGIRKKGYTPQAIKNFILETGLSKVN
STVKKDMLDAFVRNDLRPQAFPRMAITNPLKVTIINYPENQIEKLTAPFFPKEHNEEKTREIYFSRHLYIEKDDFSLVKP
DPQYKRLVLGEEVRLFHAYFIKACDVVKNVQREVIEVIATYDPQTKSGSGFKGRRPNGTIHFVESTTALEAHFHLFEELL
TENGSFNHNSYQIQKGFVEKSLEAENLKNPIQFLRQGFFVLEKKADKKLHFNKIVSLKVSNLK

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4826960, Length=530, Percent_Identity=37.7358490566038, Blast_Score=316, Evalue=3e-86,
Organism=Homo sapiens, GI62241042, Length=509, Percent_Identity=33.3988212180747, Blast_Score=249, Evalue=3e-66,
Organism=Escherichia coli, GI1786895, Length=543, Percent_Identity=43.646408839779, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1788743, Length=330, Percent_Identity=24.2424242424242, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17543332, Length=528, Percent_Identity=36.1742424242424, Blast_Score=305, Evalue=5e-83,
Organism=Caenorhabditis elegans, GI17511007, Length=374, Percent_Identity=32.620320855615, Blast_Score=210, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6324742, Length=569, Percent_Identity=34.7978910369069, Blast_Score=317, Evalue=3e-87,
Organism=Saccharomyces cerevisiae, GI6321192, Length=392, Percent_Identity=35.4591836734694, Blast_Score=273, Evalue=4e-74,
Organism=Saccharomyces cerevisiae, GI6324540, Length=325, Percent_Identity=23.6923076923077, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17864488, Length=530, Percent_Identity=38.3018867924528, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24649466, Length=382, Percent_Identity=35.6020942408377, Blast_Score=230, Evalue=2e-60,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.18 [H]

Molecular weight: Translated: 63483; Mature: 63483

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLKETKLNSNFIKTIIQKDLEENKCRQIITRFPPEPNGFLHLGHARAIIINFELAKFF
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEEEHHHHH
NGKTYLRYDDTNPNNEKQVYVDAILQDITWLGYKPDKITFASDYFDQMFEKALFLIQQGH
CCCEEEEECCCCCCCCCEEHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCC
AYVDDLSAEEITKLRGNLLEKGTNSRCRERSIEDNKKLFIEMKEGKFAEGSKVLRAKIDM
HHHHCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEEECCCCCCCCCCEEEEEEEC
SSPNLNLRDPVLYRILSARTLNKKHWHIFPTYDYAHPLEDSFEKISHSLCSLEFEDHRPL
CCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCH
YDWVIQKTQVSYIPRQIEFGRLNLAQTLMSKRHLKSLVEEKKVFGWDDPRMPTLSGIRKK
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHC
GYTPQAIKNFILETGLSKVNSTVKKDMLDAFVRNDLRPQAFPRMAITNPLKVTIINYPEN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEEEEECCHH
QIEKLTAPFFPKEHNEEKTREIYFSRHLYIEKDDFSLVKPDPQYKRLVLGEEVRLFHAYF
HHHHHCCCCCCCCCCCHHHHHHHEEEEEEEECCCCCEECCCCHHHHHHHCHHHHHHHHHH
IKACDVVKNVQREVIEVIATYDPQTKSGSGFKGRRPNGTIHFVESTTALEAHFHLFEELL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHH
TENGSFNHNSYQIQKGFVEKSLEAENLKNPIQFLRQGFFVLEKKADKKLHFNKIVSLKVS
HCCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHCCHHEEEHHCCCCEEHHHEEEEEEC
NLK
CCC
>Mature Secondary Structure
MQKLKETKLNSNFIKTIIQKDLEENKCRQIITRFPPEPNGFLHLGHARAIIINFELAKFF
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEEEHHHHH
NGKTYLRYDDTNPNNEKQVYVDAILQDITWLGYKPDKITFASDYFDQMFEKALFLIQQGH
CCCEEEEECCCCCCCCCEEHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCC
AYVDDLSAEEITKLRGNLLEKGTNSRCRERSIEDNKKLFIEMKEGKFAEGSKVLRAKIDM
HHHHCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEEECCCCCCCCCCEEEEEEEC
SSPNLNLRDPVLYRILSARTLNKKHWHIFPTYDYAHPLEDSFEKISHSLCSLEFEDHRPL
CCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCH
YDWVIQKTQVSYIPRQIEFGRLNLAQTLMSKRHLKSLVEEKKVFGWDDPRMPTLSGIRKK
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHC
GYTPQAIKNFILETGLSKVNSTVKKDMLDAFVRNDLRPQAFPRMAITNPLKVTIINYPEN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEEEEECCHH
QIEKLTAPFFPKEHNEEKTREIYFSRHLYIEKDDFSLVKPDPQYKRLVLGEEVRLFHAYF
HHHHHCCCCCCCCCCCHHHHHHHEEEEEEEECCCCCEECCCCHHHHHHHCHHHHHHHHHH
IKACDVVKNVQREVIEVIATYDPQTKSGSGFKGRRPNGTIHFVESTTALEAHFHLFEELL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHH
TENGSFNHNSYQIQKGFVEKSLEAENLKNPIQFLRQGFFVLEKKADKKLHFNKIVSLKVS
HCCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHCCHHEEEHHCCCCEEHHHEEEEEEC
NLK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA