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The map label for this gene is pnp

Identifier: 197294708

GI number: 197294708

Start: 675962

End: 678094

Strand: Reverse

Name: pnp

Synonym: PAa_0674

Alternate gene names: 197294708

Gene position: 678094-675962 (Counterclockwise)

Preceding gene: 197294709

Following gene: 197294707

Centisome position: 77.06

GC content: 32.49

Gene sequence:

>2133_bases
GTGTTAAAGCAAGTTTTTGAAACCACTGATTTAAAAAATTCTTTCCAAGTAGAAATAGGAACTTATGCAAGAAACGTTGA
CCCTTCTGTTTTAATTCGTTTTCAGGATACAGTTGTTTTGACAACTACAGTTTTTAGTAATAAGAAAAATAATTTTGATT
TCCTCCCTTTAACTGTGATTTATCAAGAAAAATTTTATGCTGCTGGAAAAATTCCTGGCAGTTTTTTGAGAAGAGAAGGT
CGTTCAACTGACCATGAAATTTTATCTTCTCGTTTAATTGATCGTTCTTTAAGACCGTTGTTTCCCAAGGAATTTAGACA
AGAAATACAAGTGATTAATACAGTTTTAAGTTCAAATCCTGATTTTAAAAGCGAAATTGCTTCTATTCTTGGCAGTTCTT
TGTCGCTTCTTATTTCTGAAATCCCCTTTTTTGAGCCTGTTGCAGGCGTTTATGTTGCCTATATTCAAAATCAATTCGTT
ATTAATCCTAACGCCCAACAATTAACCAATTCCCCTTTACATTTATTAGTAGCAGGAACCAAAAAAAATGTTGTTATGAT
TGAAGCCCATGCTTCCGAGGTTTCAGAAGAAATGTTTTTAGAAGCCATTGTTTTTGCTCACGAATACATTAAAAAATTAT
GTTTATTTCAAGAGGATGTTCAAAAAAAAGTTGGTCAAACTAAAAAACTAATTGATTTAGACGCTGAATTGCAGTCATTG
GAAAAAGAATTTAACGAACAATATCACGAACAAACAAGAACCTTGGTTGCGAATGTTTTTGAACAAGATCAAAAAAATGA
TTTGCAAGTTTTCAAAAAAAAAATTCTAGCCAAAGCACAACAAAAAGCTTTTGTTAAAACAATTGACCAAATTACTTTTT
TTGACGTCGAAGAACAAAAAAATTATTTATTGTTAATTGAAAACTTGTTTCAAAAATTATTTAACCAAGAAATGAGAAAT
TATATTATCAAAAACAAAAAACGACCTGATAAAAGAACTTTAGAAGAGGTTAGAAACCTTGATTCACAAATTGATTTGTT
ACCTCGACCCCACGGATCTGCTTTGTTTACCAGAGGACAAACCCAAAGTTTGGCCGTAGTTACTTTAGGAACTTTATCAG
AAAGCAAAATCATTGATGATTTGAGCGGAGAAAGCAACAAACGTTTTATGTTACATTATAATTTCCCTCCGTTTGCGGTA
GGTTCTATAGGTCGTTATGCTGCTCCTAGTAGAAGAGAAATTGGTCATGGTAATTTAGCTGAAAAAGCTATTTTGCCACT
TTTACCTGAAGAAAATGATTTTCCTTACGCCATTAGAGTAGTTTCTGAAATTTTAGAATCTAATGGTTCATCTTCACAAG
CGACTGTTTGTGCCACTTCTATGTCTTTAATGGCTGCAGGTGTTCCTTTAAAAAGAGCTGTTTCAGGAATTGCCATGGGT
TTGTTTATGGATTCCAAAACTAACGAATATGTTATTTTAAGTGATATTCAAGGATTAGAAGACCATATTGGTGACATGGA
TTTAAAAATAGCAGGTAGTGATAAAGGGATTACAGCTTTGCAAATGGATCTTAAAATTGAAGGCATTTCCCAAGCTATTT
TAAAACAAGCTTTTTTTCAAGCCAAAAAAGGAAGGCTTCATATTCTAGAACATATGAATAAAACTATTGCTTCCCCGCGT
AAAGAAATGTCTCAATACGCTCCCAAAGTTCAAATGTTTCAAATAAAGCCAGAAAAAATTCGCGATGTAATCGGCTCTGC
AGGAAAAATCATTAATCAAATCATCGAAAACCATGATGGCGTTAAAATCGATATCGAGCAAGACGGCCGTATTTTTGTTA
TGCATTCTAATTTAGAAACAGTTAAACAAGCAATTCTTTTTATTAAGAATTTAATTCAAGACGCGGAAGTGAATAGTATT
TATCACGCTCATATTTCGCGTTTCTTAAATGATAAAGCTGGCAATATTTTGGGAGCTTTTGCCCAAGTGAGTCCAAGCAT
TGAAGGATTAATTCGTTTTTCTAAAGCGAAAAAAGAAAATGATGTGGTGAAAATAGGTGACAAAGTTTTGGTAAAATGTG
TTAAAATCAATGAAAGAGGGCGAATTGATTTCGTTCTTATATCAAAAAAATAA

Upstream 100 bases:

>100_bases
ATAAGCTAGTTTGTTGAAAGCGGTTCAAACACTAGCTTTTTATTTTTATATATTTTTTTCAACAAATACATTATAAAACC
CATTAAAAGGAGCATTAAAG

Downstream 100 bases:

>100_bases
ATTTACAGATTATAATAATTTTTTTATTTTTTTAGTTAAATTAAAAAAATATGGTGCTATGAGGTATGGATAAAAATTTG
AATAGTTTAAATATAACTAA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 710; Mature: 710

Protein sequence:

>710_residues
MLKQVFETTDLKNSFQVEIGTYARNVDPSVLIRFQDTVVLTTTVFSNKKNNFDFLPLTVIYQEKFYAAGKIPGSFLRREG
RSTDHEILSSRLIDRSLRPLFPKEFRQEIQVINTVLSSNPDFKSEIASILGSSLSLLISEIPFFEPVAGVYVAYIQNQFV
INPNAQQLTNSPLHLLVAGTKKNVVMIEAHASEVSEEMFLEAIVFAHEYIKKLCLFQEDVQKKVGQTKKLIDLDAELQSL
EKEFNEQYHEQTRTLVANVFEQDQKNDLQVFKKKILAKAQQKAFVKTIDQITFFDVEEQKNYLLLIENLFQKLFNQEMRN
YIIKNKKRPDKRTLEEVRNLDSQIDLLPRPHGSALFTRGQTQSLAVVTLGTLSESKIIDDLSGESNKRFMLHYNFPPFAV
GSIGRYAAPSRREIGHGNLAEKAILPLLPEENDFPYAIRVVSEILESNGSSSQATVCATSMSLMAAGVPLKRAVSGIAMG
LFMDSKTNEYVILSDIQGLEDHIGDMDLKIAGSDKGITALQMDLKIEGISQAILKQAFFQAKKGRLHILEHMNKTIASPR
KEMSQYAPKVQMFQIKPEKIRDVIGSAGKIINQIIENHDGVKIDIEQDGRIFVMHSNLETVKQAILFIKNLIQDAEVNSI
YHAHISRFLNDKAGNILGAFAQVSPSIEGLIRFSKAKKENDVVKIGDKVLVKCVKINERGRIDFVLISKK

Sequences:

>Translated_710_residues
MLKQVFETTDLKNSFQVEIGTYARNVDPSVLIRFQDTVVLTTTVFSNKKNNFDFLPLTVIYQEKFYAAGKIPGSFLRREG
RSTDHEILSSRLIDRSLRPLFPKEFRQEIQVINTVLSSNPDFKSEIASILGSSLSLLISEIPFFEPVAGVYVAYIQNQFV
INPNAQQLTNSPLHLLVAGTKKNVVMIEAHASEVSEEMFLEAIVFAHEYIKKLCLFQEDVQKKVGQTKKLIDLDAELQSL
EKEFNEQYHEQTRTLVANVFEQDQKNDLQVFKKKILAKAQQKAFVKTIDQITFFDVEEQKNYLLLIENLFQKLFNQEMRN
YIIKNKKRPDKRTLEEVRNLDSQIDLLPRPHGSALFTRGQTQSLAVVTLGTLSESKIIDDLSGESNKRFMLHYNFPPFAV
GSIGRYAAPSRREIGHGNLAEKAILPLLPEENDFPYAIRVVSEILESNGSSSQATVCATSMSLMAAGVPLKRAVSGIAMG
LFMDSKTNEYVILSDIQGLEDHIGDMDLKIAGSDKGITALQMDLKIEGISQAILKQAFFQAKKGRLHILEHMNKTIASPR
KEMSQYAPKVQMFQIKPEKIRDVIGSAGKIINQIIENHDGVKIDIEQDGRIFVMHSNLETVKQAILFIKNLIQDAEVNSI
YHAHISRFLNDKAGNILGAFAQVSPSIEGLIRFSKAKKENDVVKIGDKVLVKCVKINERGRIDFVLISKK
>Mature_710_residues
MLKQVFETTDLKNSFQVEIGTYARNVDPSVLIRFQDTVVLTTTVFSNKKNNFDFLPLTVIYQEKFYAAGKIPGSFLRREG
RSTDHEILSSRLIDRSLRPLFPKEFRQEIQVINTVLSSNPDFKSEIASILGSSLSLLISEIPFFEPVAGVYVAYIQNQFV
INPNAQQLTNSPLHLLVAGTKKNVVMIEAHASEVSEEMFLEAIVFAHEYIKKLCLFQEDVQKKVGQTKKLIDLDAELQSL
EKEFNEQYHEQTRTLVANVFEQDQKNDLQVFKKKILAKAQQKAFVKTIDQITFFDVEEQKNYLLLIENLFQKLFNQEMRN
YIIKNKKRPDKRTLEEVRNLDSQIDLLPRPHGSALFTRGQTQSLAVVTLGTLSESKIIDDLSGESNKRFMLHYNFPPFAV
GSIGRYAAPSRREIGHGNLAEKAILPLLPEENDFPYAIRVVSEILESNGSSSQATVCATSMSLMAAGVPLKRAVSGIAMG
LFMDSKTNEYVILSDIQGLEDHIGDMDLKIAGSDKGITALQMDLKIEGISQAILKQAFFQAKKGRLHILEHMNKTIASPR
KEMSQYAPKVQMFQIKPEKIRDVIGSAGKIINQIIENHDGVKIDIEQDGRIFVMHSNLETVKQAILFIKNLIQDAEVNSI
YHAHISRFLNDKAGNILGAFAQVSPSIEGLIRFSKAKKENDVVKIGDKVLVKCVKINERGRIDFVLISKK

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=660, Percent_Identity=35.6060606060606, Blast_Score=384, Evalue=1e-106,
Organism=Escherichia coli, GI145693187, Length=701, Percent_Identity=40.5135520684736, Blast_Score=538, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI115534063, Length=651, Percent_Identity=32.258064516129, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI281362905, Length=648, Percent_Identity=35.0308641975309, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24651641, Length=648, Percent_Identity=35.0308641975309, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24651643, Length=648, Percent_Identity=35.0308641975309, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI161079377, Length=601, Percent_Identity=34.9417637271215, Blast_Score=344, Evalue=1e-94,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_PHYAS (B1VAN5)

Other databases:

- EMBL:   AM422018
- RefSeq:   YP_001799249.1
- ProteinModelPortal:   B1VAN5
- SMR:   B1VAN5
- GeneID:   6799005
- GenomeReviews:   AM422018_GR
- HOGENOM:   HBG382411
- OMA:   KRVDNVD
- ProtClustDB:   PRK11824
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF01138 RNase_PH; PF03725 RNase_PH_C; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 80157; Mature: 80157

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKQVFETTDLKNSFQVEIGTYARNVDPSVLIRFQDTVVLTTTVFSNKKNNFDFLPLTVI
CCHHHHHHHCCCCCEEEEEECHHCCCCCEEEEEEECEEEEEEEEECCCCCCCCEEEEEEE
YQEKFYAAGKIPGSFLRREGRSTDHEILSSRLIDRSLRPLFPKEFRQEIQVINTVLSSNP
EEHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
DFKSEIASILGSSLSLLISEIPFFEPVAGVYVAYIQNQFVINPNAQQLTNSPLHLLVAGT
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCHHHHCCCCEEEEEECC
KKNVVMIEAHASEVSEEMFLEAIVFAHEYIKKLCLFQEDVQKKVGQTKKLIDLDAELQSL
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
EKEFNEQYHEQTRTLVANVFEQDQKNDLQVFKKKILAKAQQKAFVKTIDQITFFDVEEQK
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCC
NYLLLIENLFQKLFNQEMRNYIIKNKKRPDKRTLEEVRNLDSQIDLLPRPHGSALFTRGQ
CEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHCCCCCCCCCCEEEECCC
TQSLAVVTLGTLSESKIIDDLSGESNKRFMLHYNFPPFAVGSIGRYAAPSRREIGHGNLA
CCEEEEEEEECCCHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHH
EKAILPLLPEENDFPYAIRVVSEILESNGSSSQATVCATSMSLMAAGVPLKRAVSGIAMG
HHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
LFMDSKTNEYVILSDIQGLEDHIGDMDLKIAGSDKGITALQMDLKIEGISQAILKQAFFQ
EEEECCCCCEEEEECCCCHHHHCCCCEEEEECCCCCCEEEEEEEEECHHHHHHHHHHHHH
AKKGRLHILEHMNKTIASPRKEMSQYAPKVQMFQIKPEKIRDVIGSAGKIINQIIENHDG
HCCCCHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCC
VKIDIEQDGRIFVMHSNLETVKQAILFIKNLIQDAEVNSIYHAHISRFLNDKAGNILGAF
EEEEEECCCEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHH
AQVSPSIEGLIRFSKAKKENDVVKIGDKVLVKCVKINERGRIDFVLISKK
HHCCCCHHHHHHHHHHCCCCCEEEECHHHHHHHEEECCCCCEEEEEEECC
>Mature Secondary Structure
MLKQVFETTDLKNSFQVEIGTYARNVDPSVLIRFQDTVVLTTTVFSNKKNNFDFLPLTVI
CCHHHHHHHCCCCCEEEEEECHHCCCCCEEEEEEECEEEEEEEEECCCCCCCCEEEEEEE
YQEKFYAAGKIPGSFLRREGRSTDHEILSSRLIDRSLRPLFPKEFRQEIQVINTVLSSNP
EEHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
DFKSEIASILGSSLSLLISEIPFFEPVAGVYVAYIQNQFVINPNAQQLTNSPLHLLVAGT
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCHHHHCCCCEEEEEECC
KKNVVMIEAHASEVSEEMFLEAIVFAHEYIKKLCLFQEDVQKKVGQTKKLIDLDAELQSL
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
EKEFNEQYHEQTRTLVANVFEQDQKNDLQVFKKKILAKAQQKAFVKTIDQITFFDVEEQK
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCC
NYLLLIENLFQKLFNQEMRNYIIKNKKRPDKRTLEEVRNLDSQIDLLPRPHGSALFTRGQ
CEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHCCCCCCCCCCEEEECCC
TQSLAVVTLGTLSESKIIDDLSGESNKRFMLHYNFPPFAVGSIGRYAAPSRREIGHGNLA
CCEEEEEEEECCCHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHH
EKAILPLLPEENDFPYAIRVVSEILESNGSSSQATVCATSMSLMAAGVPLKRAVSGIAMG
HHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
LFMDSKTNEYVILSDIQGLEDHIGDMDLKIAGSDKGITALQMDLKIEGISQAILKQAFFQ
EEEECCCCCEEEEECCCCHHHHCCCCEEEEECCCCCCEEEEEEEEECHHHHHHHHHHHHH
AKKGRLHILEHMNKTIASPRKEMSQYAPKVQMFQIKPEKIRDVIGSAGKIINQIIENHDG
HCCCCHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCC
VKIDIEQDGRIFVMHSNLETVKQAILFIKNLIQDAEVNSIYHAHISRFLNDKAGNILGAF
EEEEEECCCEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHH
AQVSPSIEGLIRFSKAKKENDVVKIGDKVLVKCVKINERGRIDFVLISKK
HHCCCCHHHHHHHHHHCCCCCEEEECHHHHHHHEEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA