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The map label for this gene is map [H]

Identifier: 197294609

GI number: 197294609

Start: 572526

End: 573269

Strand: Reverse

Name: map [H]

Synonym: PAa_0565

Alternate gene names: 197294609

Gene position: 573269-572526 (Counterclockwise)

Preceding gene: 197294610

Following gene: 197294608

Centisome position: 65.15

GC content: 32.12

Gene sequence:

>744_bases
ATGATTTCTATCAAAACTCCTTATGAAATCTCTTTGATGAGGCAATCCGGGATTATTTTGCAACAAACTCACAAAATGCT
CGCTCCCTTTATTATTCCAGGAACTTCAACTGAAAAAATGGATTTATTAGCTCAAGAATTTTTTAGAAAAAACAACGTTG
TTTCTGCTTTTAAAGGCTATCGTGGTTTTCCAAAACACATTTGCACTTCTATTAATGAAGTAGTTGTTCATGGCATTCCT
TCCAAAAAAGCAATTTTGAAATTGGGAGATATTGTTACTTTAGATATAGGTATTAATTACCAAGGTTATTATACCGATTG
TGCTTATACTTATTTTGTAGGTAATGAATTTTTAACTCCTCCTAAAATTGTTGCCTTAACAAAAGAGGCTTTAATGCAAG
GACTTGCCCAAATTAAACCAGGAAACCATTTTTCTGATATTTCTCATGCTATCGAAACATTTGCTAAGAAACATCAATTA
GGAATTGTTCAAGATTTTACTGGTCATGGAATTGGTGCATCTTTGCATGAAGAACCTTATATTCCTAATTTTGGTCCACC
ACATCGAGGCGAACTTTTGCAAAAAGGAATGACTTTTTGTGTTGAACCTATGTTAACATTAGGAAGTTCAGAAGTTGAAG
TTTTAACTGATAATTGGACTGTTGTAACAATTGACAAAAGTTTAAGTGCTCATTTTGAACATACAGTTGTAGTTACTGAT
ACAAGTTATGAAATTTTAACCTAA

Upstream 100 bases:

>100_bases
AGTATTACCGCTCCAAAAAACAACTTTTTGAAGTTGATGGGATGCAAAACATTCAACAAGTTACTCAAAAAATTTTAAAA
GTTTTAGAAACGGAGCAAAA

Downstream 100 bases:

>100_bases
TGTTAAAAAAATTAAGAAATTAAAGGATTTTTAAATGTCAAAAACAAATATTATTGAATTGGAAGCAATTATTGTTGAAG
TATTACCCAATGCTAAATTT

Product: Methionine aminopeptidase (MAP)

Products: NA

Alternate protein names: MAP 1; Peptidase M [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MISIKTPYEISLMRQSGIILQQTHKMLAPFIIPGTSTEKMDLLAQEFFRKNNVVSAFKGYRGFPKHICTSINEVVVHGIP
SKKAILKLGDIVTLDIGINYQGYYTDCAYTYFVGNEFLTPPKIVALTKEALMQGLAQIKPGNHFSDISHAIETFAKKHQL
GIVQDFTGHGIGASLHEEPYIPNFGPPHRGELLQKGMTFCVEPMLTLGSSEVEVLTDNWTVVTIDKSLSAHFEHTVVVTD
TSYEILT

Sequences:

>Translated_247_residues
MISIKTPYEISLMRQSGIILQQTHKMLAPFIIPGTSTEKMDLLAQEFFRKNNVVSAFKGYRGFPKHICTSINEVVVHGIP
SKKAILKLGDIVTLDIGINYQGYYTDCAYTYFVGNEFLTPPKIVALTKEALMQGLAQIKPGNHFSDISHAIETFAKKHQL
GIVQDFTGHGIGASLHEEPYIPNFGPPHRGELLQKGMTFCVEPMLTLGSSEVEVLTDNWTVVTIDKSLSAHFEHTVVVTD
TSYEILT
>Mature_247_residues
MISIKTPYEISLMRQSGIILQQTHKMLAPFIIPGTSTEKMDLLAQEFFRKNNVVSAFKGYRGFPKHICTSINEVVVHGIP
SKKAILKLGDIVTLDIGINYQGYYTDCAYTYFVGNEFLTPPKIVALTKEALMQGLAQIKPGNHFSDISHAIETFAKKHQL
GIVQDFTGHGIGASLHEEPYIPNFGPPHRGELLQKGMTFCVEPMLTLGSSEVEVLTDNWTVVTIDKSLSAHFEHTVVVTD
TSYEILT

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=246, Percent_Identity=35.3658536585366, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI40385867, Length=247, Percent_Identity=33.6032388663968, Blast_Score=149, Evalue=2e-36,
Organism=Escherichia coli, GI1786364, Length=247, Percent_Identity=40.080971659919, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1788728, Length=213, Percent_Identity=27.2300469483568, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71996291, Length=216, Percent_Identity=35.6481481481481, Blast_Score=150, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6323273, Length=240, Percent_Identity=34.1666666666667, Blast_Score=161, Evalue=9e-41,
Organism=Drosophila melanogaster, GI24583427, Length=244, Percent_Identity=37.7049180327869, Blast_Score=174, Evalue=5e-44,
Organism=Drosophila melanogaster, GI21355531, Length=239, Percent_Identity=33.4728033472803, Blast_Score=157, Evalue=6e-39,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 27458; Mature: 27458

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISIKTPYEISLMRQSGIILQQTHKMLAPFIIPGTSTEKMDLLAQEFFRKNNVVSAFKGY
CCCCCCCCEEEEEHHCCCHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHCCCHHHHHHCC
RGFPKHICTSINEVVVHGIPSKKAILKLGDIVTLDIGINYQGYYTDCAYTYFVGNEFLTP
CCCHHHHHHHHHHHHEECCCCHHHHEEECCEEEEEEECCCCEEECCEEEEEEECCCCCCC
PKIVALTKEALMQGLAQIKPGNHFSDISHAIETFAKKHQLGIVQDFTGHGIGASLHEEPY
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCC
IPNFGPPHRGELLQKGMTFCVEPMLTLGSSEVEVLTDNWTVVTIDKSLSAHFEHTVVVTD
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEEECCCCCCCCEEEEEEEC
TSYEILT
CCCEECC
>Mature Secondary Structure
MISIKTPYEISLMRQSGIILQQTHKMLAPFIIPGTSTEKMDLLAQEFFRKNNVVSAFKGY
CCCCCCCCEEEEEHHCCCHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHCCCHHHHHHCC
RGFPKHICTSINEVVVHGIPSKKAILKLGDIVTLDIGINYQGYYTDCAYTYFVGNEFLTP
CCCHHHHHHHHHHHHEECCCCHHHHEEECCEEEEEEECCCCEEECCEEEEEEECCCCCCC
PKIVALTKEALMQGLAQIKPGNHFSDISHAIETFAKKHQLGIVQDFTGHGIGASLHEEPY
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCC
IPNFGPPHRGELLQKGMTFCVEPMLTLGSSEVEVLTDNWTVVTIDKSLSAHFEHTVVVTD
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEEECCCCCCCCEEEEEEEC
TSYEILT
CCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2113521; 8635744; 9384377 [H]