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The map label for this gene is prfB [H]

Identifier: 197294594

GI number: 197294594

Start: 556691

End: 557803

Strand: Reverse

Name: prfB [H]

Synonym: PAa_0550

Alternate gene names: 197294594

Gene position: 557803-556691 (Counterclockwise)

Preceding gene: 197294595

Following gene: 197294593

Centisome position: 63.39

GC content: 29.92

Gene sequence:

>1113_bases
ATGGAAAAGTATGAAATCAACCAACATTTAGAAAACATAGCTCAAAATTTAAGAGATTTAAAAGAAGCTATTAATCTTTC
CCAAAGCTACCAAACTTTAAATCAAATTAACGAATTAATGCAAAAAAAAGATTTTTGGGATGATCCTAAAAAAGCAGTTG
CAACAAGTCAGAAATTCAATGATTTACAAGAAAAAATAAATACTTTAAAACATTTAGAAACCAACCATCAAGACTTAGTA
ACTTTTTTGGATTTAGAATCCAACCAAAATAACGAAACTGCTTTTTTGGAAGAAGAAATAAAAAAATTAATAGACGCTGT
TTTAAATTTTAAAATCAAAGTTTTTTTAAATCAACCTTATGATAATAATAATGCTATTTTAATTTTTCATCCAGGAGCAG
GCGGAACGGAATCACAAGATTGGTGTGAAATGCTTTTTAGAATGTATCAAAAATATGCTCAAAAAAAGAATTTTAAAACC
GAAATCCTTGATTATCAATCAGGTGAGGAAGCAGGCATCAAATCAGCTACTTTATTAGTCAAAGGAGCTTATGCTTATGG
TTATTTAAAAGCGGAAAAAGGCGTTCATCGTTTGGTGAGAGTTTCTCCTTTTGATACTAACGCCAAAAGGCATACTTCTT
TTGTTTCTTGTGATGTTTTGCCAGAAATCAAAGAAAAAATTAAAATTGACTTAAGGCAAGAAGATTTAAAATTTGATTTT
TTTAGAAGTAGTGGAGCAGGCGGACAACACGTAAATACTACTGATTCAGCAGTGAGAATCACTCATTTGCCAACTGGATT
AGTAGTTAGTTGCCAAAACGAACGTAGTCAAAACCAAAACCGCGAAAAAGCATTACAAATGCTAAAAATCAAATTATTTC
AATTAGCCGACAAAAAACAAAAAGAGGAACATGCCAAAAATTTCCAAGGCGAACAAAAAGAAATTGGTTGGGGTTCTCAA
ATTAGAAGTTATGTTTTTCATCCTTACAAAATGGTTAAAGACCATCGCACTAATCAAGAAATTTTTCAATTAGAACAAGT
GATGGATGGAAATATTGATGTTTTTATTAATGGATATTTAACTAAAATACCATTTGTGAAAAACAATCTTTAA

Upstream 100 bases:

>100_bases
TCAACAAGAAAAAATGATTTTGAATGATAACAATTCTGATAACAATATTACCAAAAGAAGAAGAAAAGTTCGCATTTCTA
AAAAACCTTGGAATTAGTTT

Downstream 100 bases:

>100_bases
TGTTTTCATTTTTTTGATATATTAAAATAAATAAAAAGTAATAATAAATTACTAAGAAAGTAGAGTTTTATAAATATGGC
AATTAAAATGCGTTTACAAC

Product: Protein chain release factor B

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MEKYEINQHLENIAQNLRDLKEAINLSQSYQTLNQINELMQKKDFWDDPKKAVATSQKFNDLQEKINTLKHLETNHQDLV
TFLDLESNQNNETAFLEEEIKKLIDAVLNFKIKVFLNQPYDNNNAILIFHPGAGGTESQDWCEMLFRMYQKYAQKKNFKT
EILDYQSGEEAGIKSATLLVKGAYAYGYLKAEKGVHRLVRVSPFDTNAKRHTSFVSCDVLPEIKEKIKIDLRQEDLKFDF
FRSSGAGGQHVNTTDSAVRITHLPTGLVVSCQNERSQNQNREKALQMLKIKLFQLADKKQKEEHAKNFQGEQKEIGWGSQ
IRSYVFHPYKMVKDHRTNQEIFQLEQVMDGNIDVFINGYLTKIPFVKNNL

Sequences:

>Translated_370_residues
MEKYEINQHLENIAQNLRDLKEAINLSQSYQTLNQINELMQKKDFWDDPKKAVATSQKFNDLQEKINTLKHLETNHQDLV
TFLDLESNQNNETAFLEEEIKKLIDAVLNFKIKVFLNQPYDNNNAILIFHPGAGGTESQDWCEMLFRMYQKYAQKKNFKT
EILDYQSGEEAGIKSATLLVKGAYAYGYLKAEKGVHRLVRVSPFDTNAKRHTSFVSCDVLPEIKEKIKIDLRQEDLKFDF
FRSSGAGGQHVNTTDSAVRITHLPTGLVVSCQNERSQNQNREKALQMLKIKLFQLADKKQKEEHAKNFQGEQKEIGWGSQ
IRSYVFHPYKMVKDHRTNQEIFQLEQVMDGNIDVFINGYLTKIPFVKNNL
>Mature_370_residues
MEKYEINQHLENIAQNLRDLKEAINLSQSYQTLNQINELMQKKDFWDDPKKAVATSQKFNDLQEKINTLKHLETNHQDLV
TFLDLESNQNNETAFLEEEIKKLIDAVLNFKIKVFLNQPYDNNNAILIFHPGAGGTESQDWCEMLFRMYQKYAQKKNFKT
EILDYQSGEEAGIKSATLLVKGAYAYGYLKAEKGVHRLVRVSPFDTNAKRHTSFVSCDVLPEIKEKIKIDLRQEDLKFDF
FRSSGAGGQHVNTTDSAVRITHLPTGLVVSCQNERSQNQNREKALQMLKIKLFQLADKKQKEEHAKNFQGEQKEIGWGSQ
IRSYVFHPYKMVKDHRTNQEIFQLEQVMDGNIDVFINGYLTKIPFVKNNL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=270, Percent_Identity=38.8888888888889, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI34577120, Length=348, Percent_Identity=31.8965517241379, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI166795305, Length=182, Percent_Identity=39.010989010989, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI2367172, Length=357, Percent_Identity=39.4957983193277, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI1787462, Length=243, Percent_Identity=41.9753086419753, Blast_Score=196, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=311, Percent_Identity=30.2250803858521, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=308, Percent_Identity=32.7922077922078, Blast_Score=156, Evalue=5e-39,
Organism=Drosophila melanogaster, GI19921226, Length=218, Percent_Identity=38.0733944954128, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 42960; Mature: 42960

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKYEINQHLENIAQNLRDLKEAINLSQSYQTLNQINELMQKKDFWDDPKKAVATSQKFN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
DLQEKINTLKHLETNHQDLVTFLDLESNQNNETAFLEEEIKKLIDAVLNFKIKVFLNQPY
HHHHHHHHHHHHCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCC
DNNNAILIFHPGAGGTESQDWCEMLFRMYQKYAQKKNFKTEILDYQSGEEAGIKSATLLV
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCEEEEE
KGAYAYGYLKAEKGVHRLVRVSPFDTNAKRHTSFVSCDVLPEIKEKIKIDLRQEDLKFDF
EEHHHHHHHHHHHHHHHHEEECCCCCCCHHHCCEEEECCCHHHHHHHEECCCCCCCCEEE
FRSSGAGGQHVNTTDSAVRITHLPTGLVVSCQNERSQNQNREKALQMLKIKLFQLADKKQ
EECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHCCCCCHHHHHHHHHHHHHHHHHHHH
KEEHAKNFQGEQKEIGWGSQIRSYVFHPYKMVKDHRTNQEIFQLEQVMDGNIDVFINGYL
HHHHHHCCCCCHHHCCCHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECEE
TKIPFVKNNL
EECCCCCCCC
>Mature Secondary Structure
MEKYEINQHLENIAQNLRDLKEAINLSQSYQTLNQINELMQKKDFWDDPKKAVATSQKFN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
DLQEKINTLKHLETNHQDLVTFLDLESNQNNETAFLEEEIKKLIDAVLNFKIKVFLNQPY
HHHHHHHHHHHHCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCC
DNNNAILIFHPGAGGTESQDWCEMLFRMYQKYAQKKNFKTEILDYQSGEEAGIKSATLLV
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCEEEEE
KGAYAYGYLKAEKGVHRLVRVSPFDTNAKRHTSFVSCDVLPEIKEKIKIDLRQEDLKFDF
EEHHHHHHHHHHHHHHHHEEECCCCCCCHHHCCEEEECCCHHHHHHHEECCCCCCCCEEE
FRSSGAGGQHVNTTDSAVRITHLPTGLVVSCQNERSQNQNREKALQMLKIKLFQLADKKQ
EECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHCCCCCHHHHHHHHHHHHHHHHHHHH
KEEHAKNFQGEQKEIGWGSQIRSYVFHPYKMVKDHRTNQEIFQLEQVMDGNIDVFINGYL
HHHHHHCCCCCHHHCCCHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECEE
TKIPFVKNNL
EECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9696765; 9384377; 1901557; 1408743 [H]