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The map label for this gene is tpiA

Identifier: 197294559

GI number: 197294559

Start: 507657

End: 508463

Strand: Reverse

Name: tpiA

Synonym: PAa_0514

Alternate gene names: 197294559

Gene position: 508463-507657 (Counterclockwise)

Preceding gene: 197294560

Following gene: 197294558

Centisome position: 57.78

GC content: 29.86

Gene sequence:

>807_bases
GTGAATAATCTTAAAAGAATTAAAGTTATCGCGGGAAATTGGAAAATGTATAAAGATAAAAATGAAGCTTTAGAATTTAT
TCAAAAAGTTAATTTTTCCATTCCAAACTCAAAAGAAGTTGAAACAATTATTTTTGCTCAAAGTACTTTATTAGATGTTT
TGGTTCAAAACCAAGGACCTAACCTTAAAATTGGTGCTCAAAACGCTTTTCATGAAAGCGAGGGAGCTTTTACTGGAGAA
ATTTCGCCTCTTAATTTAGTTTCTTTAGGAGTTAAATATGTTTTGTTAGGGCATAGCGAAAGAAGAGTTCTTTTTGGAGA
AACCGATCAATTAGTTAATTTAAAATTATTAAAAGCGCTTCAAAATAATTTATCTCCAGTTTTATGTTTGGGTGAAACTC
TTGAAACAAAAGAAAATAACAAAACAAAAGAATTTTTAGAAAAACAATTAACACAAGCTTTAAAAGATGTTCCACAAGAA
GATTTAGAAAAGATTCTTATTGCTTATGAACCTGTTTGGGCAATCGGAACAGGAAAAACCGCTTCCCCTCAAGAAGCTAA
TCAAACTATCAAACAAATTAGGGAAAAAGTCACAAATTTATATTCAGCTCAAGTGGTGCAAAGTTTAAAAATTCTTTATG
GTGGCTCTGTTTCTTCTAATAACGTTGAGGCTATTTTAGAACAAAATGAGATTGATGGCGTTTTAGTTGGCAAAGCTTCT
TTAGAAACTAAAGATTTTTTAAATTTTACTCAAGCAGCTGTTAAATTATCAAGCAATTGTTGTCAACATTTCGATAAAAA
ATGTTAG

Upstream 100 bases:

>100_bases
TTGGGAATATTTTTTTATTTTTCAACAACAAAACACAAATATAATTTTTCTTTTTTAAAAAGAATTTTTTTAGAAAGAAA
AATACAAAAGGAGTTATTTT

Downstream 100 bases:

>100_bases
TTTTATTCTTAATTACAATTCGAAAACAGAATTAAAATCGTTAAAATAACAAATAATTTTTTGATTTTTATTTTTAAAGA
TAACAATAAGGAGTTTTTTT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MNNLKRIKVIAGNWKMYKDKNEALEFIQKVNFSIPNSKEVETIIFAQSTLLDVLVQNQGPNLKIGAQNAFHESEGAFTGE
ISPLNLVSLGVKYVLLGHSERRVLFGETDQLVNLKLLKALQNNLSPVLCLGETLETKENNKTKEFLEKQLTQALKDVPQE
DLEKILIAYEPVWAIGTGKTASPQEANQTIKQIREKVTNLYSAQVVQSLKILYGGSVSSNNVEAILEQNEIDGVLVGKAS
LETKDFLNFTQAAVKLSSNCCQHFDKKC

Sequences:

>Translated_268_residues
MNNLKRIKVIAGNWKMYKDKNEALEFIQKVNFSIPNSKEVETIIFAQSTLLDVLVQNQGPNLKIGAQNAFHESEGAFTGE
ISPLNLVSLGVKYVLLGHSERRVLFGETDQLVNLKLLKALQNNLSPVLCLGETLETKENNKTKEFLEKQLTQALKDVPQE
DLEKILIAYEPVWAIGTGKTASPQEANQTIKQIREKVTNLYSAQVVQSLKILYGGSVSSNNVEAILEQNEIDGVLVGKAS
LETKDFLNFTQAAVKLSSNCCQHFDKKC
>Mature_268_residues
MNNLKRIKVIAGNWKMYKDKNEALEFIQKVNFSIPNSKEVETIIFAQSTLLDVLVQNQGPNLKIGAQNAFHESEGAFTGE
ISPLNLVSLGVKYVLLGHSERRVLFGETDQLVNLKLLKALQNNLSPVLCLGETLETKENNKTKEFLEKQLTQALKDVPQE
DLEKILIAYEPVWAIGTGKTASPQEANQTIKQIREKVTNLYSAQVVQSLKILYGGSVSSNNVEAILEQNEIDGVLVGKAS
LETKDFLNFTQAAVKLSSNCCQHFDKKC

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=245, Percent_Identity=38.7755102040816, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI226529917, Length=245, Percent_Identity=38.7755102040816, Blast_Score=176, Evalue=2e-44,
Organism=Escherichia coli, GI1790353, Length=249, Percent_Identity=35.7429718875502, Blast_Score=163, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17536593, Length=249, Percent_Identity=39.7590361445783, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6320255, Length=249, Percent_Identity=38.9558232931727, Blast_Score=168, Evalue=7e-43,
Organism=Drosophila melanogaster, GI28572004, Length=255, Percent_Identity=39.6078431372549, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI28572008, Length=253, Percent_Identity=39.1304347826087, Blast_Score=177, Evalue=8e-45,
Organism=Drosophila melanogaster, GI28572006, Length=253, Percent_Identity=39.1304347826087, Blast_Score=177, Evalue=8e-45,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_PHYAS (B1VA75)

Other databases:

- EMBL:   AM422018
- RefSeq:   YP_001799100.1
- ProteinModelPortal:   B1VA75
- SMR:   B1VA75
- GeneID:   6799009
- GenomeReviews:   AM422018_GR
- HOGENOM:   HBG708281
- OMA:   PIVIANW
- ProtClustDB:   PRK00042
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 29809; Mature: 29809

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 98-98 ACT_SITE 170-170 BINDING 13-13 BINDING 15-15

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLKRIKVIAGNWKMYKDKNEALEFIQKVNFSIPNSKEVETIIFAQSTLLDVLVQNQGP
CCCCEEEEEEECCEEEECCHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHCCCC
NLKIGAQNAFHESEGAFTGEISPLNLVSLGVKYVLLGHSERRVLFGETDQLVNLKLLKAL
CEEECCCCCCCCCCCCEECCCCCHHHHHHHHEEEEECCCCCEEEECCCCHHHHHHHHHHH
QNNLSPVLCLGETLETKENNKTKEFLEKQLTQALKDVPQEDLEKILIAYEPVWAIGTGKT
HHCCCEEEEECCHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCC
ASPQEANQTIKQIREKVTNLYSAQVVQSLKILYGGSVSSNNVEAILEQNEIDGVLVGKAS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEECCCCCEEEEECCC
LETKDFLNFTQAAVKLSSNCCQHFDKKC
CCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNNLKRIKVIAGNWKMYKDKNEALEFIQKVNFSIPNSKEVETIIFAQSTLLDVLVQNQGP
CCCCEEEEEEECCEEEECCHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHCCCC
NLKIGAQNAFHESEGAFTGEISPLNLVSLGVKYVLLGHSERRVLFGETDQLVNLKLLKAL
CEEECCCCCCCCCCCCEECCCCCHHHHHHHHEEEEECCCCCEEEECCCCHHHHHHHHHHH
QNNLSPVLCLGETLETKENNKTKEFLEKQLTQALKDVPQEDLEKILIAYEPVWAIGTGKT
HHCCCEEEEECCHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCC
ASPQEANQTIKQIREKVTNLYSAQVVQSLKILYGGSVSSNNVEAILEQNEIDGVLVGKAS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEECCCCCEEEEECCC
LETKDFLNFTQAAVKLSSNCCQHFDKKC
CCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA