Click here to switch to the map view.

The map label for this gene is miaA

Identifier: 197294680

GI number: 197294680

Start: 636759

End: 637631

Strand: Reverse

Name: miaA

Synonym: PAa_0638

Alternate gene names: 197294680

Gene position: 637631-636759 (Counterclockwise)

Preceding gene: 197294681

Following gene: 197294679

Centisome position: 72.46

GC content: 29.9

Gene sequence:

>873_bases
ATGAAAAAAATAATCGCTATTACAGGCCCTACAGCTTCAGGAAAAACAAGTCTTTCTATTAAAATCGCCAAAAAATTTAA
TTTAGAAATAATTAATTGTGATTCTACTCAAATTTATCAGCAATATAATATTGGAACTGCAAAAATTACAAAAGAAGAAA
TGGAAGGAGTTAAACACCACCTTTTAGATTTTCTTCCTCCTGAAGAAAAATACAGTATTTATCATTTTCAAAAAAGTGCA
CGCGCAAAAATTAAAGATATCAACAATCCTTTGTTTGTGGGAGGAAGCGGCCTTTATTTAAAAGCAGCTCTTTTTGATTA
TGAATTAACCCCCAAGCCTTTAATGCCGCAAAATGTGTGCGAACAAGATTTACAAAAAATGCTTGCGATTATCAAAGAAA
AAGACCCTCAATTAGTTTTGGATACCAAAAACCCCCTTCGTATTATTAGTTCTTATTATGATATTGTTTCAGGTCATTTA
AGGTCTCAAAAAACTAAAAAAAATGTCCCTTTATATTCACTTTTAATTTTTTATCTTGATATTGATCGCAAAGAATTGAA
AAACAGGGTTGTTTTAAGGCTAGAAAAGATGTTAAACGAAGGTTTTGTTGAAGAAGTAAAAAACATTCAAACTAATTTCC
CCCAAGCTAATTTTAACATTATTGGTTATCGCGAAATTAAAGATTTTTTAGAGGGAAAATATAGTATAACAGAGGCTAAA
AACAAAATTTGTCAAAAGACGATGCAATACGCTAAAAGACAAAAAACTTGGTTTAAAAACCAATTGCAACCGGTTGTTGT
AGATGCTTTATCCCCTTCTTTAGAAAAAAAAGTTTTTCAATTAGTCTTTGATTTTTTGCACAAAAGGAGTTAA

Upstream 100 bases:

>100_bases
TTTGAAAAAATAATAAAATTCATATCTATTTCTTTTTTTGAAAATAATTATTTAAAACCATCTTTAAGATCCTTTAAGAT
ATACGACAAAAGGAATTATT

Downstream 100 bases:

>100_bases
AATATGATTAATACAAATGATTTTAAAACAGGTCAAACAATTAAATTTAACAATCAAATTTATCAAATTATTGAGTTTTT
ACACGTAAAACCTGGAAAAG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MKKIIAITGPTASGKTSLSIKIAKKFNLEIINCDSTQIYQQYNIGTAKITKEEMEGVKHHLLDFLPPEEKYSIYHFQKSA
RAKIKDINNPLFVGGSGLYLKAALFDYELTPKPLMPQNVCEQDLQKMLAIIKEKDPQLVLDTKNPLRIISSYYDIVSGHL
RSQKTKKNVPLYSLLIFYLDIDRKELKNRVVLRLEKMLNEGFVEEVKNIQTNFPQANFNIIGYREIKDFLEGKYSITEAK
NKICQKTMQYAKRQKTWFKNQLQPVVVDALSPSLEKKVFQLVFDFLHKRS

Sequences:

>Translated_290_residues
MKKIIAITGPTASGKTSLSIKIAKKFNLEIINCDSTQIYQQYNIGTAKITKEEMEGVKHHLLDFLPPEEKYSIYHFQKSA
RAKIKDINNPLFVGGSGLYLKAALFDYELTPKPLMPQNVCEQDLQKMLAIIKEKDPQLVLDTKNPLRIISSYYDIVSGHL
RSQKTKKNVPLYSLLIFYLDIDRKELKNRVVLRLEKMLNEGFVEEVKNIQTNFPQANFNIIGYREIKDFLEGKYSITEAK
NKICQKTMQYAKRQKTWFKNQLQPVVVDALSPSLEKKVFQLVFDFLHKRS
>Mature_290_residues
MKKIIAITGPTASGKTSLSIKIAKKFNLEIINCDSTQIYQQYNIGTAKITKEEMEGVKHHLLDFLPPEEKYSIYHFQKSA
RAKIKDINNPLFVGGSGLYLKAALFDYELTPKPLMPQNVCEQDLQKMLAIIKEKDPQLVLDTKNPLRIISSYYDIVSGHL
RSQKTKKNVPLYSLLIFYLDIDRKELKNRVVLRLEKMLNEGFVEEVKNIQTNFPQANFNIIGYREIKDFLEGKYSITEAK
NKICQKTMQYAKRQKTWFKNQLQPVVVDALSPSLEKKVFQLVFDFLHKRS

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=310, Percent_Identity=25.1612903225806, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1790613, Length=285, Percent_Identity=32.6315789473684, Blast_Score=129, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25144712, Length=112, Percent_Identity=39.2857142857143, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324848, Length=302, Percent_Identity=29.1390728476821, Blast_Score=112, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24649830, Length=108, Percent_Identity=41.6666666666667, Blast_Score=94, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_PHYAS (B1VAJ9)

Other databases:

- EMBL:   AM422018
- RefSeq:   YP_001799221.1
- ProteinModelPortal:   B1VAJ9
- SMR:   B1VAJ9
- GeneID:   6798920
- GenomeReviews:   AM422018_GR
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   CLSK343719
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33600; Mature: 33600

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIIAITGPTASGKTSLSIKIAKKFNLEIINCDSTQIYQQYNIGTAKITKEEMEGVKHH
CCEEEEEECCCCCCCCEEEEEEEEEECEEEEECCCHHHHHHHCCCHHEECHHHHHHHHHH
LLDFLPPEEKYSIYHFQKSARAKIKDINNPLFVGGSGLYLKAALFDYELTPKPLMPQNVC
HHHCCCCCCCEEHEEEHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCCCCCCCHHHH
EQDLQKMLAIIKEKDPQLVLDTKNPLRIISSYYDIVSGHLRSQKTKKNVPLYSLLIFYLD
HHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
IDRKELKNRVVLRLEKMLNEGFVEEVKNIQTNFPQANFNIIGYREIKDFLEGKYSITEAK
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCCCHHHHHH
NKICQKTMQYAKRQKTWFKNQLQPVVVDALSPSLEKKVFQLVFDFLHKRS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKIIAITGPTASGKTSLSIKIAKKFNLEIINCDSTQIYQQYNIGTAKITKEEMEGVKHH
CCEEEEEECCCCCCCCEEEEEEEEEECEEEEECCCHHHHHHHCCCHHEECHHHHHHHHHH
LLDFLPPEEKYSIYHFQKSARAKIKDINNPLFVGGSGLYLKAALFDYELTPKPLMPQNVC
HHHCCCCCCCEEHEEEHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCCCCCCCHHHH
EQDLQKMLAIIKEKDPQLVLDTKNPLRIISSYYDIVSGHLRSQKTKKNVPLYSLLIFYLD
HHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
IDRKELKNRVVLRLEKMLNEGFVEEVKNIQTNFPQANFNIIGYREIKDFLEGKYSITEAK
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCCCHHHHHH
NKICQKTMQYAKRQKTWFKNQLQPVVVDALSPSLEKKVFQLVFDFLHKRS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA